Conceptual and quantitative categorization of wave-induced flooding impacts for pedestrians and assets in urban beaches.

Beaches combined with sloping structures are frequently the first element of defense to protect urban areas from the impact of extreme coastal flooding events. However, these structures are rarely designed for null wave overtopping discharges, accepting that waves can pass above the crest and threat exposed elements in hinterland areas, such as pedestrians, urban elements and buildings, and vehicles. To reduce risks, Early Warning Systems (EWSs) can be used to anticipate and minimize the impacts of flooding episodes on those elements. A key aspect of these systems is the definition of non-admissible discharge levels that trigger significant impacts. However, large discrepancies in defining these discharge levels and the associated impacts are found among the existing methods to assess floodings. Due to the lack of standardization, a new conceptual and quantitative four-level (from no-impact to high-impact) categorization of flood warnings (EW-Coast) is proposed. EW-Coast integrates and unifies previous methods and builds on them by incorporating field-based information. Thus, the new categorization successfully predicted the impact level on 70%, 82%, and 85% of the overtopping episodes affecting pedestrians, urban elements and buildings, and vehicles, respectively. This demonstrates its suitability to support EWSs in areas vulnerable to wave-induced flooding.

Eustigmatophyte strains with potential interest in cancer prevention and treatment: partial chemical characterization and evaluation of cytotoxic and antioxidant activity.

The interest in bioactive compounds from microalgae is increasing since they have medicinal and nutritional areas. The present work aims to evaluate the potential pharmaceutical interest of extracts from three eustigmatophyte strains from the Coimbra Collection of Algae (ACOI): Chlorobotrys gloeothece, Chlorobotrys regularis and Characiopsis aquilonaris. Antioxidant and antiproliferative activities were determined as well as chlorophyll a, carotenoid and phenolic total contents. In addition, major pigments and sterols were identified and quantified. The three strains were grown until the stationary phase and then the biomass was extracted. Antioxidant activity was measured by TEAC, DPPH and FRAP assays and antiproliferative effect was assessed by the MTT method on MCF-7, PC-3 and NHDF cells. The pigment and phenolic total contents were determined by spectrophotometry. Of these strains, C. aquilonaris showed the highest antioxidant activity measured by TEAC and FRAP assays (23.98 ± 0.01 µmol TE eq g-1 DW and 42.57 ± 0.04 µmol TE eq g-1 DW, respectively), a selective effect in reduting MCF-7 cells proliferation and a larger amount of chlorophyll a, carotenoids and phenolic content (18.40 ± 0.00 µg chlorophyll a mg-1 DW, 2.27 ± 0.00 mg carotenoids g-1 DW and 6.23 ± 0.01 mg GAE g-1 DW, respectively). A positive correlation between chlorophyll a and TEAC assay was observed, as well as between carotenoids and TEAC and FRAP assays, suggesting these compounds as important contributors to significant antioxidant activity. Violaxanthin, cholesterol and stigmasterol were present in larger amount in C. aquilonaris while C. regularis showed a higher amount of ß-carotene. These results suggest that these three ACOI eustigmatophytes are promising for applications in the improvement of human health, particularly in cancer prevention and treatment.

Storm impacts on a coupled human-natural coastal system: Resilience of developed coasts.

Human occupation of and alteration of the world's coast has transformed large stretches of it into Coupled Human-Natural Systems (CHANS) in which humans both influence and are influenced by coastal evolution. In such systems, human activity is as critical on natural resilience as processes and sediment supply derived from the natural setting. Pre- and post-storm observations of these interactions on the intensively developed Atlantic coast of the Gulf of Cádiz, (Spain and Portugal) are examined to determine natural and engineering resilience. Three case studies are used in three CHANS, showing that human interventions interact in complex ways with the natural system influencing post-storm recovery. In natural coasts, storm impact is assessed in terms of geomorphological response; on developed coasts, it is quantified as damage to infrastructure or loss of amenity. Preparedness, availability of resources, choice of response and the speed at which human agencies respond affect resilience for post-storm beach behaviour. Results show in some sites natural resilience adjusting by post-storm sediment transfers and an equilibrium morphology that may differ from pre-storm morphology; engineering resilience ensured that CHANS regained their pre-storm human infrastructure and amenity. Their management requires a fundamentally different approach to that of natural coastlines. The current immature stage of understanding of CHANS (especially the human preparedness and response components) is illustrated by the case studies presented where short-term political decisions and reactions to storms play a strong role in post-storm response. The nature and extent of many developed coasts as CHANS is slowly becoming more widely acknowledged, but to increase natural resilience and decrease vulnerability in CHANS better planning is required so that future storms are anticipated and when they happen, pre-planned human response actions are activated. Storms are an integral and inevitable element in the behaviour of coastal CHANS, not a disaster or emergency.

