The Emerging Role of 3-Hydroxyanthranilic Acid on C. elegans Aging Immune Function.

3-hydroxyanthranilic acid (3HAA) is considered to be a fleeting metabolic intermediate along tryptophan catabolism through the kynurenine pathway. 3HAA and the rest of the kynurenine pathway have been linked to immune response in mammals yet whether it is detrimental or advantageous is a point of contention. Recently we have shown that accumulation of this metabolite, either through supplementation or prevention of its degradation, extends healthy lifespan in C. elegans and mice, while the mechanism remained unknown. Utilizing C. elegans as a model we investigate how 3HAA and haao-1 inhibition impact the host and the potential pathogens. What we find is that 3HAA improves host immune function with aging and serves as an antimicrobial against gram-negative bacteria. Regulation of 3HAA's antimicrobial activity is accomplished via tissue separation. 3HAA is synthesized in the C. elegans hypodermal tissue, localized to the site of pathogen interaction within the gut granules, and degraded in the neuronal cells. This tissue separation creates a new possible function for 3HAA that may give insight to a larger evolutionarily conserved function within the immune response.

Convolutional neural network misclassification analysis in oral lesions: an error evaluation criterion by image characteristics.

OBJECTIVE: This retrospective study analyzed the errors generated by a convolutional neural network (CNN) when performing automated classification of oral lesions according to their clinical characteristics, seeking to identify patterns in systemic errors in the intermediate layers of the CNN. STUDY DESIGN: A cross-sectional analysis nested in a previous trial in which automated classification by a CNN model of elementary lesions from clinical images of oral lesions was performed. The resulting CNN classification errors formed the dataset for this study. A total of 116 real outputs were identified that diverged from the estimated outputs, representing 7.6% of the total images analyzed by the CNN. RESULTS: The discrepancies between the real and estimated outputs were associated with problems relating to image sharpness, resolution, and focus; human errors; and the impact of data augmentation. CONCLUSIONS: From qualitative analysis of errors in the process of automated classification of clinical images, it was possible to confirm the impact of image quality, as well as identify the strong impact of the data augmentation process. Knowledge of the factors that models evaluate to make decisions can increase confidence in the high classification potential of CNNs.

Proximal jejunal intussusception due to clear cell renal carcinoma metastasis.

The incidence of metastasis from clear cell renal carcinoma (CCR) to the small intestine is relatively rare. While most patients exhibit symptoms such as upper gastrointestinal bleeding or obstructive symptoms, the identification of small intestine metastases often faces diagnostic delays.

On the benefits of the tryptophan metabolite 3-hydroxyanthranilic acid in Caenorhabditis elegans and mouse aging.

Tryptophan metabolism through the kynurenine pathway influences molecular processes critical to healthy aging including immune signaling, redox homeostasis, and energy production. Aberrant kynurenine metabolism occurs during normal aging and is implicated in many age-associated pathologies including chronic inflammation, atherosclerosis, neurodegeneration, and cancer. We and others previously identified three kynurenine pathway genes-tdo-2, kynu-1, and acsd-1-for which decreasing expression extends lifespan in invertebrates. Here we report that knockdown of haao-1, a fourth gene encoding the enzyme 3-hydroxyanthranilic acid (3HAA) dioxygenase (HAAO), extends lifespan by ~30% and delays age-associated health decline in Caenorhabditis elegans. Lifespan extension is mediated by increased physiological levels of the HAAO substrate 3HAA. 3HAA increases oxidative stress resistance and activates the Nrf2/SKN-1 oxidative stress response. In pilot studies, female Haao knockout mice or aging wild type male mice fed 3HAA supplemented diet were also long-lived. HAAO and 3HAA represent potential therapeutic targets for aging and age-associated disease.

Use of Artificial Intelligence in the Classification of Elementary Oral Lesions from Clinical Images.

