Effects of single- or pair-housing on the welfare of shelter dogs: Behavioral and physiological indicators.

Dogs are often housed alone in shelter settings to reduce injury and disease spread. However, social isolation can be a stressor for dogs. Prior studies have suggested that cohousing can produce behavioral and physiological benefits. These studies have typically focused on laboratory dogs or shelter dogs that have been kenneled for several months. Thus, those results might not necessarily generalize to shelter dogs, many of which have shorter lengths of stay than those dogs studied to date, and might be cohoused soon after intake. In fact, being pair-housed could, in the short term, be more stressful as dogs have to navigate novel social situations in small spaces. We investigated the behavioral and physiological effects of single- or pair-housing shelter dogs, most of which had recently entered the shelter. We collected behavioral data on 61 dogs (30 single-housed; 31 pair-housed) daily across seven days; we also collected urine for cortisol:creatinine analysis on a subset (22 single-housed; 18 pair-housed) for eight days (each day of the seven-day study plus a baseline sample on Day 0, prior to dogs' enrollment). We found pair-housed dogs engaged in three stress-related behaviors (lip licking, whining, and ears back) significantly less frequently than single-housed dogs. When we analyzed the change in urinary cortisol:creatinine (Days 1-7 values minus Day 0 value), we found that pair-housed dogs generally showed a greater decrease in cortisol:creatinine levels than single-housed dogs. Pair-housed dogs also had significantly shorter lengths of stay, but we did not detect any effect on dog-dog skills. Overall, we found well-matched pair-housing can have both proximate and ultimate welfare benefits for shelter dogs.

Factors associated with behavioral euthanasia in pet dogs.

When pet dogs demonstrate certain serious problem behaviors, this may lead owners to choose behavioral euthanasia. However, research on behavioral euthanasia of pet dogs is sparse and previously published papers have not specifically sampled owners who made the decision to euthanize for behavior. The Behavioral Euthanasia in Pet Dogs Questionnaire was created to collect a wide range of information from dog owners who made behavioral euthanasia decisions. Using this survey, we explored the types of behaviors associated with behavioral euthanasia. Human-directed aggression, especially toward adults living in the household, was the most frequently reported reason for choosing behavioral euthanasia; followed by aggression toward other animals, especially other dogs living in the same household. The majority of dogs displaying human-directed or other animal-directed aggression were reported to have bitten and broken skin, and many of these had bitten in multiple or severe incidents. Most dogs had lived in their homes and displayed problem behaviors for over a year prior to behavioral euthanasia, and the euthanasia occurred at a variety of ages, from less than 1 year old to 18 years old. Additional research is required to understand environmental or nonbehavioral factors contributing to the behavioral euthanasia of companion animals, as well as the psychosocial and emotional impact of behavioral euthanasia on the human experience. Understanding the behavioral factors associated with behavioral euthanasia can direct resources toward problem behavior interventions, improve public education about animal behavior, and strengthen the human-animal bond.

The Influence of Brief Outing and Temporary Fostering Programs on Shelter Dog Welfare.

Human interaction is one of the most consistently effective interventions that can improve the welfare of shelter-living dogs. Time out of the kennel with a person has been shown to reduce physiological measures of stress as can leaving the shelter for a night or more in a foster home. In this study, we assessed the effects of brief outings and temporary fostering stays on dogs' length of stay and outcomes. In total, we analyzed data of 1955 dogs from 51 animal shelters that received these interventions as well as 25,946 dogs residing at these shelters that served as our controls. We found that brief outings and temporary fostering stays increased dogs' likelihood of adoption by 5.0 and 14.3 times, respectively. While their lengths of stay were longer in comparison to control dogs, this difference was present prior to the intervention. Additionally, we found that these programs were more successful when greater percentages of community members (as compared to volunteers and staff) were involved in caregiving as well as when programs were implemented by better-resourced shelters. As such, animal welfare organizations should consider implementing these fostering programs as evidence-based best practices that can positively impact the outcomes of shelter dogs.

