Sensitivity and specificity of a tail-activity measuring device for calving prediction in dairy cattle.

Efficient calving surveillance is essential for avoiding stillbirth due to unattended dystocia. Calving sensors can help detect the onset of parturition and thus ensure timely calving assistance if necessary. Tail-raising is an indicator of imminent calving. The objective of this study was to evaluate a tail-mounted inclinometer sensor (Moocall Ltd., Dublin, Ireland) and to monitor skin integrity after sensor attachment. Cows (n = 157) and heifers (n = 23) were enrolled at 275 d post insemination, and a sensor was attached to each cow's tail. Investigators checked for signs indicating the onset of stage II of parturition, verified the position of the sensor, and evaluated the skin integrity of the tail above and below the sensor hourly for 24 h/d. We used 5 different intervals (i.e., 1, 2, 4, 12, and 24 h until calving) to calculate sensitivity and specificity. Sensors continuously remained on the tail (i.e., within 3 cm of the initial attachment position) after initial attachment until the onset of calving in only 13.9% of animals (n = 25). Sensors were reattached until a calving event occurred (51.6%) or the animal was excluded for other reasons (34.4%). In 31 animals the sensor was removed because the tail was swollen or painful. Heifers were significantly less likely than cows to lose a sensor but more likely to experience tail swelling or pain. Depending on the interval preceding the onset of parturition, sensitivity varied from 19 to 75% and specificity from 63 to 96%.

Influence of denaverine hydrochloride on calving ease in Holstein-Friesian heifers.

Calving is assumed to be an exhausting and painful event. A drug that eases the calving procedure and alleviates pain would help cows, especially those suffering from dystocia. In a randomized, controlled, and blinded trial, we measured the effect of denaverine hydrochloride on physical and physiological calving parameters. Eighty-three Holstein-Friesian heifers were included in the analysis. Pulling force was measured using a digital force gauge interposed between the calf and a mechanical calf puller. The concentration of cortisol was measured in serum before and after parturition. There was no effect of treatment group on calving modality (i.e., spontaneous vs. assisted calving), duration of calving, and cortisol concentration. The area under the curve of pulling force × time (n = 44), however, was significantly smaller in the treatment group compared with the placebo group. Also, duration of calving assistance was numerically shorter in the treatment group compared with the placebo group. The results provide evidence that calving ease can be influenced by denaverine hydrochloride during calving assistance.

Short communication: Is hair cortisol a potential indicator for stress caused by chronic lameness in dairy cows?

The objective of this study was to evaluate hair cortisol concentration as an indicator for stress caused by chronic lameness in dairy cows. Sixty-eight cows were scored for lameness for 4 consecutive weeks. The hair of the tail switch was clipped at the beginning of the study and regrown hair was clipped after 4 wk. Hair samples were analyzed for cortisol concentration. Animals with 2 consecutive locomotion scores ≥3 or with an overall mean score >1.5 were classified as lame. After pair matching lame and nonlame cows, considering days in milk, lactation number, and milk yield, and excluding cows with less than 20 mg hair sample for analysis, 21 lame and 21 nonlame cows were included in the analysis. The mean hair cortisol concentration in this study was 2.32 ± 0.35 pg/mg (mean ± standard deviation). Cortisol concentration from hair regrown in the study period was 2.38 ± 0.95 and 2.26 ± 1.35 pg/mg for lame and nonlame cows (n = 21), respectively; we found no difference in mean cortisol level of primiparous and multiparous cows. Based on these data, hair cortisol concentration was not a useful indicator to differentiate cows with chronic lameness and healthy cows.

Detecting Staphylococcus aureus in milk from dairy cows using sniffer dogs.

Fast and accurate identification of disease-causing pathogens is essential for specific antimicrobial therapy in human and veterinary medicine. In these experiments, dogs were trained to identify Staphylococcus aureus and differentiate it from other common mastitis-causing pathogens by smell. Headspaces from agar plates, inoculated raw milk samples, or field samples collected from cows with Staphylococcus aureus and other mastitis-causing pathogens were used for training and testing. The ability to learn the specific odor of Staphylococcus aureus in milk depended on the concentration of the pathogens in the training samples. Sensitivity and specificity for identifying Staphylococcus aureus were 91.3 and 97.9%, respectively, for pathogens grown on agar plates; 83.8 and 98.0% for pathogens inoculated in raw milk; and 59.0 and 93.2% for milk samples from mastitic cows. The results of these experiments underline the potential of odor detection as a diagnostic tool for pathogen diagnosis.