OBJECTIVES: Artificial intelligence has generated a significant impact in the health field. The aim of this study was to perform the training and validation of a convolutional neural network (CNN)-based model to automatically classify six clinical representation categories of oral lesion images. METHOD: The CNN model was developed with the objective of automatically classifying the images into six categories of elementary lesions: (1) papule/nodule; (2) macule/spot; (3) vesicle/bullous; (4) erosion; (5) ulcer and (6) plaque. We selected four architectures and using our dataset we decided to test the following architectures: ResNet-50, VGG16, InceptionV3 and Xception. We used the confusion matrix as the main metric for the CNN evaluation and discussion. RESULTS: A total of 5069 images of oral mucosa lesions were used. The oral elementary lesions classification reached the best result using an architecture based on InceptionV3. After hyperparameter optimization, we reached more than 71% correct predictions in all six lesion classes. The classification achieved an average accuracy of 95.09% in our dataset. CONCLUSIONS: We reported the development of an artificial intelligence model for the automated classification of elementary lesions from oral clinical images, achieving satisfactory performance. Future directions include the study of including trained layers to establish patterns of characteristics that determine benign, potentially malignant and malignant lesions.

Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices.

The nematode Caenorhabditis elegans is among the most common model systems used in aging research owing to its simple and inexpensive culture techniques, rapid reproduction cycle (~3 days), short lifespan (~3 weeks), and numerous available tools for genetic manipulation and molecular analysis. The most common approach for conducting aging studies in C. elegans, including survival analysis, involves culturing populations of tens to hundreds of animals together on solid nematode growth media (NGM) in Petri plates. While this approach gathers data on a population of animals, most protocols do not track individual animals over time. Presented here is an optimized protocol for the long-term culturing of individual animals on microfabricated polydimethylsiloxane (PDMS) devices called WorMotels. Each device allows up to 240 animals to be cultured in small wells containing NGM, with each well isolated by a copper sulfate-containing moat that prevents the animals from fleeing. Building on the original WorMotel description, this paper provides a detailed protocol for molding, preparing, and populating each device, with descriptions of common technical complications and advice for troubleshooting. Within this protocol are techniques for the consistent loading of small-volume NGM, the consistent drying of both the NGM and bacterial food, options for delivering pharmacological interventions, instructions for and practical limitations to reusing PDMS devices, and tips for minimizing desiccation, even in low-humidity environments. This technique allows the longitudinal monitoring of various physiological parameters, including stimulated activity, unstimulated activity, body size, movement geometry, healthspan, and survival, in an environment similar to the standard technique for group culture on solid media in Petri plates. This method is compatible with high-throughput data collection when used in conjunction with automated microscopy and analysis software. Finally, the limitations of this technique are discussed, as well as a comparison of this approach to a recently developed method that uses microtrays to culture isolated nematodes on solid media.

Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions.

Caenorhabditis elegans are widely used to study aging biology. The standard practice in C. elegans aging studies is to culture groups of worms on solid nematode growth media (NGM), allowing the efficient collection of population-level data for survival and other physiological phenotypes, and periodic sampling of subpopulations for fluorescent biomarker quantification. Limitations to this approach are the inability to (1) follow individual worms over time to develop age trajectories for phenotypes of interest and (2) monitor fluorescent biomarkers directly in the context of the culture environment. Alternative culture approaches use liquid culture or microfluidics to monitor individual animals over time, in some cases including fluorescence quantification, with the tradeoff that the culture environment is contextually distinct from solid NGM. The WorMotel is a previously described microfabricated multi-well device for culturing isolated worms on solid NGM. Each worm is maintained in a well containing solid NGM surrounded by a moat filled with copper sulfate, a contact repellent for C. elegans, allowing longitudinal monitoring of individual animals. We find copper sulfate insufficient to prevent worms from fleeing when subjected to aversive interventions common in aging research, including dietary restriction, pathogenic bacteria, and chemical agents that induce cellular stress. The multi-well devices are also molded from polydimethylsiloxane, which produces high background artifacts in fluorescence imaging. This protocol describes a new approach for culturing isolated roundworms on solid NGM using commercially available polystyrene microtrays, originally designed for human leukocyte antigen (HLA) typing, allowing the measurement of survival, physiological phenotypes, and fluorescence across the lifespan. A palmitic acid barrier prevents worms from fleeing, even in the presence of aversive conditions. Each plate can culture up to 96 animals and easily adapts to a variety of conditions, including dietary restriction, RNAi, and chemical additives, and is compatible with automated systems for collecting lifespan and activity data.