Performance of calves born in different periods of the calving season in intensive beef cattle farming / [Desempenho em pecuária de corte intensiva de progênies nascidas em diferentes períodos da estação de parição]

The objective of this study was to evaluate, for two years, the performance of 124 female and 105 male Braford calves born during the first and second halves of the same calving season and the effects of birth period on the development of males until slaughter, as yearlings, and of females until calving, after having mated at 13-15 months of age. Early-born females were heavier than those born late at weaning (119.3 vs 109.9kg; P<0.05), at the start of the breeding season (275.0 vs 263.0kg; P<0.05), and at the end of the breeding season (300.0 vs 289.5kg; P<0.05), in addition to being more fertile (70 vs 50% pregnancy rate). There was no difference (P>0.05) in the development of males born early in relation to those born late, except for weaning weight, which was higher in the former. Steers born early were ready for slaughter at a younger age (459.6 vs 490.1 days; P<0.05), and both groups (early- and late-born) had a body condition classified as fat (4.21 points). In intensive production systems, both male and female calves perform better if they are born during the first half of the calving season.(AU)

O objetivo deste estudo foi avaliar, por dois anos, o desempenho de 124 bezerras e 105 bezerros Braford nascidos durante a primeira e segunda metades da mesma estação, bem como os efeitos do período de nascimento no desenvolvimento dos machos até o abate e das fêmeas até o primeiro parto, após serem acasalados entre 13/15 meses de idade. As fêmeas nascidas precocemente foram mais pesadas do que as nascidas tardiamente ao desmame quando bezerras (119,3 vs. 109,9kg; P<0,05), no início da estação reprodutiva (275,0 vs. 263,0kg; P<0,05) e no final da estação reprodutiva (300,0 vs. 289,5kg; P<0,05), além de terem maior fertilidade (taxa de prenhez 70 vs. 50%). Não houve diferença (P>0,05) no desenvolvimento de bezerros nascidos precocemente em relação aos nascidos mais tardiamente, exceto no peso ao desmame, com superioridade dos primeiros. Os novilhos nascidos mais cedo ficaram prontos para o abate à idade mais jovem (459,6 vs. 490,1 dias; P<0,05), e ambos os grupos (nascidos precoce e tardiamente) tinham uma condição corporal classificada como gordura (4,21 pontos). Em sistemas de produção intensiva, os bezerros machos e fêmeas nascidos precocemente, na primeira metade da estação de parição, possuem melhor desempenho.(AU)

Eosinophilic dermatosis of hematologic malignancy: a case report.

Eosinophilic dermatosis of hematologic malignancy (EDHM) is a dermatosis characterized by tissue eosinophilia that has been previously reported as insect bite-like reaction. It is a rare condition with a wide variety of clinical presentations ranging from papules, nodules, or blisters that simulate arthropod bites, to the formation of plaques of differing sizes. We report a case of eosinophilic dermatosis of hematologic malignancy in a patient with a hematoproliferative disorder.

Suplementação gestacional na produção de lã de ovelhas e cordeiros / Gestacional supplementation in wool production of ewes and lambs

O objetivo deste estudo foi avaliar o efeito da suplementação durante diferentes períodos gestacionais sobre a produção de lã de ovelhas e cordeiros da raça Ideal. Foram utilizadas 53 ovelhas da raça Ideal, com escore corporal médio (3), inseminadas pela técnica de laparoscopia, com sêmen fresco de um único reprodutor; e seus cordeiros. Os tratamentos experimentais foram: sem suplementação (n=9); suplementação do início da gestação até 50 dias (n=11); suplementação dos 51 aos 100 dias de gestação (n=11); suplementação dos 101 aos 150 dias - final da gestação (n=11); e suplementação durante toda a gestação (n=11). A suplementação foi a 1,5% do peso corporal. Amostras de lã das ovelhas e dos cordeiros foram tomadas na região do costilhar esquerdo e enviadas ao laboratório para análises objetivas de finura de lã. As suplementações no terço final e durante toda a gestação proporcionavam os melhores resultados, com aumento de produção de lã e de peso das ovelhas. Ovelhas que receberam suplementação durante toda a gestação apresentaram maior diâmetro de fibra e peso corporal. O desempenho de lã dos cordeiros não foi influenciado pela alimentação de ovelhas durante a gestação.(AU)