Predicting stage 2 of calving in Holstein-Friesian heifers.

The objective of this study was to predict stage 2 of calving in Holstein-Friesian heifers. Interobserver reliability and predictive values of relevant signs of imminent parturition (i.e., tail raising, stepping, clear and bloody vaginal discharge, turning the head toward the abdomen, and lying lateral with abdominal contractions) were determined. In the first experiment 32 heifers were included. Three investigators participated as observers in the study. They walked through the precalving pen in pairs and observed pregnant heifers (≥267 d pregnant). Cohen's kappa results for the interobserver reliability were between 0.51 and 0.91. Thirty-seven Holstein-Friesian heifers were enrolled in the second experiment. Heifers were observed hourly for 24 h/d. Signs of imminent parturition that occurred were noted on a checklist. Compared with a precalving control period (4 d before calving), tail raising, clear vaginal discharge, and bloody vaginal discharge were more likely to occur during the last 24 h before calving. Two equations were built using the GENLINMIXED procedure to predict the hours until parturition. In version 1, the absence or presence of each sign of imminent parturition except turning the head toward the abdomen was included. In version 2, hours until parturition were estimated with the factors days of gestation, tail raising, and clear vaginal discharge. Relaxation of the broad pelvic ligaments and teat filling were evaluated twice per day. Prediction of calving with these parameters was not satisfying (positive predictive values were between 35.1 and 72.7% depending on the day of gestation). The possibility of excluding calving for the next 12 h was considerably higher, ranging from 88.5 to 97.1%. These results indicate that predicting stage 2 of calving via direct observation of plausible signs is imprecise and therefore not recommendable.

Udder firmness as a possible indicator for clinical mastitis.

Swelling of the mammary gland is an important sign to detect clinical mastitis (CM) in dairy cows. The overall objective of this study was to evaluate if udder firmness can be used as a cow-side indicator for mastitis and to evaluate how CM affects firmness within 14 d after diagnosis. A dynamometer was used to objectively determine udder firmness before and after milking in 45 cows with CM and 95 healthy cows. Udder firmness of both hind quarters was measured daily on 3 locations (upper, middle, lower measuring point) from the day of mastitis diagnosis until d 7 and again on d 14. Firmness of the middle measuring point was highest before and after milking in all cows. Udder firmness before milking was similar in quarters without and with CM. Subsequently, we concentrated on firmness measured on the middle point after milking. After milking, quarters with CM were firmer than healthy quarters. An increase of firmness of a quarter with mastitis did not affect firmness of the healthy neighboring quarter, nor did firmness of all healthy quarters differ. One firmness value per cow [i.e., Δ firmness (difference in udder firmness between both hind quarters)] was used for all further calculations. After fitting a generalized mixed model, CM affected Δ firmness in all cases. In multiparous cows, Δ firmness was also affected by continuous milk yield per day and DIM. Firmness thresholds for detection of CM were calculated using receiver operation characteristic curves. The threshold for detection of CM using Δ firmness was 0.282 kg (area under the curve: 0.722, sensitivity: 64.3%, specificity: 89.7%) and 0.425 kg (area under the curve: 0.817, sensitivity: 62.5%, specificity: 96.7%) in primiparous cows and multiparous cows, respectively. Linear mixed-model ANOVA were used to evaluate how CM affects udder firmness within 14 d after diagnosis. Cows with CM had a higher Δ firmness compared with cows without CM throughout the 14 d after the mastitis diagnoses. Parity had an effect on Δ firmness. Depending on systemic signs of sickness, mastitic cows were divided into cows having mild to moderate (n = 21) or severe mastitis (n = 24). Cows with severe mastitis suffered from a firmer udder on all measuring days. Bacteriological cure was defined based on 2 milk samples taken at 7 and 14 d after enrollment. An effect of parity and bacteriological cure on Δ firmness of cows with CM did not exist within the 14 d. Cows not clinically cured showed an increased Δ firmness of 0.560 kg compared with cured cows. In conclusion, udder firmness can be a useful indicator for CM. Further research is warranted to evaluate if udder firmness can be used as a predictor for the prognosis of a CM or the cure of inflammation.