Efficacy of a Fungal Formulation with the Nematophagous Fungus Pochonia chlamydosporia in the Biological Control of Bovine Nematodiosis.

In the control of bovine worms, biological control by nematophagous fungi stands out, especially Pochoniachlamydosporia which causes the destruction of helminth eggs. This study aims to test the effectiveness of a formulation containing the nematophagous fungus Pochonia chlamydosporia isolated for the biological control of bovine nematodiosis. Twelve cattle were divided into two groups: control group (GC) and the group that received the formulation (GT). Feces and pasture samples were collected for the research of gastrointestinal nematodes. Lung worms and trematodes were investigated. The animals were weighed monthly. The averages of temperature and rainfall were recorded. The supply of the fungus Pochonia chlamydosporia was not effective in reducing the eggs per gram of feces of gastrointestinal nematodes (EPG) of the animals, not differing statistically (p > 0.05) between the groups. The mean values of larvae recovered in the pasture did not differ significantly (p > 0.05). The genus Haemonchus sp. was the most prevalent. There was no correlation between the number of larvae with temperature and rainfall (p > 0.05). There was a statistically significant difference (p < 0.05) in the penultimate weighing of the experiment. The formulation containing Pochonia chlamydosporia was not efficient in the biological control of bovine gastrointestinal nematodes.

Serine carboxypeptidases from the carnivorous plant Nepenthes mirabilis: Partial characterization and heterologous expression.

This study aimed to partially characterize the three main serine carboxypeptidases (SCP3, SCP20, and SCP47) from Nepenthes mirabilis. Furthermore, one peptidase (SCP3) was chosen for further heterologous expression in Escherichia coli Shuffle®T7. SCP3 also was characterized in terms of its allergenic potential using bioinformatics tools. SCP3, SCP20, and SCP47 showed very similar 3D structures and mechanistic features to other plant serine peptidases belonging to clan SC and family S10. Although SCP3 was obtained in its soluble form, using 1% ethanol during induction with 0.5 mM IPTG at 16 °C for 18 h, it did not show proteolytic activity by zymography or in vitro analysis. SCP3 presented a few allergenic peptides and several cleavage sites for digestive enzymes. This work describes additional features of these enzymes, opening new perspectives for further studies for characterization and analysis of heterologous expression, as well as their potential biotechnological applications.

Functional Coronary Artery Assessment: a Systematic Literature Review.

Cardiovascular diseases represent the number one cause of death in the world, including the most common disorders in the heart's health, namely coronary artery disease (CAD). CAD is mainly caused by fat accumulated in the arteries' internal walls, creating an atherosclerotic plaque that impacts the blood flow functional behavior. Anatomical plaque characteristics are essential but not sufficient for a complete functional assessment of CAD. In fact, plaque analysis and visual inspection alone have proven insufficient to determine the lesion severity and hemodynamic repercussion. Furthermore, the fractional flow reserve (FFR) exam, which is considered the gold standard for stenosis functional impair determination, is invasive and contains several limitations. Such a panorama evidences the need for new techniques applied to image exams to improve CAD functional assessment. In this article, we perform a systematic literature review on emerging methods determining CAD significance, thus delivering a unique base for comparing these methods, qualitatively and quantitatively. Our goal is to guide further studies with evidence from the most promising methods, highlighting the benefits from both areas. We summarize benchmarks, metrics for evaluation, and challenges already faced, thus shedding light on the requirements for a valid, meaningful, and accepted technique for functional assessment evaluation. We create a base of comparison based on quantitative and qualitative indicators and highlight the most relevant geometrical metrics that correlate with lesion significance. Finally, we point out future benchmarks based on recent literature.

Formulation of the nematophagous fungus Duddingtonia flagrans in the control of equine gastrointestinal parasitic nematodes.