The objective of this study was to evaluate the effect of supplementation during different gestational periods on the wool production of sheep and lambs of the Ideal breed. Fifty-three adult Ideal sheep were used, with a mean body score inseminated by the laparoscopy technique using fresh sperm from a single breeder and his lambs. Treatment groups: No supplementation (n=9); Supplementation in the beginning of gestation up to 50 days (n=11); Supplementation from 51 to 100 days of gestation (n=11); Supplementation from 101 to 150 days - end of gestation (n=11) ;and Supplementation throughout the gestation (n=11). The supplementation was at 1.5% of body weight. After birth, lambs were kept with their mothers in cultivated pasture of black oats and ryegrass. Wool samples from sheep and lambs were taken in the left-hand region and sent to the laboratory for objective analyzes of wool fineness. The supplementation performed in the final third and throughout the gestation provides the best results, with increased sheep weight. Ewes that received supplementation throughout pregnancy had greater fiber diameter and body weight. The wool performance of lambs was not influenced by feeding sheep during gestation.(AU)

Grazing criteria for perennial peanut (Arachis pintoi cv. Amarillo) consumed by sheep in rotational stocking / Critérios de pastejo para amendoim forrageiro (Arachis pintoi cv. Amarillo) consumido por ovelhas em sistema rotacionado

The objective of work was to study the productive profile of perennial peanut in a rotational stocking environment under different criteria. The treatments corresponded to pre-grazing height of 14 and 18cm or 95% of light interception (LI) distributed in a completely randomized design. The studied variables were forage mass, percentage of structural components, forage mass and the structural components in the lower and upper canopy strata. The entrance criterion of 18cm in height, despite having higher forage mass, presented lower percentage of leaves and higher percentage of stems and forage losses. The criteria of 14cm and 95% light interception presented similar production between them and the highest rate of forage accumulation. In all criteria, in the upper strata of pasture a higher percentage of leaves were found. The criteria of 95% LI and 14cm in height presented the best productive performances. The entry criterion of 18cm presented a higher mass of pre-grazing forage with lower percentage of dead material, but with higher forage losses, resulting from senescent leaves. Due to changes in the structural components, as grazing cycles increase, the interception of 95% of the incident light by the perennial peanut occurs at lower heights.(AU)

O objetivo do presente trabalho foi estudar o perfil produtivo do amendoim forrageiro em um ambiente rotacionado, sob diferentes critérios. Os tratamentos corresponderam à altura de pré-pastejo de 14 e 18cm ou 95% de intercepção de luz (LI), distribuídos em delineamento inteiramente ao acaso. As variáveis estudadas foram massa forrageira, porcentagem de componentes estruturais, densidade da massa forrageira e densidade dos componentes estruturais nos estratos do dossel inferior e superior. O critério de entrada de 18cm de altura, apesar de ter maior massa forrageira, apresentou menor porcentagem de folhas e maior porcentagem de hastes e perdas de forragem. Os critérios de interceptação de luz de 14cm e 95% apresentaram produção similar entre eles e a maior taxa de acumulação de forragem. Em todos os critérios, no estrato superior de pastagem, foi encontrada maior porcentagem de folhas. Os critérios de 95% LI e 14cm de altura apresentaram os melhores desempenhos produtivos. O critério de entrada de 18cm apresentou maior massa de forragem pré-pastagem com menor porcentagem de material morto, mas com maiores perdas de forragem, resultantes de folhas senescentes. Devido às mudanças nos componentes estruturais, à medida que os ciclos de pastagem aumentam, a intercepção de 95% da luz incidente pelo amendoim forrageiro ocorre em alturas mais baixas.(AU)

Eco-friendly non-biocide-release coatings for marine biofouling prevention.