Effect of Heat Stress on Concentrations of Faecal Cortisol Metabolites in Dairy Cows.

The negative impact of heat stress on health and productivity of dairy cows is well known. Heat stress can be quantified with the temperature-humidity index (THI) and is defined as a THI ≥ 72. Additionally, animal welfare is affected in cows living under heat stress conditions. Finding a way to quantify heat stress in dairy cows has been of increasing interest over the past decades. Therefore, the objective of this study was to evaluate concentrations of faecal glucocorticoid metabolites [i.e. 11,17-dioxoandrostanes (11,17-DOA)] as an indirect stress parameter in dairy cows without heat stress (DOA 0), with heat stress on a single day (acute heat stress, DOA 1) or with more than a single day of heat stress (chronic heat stress, DOA 2). Cows were housed in five farms under moderate European climates. Two statistical approaches (approach 1 and approach 2) were assessed. Using approach 1, concentrations of faecal 11,17-DOA were compared among DOA 0, DOA 1 and DOA 2 samples regardless of their origin (i.e. cow, unpaired comparison with a one-way anova). Using approach 2, a cow was considered as its own control; that is 11,17-DOA was treated as a cow-specific factor and only paired samples were included in the analysis for this approach (paired comparison with t-tests). In approach 1 (p = 0.006) and approach 2 (p = 0.038), 11,17-DOA values of cows under acute heat stress were higher compared to those of cows without heat stress. Our results also indicate that acute heat stress has to be considered as a confounder in studies measuring faecal glucocorticoid metabolites in cows to evaluate other stressful situations.

Validation of bovine oestrous-specific synthetic molecules with trained scent dogs; similarities between natural and synthetic oestrous smell.

Oestrous detection is crucial for successful dairy cow reproduction. Bulls identify cows in oestrus by oestrous-specific odours especially in urine and vaginal fluid. These have been used to train dogs to detect cows in heat. To improve and simplify the dog training, a spray containing synthetic oestrous molecules was developed. The objective of this study was to test the spray on similarities to the natural substance thus to assess its suitability as a training substance for heat detection dogs. Ten privately owned dogs of various breeds were trained. Dogs should be trained either to differentiate natural vaginal fluid from cows in oestrus and dioestrus (n = 5), or spray with or without synthetic oestrous molecules (n = 5). Dogs trained on natural fluid and on spray could detect the oestrous odour they had been trained on with an overall accuracy of 69.0% and 82.4%, respectively (p = 0.019). To validate the synthetic molecules, dogs trained with synthetic molecules had to detect oestrous odour in natural fluid without further training (accuracy 37.6%). Dogs trained on natural fluid detected the synthetic molecules with an accuracy of 50.0% (50% vs 37.4%, p < 0.05). Dogs can recognize natural vaginal fluid from cows in oestrus after they have been trained with synthetic oestrous molecules, but accuracy needs to be improved.

Behavioural and physiological assessment of stress reactions during vaginal examination in dairy cows.

OBJECTIVES AND AIM: The objective of this study was to determine the origin of an arched back in cows during vaginal examination. Moreover, we tested whether the duration of an arched back and avoidance reactions during vaginal examination can be decreased by epidural anaesthesia or analgesic treatment. MATERIAL AND METHODS: Behaviour during cleaning of the perivaginal region and during vaginal examination was scored using the avoidance reactivity score (ARS). Heart rate (HR) was recorded in 10 dairy cows considering four experimental phases, i.e. baseline, cleaning the perivaginal region, vaginal examination and post-examination. Each cow was examined three times and received no treatment (CON), an epidural anaesthesia (EPID) or an analgesic treatment (NSAID). The duration of an arched back during and post-examination was measured. RESULTS: The expression of the arched back was shortest in cows of group EPID and longest in cows of group CON. Avoidance reactions did not differ between the cleaning phase and vaginal examination in cows of group EPID. Cows of group CON showed the strongest avoidance reactions during examination, whereas cows of group EPID showed least avoidance reactions. Mean HR increased during cleaning and vaginal examination and decreased post-examination. Mean HR during vaginal examination did not differ between treatment groups. CONCLUSION: The results show that cows express discomfort during vaginal examination with an increase in avoidance reactions and HR. Although epidural anaesthesia could reduce sensitivity in the perivaginal region, cows still felt the urge to empty the vagina from the examiner's hand and, thus, were arching their back. CLINICAL RELEVANCE: In practice, routine vaginal examinations in dairy cows have not been considered as invasive examina- tions. Our results show that vaginal examinations indeed do cause discomfort. We do not suggest the application of any anaesthetic treatment as appropriate before routine vaginal examinations. Nonetheless, the examiner should be aware of the stress potential of vaginal examinations and conduct such examinations most carefully.

