ABSTRACT
Changes in the activities of NAD+- and NADP-dependent dehydrogenases of lymphocytes and tumor cells were studied in mice with Ehrlich ascites carcinoma, as well as changes in the concentrations of oxaloacetate, lactate, and NAD+ in the course of tumor growth. During the major period of tumor growth, conditions are produced in the lymphocytes for increased intensity of aerobic reactions directed at energy reproduction combined with a somewhat decreased intensity of synthetic processes. In the tumor cells, conditions predominantly arise for intensification of plastic metabolic reactions and reactions related to anaerobic energy reproduction.
Subject(s)
Carcinoma, Ehrlich Tumor/enzymology , Energy Metabolism , Lymphocytes/enzymology , Oxidoreductases/metabolism , Aerobiosis , Anaerobiosis , Animals , Carcinoma, Ehrlich Tumor/pathology , Female , Lactic Acid/metabolism , Lymphocytes/pathology , Male , Mice , Mice, Inbred ICR , NAD/metabolism , NADP/metabolism , Oxaloacetic Acid/metabolismABSTRACT
We have developed a new bioluminescent method for determination of concentrations of pyruvate, lactate, malate, glutamate and NAD+ in the periferal blood lymphocytes from mice with Ehrlich ascites carcinoma. There were blood samples of intact mice for control. We found that variations of these substrates, depend on stage of malignant growth.