Exploring the Domestication Syndrome Hypothesis in Dogs: Pigmentation Does Not Predict Cortisol Levels.

Previous research has found connections between pigmentation, behavior, and the physiological stress response in both wild and domestic animals; however, to date, no extensive research has been devoted to answering these questions in domestic dogs. Modern dogs are exposed to a variety of stressors; one well-studied stressor is residing in an animal shelter. To explore the possible relationships between dogs' responses to stress and their pigmentation, we conducted statistical analyses of the cortisol:creatinine ratios of 208 American shelter dogs as a function of their coat color/pattern, eumelanin pigmentation, or white spotting. These dogs had been enrolled in previous welfare studies investigating the effect of interventions during which they left the animal shelter and spent time with humans. In the current investigation, we visually phenotype dogs based on photographs in order to classify their pigmentation and then conduct post hoc analyses to examine whether they differentially experience stress as a function of pigmentation. We found that the dogs did not differ significantly in their urinary cortisol:creatinine ratios based on coat color/pattern, eumelanin pigmentation, or white spotting, either while they were residing in the animal shelter or during the human interaction intervention. These preliminary data suggest that pigmentation alone does not predict the stress responses of shelter dogs; however, due to the small sample size and retrospective nature of the study, more research is needed.

The Science and Social Validity of Companion Animal Welfare: Functionally Defined Parameters in a Multidisciplinary Field.

Social validity refers to the social significance and acceptability of intervention goals, procedures, and outcomes. Animal practitioners, who are often guided by the principles of ABA, lack the benefit of verbal participants (at least with respect to target animals) with which to assess a client's needs and preferences. The study of a learner's welfare is useful for determining areas where intervention is needed or how the learner feels about an intervention that is underway. Three tenets of animal welfare measurement include physiological function, naturalistic behavior, and affect, where affect refers to private events, including emotions, which are a function of the same variables and contingencies responsible for controlling public behavior. The development of new technologies allows us to look "under the skin" and account for subjective experiences that can now be observed objectively. We introduce the reader to tools available from the animal welfare sciences for the objective measurement of social validity from the learner's perspective.

Social interaction can function as a reinforcer for dogs: Effects of stimulus duration and session parameters.

Prior research has produced mixed results on whether human social interaction can function as a reinforcer for dog behavior. However, that research used either short durations of social interaction or rapid, repeated trials such that satiation could have been a factor. We investigated whether two durations of social interaction (30 s or 4 s petting plus vocal praise) would maintain more responding than extinction, than each other, or than food. We limited each session to 10 trials and temporally spaced sessions within and across days. Both durations of social interaction produced more responding than extinction, but there was no difference in responding between the two social interaction durations. When we compared responding in food sessions to 30-s and 4-s social interaction sessions, we could not determine differences in responses emitted per session for two dogs due to ceiling effects, but the third dog doubled her responding when food was provided. Additionally, latencies in food sessions for all dogs were significantly lower than expected from a random sampling of latencies. Our results suggest both durations of social interaction can function as a reinforcer, especially when delivered sporadically, but they are still not as effective as food as a reinforcer for most dogs.

Reinforcer efficacy of grain for horses.

Positive reinforcement is becoming more common in horse training. Identifying effective reinforcers is critical for training success. The aim of this study was to determine relative reinforcer efficacy of different grains. Four horses learned to muzzle touch a target, after which they were tested using a progressive ratio schedule of reinforcement with different grains as the consequence. Break points were used to determine relative reinforcer efficacy of each grain and were also converted into unit price per kilocalorie to determine if caloric value impacted reinforcer efficacy. Condition 1 compared three textured grains that spanned industry-accepted standards of low to high nonstructural carbohydrate content. Condition 2 compared three pelleted grains that similarly differed in nonstructural carbohydrate content, comparable to Condition 1, but that had a different texture than those of Condition 1. Finally, Condition 3 directly compared one grain each from Conditions 1 and 2. Results showed overall little difference in reinforcer efficacy or unit price between grains but found that all grains tested functioned as reinforcers for the horses. This suggests that a range of commercially available grains can maintain behavior and therefore be used for training. We also identified possible extraexperimental factors that impact reinforcer efficacy.