Evaluation of udder firmness by palpation and a dynamometer.

Swelling of the mammary gland is an important health status sign for clinical exploration and palpation is a routine diagnostic tool for mastitis detection in dairy cows. Data on repeatability or validity of specific methods of udder palpation are rare. The overall objective was to study the validity of estimates of udder firmness generated by palpation and by using a validated dynamometer. Specifically, we set out to determine within-observer repeatability and between-observer repeatability in 2 specific experiments. Additionally, we compared a 4-point palpation scoring system with estimates obtained with a dynamometer in this study. In a pilot trial, we determined the range of udder firmness of 25 cows and developed an in vitro model for udder firmness. This model enabled training of the observers and allowed investigating a 4-point palpation scoring system. In vivo, udder firmness was determined before and after milking by palpation and by using a dynamometer. Within-observer repeatability based on estimates of udder firmness of 25 cows obtained by 3 observers on a single day by palpation was 0.968. Within-observer repeatability of estimates of udder firmness of 25 cows obtained with the dynamometer by a single observer was 0.997. The coefficient of variation of the same measures was 9.1%. To determine between-observer repeatability (palpation: 0.932; dynamometer: 0.898), udder firmness of 100 cows was measured on 4 different days by 9 observers in experiment 2. Udder firmness in dairy cows could be measured repeatably with the dynamometer and by palpation, especially when performed by a single observer. Estimates of udder firmness generated by palpation and with the dynamometer were moderately related (correlation coefficient = 0.54). Training of observers through the pilot trial or practical experience in the 4 d of the study in experiment 2 did not improve the correlation. Further research is warranted to understand how udder firmness develops in infected udders.

Measurement of fecal glucocorticoid metabolites and evaluation of udder characteristics to estimate stress after sudden dry-off in dairy cows with different milk yields.

Sudden dry-off is an established management practice in the dairy industry. But milk yield has been increasing continuously during the last decades. There is no information whether the dry-off procedure, which often results in swollen and firm udders, causes stress, particularly in high-producing dairy cows. Therefore, we evaluated the effect of a sudden dry-off on extramammary udder pressure and the concentration of fecal glucocorticoid metabolites (i.e., 11,17-dioxoandrostane, 11,17-DOA) as an indirect stress parameter. Measurements were carried out within the last week before dry-off and until 9d after dry-off considering 3 groups of milk yield (i.e., low: <15 kg/d, medium: 15-20 kg/d, and high: >20 kg/d). Udder pressure increased in all yield groups after dry-off, peaked at d 2 after dry-off and decreased afterwards. Pressures were highest in high-yielding cows and lowest in low-yielding cows. But only in high-yielding cows was udder pressure after dry-off higher than before dry-off. Baseline 11,17-DOA concentrations depended on milk yield. They were highest in low-yielding (121.7 ± 33.3 ng/g) and lowest in high-yielding cows (71.1 ± 30.0 ng/g). After dry-off, 11,17-DOA increased in all yield groups and peaked at d 3. Whereas in medium- and high-yielding cows 11,17-DOA levels differed significantly from their respective baseline during the whole 9-d measuring period, low-yielding cows showed elevated 11,17-DOA levels only on d 3 after dry-off. However, especially the increase in 11,17-DOA after dry-off between the 3 yield groups was considerably different. Mean 11,17-DOA increase from baseline to d 3 was highest in high-yielding cows (129.1%) and considerably lower in low-yielding cows (40.1%). The highest fecal 11,17-DOA concentrations were measured on d 3 after dry-off, indicating that the stress was most intense on d 2, which is due to an 18-h time lag; at about the same time, udder pressure peaked. Our results showed a negligible effect of a sudden dry-off on low-yielding cows. High-yielding cows, however, faced high extramammary pressures and increased glucocorticoid production. Considering animal welfare aspects, a review of the current dry-off strategies might be warranted.