Environmental concerns have been changing the way of looking for solutions to problems. The hydrosphere, together with its biosphere, has been feeling the impact of many pollutants, used for instance in the marine industry for economic reasons or lack of knowledge of their effects. In particular biocides, applied as coatings in paints, are released into the waters becoming toxic and persistent extending their action to an area far beyond the initial coated surface they should protect. In order to minimize these side effects, two biocides, Irgarol (I) and Econea (E), were covalently attached to polyurethane (PU) and foul-release silicone based (PDMS) marine paints through an isocyanate linker. Their antifouling bioactivity was better in PDMS coatings, both for single (Econea) and combined biocides (E/I ratio = 1.5) with contents lower than 0.6 wt%. The treated samples remained almost clean after more than one year immersion in the Portuguese shore of the Atlantic Ocean, and after about 24 weeks under the tropical conditions of Singapore (Fouling rate < 1%). Complementary biofilm adhesion susceptibility tests against Pseudoalteromonas tunicata D2 showed adhesion reduction higher than 90% for PU formulations containing single biocides and close to 100% for PDMS with combined biocides. The eco-toxicity assessment evidenced a low environmental impact, in accordance with the European standards. In addition, shipping field trial tests showed the best antifouling performance for the Econea-based PDMS formulations (E = 0.6 wt%), which remained clean for about nine months in open seawaters, proving the efficacy of this non-release strategy, when applied under dynamic conditions.

Amendoim forrageiro manejado com baixos resíduos de pastejo por ovinos / Perennial peanut handled with low grazing waste by sheep

O objetivo do trabalho foi estudar o comportamento produtivo do amendoim forrageiro (Arachis pintoi cv. Amarillo) sob diferentes intensidades de pastejo por ovinos em ambiente rotativo. Os tratamentos corresponderam a quatro intensidades de pastejo (muito leniente - ML, leniente - L, moderado - M e severo - S) representadas pelas alturas de resíduo de 11,2; 8,4; 5,9 e 3,2cm, que foram alocadas segundo um delineamento inteiramente ao acaso, com quatro repetições. As variáveis estudadas no pré e no pós-pastejo foram: massa de forragem, percentual de folhas e caules e índice de área foliar. Ao final do experimento, foi calculada a massa de forragem total acumulada, a taxa de acúmulo e a massa de forragem desaparecida. Os dados foram submetidos à análise de variância e ao teste de comparação de médias de Tukey (P< 0,05). Foi verificado que a intensidade de pastejo modifica a composição dos componentes morfológicos e a massa de forragem acumulada pelo amendoim forrageiro, todavia o índice de área foliar no pré-pastejo não é alterado. O amendoim forrageiro não deve ser utilizado sob pastejo intenso. Intensidades de pastejo de moderada a muito leniente proporcionam maior massa de forragem pré-pastejo e total acumulada, associada a menores intervalos entre pastejos.(AU)

This research aimed to study the productive behavior of perennial peanut (Arachis pintoi cv. Amarillo) under different grazing intensities by sheep in a rotation environment. The treatments consisted of four grazing intensity levels (very lenient - VL, lenient - L, moderate - M, and severe - S) represented by the residues 11.2; 8.4; 5.9 and 3.2cm, and allocated randomly with four replications. The variables studied in the pre-grazing and post-grazing were: forage mass, percentage of leaves and stems and leaf area index. At the end of the experiment, total accumulated forage mass, accumulation rate, and mass of missing forage were calculated. The data was submitted to a variance analysis and comparison to a Tukey's test, with a 5% significance level. Grazing intensity was found to modify the composition of morphological components and the forage mass accumulated by peanuts; however, the leaf area index in pre-grazing remained constant. Perennial peanut should not be used under intensive grazing. Grazing intensity from moderate to very lenient provides greater mass of pre-grazing forage and total accumulated forage, combined with shorter intervals between grazing.(AU)

Características produtivas da consorciação de trevo-persa e azevém submetidos a pastejo / Productive characteristics of intercropping persian clover and ryegrass submitted to grazing