Equine gastrointestinal nematodiosis contributes to the lower productivity of these animals. There are growing reports of the emergence of nematodes resistant to the drugs used for decades in anthelmintic treatments. An alternative to the emergence of resistance may be the use of nematophagous fungi as a complementary method of treatment. Therefore, the objective was to evaluate the effects of the use of the product Bioverm® as a carrier of Duddingtonia flagrans, on pasture contamination level and equine parasitic burden. Sixteen mares were used, divided into two groups, one control and one treated, in which the treated animals received a dose of 1 g of Bioverm®, containing 105 chlamydospores per gram of the commercial product for each 10 kg of body weight, per day, for six months. Efficacy was evaluated by count of eggs per gram of faeces (EPG), coproculture and larval count on pastures and its correlation with climate, as well as weight gain evaluation. During the study, a significant influence of the formulation on weight gain and EPG was observed. The recovery of larvae from coprocultures revealed the predominance of small over large strongyles. There was a significant difference (p < 0.05) between the averages of the number of larvae of small strongyles recovered in the pasture at a distance of 0-20 cm from the faeces of treated and control groups. A correlation was also observed between the number of larvae recovered from the pasture and the average temperature during the experimental period, mainly in August and September. The ingestion of Bioverm® enhances the biological control of gastrointestinal nematodes of pasturing horses.

Arthrobotrys cladodes and Pochonia chlamydosporia in the biological control of nematodiosis in extensive bovine production system.

Cattle production in extensive systems favours the occurrence of gastrointestinal nematodes, and the use of nematophagous fungi complements the control strategies for these nematodes. The aim of this study was to evaluate the effectiveness of the fungi Arthrobotrys cladodes and Pochonia chlamydosporia in the biological control of gastrointestinal parasitic nematodes in grazing cattle. Twenty-four calves were randomly divided into four groups and allocated to independent paddocks from February 2018 to January 2019. In the first group, the animals received pellets containing P. chlamydosporia. In the second group, the animals received pellets containing A. cladodes. In the third group, the animals received pellets containing a combination of the fungi A. cladodes and P. chlamydosporia. In the control group, the animals received pellets without fungus. The combined use of A. cladodes and P. chlamydosporia showed greater efficacy in the biological control of bovine gastrointestinal parasitic nematodes than the same fungi used separately. The parasite load was lower and weight gain was greater (P ⩽ 0.05) in the groups of cattle treated with nematophagous fungi. Therefore, the use of A. cladodes and P. chlamydosporia is promising in the biological control of nematodiosis in cattle.

Genetic variants of VWF gene in type 2 von Willebrand disease.

INTRODUCTION: von Willebrand disease (VWD) is the most common inherited bleeding disorder. Few studies have explored the molecular basis of type 2 VWD. AIM: This study aimed to identify variants associated with type 2 VWD. METHODS: We collected clinical and laboratory data, as well as response to desmopressin and bleeding assessment tool (BAT) score in patients diagnosed with type 2 VWD. We sequenced exons 17, 18, 20 and 28 of the VWF gene. RESULTS: We identified 19 different variants in 40 unrelated patients (47.5%). Most of the variants (84.2%) were found in exon 28. A total of 10/19 variants (52.6%) were identified as "likely causative" in 17/40 patients (42.5%), according to the ISTH-SSC and EAHAD VWF gene mutations databases. Nine variants were initially identified as potentially benign. However, through analyses in silico, four of these variants were reclassified as "likely pathogenic" (Ile1380Val, Asn1435Ser, Ser1486Leu and Tyr1584Cys). Response to desmopressin was associated with three variants: Met740Ile, Arg1597Gln and Tyr1584Cys. Major bleeding was associated with variants related to VWD subtypes 2B and 2M. CONCLUSION: In conclusion, we identified 19 variants, of which 14 are "likely pathogenic" and therefore associated with VWD. We suggest a possible association of pathogenic variants with major bleeding, response to desmopressin and BAT score ≥10, although this requires further confirmation.