Emergency Fostering of Dogs From Animal Shelters During the COVID-19 Pandemic: Shelter Practices, Foster Caregiver Engagement, and Dog Outcomes.

Each year, millions of dogs enter thousands of animal shelters across the United States. Life in the shelter can be stressful, and one type of intervention that improves dogs' experience is human interaction, particularly stays in foster homes. Prior research has demonstrated that fostering can reduce dogs' cortisol and increase their resting activity. Despite these benefits, little is understood about the utilization of foster caregiving in animal shelters, and even less so during a crisis. On March 11, 2020, the World Health Organization deemed the coronavirus outbreak a worldwide pandemic, and subsequently a nationwide emergency was declared in the United States. Nearly all states issued stay-at-home orders to curb the spread of the virus. During this time, media outlets reported increased interest in the adoption and fostering of shelter pets. This study explores canine foster caregiving at 19 US animal shelters during the first 4 months of the COVID-19 pandemic. In our investigation, we found that shelters' utilization of foster caregiving increased from March to April 2020 but returned to initial pandemic levels by June 2020. Slightly less than two-fifths of foster caregivers were community members with no prior relationship with the shelter, and these caregivers were over four times more likely to adopt their fostered dogs than those with a pre-existing relationship to the shelter. Individuals fostering with the intention to adopt, in fact, adopted their dogs in nearly three-quarters of those instances. With regards to shelters' available resources, we found that very low-resource shelters relied more heavily on individuals with prior relationships to provide foster caregiving while very high-resource shelters more often recruited new community members. We also found that our lowest resourced shelters transferred more dogs out of their facilities while more resourced shelters rehomed dogs directly to adopters. To our knowledge, these findings represent the first in-depth reporting about dog fostering in US animal shelters and, more specifically, foster caregiving during the COVID-19 pandemic. In total, they provide greater understanding of how monetary and human resources were utilized to affect the care and ultimately, the outcomes of shelter dogs during this time.

Dogs and wolves differ in their response allocation to their owner/caregiver or food in a concurrent choice procedure.

Dogs and wolves both show attachment-like behaviors to their owners/caregivers, including exploring more in the presence of the owner/caregiver, and greeting the owner/caregiver more effusively after an absence. Concurrent choice studies can elucidate dogs' and wolves' relationship to their owners/caregivers by assessing their preference for the owner/caregiver compared to other stimuli. While previous research has used concurrent choice paradigms to evaluate dogs' and wolves' preference between humans giving social interaction or humans giving food, no research has explored their preferences for an owner/caregiver compared to food when the food is not delivered by a human. In the current study, we investigated whether dogs and hand-reared wolves preferred their owner/caregiver or food, unassociated with a human, when they had been equally deprived of each stimulus (at least 4 hours). Each canid experienced four trials; we measured first choice and time spent with each alternative. Dogs overall did not show a preference for the owner or food. Wolves, on the other hand, tended to show a preference for food in both measures. We observed a range of individual variation in both measures, although dogs showed more individual variation. The differences we observed between dogs and wolves align with prior research comparing wolf and dog behavior directed towards humans; however, the reasons for this differential responding could be due to a variety of factors beyond phylogeny.

Habituation Protocols Improve Behavioral and Physiological Responses of Beef Cattle Exposed to Students in an Animal Handling Class.

Our objective was to determine the impact of different habituation protocols on beef cattle behavior, physiology, and temperament in response to human handling. Beef heifers were exposed to three habituation strategies: (1) tactile stimulation (brushing) in the working chute for seven consecutive days (STI; n = 18); (2) passage through the working chute for seven consecutive days (CHU; n = 19) and; (3) no habituation (CON; n = 19). Individual heifer respiratory rate (RR; n/min), internal vaginal temperature (VAGT; °C), and blood cortisol were measured. Further, behavior parameters were observed to generate a behavior score, and heifer interaction with students and their behavioral responses were recorded. Habituation with STI and CHU resulted in improved numerical behavioral scores compared to CON, and greater (p ≤ 0.05) handling latencies. Vaginal temperature was decreased in STI compared to CHU and CONT (p ≤ 0.05). Cortisol concentration did not differ among treatments, but decreased (p ≤ 0.05) from the start of the experiment to 14 days after treatment initiation. Both habituation protocols showed benefits, but heifers that received the positive tactile stimulation in the chute had the greatest behavior improvements. Furthermore, these heifers responded more calmly during student-animal interactions in class, which is beneficial for the students' and animals' safety.