Este estudo teve como objetivo avaliar o efeito de diferentes intervalos entre pastejos sobre as características produtivas de trevo-persa e azevém, consorciados e manejados sob lotação rotacionada, nos anos de 2009 e 2010. Os tratamentos consistiram em quatro intervalos entre pastejos (tempo para o surgimento de 2,5; 3,5; 4,5 e 5,5 folhas em plantas de trevo-persa), em delineamento de blocos completos ao acaso, com seis repetições. As variáveis analisadas foram: índice de área foliar, área foliar, altura do pasto e de plantas, comprimento de perfilhos/ramificações, taxa de acúmulo de matéria seca e produção de matéria seca. O índice de área foliar do dossel e do trevo-persa foi maior no intervalo de 5,5 folhas nos anos de 2009 e 2010. No ano de 2009, maiores valores de área foliar foram obtidos nos intervalos de 3,5 e 4,5 folhas e não se observou efeito dos intervalos entre pastejos sobre essa variável em 2010. Maiores intervalos entre pastejos proporcionaram maior altura do pasto e de plantas e também maior comprimento de perfilhos e ramificações. A produção de matéria seca do trevo-persa foi maior em 2010, ao passo que, para azevém, maior produção foi encontrada em 2009. A produção de matéria seca total foi maior no intervalo de 5,5 folhas. A taxa de acúmulo de matéria seca foi maior no intervalo de 5,5 folhas em 2009 e no intervalo de 2,5 folhas em 2010. As características produtivas da consorciação trevo-persa e azevém anual são modificadas pelos intervalos entre pastejos. Recomendam-se intervalos de até 3,5 folhas surgidas, em consorciação de trevo-persa e azevém anual, os quais proporcionam menor alongamento de caules associado à menor presença de material morto, o que, provavelmente, melhora a qualidade da forragem colhida.

The aim of this trial was to study the effect of different grazing intervals on productive characteristics of persian clover and ryegrass, in consortium and managed under rotational stocking during the years 2009 and 2010. Treatments were composed of four grazing intervals (time to onset of 2.5; 3.5; 4.5 and 5.5 leaves in persian clover plants) allocated in completely randomized blocks, with six replications. Leaf area index (LAI), leaf area, pasture and plants height, length of tillers/branches, rate of dry matter accumulation and dry matter yield were evaluated. The leaf area index of the canopy and the persian clover was higher in the interval of 5.5 leaves in years 2009 and 2010. In 2009, higher values of leaf area were obtained in intervals of 3.5 and 4.5 leaves and there no was effect of grazing intervals on this variable in 2010. Higher grazing intervals provided greater pasture height and plant height and also greater tiller and branch length. The dry matter production of persian clover was higher in 2010, while for ryegrass highest yields were found in 2009. Total dry matter yield was higher in the interval of 5.5 leaves. The rate of dry matter accumulation was higher in the interval of 5.5 leaves in 2009 and in the interval of 2.5 leaves in 2010. The productive characteristics of a of mixture persian clover and ryegrass are modified by the grazing intervals. It is recommended intervals up to 3.5 of appeared leaves with a mixture of persian clover and ryegrass, which provides lower elongation of stems associated with a lower presence of dead material, which probably improves the quality of the harvested forage.

Finite element analysis of stress distribution in anchor teeth in surgically assisted rapid palatal expansion.

The treatment of a transverse maxillary deficiency in skeletally mature individuals should include surgically assisted rapid palatal expansion. This study evaluated the distribution of stresses that affect the expander's anchor teeth using finite element analysis when the osteotomy is varied. Five virtual models were built and the surgically assisted rapid palatal expansion was simulated. Results showed tension on the lingual face of the teeth and alveolar bone, and compression on the buccal side of the alveolar bone. The subtotal Le Fort I osteotomy combined with intermaxillary suture osteotomy seemed to reduce the dissipation of tensions. Therefore, subtotal Le Fort I osteotomy without a step in the zygomaticomaxillary buttress, combined with intermaxillary suture osteotomy and pterygomaxillary disjunction may be the osteotomy of choice to reduce tensions on anchor teeth, which tend to move mesiobuccally (premolar) and distobuccally (molar).

Análise de mutações no gene da hemocromatose e ferritina sérica em pacientes com porfiria cutânea tardía / Investigation of mutations in the gene of haemohcromatosis and the serum levels of ferritine in patients with porphiria cutanea tarda

