ABSTRACT
Chirality is a property of asymmetry between an object and its mirror image. Most biomolecules and many cell types are chiral. In the left-right organizer (LRO), cilia-driven flows transfer such chirality to the body scale. However, the existence of cellular chirality within tissues remains unknown. Here, we investigate this question in Kupffer's vesicle (KV), the zebrafish LRO. Quantitative live imaging reveals that cilia populating the KV display asymmetric orientation between the right and left sides, resulting in a chiral structure, which is different from the chiral cilia rotation. This KV chirality establishment is dynamic and depends on planar cell polarity. While its impact on left-right (LR) symmetry breaking remains unclear, we show that this asymmetry does not depend on the LR signaling pathway or flow. This work identifies a different type of tissue asymmetry and sheds light on chirality genesis in developing tissues.
Subject(s)
Body Patterning , Cilia/metabolism , Zebrafish/embryology , Animals , Basal Bodies/metabolism , Organizers, Embryonic/physiology , Zebrafish Proteins/metabolismABSTRACT
Fluid flows generated by motile cilia are guiding the establishment of the left-right asymmetry of the body in the vertebrate left-right organizer. Competing hypotheses have been proposed: the direction of flow is sensed either through mechanosensation, or via the detection of chemical signals transported in the flow. We investigated the physical limits of flow detection to clarify which mechanisms could be reliably used for symmetry breaking. We integrated parameters describing cilia distribution and orientation obtained in vivo in zebrafish into a multiscale physical study of flow generation and detection. Our results show that the number of immotile cilia is too small to ensure robust left and right determination by mechanosensing, given the large spatial variability of the flow. However, motile cilia could sense their own motion by a yet unknown mechanism. Finally, transport of chemical signals by the flow can provide a simple and reliable mechanism of asymmetry establishment.
Subject(s)
Body Patterning , Cilia/physiology , Embryo, Nonmammalian/physiology , Zebrafish/physiology , Animals , Embryo, Nonmammalian/cytology , Functional Laterality , Gene Expression Regulation, Developmental , Hydrodynamics , Signal Transduction , Zebrafish/embryology , Zebrafish Proteins/metabolismABSTRACT
Left-right patterning and asymmetric morphogenesis arise from a complex set of molecular and cellular interactions that are particularly dynamic and associated with mechanical forces. How do mechanical forces translate into tissular asymmetries? Are these forces asymmetrical de novo, or do they build up from pre-existing asymmetries? Advances in developmental genetics, live imaging and cell biology have recently shed light on the origins of mechanical forces generated at the cell scale and their implication in asymmetric patterning and morphogenesis is now emerging. Here we ask when and how, molecular asymmetries and mechanical forces contribute to left-right patterning and organ asymmetries.
Subject(s)
Actomyosin/genetics , Body Patterning/genetics , Cell Polarity/genetics , Gene Expression Regulation, Developmental , Actomyosin/metabolism , Animals , Biomechanical Phenomena , Cell Adhesion , Cell Differentiation , Cell Movement , Chick Embryo , Chickens , Cilia/metabolism , Embryo, Mammalian , Embryo, Nonmammalian , Mechanotransduction, Cellular , Mice , Zebrafish/embryologyABSTRACT
Internal organs are asymmetrically positioned inside the body. Embryonic motile cilia play an essential role in this process by generating a directional fluid flow inside the vertebrate left-right organizer. Detailed characterization of how fluid flow dynamics modulates laterality is lacking. We used zebrafish genetics to experimentally generate a range of flow dynamics. By following the development of each embryo, we show that fluid flow in the left-right organizer is asymmetric and provides a good predictor of organ laterality. This was tested in mosaic organizers composed of motile and immotile cilia generated by dnah7 knockdowns. In parallel, we used simulations of fluid dynamics to analyze our experimental data. These revealed that fluid flow generated by 30 or more cilia predicts 90% situs solitus, similar to experimental observations. We conclude that cilia number, dorsal anterior motile cilia clustering, and left flow are critical to situs solitus via robust asymmetric charon expression.
Subject(s)
Cilia/physiology , Dyneins/metabolism , Embryo, Nonmammalian/metabolism , Functional Laterality , Zebrafish Proteins/metabolism , Zebrafish/metabolism , Animals , Computer Simulation , Embryo, Nonmammalian/cytology , Fluorescent Antibody Technique , Gastrointestinal Tract/cytology , Gastrointestinal Tract/metabolism , Heart/physiology , In Situ Hybridization , Models, Theoretical , Zebrafish/embryologyABSTRACT
VIDEO ABSTRACT: The pattern of blood flow has long been thought to play a significant role in vascular morphogenesis, yet the flow-sensing mechanism that is involved at early embryonic stages, when flow forces are low, remains unclear. It has been proposed that endothelial cells use primary cilia to sense flow, but this has never been tested in vivo. Here we show, by noninvasive, high-resolution imaging of live zebrafish embryos, that endothelial cilia progressively deflect at the onset of blood flow and that the deflection angle correlates with calcium levels in endothelial cells. We demonstrate that alterations in shear stress, ciliogenesis, or expression of the calcium channel PKD2 impair the endothelial calcium level and both increase and perturb vascular morphogenesis. Altogether, these results demonstrate that endothelial cilia constitute a highly sensitive structure that permits the detection of low shear forces during vascular morphogenesis.