Silage from heart-of-palm waste produced from Alexander palm tree / Ensilaje de residuos de palmito producidos a partir de Palma Real / Silagem de resíduos de palmito produzidos a partir da Palmeira Real

Abstract Background: Production of heart-of-palm from Alexander palm tree generates a waste that could be used as silage for feeding ruminants. Objective: To evaluate the chemical composition, fermentation parameters, and quality of silage made from waste of heart-of-palm production using Alexander palm. Methods: Three types of silage made from waste of heart-of-palm production were evaluated: 1) leaf silage, 2) sheath silage, and 3) compound (leaf + sheath) silage. The processed waste was packed in experimental silos and distributed in a completely randomized design with 10 replications. Means were compared pairwise using Tukey's test at the 5% significance level. Results: Leaf silage had the highest pH (p<0.05) and the lowest ammonia nitrogen (p<0.05) in relation to the other silages. No difference was observed (p>0.05) for lactic and butyric acids, whereas acetic acid was higher (p<0.05) in the sheath and compound silages. Dry matter and crude protein contents were the highest (p<0.05) in leaf silage. Neutral detergent fiber was higher (p<0.05) in sheath silage, followed by leaf and compound silages, respectively. The highest lignin content (p<0.05) was observed in sheath silage. Conclusions: Based on the fermentative parameters, waste from heart-of-palm from Alexander palm tree has potential for silage production. However, differences in silage composition at the time of feeding ruminants should be considered.

Resumen Antecedentes: Los residuos de la producción de palmito de Palma Real podrian utilizarse como ensilaje para alimentación de rumiantes. Objetivo: Determinar la composición, parámetros químicos de fermentación, y calidad del ensilaje elaborado a partir de residuos procedentes de la producción de palmito de Palma Real. Métodos: Tres tipos de ensilaje hechos con los residuos generados a partir de la producción de palmito fueron evaluados: 1) ensilaje de hojas, 2) ensilaje de vainas, y 3) ensilaje compuesto (hojas + vainas). Los residuos tratados fueron empacados en silos experimentales y distribuidos en un diseño completamente al azar con 10 repeticiones. Las medias fueron comparadas mediante la prueba de Tukey, con un nivel de significancia del 5%. Resultados: El ensilaje de hojas mostró el pH más alto (p<0,05) y el nivel más bajo de nitrógeno amoniacal (p<0,05) en comparación con los otros ensilajes. No se observaron diferencias (p>0,05) en los ácidos láctico y butírico, mientras que el ácido acético fue mayor en el ensilaje de vainas y en el compuesto en relación con el otro ensilaje. La materia seca (MS) y el contenido de proteína cruda (PC) fueron mayores (p<0,05) en el ensilaje de hojas. Los contenidos de fibra detergente neutra fueron mayores (p<0,05) en los ensilajes de vainas, seguido por los ensilajes de hojas y compuesto, respectivamente. El contenido de lignina fue más alto (p<0,05) en el ensilaje de vainas. Conclusiones: Según los parámetros fermentativos, los residuos de la producción de palmito de Palma Real tienen potencial para la producción de ensilaje. Sin embargo, se deben considerar las diferencias en la composición de los ensilajes al momento de alimentar rumiantes.