Investigating the Impact of Brief Outings on the Welfare of Dogs Living in US Shelters.

Social isolation likely contributes to reduced welfare for shelter-living dogs. Several studies have established that time out of the kennel with a person can improve dogs' behavior and reduce physiological measures of stress. This study assessed the effects of two-and-a-half-hour outings on the urinary cortisol levels and activity of dogs as they awaited adoption at four animal shelters. Dogs' urine was collected before and after outings for cortisol:creatinine analysis, and accelerometer devices were used to measure dogs' physical activity. In total, 164 dogs participated in this study, with 793 cortisol values and 3750 activity measures used in the statistical analyses. We found that dogs' cortisol:creatinine ratios were significantly higher during the afternoon of the intervention but returned to pre-field trip levels the following day. Dogs' minutes of low activity were significantly reduced, and high activity significantly increased during the outing. Although dogs' cortisol and activity returned to baseline after the intervention, our findings suggest that short-term outings do not confer the same stress reduction benefits as previously shown with temporary fostering. Nevertheless, it is possible that these types of outing programs are beneficial to adoptions by increasing the visibility of dogs and should be further investigated to elucidate these effects.

Using Owner Return as a Reinforcer to Operantly Treat Separation-Related Problem Behavior in Dogs.

Separation-related problem behavior (SRPB) is a severe behavioral issue in which dogs engage in a variety of undesirable behaviors when the owner is absent, such as destructive behavior and excessive vocalization. Given the severity and high prevalence of SRPB, finding effective treatments is crucial. To date, most treatments have relied on habituation to increase tolerance to owner absence. Additionally, research has typically not utilized direct observations of the dog's behavior and the treatment implemented with unknown treatment integrity. We evaluated an operant approach to SRPB using owner return as the reinforcer. After collecting baseline, we enrolled five dogs for treatment. Treatment involved differential reinforcement of either absence of problem behavior or occurrence of specific desirable behaviors. Behavioral criteria for delivering reinforcement changed based on the dog's performance assessed through direct observation. We coached owners to ensure treatment integrity on each trial. From baseline, mean time to SRPB was 27.1 s. During treatment, all dogs increased their ability to stay alone without SRPB compared to baseline, indicating that contingent owner return can be a useful treatment. However, despite four training sessions, only one dog was able to stay alone for over 5 min. Our data demonstrate the slow-going progression of this SPRB treatment and the challenges of this behavioral issue.

Minor procedural variations affect canine behavior during sociability assessments.

A growing number of studies make claims about canine sociability in both applied and basic contexts. Yet, there is currently no standard for measuring sociability in dogs. The purpose of this two-part study was to determine whether procedural differences among canine sociability tests would affect dogs' behavior. In Experiment 1, we used a mixed-subjects design to assess whether experimenter position (standing, sitting, or kneeling) and presence of affection (petting and praise or none) affected leashed dogs' social behavior. Mixed-effect logistic regression modeling showed statistically significant main effects and interactions between posture and social contact. On average, dogs were more social when the experimenter knelt and provided social contact. However, there were individual differences in how dogs were affected by changes in procedure. In Experiment 2, we examined correspondence between leashed and unleashed dogs' social behaviors including time in proximity, following patterns, eye gaze, body orientation, jumping, and touching. Individual differences were examined by calculating a binomial mixed-effect logistic regression with condition (leashed, off-leash, following) and subject ID as fixed effects. Interactions were statistically significant for all behaviors, indicating that individual differences were present. The results have implications for the validity of sociability tests.

