ABSTRACT
O objetivo deste estudo foi investigar a prevalncia e a severidade da fluorose dent ria em crianas de uma escola municipal de Passo Fundo - RS. A amostra foi composta por 121 escolares de 9 a 12 anos da Escola Municipal de Ensino Fundamental Guaracy Barroso Marinho. A coleta de dados foi realizada no ms de agosto de 2011 e o instrumento utilizado foi o exame cl¡nico intrabucal, seguindo as determinaães do ¡ndice de Dean10. Foram realizadas an lises descritivas das vari veis e os resultados mostraram uma prevalncia de fluorose dent ria de 28,9%, onde o grau de severidade mais frequente foi o muito leve com 23,1%. Concluiu-se que a prevalncia de fluorose dent ria nos escolares foi elevada, porm, com baixa severidade
The objective of this study was to assess the prevalence and severity of dental fluorosis in students from a public school located at Passo Fundo (RS- Brazil). The sample consisted of 121 schoolchildren, age between 9 and 11 years, from the Escola Municipal de Ensino Fundamental Guaracy Barroso Marinho. Data were collected in August 2011 through clinical examination and in accordance to Deans fluorosis index10. Descriptive data analysis was performed and the results indicated a prevalence of dental fluorosis of 28.9%. In regard to the severity of the fluorosis the most frequent was very mild fluorosis (23.1%). It was observed that there is a high prevalence of dental fluorosis among the students, but mostly of low severity
Subject(s)
Child , Diagnosis, Oral , Fluorosis, Dental , Health Surveys/methods , Prevalence , Oral Health/education , Data Interpretation, StatisticalABSTRACT
BACKGROUND: Colloidal silver has been used as an antimicrobial and disinfectant agent. However, there is scarce information on its antitumor potential. The aim of this study was to determine if colloidal silver had cytotoxic effects on MCF-7 breast cancer cells and its mechanism of cell death. METHODS: MCF-7 breast cancer cells were treated with colloidal silver (ranged from 1.75 to 17.5 ng/mL) for 5 h at 37°C and 5% CO2 atmosphere. Cell Viability was evaluated by trypan blue exclusion method and the mechanism of cell death through detection of mono-oligonucleosomes using an ELISA kit and TUNEL assay. The production of NO, LDH, and Gpx, SOD, CAT, and Total antioxidant activities were evaluated by colorimetric assays. RESULTS: Colloidal silver had dose-dependent cytotoxic effect in MCF-7 breast cancer cells through induction of apoptosis, shown an LD50 (3.5 ng/mL) and LD100 (14 ng/mL) (*P < 0.05), significantly decreased LDH (*P < 0.05) and significantly increased SOD (*P < 0.05) activities. However, the NO production, and Gpx, CAT, and Total antioxidant activities were not affected in MCF-7 breast cancer cells. PBMC were not altered by colloidal silver. CONCLUSIONS: The present results showed that colloidal silver might be a potential alternative agent for human breast cancer therapy.