A porfiria cutanea tardia (PCT) é uma doença que gera fotos sensibilidade pelo acúmulo de porfirinas decorrente do metabolismo de ferro alterado. O reflexo desse erro é observado bioquimicamente através das porfirinas urinárias, ferritina sérica entre outros parâmetros. Mutações no gene HFE1 são associadas tanto ao prognóstico quanto ao desencadeamento da PCT. No presente trabalho analisamos em 11 pacientes, acompanhados no ambulatório de dermatologia do Hospital Universitário Pedro Ernestro (HUPE), os genótipos do gene HFE1 para as mutações H63D, S65C e C282Y e os níveis de ferritina sérica.Material e métodos: Foram convidados 11 pacientes diagnosticados com PCT no ambulatório de dermatologia do HUPE mediante TCLE e coletados urina e sangue para determinação bioquímica de uroporfirinas e níveis de ferro/ferritina sérica respectivamente. A amostra de sangue também foi utilizada para detecção das mutações H63D, S65C e C282Y por PCR tempo real e sequenciamento.Resultados e comentários: Em oito (72%) pacientes do estudo observamos elevada ferritina sérica, sendo seis pacientes carreadores de pelo menos um alelo da mutação D63 (54%), cinco pacientes heterozigotos (45%) e um homozigoto (9%). Não foi observada a presença das mutações S65C e C282Y em nenhum dos pacientes. Mutações do gene HFE1 tem se mostrado pertinentes ao prognóstico da PCT em diversos estudos internacionais. Entretanto, não foi possível determinar a mesma importância neste trabalho, provavelmente, devido ao reduzido número de pacientes. Contudo, o estudo demonstra que adinâmica das mutações do gene HFE1 e sua relevância na população do Rio de Janeiro diferem de outras populações acometidas pela PCT.Palavras-chave: ferritina, gene HFE1, mutações C282Y e H63D, porfiria cutánea tardía (AU)

The Porphyria Cutanea Tarda (PCT) is a disease that generates photosensitivity by the accumulation of porphyries due to the metabolism of the overload of iron amount. The consequences of such mistake can be biochemically observed through the urinary porphyries, serum ferritine, among other parameters. Mutations in the gene HFE1 have been associated not only to the prognosis, but also to the development of the PCT. In this study we had analyzed in 11 patients, assisted at the dermatological outpatient clinic of the Hospital Universitário Pedro Ernestro (HUPE), the genotypes of the geneHFE1 to the mutations H63D, S65C, and C28y, as well as the levels of serum ferritine. Material and methods: We performed our study in 11 patients diagnosed with PCT at the dermatological outpatient clinic at the HUPE, who have agreed to the Terms of Consent (TC) for the study. It had been collected both urine and blood samples to the biochemical analysis of urine-porphyries, as well as the levels of iron/serum ferritine respectively. The blood sample had also been utilized to detect mutations H63D, S65C, and C282Y by PCR in real timing, and sequencing. Results and comments: It had been observed in eight patients a high level of serum ferrite (72%); six patients had been identified as carriers of at least one focus of the mutation D63 (54%): five were heterozygous patients (45%); and one homozygous patient (9%). It had not been observed the presence of mutations S65C, and C282Y in any of the patients. Mutations of the gene HFE1 have been noticed relevant to the prognosis of the PCT in various international studies. Nevertheless, it has not been possible to determine the same relevance on our study probably due to the reduced number of patients. However, this study shows that the dynamics of the gene mutations HFE1 and its relevance in the population of shows differences among populations from other places affected by the PCT (AU)

Nódulo pigmentado asimétrico de gran tamaño en una mujer de 27 años / Large Asymmetric Pigmented Nodule in a 27-Year-Old Female

No disponible

Performance of absolute CD4+ count in predicting co-infection with human T-lymphotropic virus type 1 in antiretroviral-naive HIV-infected patients.

Early identification of patients co-infected with HIV and human T-lymphotropic virus type 1 (HTLV-1) is essential to improve care, as CD4+ T-cell counts have been revealed to be an unreliable laboratory parameter to monitor HIV infection in co-infection. Unfortunately, HTLV-1 testing is not currently available in sub-Saharan Africa. We conducted this study to determine the performance of absolute CD4+ T-cell count estimation in guiding the clinical suspicion of co-infection. A cross-sectional survey was conducted in antiretroviral-naïve HIV (AN-HIV) patients attending an HIV outpatient clinic in Maputo city, Mozambique. Seven hundred and one AN-HIV patients were enrolled in the study. The prevalence of HTLV-1 co-infection was 4.5% (95% confidence interval [CI] 3.0-6.0%). Logistic regression analysis showed that CD4+ T-cell count was an independent predictor of co-infection (P value: 0.000). The performance of absolute CD4+ T-cell counts in predicting co-infection was higher in symptomatic HIV patients when compared with asymptomatic HIV patients. The best performance was achieved with the cut-off of CD4+ count of 500 cells/mm(3), which gave sensitivity, specificity, positive and negative predictive values of 54.2%, 87.2%, 24.0% and 96.2%, respectively. In conclusion, our data provide evidence that the absolute CD4+ T-cell count is of moderate accuracy in guiding the clinical suspicion of co-infection in AN-HIV and its implementation could improve the care provided to a significant number of HIV patients in Mozambique.