The stability of function of automatically reinforced vocal stereotypy over time.

Although prior research has suggested the function of socially reinforced problem behavior can change across time, the stability of the function of automatically reinforced behavior is largely unknown. Further, some authors have suggested automatically reinforced behavior is likely to enter into socially mediated contingencies. The present study compared 2 functional analyses conducted on the same target behavior at least 1 year apart. Participants were 6 individuals diagnosed with an intellectual or developmental disability displaying automatically reinforced vocal stereotypy. Results indicated the function of each participant's vocal stereotypy remained stable over time (i.e., no new functions were acquired); however, future research on functional stability for automatically reinforced behavior of other topographies is needed.

Evaluating the effects of a temporary fostering program on shelter dog welfare.

One of the greatest stressors for dogs living in animal shelters is social isolation. Many studies have demonstrated that human interaction reduces cortisol in shelter dogs, with the possibility that longer periods of interaction may yield greater effects. These types of interventions are contingent upon removing the dog from the kennel and any such reductions in cortisol are often lost when the dog returns to the kennel. More recently, animal shelters are utilizing short-term fostering programs to provide relief from the perceived stresses of kennel life; however the effects of these programs are not well understood. This study assessed the impacts of one- and two-night fostering programs on the urinary cortisol levels, resting pulse rates, longest bout of uninterrupted rest, and proportion of time spent resting of dogs awaiting adoption. Five animal shelters, open and limited-admission facilities, from across the United States participated in the study. During the study, dogs' urine was collected in the morning before, during, and after fostering stays for cortisol: creatinine analysis. Non-invasive health monitors were worn by the dogs, which collected heart rates and activity levels, in the shelter and in foster homes. In total, 207 dogs participated in the study, and 1,076 cortisol values were used in our analysis. Across all shelters, we found that dogs' cortisol: creatinine ratios dropped significantly during their fostering stay, but returned to baseline levels after return to the shelter. However, the observed reduction in cortisol varied in magnitude across shelters. We found that dogs of greater weight, age, and average resting pulse rate had higher cortisol levels; and dogs with longer bouts of uninterrupted rest had lower cortisol levels. Dogs had their longest bouts of rest during sleepovers, followed by in the shelter after their sleepovers. Lastly, significant differences were found when comparing in-shelter cortisol values at our five shelters, differences that were in some cases greater than the impact of the fostering intervention itself. Considering the diversity of facilities that participated in this study, it is possible that as yet unstudied, shelter-specific, environmental factors could be contributing to the overall welfare of shelter dogs. Thus while a reprieve from the shelter is impactful for dogs awaiting adoption, mitigating the stressors present in kenneling conditions should also be addressed to improve the lives of shelter dogs.

Dogs don't always prefer their owners and can quickly form strong preferences for certain strangers over others.

The unique relationship between dog and owner has been demonstrated in several experimental procedures, including tests in which dogs are left alone or with a stranger, tests of dogs' appeasement or social approach when petted by their owner or a stranger, and their ability to learn when taught by their owner or a stranger. In all cases, dogs responded differently to their owner, which has been referred to as a specific attachment, and likely a product of a prolonged history of reinforcement. In the current study, we used a concurrent choice paradigm in which dogs could interact with two people, both of whom provided the same petting interaction, to test whether owned dogs would prefer their owner over a stranger and whether the familiarity of the testing context would influence preference. We also investigated whether shelter and owned dogs tested with two strangers would show a preference between strangers and whether that preference would be similar in magnitude to any preference between the owner and stranger. Owned dogs preferred to interact with their owners when in an unfamiliar context, but allocated more time to the stranger in a familiar context. Both shelter and owned dogs tested with two strangers showed a magnitude of preference for one stranger over the other similar to owned dogs' preference for owners in an unfamiliar context. These results parallel what has been found in strange situation tests with owned dogs tested with their owners, but the strength of preference shown for one of two strangers indicates dogs can form a preference for one person quickly.