Resumo Antecedentes: Resíduos da produção de palmito da palmeira Real são um problema para a agroindústria devido sua falta de utilização e o seu uso na forma de silagem pode ser uma alternativa na alimentação de ruminantes. Objetivo: Avaliar a composição química e os parâmetros fermentativos, e verificar a qualidade da silagem produzida a partir do resíduo da produção de palmito da Palmeira Real Australiana. Métodos: Foram avaliadas três tipos de silagem de resíduo da produção de palmito da Palmeira Real: silagem de folha, silagem de bainha e silagem da composta (folha + bainha). O resíduo processado foi acondicionado em silos experimentais e distribuídos em um delineamento inteiramente casualizado com 10 repetições e as médias comparadas pelo teste de Tukey a 5% de significância. Resultados: A silagem de folha apresentou maior pH (p<0,05) em relação as demais silagens, enquanto para o nitrogênio amoniacal (p<0,05), apresentou menor teor em relação as demais silagens. Não observou-se diferença (p>0,05) para os ácidos lático e butírico, enquanto o ácido acético foi maior para as silagens de bainha e composta em relação as demais silagens. O teor de matéria seca (MS), proteína bruta (PB) foi maior (p<0,05) na silagem de folha em relação as demais silagens. A fibra em detergente neutro analisada estimada apresentou maiores valores (p<0,05) para a silagem de bainha, seguida pela silagem de folhas e silagem composta, respectivamente. O teor de lignina foi maior (p<0,05) para a silagem de bainha. Conclusões: De acordo com os parâmetros fermentativos, os resíduos da produção de palmito da Palmeira Real apresentam potencial para a produção de silagem. No entanto, as diferenças na composição das silagens devem ser consideradas quando oferecidas para animais ruminantes.

Using the fungus Arthrobotrys cladodes var. macroides as a sustainable strategy to reduce numbers of infective larvae of bovine gastrointestinal parasitic nematodes.

Research in the area of sanitation in ruminant production has focused on discovery of potential agents for biological control of helminths with nematophagous fungi and has provided evidence of success. The antagonistic potential of the fungus Arthrobotrys cladodes var. macroides on infective larvae of bovine gastrointestinal parasitic nematodes was evaluated by scanning electron microscopy. Additionally, an in vivo test of the resistance to digestive processes and viability of the fungus was carried out using a formulation based on sodium alginate administered orally in cattle. Production of conidia and chlamydospores was high. In in vitro tests, the number of infective nematode larvae was reduced 68.7% by the fungus in the treated group compared to the control group. The interaction between the fungus and the nematodes was confirmed by scanning electron microscopy. Plates containing fecal samples collected after oral administration of 100 g of pellets containing the A. cladodes fungus showed that the fungus survived passage through the gastrointestinal tract of ruminants, grew on agar, formed traps and preyed on L3 larvae of gastrointestinal parasites. The results of the present study provide a new opportunity for alternative, environmentally safe control of ruminant nematodes.

Reduction of bovine strongilides in naturally contaminated pastures in the southeast region of Brazil.

Biological control through the use of nematophagous fungi is a sustainable alternative for combatting helminthes in domestic animals and allows a reduction in the use of anthelmintics. The objective of this research was to evaluate the efficacy of the Arthrobotrys cladodes var macroides fungus in a pelleted formulation, based on sodium alginate and administered twice a week orally, as an alternative for the biological control of nematodes in field-grown young cattle. The experiment was conducted in a farm located in the municipality of Viçosa, MG, where 12 cattle, seven to nine months old, were allocated in two groups (treated group and control group) and distributed in pickets of Brachiaria decumbens, naturally infested with nematode larvae. The animals in the treated group received 1g of sodium alginate matrix pellets for every 10 kg of animal live weight, containing the nematophagous fungus Arthrobotrys cladodes var macroides and administered twice a week in conjunction with commercial feed. In the control group, each animal received 1 g of pellets for every 10 kg of animal live weight, without fungal mycelium added to the feed. Samples of feces and pastures were collected fortnightly for 12 months. The results showed that the most prevalent nematode genera in the coprocultures were Haemonchus sp., Cooperia sp. and Oesophagostomum sp., reflecting the results found in forage. The pasture that contained the animals that received feed with the fungus presented a reduction of 59% and 52% of larvae recovered at distances of 20 cm and 40 cm from the fecal pats, respectively. The mean number of eggs per gram of feces each month and animal body weight did not differ (p > 0.05) between the treated and control groups. Stool and soil samples from both groups were colonized by A. cladodes fungus and other fungi. Administration of Arthrobotrys cladodes var macroides mycelium by means of a sodium alginate matrix twice weekly reduced larval infestation of the surrounding pasture, indicating that this fungus may be a promising biological control of infecting forms of nematodes present in the environment.

Duddingtonia flagrans formulated in rice bran in the control of Oesophagostomum spp. intestinal parasite of swine.