Toxicidade do pericarpo da Jatropha curcas em ovinos / Jatropha curcas pericarp toxicity in sheep

O pinhão manso (Jatropha curcas) é uma planta cultivada para a produção de biocombustível. O pericarpo é um coproduto com potencial para alimentação animal, e a presença de componentes tóxicos, principalmente ésteres de forbol, pode limitar sua utilização. Assim, objetivou-se avaliar a toxicidade do pericarpo. Vinte ovinos foram distribuídos em quatro grupos - um grupo-controle, que não recebeu a planta, e três experimentais, que receberam o pericarpo nas concentrações de 15% (G15), 30% (G30) e 45% (G45), durante 23 dias. Após o 10º dia, a ingestão do pericarpo promoveu redução do consumo de alimento, diarreia, desidratação e caquexia. Todos os grupos tratados apresentaram redução na concentração de fosfatase alcalina. Animais do G30 apresentaram redução na concentração de ureia e proteínas totais e elevação de potássio e sódio. No G45, houve aumento de aspartato aminotransferase, albumina, creatinina bilirrubina indireta e total. A avaliação anatomo-histopatológica revelou ascite, hidropericárdio, congestão no trato gastrintestinal e nos pulmões, edema pulmonar, aderências à parede torácica, degeneração hepática centrolobular e das células tubulares renais, pneumonia linfo-histiocitica e enterite linfoplasmocitária e histiocítica. À análise fitoquímica, constatou-se 0,3845mg de ésteres de forbol/g de pericarpo. Conclui-se que o pericarpo de J. curcas é tóxico, não sendo recomendado para alimentação de ovinos.

Physic nut (Jatropha curcas) is a plant cultivated for biofuel production. Pericarp is a potential livestock food source by-product. However, its use may be limited due to the presence of toxic compounds, mainly phorbol esters. Thus, this study aimed to evaluate pericarp toxicity. Twenty sheep were divided in four groups, one control group which did not receive the plant and three experimental groups which received pericarp in 15% (G15), 30% (G30) and 45% (G45) concentrations for 23 days. After 10 days of treatment, pericarp ingestion produced food intake decrease, diarrhea, dehydration and loss of body condition. All treated groups showed decrease in alkaline phosphatase activity. G30 animals presented reductions in urea and total protein concentrations, and increase in potassium and sodium levels. G45 animals showed increase in serum aspartate aminotransferase activity and in albumin, creatinin, total and indirect bilirubin levels. Anatomohistopathologic findings included ascites, hydropericardium, congestion of the gastintestinal tract and lungs, pulmonary edema and adhesions in the thoracic cavity, renal tubular cells and centrilobular cytoplasmic vacuolation and lymphohistiocytic pneumonia and lymphoplasmacytic and histiocytic enteritis. On the physiochemical analysis 0.3845mg of phorbol esters/g of pericarp were detected. It is concluded that J. curcas pericarp is toxic and is not recommended for sheep feeding.

Antimicrobial activity of two techniques for arm skin disinfection of blood donors in Brazil.

OBJECTIVE: Evaluation of the antimicrobial effect of skin disinfection techniques is essential to avoid the transmission of infectious agents during blood transfusion. The aim of this study was to examine the effectiveness of two methods of arm skin disinfection used in blood donors at a Hemotherapy Center in Brazil that represents an important centre for distributing haemocomponents to many cities in the country. METHODS: Two skin disinfection techniques in 50 blood donors were evaluated. For the first arm, 10% povidone-iodine/two-stage technique was used. On the opposite arm, 0.5% chlorhexidine digluconate alcohol solution/one-stage technique was used. The swabs were seeded on three culture media: blood agar, mannitol salt agar and Mac Conkey agar. Automated bacterial classification based on biochemical tests/specific substrates was performed. Donor characteristics were collected using the computerised system of the Hemotherapy Center. RESULTS: We found that microbial reduction was significantly higher for 10% povidone-iodine technique (98.57-98.87%) when compared with 0.5% chlorhexidine technique (94.38-95.06%). The species Leuconostoc mesenteroides and Staphylococcus hominis showed resistance to both disinfection techniques. We did not find statistically significant relationships between donor characteristics and microbial reduction. CONCLUSIONS: Arm skin disinfection with 10% povidone-iodine produced better antimicrobial activity. We must acknowledge that 10% povidone-iodine technique has the limitation of being a two-stage method. However, prevention of adverse events due to bacterial contamination and transfusion reactions should be prioritised. Production of hypoallergenic and stronger antiseptics that allowed a safe one-stage disinfection technique should be encouraged in health systems, not only in Brazil but also around the world.