Application of functional analysis methods to assess human-dog interactions.

Research on owner-dog relationships suggests that they have remarkable features, paralleling those of infant to parents. In this study, we investigated whether, after being separated, access to the owner would function as a reinforcer for domestic dog behavior. We then conducted a functional analysis to determine the specific functional reinforcer (e.g., owner access, attention). Our results demonstrate that owner access can function as a reinforcer. This has implications for understanding the owner-dog relationship and using owner access as a training tool.

Shut up and pet me! Domestic dogs (Canis lupus familiaris) prefer petting to vocal praise in concurrent and single-alternative choice procedures.

The nature of the interactions that maintain the social behavior of dogs toward humans and which interactions dogs prefer have not been thoroughly investigated. We focused here on dogs' preference for petting and vocal praise, and the influence that familiarity (owner vs. stranger) has on that preference. We first used concurrent choice to evaluate dogs' preference for petting or vocal praise and measured the initial choice, the time spent with each alternative, and the number of within-session alternations. We assessed dogs' preference for petting or vocal praise in (1) shelter dogs, (2) owned dogs with strangers providing both interactions, and (3) owned dogs with the dog's owner providing the interactions. Across all experimental groups, dogs preferred petting to vocal praise. We next assessed time spent with each alternative when only one alternative was available at a time in shelter dogs and owned dogs (Experiment 2). Shelter dogs were tested with a stranger and owned dogs were tested with their owners providing the interaction. Dogs alternated between petting and vocal praise, vocal praise and no interaction, or received only petting for eight 3-min sessions of each comparison. Both shelter and owned dogs spent significantly longer in proximity to the experimenter when the interaction was petting compared to vocal praise. Vocal praise produced as little proximity-seeking behavior as did no interaction. Additionally, dogs did not show any sign of satiation with petting across all eight sessions. Overall, petting seems to be an important interaction between dogs and humans that might maintain inter-specific social behavior but vocal praise likely has to be specifically conditioned. This article is part of a Special Issue entitled: Canine Behavior.

Most domestic dogs (Canis lupus familiaris) prefer food to petting: population, context, and schedule effects in concurrent choice.

Previous research has indicated both petting (McIntire & Colley, 1967) and food (Feuerbacher & Wynne, 2012) have reinforcing effects on dog behavior and support social behavior towards humans (food: Elliot & King, 1960; social interaction: Brodbeck, 1954). Which type of interaction dogs prefer and which might produce the most social behavior from a dog has not been investigated. In the current study, we assessed how dogs allocated their responding in a concurrent choice between food and petting. Dogs received five 5-min sessions each. In Session 1, both food and petting were continuously delivered contingent on the dog being near the person providing the respective consequence. Across the next three sessions, we thinned the food schedule to a Fixed Interval (FI) 15-s, FI 1-min, and finally extinction. The fifth session reversed back to the original food contingency. We tested owned dogs in familiar (daycare) and unfamiliar (laboratory room) environments, and with their owner or a stranger as the person providing petting. In general, dogs preferred food to petting when food was readily available and all groups showed sensitivity to the thinning food schedule by decreasing their time allocation to food, although there were group and individual differences in the level of sensitivity. How dogs allocated their time with the petting alternative also varied. We found effects of context, familiarity of the person providing petting, and relative deprivation from social interaction on the amount of time dogs allocated to the petting alternative.

Relative efficacy of human social interaction and food as reinforcers for domestic dogs and hand-reared wolves.

Despite the intimate relationship dogs share with humans in Western society, we know relatively little about the variables that produce and maintain dog social behavior towards humans. One possibility is that human social interaction is itself a reinforcer for dog behavior. As an initial assessment of the variables that might maintain dog social behavior, we compared the relative efficacy of brief human social interaction to a small piece of food as a reinforcer for an arbitrary response (nose touch). We investigated this in three populations of canids: shelter dogs, owned dogs, and hand-reared wolves. Across all three canid populations, brief social interaction was a relatively ineffective reinforcer compared to food for most canids, producing lower responding and longer latencies than food.