Short communication: Ability of dogs to detect cows in estrus from sniffing saliva samples.

Efficient estrus detection in high-producing dairy cows is a permanent challenge for successful reproductive performance. In former studies, dogs have been trained to identify estrus-specific odor in vaginal fluid, milk, urine, and blood samples under laboratory conditions with an accuracy of more than 80%. For on-farm utilization of estrus-detection dogs it would be beneficial in terms of hygiene and safety if dogs could identify cows from the feed alley. The objective of this proof of concept study was to test if dogs can be trained to detect estrus-specific scent in saliva of cows. Saliva samples were collected from cows in estrus and diestrus. Thirteen dogs of various breeds and both sexes were trained in this study. Five dogs had no experience in scent detection, whereas 8 dogs had been formerly trained for detection of narcotics or cancer. In the training and test situation, dogs had to detect 1 positive out of 4 samples. Dog training was based on positive reinforcement and dogs were rewarded with a clicker and food for indicating saliva samples of cows in estrus. A false indication was ignored and documented in the test situation. Dogs with and without prior training were trained for 1 and 5 d, respectively. For determining the accuracy of detection, the position of the positive sample was unknown to the dog handler, to avoid hidden cues to the dog. The overall percentage of correct positive indications was 57.6% (175/304), with a range from 40 (1 dog) to 75% (3 dogs). To our knowledge, this is the first indication that dogs are able to detect estrus-specific scent in saliva of cows.

Technical note: validating a dynamometer for noninvasive measuring of udder firmness in dairy cows.

Most measurements of udder pressure are based on devices connected to the gland cistern via cannulas. These devices are either inserted in the teat canal or surgically implanted into the udder tissue. In this study, instead of invasively measuring intramammary udder pressure, we measured the udder firmness noninvasively on the udder surface via a dynamometer. These are commonly used in food research to determine crispiness and firmness of fruits. The objective of this study was to validate a hand-held dynamometer for measuring udder firmness in dairy cows. Specifically we set out to determine inter-investigator repeatability considering potential confounders such as investigator, location, and cow. Through modifications in the standard operating procedure for the measurements, inter-investigator repeatability increased from correlation coefficient=0.80 (n=275) to correlation coefficient=0.94 (n=634). Measurements in different locations within the left hind quarter revealed a firmness gradient from the upper to the lower measuring point. Measurements between the 4 quarters within a cow displayed differences, except between both hind quarters. In 94.8% of the udders, firmness decreased due to milking. The correlation coefficient, however, between firmness changes and milk yield was low (r=0.42, n=153). Our data provide evidence that the dynamometer, although imperfect, does provide a reasonable measure of udder firmness and can be a useful tool in research related to animal health and welfare. However, a standardized operating protocol should be followed to minimize confounding by investigator, location, and quarter.

Short communication: Evaluation of vaginal discharge following treatment with a progesterone insert.