Evaluation of proximal caries in images resulting from different modes of radiographic digitalization.

OBJECTIVE: The aim of this study was to evaluate the performances of observers in diagnosing proximal caries in digital images obtained from digital bitewing radiographs using two scanners and four digital cameras in Joint Photographic Experts Group (JPEG) and tagged image file format (TIFF) files, and comparing them with the original conventional radiographs. METHOD: In total, 56 extracted teeth were radiographed with Kodak Insight film (Eastman Kodak, Rochester, NY) in a Kaycor Yoshida X-ray device (Kaycor X-707; Yoshida Dental Manufacturing Co., Tokyo, Japan) operating at 70 kV and 7 mA with an exposure time of 0.40 s. The radiographs were obtained and scanned by CanonScan D646U (Canon USA Inc., Newport News, VA) and Genius ColorPage HR7X (KYE Systems Corp. America, Doral, FL) scanners, and by Canon Powershot G2 (Canon USA Inc.), Canon RebelXT (Canon USA Inc.), Nikon Coolpix 8700 (Nikon Inc., Melville, NY), and Nikon D70s (Nikon Inc.) digital cameras in JPEG and TIFF formats. Three observers evaluated the images. The teeth were then observed under the microscope in polarized light for the verification of the presence and depth of the carious lesions. RESULTS: The probability of no diagnosis ranged from 1.34% (Insight film) to 52.83% (CanonScan/JPEG). The sensitivity ranged from 0.24 (Canon RebelXT/JPEG) to 0.53 (Insight film), the specificity ranged from 0.93 (Nikon Coolpix/JPEG, Canon Powershot/TIFF, Canon RebelXT/JPEG and TIFF) to 0.97 (CanonScan/TIFF and JPEG) and the accuracy ranged from 0.82 (Canon RebelXT/JPEG) to 0.91 (CanonScan/JPEG). CONCLUSION: The carious lesion diagnosis did not change in either of the file formats (JPEG and TIFF) in which the images were saved for any of the equipment used. Only the CanonScan scanner did not have adequate performance in radiography digitalization for caries diagnosis and it is not recommended for this purpose.

Development of disseminated superficial porokeratosis in a patient with complicated acute pancreatitis.

Porokeratosis is a group of hereditary or acquired diseases with abnormal epidermal keratinization. A 71-year-old man was admitted to the Surgery Department after an attack of acute pancreatitis complicated with pancreatic necrosis, which required surgical resection. Three weeks after the admission, the patient was observed by our Dermatology department with sudden onset of a generalized eruption of asymptomatic flat papules with a hyperkeratotic rim, sparing the face, palms, soles, and mucous membranes. A skin biopsy was performed at the border of a leg lesion, which disclosed the presence of cornoid lamella, confirming the clinical diagnosis of disseminated superficial porokeratosis (DSP). The skin eruption spontaneously subsided about one month after pancreatic resection. The late onset of DSP in our patient may represent a type of immunosuppression-induced porokeratosis. Possibly, the pathologic clone of keratinocytes for porokeratosis was present, but remained latent until there was a decrease in the immunological status. It is possible that this relative and transient state of immunosuppression was the result of the concurrent necrotizing pancreatitis. This case represents an unusually good outcome of DSP. To the authors best knowledge, this is the first case of DSP related to severe acute pancreatitis.

Segmental neurofibromatosis: a rare variant of a common genodermatosis.

Segmental neurofibromatosis is a rare disorder characterized by features of neurofibromatosis type 1 circumscribed to a particular body segment. This entity is considered to be the result of a somatic mosaicism and is still under-diagnosed. We report a case of segmental neurofibromatosis and give a brief and up-to-date overview of the disease.