Three experimental assays with Duddingtonia flagrans (isolated AC001) were carried out. The growth of the genus Duddingtonia present in formulation of rice bran, its predatory capability on Oesophagostomum spp. infective larvae (L3) in petri dishes (assay 1), its action in faecal cultures with eggs of that parasite (assay 2) and isolate's capability of predation after passing through gastrointestinal tract of swine (assay 3) was evaluated. At assay 3, feces were collected at time intervals of 12, 24, 36, 48, and 60 h after feed animals with the formulation. Assays 1 and 2 showed a statistical difference (p < 0.01) by the F test when comparing the treated group with the control group. At the both assays, was observed in the treated group a reduction percentage of 74.18% and 88.38%, respectively. In assay 3, there was a statistical difference between the treated group and the control group at all collection times (p < 0.01). Regarding the collection periods, there was no statistical difference over time in the treatment group (p > 0.05). The results demonstrate that the fungal isolate AC001 formulated in rice bran can prey on L3 of Oesophagostomum spp., in vitro and after passing through the gastrointestinal tract, without loss of viability. This isolate may be an alternative in the control of Oesophagostomum spp. in swine.

Substituição do resíduo da produção de palmito da Palmeira Real Australiana (Archontophoenix alexandrae) na silagem de cana-de-açúcar em dietas de ovinos / Replacing of residue from production of palm Palm Royal Australian (Archontophoenix alexandrae) in silage of sugar cane in diets of sheep

Objetivou-se avaliar a composição química, consumo voluntário e digestibilidade aparente de dietas contendo resíduo oriundo da produção de palmito da palmeira Real Australiana (Archontophoenix alexandrae) em substituição à cana-de-açúcarem ovinos. Foram utilizados 12 ovinos com peso vivo médio de 23,3 ± 2,8 kg, mantidos em gaiolas metabólicas distribuídos em seis quadrados latinos 2x2 implementado em esquema fatorial 3 x 2 (três tipos de resíduo - folha, bainha e composta - e dois níveis de substituição do resíduo, 5% e 15%). Verificou-se maior consumo de matéria seca (MS), matéria orgânica (MO), carboidratos não fibrosos (CNF) na substituição pelo resíduo composta. Os valores médios de digestibilidade aparente da MS, MO, Proteína bruta (PB), fibra insolúvel em detergente neutro corrigido para cinzas e proteína(FDNcp) e nível dietético de NDT foram maiores para o resíduo folha. Constatou- se efeito de interação entre resíduo e o nível de substituição sobre as excreções urinárias de nitrogênio total (EUN), balanço nitrogenado aparente (BNA) e compostos nitrogenados microbianos (NMIC). Resíduos da produção de palmito oriundos da palmeira Real Australiana podem ser utilizados como alimentos volumosos na dieta de animais ruminantes, sendo destes, o resíduo folha e composta os que apresentaram melhores respostas nas características avaliadas.

The aim of this study was to evaluate the chemical composition, voluntary intake and apparent digestibility of the diets containing residue from palm heart of Australian Royal Palm (Archontophoenix alexandrae) to replace sugar cane on sheep. Twelve sheep were used with average live weight of 23,3 ± 2,8 Kg and they placed in metabolism cages and distributed in six latin square 2 x 2 in a factorial design 3 x 2 (three types of residue -sheet, bark and composed - and two levels of residue’s replacement, 5% and 15%). It was observed higher intake of dry matter (DM), organic matter (OM), nonfiber carbohydrates (NFC) by substitution of composed residue. The average values of apparent digestibility of DM, OM, crude protein (CP), neutral detergent fibre correct for ash and protein (NDFap) and total digestible nutrients (TDN) were higher for sheet residue. There was interaction between type of residue and level of residue’s replacement on the urinary excretion of total nitrogen (NUE), apparent nitrogen balance (BNA) and microbial nitrogen compost (NMIC). Residues from palm heart of Australian Royal Palm can be used as roughage in the ruminants’diet, and of these residues, the sheet and composed residue showed better response in the evaluated characteristics.