Yellowish discharge after application of intravaginal progesterone releasing inserts is frequently observed in cows. The objective of this study was to compare the bacteriological contamination of the vagina and uterus before and after a treatment with a progesterone insert in heifers. Forty-two Holstein heifers received a progesterone releasing insert [Eazi-Breed controlled internal drug release (CIDR) insert; Pfizer Animal Health, Berlin, Germany] for 7d. The protruding tail had been removed from half of the inserts (no tail group: n=21; tail group: n=21). Nine heifers from the tail group lost the insert within the 7-d treatment interval and were excluded. Heifers identified in estrus were artificially inseminated on d 9 or 10. Vaginal discharge was scored on a 4-point scale [vaginal discharge score (VDS) 0 to 3] and vaginal swabs were taken for bacteriological examination on d 0 and 7 and the day of artificial insemination (AI). Furthermore, cytological and bacteriological samples were obtained from the uterus on d 7 and the day of AI. On d 0, coliforms and Streptococcus spp. were found in vaginal swabs of 21 heifers (64%). On d 7, all heifers showed purulent vaginal discharge (VDS 2 to 3). The VDS was higher in the tail group compared with the no tail group. Arcanobacterium pyogenes, coliforms, and Streptococcus spp. were isolated from the vaginal swabs in 32 of 33 (96%) heifers on d 7. On the day of AI, VDS had improved to 0 or 1 in 96% of the heifers. However, A. pyogenes, coliforms, and Streptococcus spp. were still isolated in 17 of 33 (53%) heifers from the vagina and in 32 of 33 (96%) heifers from the endometrium. Endometrial cytology revealed polymorphonuclear neutrophils (PMN) in 11 heifers (6 to 32% PMN). Five samples exceeded the threshold of 5% PMN, and 2 samples exceeded the 10% PMN threshold, indicative of subclinical endometritis. In conclusion, pyogenic bacteria were found in the vagina and uterus on d 7 and the day of AI after intravaginal progesterone treatment. The severity of the discharge was affected by the protruding tail of the insert.

[Efficacy of vaginal progesterone inserts (CIDR) to reduce early embryonic losses in dairy cows]. / Wirksamkeit einer vaginalen Progesteronspange zur Verringerung embryonaler Verluste in der Frühträchtigkeit bei Milchkühen.

UNLABELLED: The OBJECTIVE of this study was to test the efficacy of a progesterone releasing device (CIDR®, Pfizer, Germany) inserted on day (d) 20 post insemination (p.i.) to reduce embryonic losses between d 27 and 39 p.i. Furthermore, we hypothesized that CIDR® increases blood progesterone levels during the application period, but does not affect the maintenance of pregnancy after removal. MATERIAL AND METHODS: The study was conducted on a commercial dairy farm, randomly allocating 74 Holstein Friesian cows to one of two groups. These cows were non-pregnant after previous artificial insemination and treated with an Ovsynch protocol. Group 1 (n=36) received a CIDR® on d 20 p.i. (CIDR® group) while group 2 (n=38) remained untreated (control group). CIDR® was removed on d 39 p.i. Blood samples were drawn from all cows on d 20, 27, 39 and 55 p.i. for analysis of progesterone (P4) concentrations by immunoassay (ADVIA Centaur®, Siemens, Germany). Pregnancy diagnosis was conducted on d 27 p.i. with ultrasonography, and on d 39 and 55 p.i. by transrectal palpation. RESULTS: The overall prevalence rate of early embryonic losses between d 27 and 39 p.i. was 37.1% (CIDR® group 35.3%, control group 38.9%; p=0.83). On d 39 p.i. 30.5% of all cows were pregnant and the percentage of pregnant cows did not differ between the study groups (p=0.83). Progesterone levels on d 20 p.i. did not differ among cows which were pregnant on d 39 p.i. (p=0.57). On d 27 p.i. progesterone levels in pregnant cows were higher in the CIDR® group (16.2 ± 9.9 ng/ml) compared to the control group (11.2 ± 3.4 ng/ml; p=0.04). Progesterone concentrations were lower on d 39 p.i., but still differed between study groups (p=0.05). After removal of CIDR®, blood progesterone levels did not differ between pregnant cows of both study groups on d 55 p.i. (p=0.36). CONCLUSION: The application of a progesterone releasing device led to increased blood progesterone levels during the application period, but did not affect maintenance of pregnancy after its removal. CLINICAL RELEVANCE: Supplementation with progesterone at d 20 p.i. does not decrease early embryonic losses between d 27 and 39 p.i. or increase the number of cows pregnant on d 39 p.i.

[Inappropriate lactation syndrome in goats--case collection and experiences with mastectomy]. / Lactatio falsa bei der Ziege - Fallsammlung und Erfahrungen mit der Amputation des Gesäuges.

OBJECTIVE: Hobby keeping of goats and sheep confronts veterinarians with new challenges that rarely have to be faced in livestock husbandry. During the last five years five goats were presented to the Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Germany, with inappropriate lactation syndrome. Four of these animals had been previously treated with cabergoline without enduring success. According to the request of the owners (informed consent) and the clinical severity of the cases, a mastectomy was performed in all five animals. MATERIAL AND METHODS: Surgery was performed under general anaesthesia using ketamine and xylazine, and with the patients in a recumbent position. RESULTS: Mastectomy in small ruminants requires knowledge of the anatomy of the udder and the possible positions of the supplying blood vessels. Our patients displayed a variety of dispositions of the Vena epigastrica caudalis superficialis. Special attention should be paid to a careful and blunt dissection of the mammary gland, and immediate control of haemorrhage, to maintain a clear view on the anatomic structures. Furthermore, dissection of the glandular tissue should be strictly avoided to prevent milk contamination of the surgical area. A sufficient skin flap has to be left to cover the surgical area after removal of the udder. CONCLUSION AND CLINICAL RELEVANCE: Even though udder amputation appears to be a radical and high-risk procedure, all five goats survived the surgery. The wound healing occurred in a reasonable time without any severe complications. In goats that are kept as "hobby animals" and in which an inappropriate lactation syndrome cannot be treated conservatively, mastectomy is a reasonable and promising therapy.

Efficacy of a treatment with hCG 4 days after AI to reduce pregnancy losses in lactating dairy cows after synchronized ovulation.

Content The objective of the study was to investigate whether a treatment with hCG 4 days after AI could reduce pregnancy losses in lactating dairy cows. Cows of a dairy herd presented to the veterinarian in a fixed reproductive management protocol were treated with an Ovsynch protocol if no corpus luteum (CL) could be palpated per rectum (Group OV). Cows with a CL received cloprostenol (0.15 mg). After 2 days, these cows were treated with buserelin (0.01 mg) and received timed AI 16-20 h later (Group PG). In both treatment protocols, cows were assigned to two groups to receive 2500 IU of hCG i.v. 4 days after AI or to serve as untreated controls (Groups OV-hCG, OV-Control, PG-hCG and PG-Control). Pregnancy diagnosis was carried out 27 days after AI via ultrasonography and 39 days after AI by rectal palpation. Pregnancy losses were defined as cows being pregnant on day 27 but not pregnant on day 39 after AI. Pregnancy rate (PR) by day 27 did not differ among the four groups (35.4, 35.0, 37.0 and 38.0% for Groups OV-hCG, OV-Control, PG-hCG and PG-Control, respectively). Pregnancy losses between day 27 and day 39 after AI were smaller in hCG treated animals in summer but not in autumn and spring. Pregnancy rate by day 39 after AI was higher in PG than in OV groups, but independent of hCG-treatment. In conclusion, treatment with hCG 4 days after AI did not significantly increase PR on 39 days after AI. A positive effect of hCG on pregnancy losses during the summer months warrants further investigation.

Serosurvey for selected infectious disease agents in free-ranging black and white rhinoceros in Africa.

Two hundred and eighty one serum samples collected from free-ranging black (Diceros bicornis) and white (Ceratotherium simum) rhinoceros, in the Republic of South Africa (RSA), Namibia, and Kenya from 1987-97, were examined for antibody to 16 different infectious agents. Positive antibody titers were detected against Akabane (59.8%), bluetongue (55%), African horse sickness (27.9%), epizootic haemorrhagic disease of deer (19.4%), parainfluenza type 3 (25.3%), bovine herpes virus 1 (3.1%), equine herpes virus 1 (8.8%) and bovine viral diarrhea (1.2%) viruses, and four serovars of Leptospira interrogans, (ranging 1.2 to 8.8%). No antibody was detected against Rift Valley fever virus, encephalomyocarditis virus, Brucella abortus, and Trypanosoma equiperdum. Interspecies differences were detected for African horse sickness, epizootic haemorrhagic disease of deer and parainfluenza type 3 viruses. There appeared to be some geographic variation in the prevalence of antibody for African horse sickness, bluetongue, epizootic haemorrhagic disease of deer, parainfluenza type 3, equine herpes virus 1 and Leptospira interrogans serovar bratislava.