Uso da classificação de robson para avaliação das taxas de cesáreas em hospitais paranaenses vinculados ao sistema único de saúde / Use of the robson classification for the evaluation of cesarean section rates in hospitals in the state of paraná linked to the single health system / Uso de la clasificación de robson para evaluar las tasas de cesrea en hospitales paranaeses vinculados al sistema de salud único

Introdução: Os índices de parto cesariano vêm aumentando mundialmente e ultrapassando, em muitos locais, os índices de parto vaginal. No entanto, tais índices nem sempre refletem a indicação real do procedimento. Objetivo: analisar as taxas de cesáreas de hospitais vinculados à Secretaria Municipal do Estado do Paraná no ano de 2021, exceto universitários, através da Classificação de Robson. Métodos: Trata-se de um estudo descritivo, transversal e de abordagem quantitativa, foram avaliadas 356 instituições de saúde do estado do Paraná, os dados foram coletados no site do Departamento de Informática do Sistema Único de Saúde (DATASUS), através do Sistema de Informações sobre Nascidos Vivos (SINASC) via tabulador oficial do Ministério da Saúde- Tabwim, no ano de 2021. Resultados: Os maiores índices segundo Classificação de Robson, foram de mulheres entre 20 à 34 anos, com companheiro, escolaridade de oito anos ou mais de estudo, multíparas com filhos vivos anteriores a última gestação, sendo estas a termo, com gravidez única e sem anomalias congênitas, além da realização de sete consultas de pré-natal com seu início no primeiro trimestre de gestação. Conclusão: o parto cesariano ultrapassa os índices do parto vaginal. Podemos evidenciar a importância que os profissionais de saúde possuem para revertermos essa realidade através da educação em saúde, e no desenvolvimento de estratégias que minimizem esses números expressivos de cesarianas.

Introduction: The rate of cesarean delivery has been increasing worldwide and in many places surpassing the rate of vaginal delivery. However, such indexes do not always reflect the actual indication of the procedure. Objective: To analyze the rates of cesarean sections of hospitals linked to the Municipal Secretariat of the State of Paraná in the year 2021, except university, through the Robson Classification. Methods: It is a descriptive, cross-sectional and quantitative approach study, 356 health institutions of the state of Paraná were evaluated, the data were collected on the website of the Department of Informatics of the Unified Health System (DATASUS), through the Information System on Live Births (SINASC) via the official tabler of the Ministry of Health- Tabwim, in the year 2021. Results: The highest rates according to the Robson Classification were women between 20 and 34 years of age, with a partner, eight years of schooling or more of study, multiparous with living children prior to the last pregnancy, these being full-term, with single pregnancy and without congenital anomalies, in addition to having seven prenatal appointments with their start in the first trimester of gestation. Conclusion: cesarean delivery exceeds the rates of vaginal delivery. We can highlight the importance that health professionals have to reverse this reality through health education, and in the development of strategies that minimize these expressive numbers of cesareans.

Introducción: las tasas de natalidad cesárea han estado aumentando en todo el mundo y han superado las tasas de natalidad vaginal en muchos lugares. Sin embargo, esos índices no siempre reflejan la indicación real del procedimiento. Objetivo: analizar las tasas de cesárea hospitalaria vinculada a la Secretaría Municipal del Estado de Paraná en 2021, excepto los graduados universitarios, a través de la Clasificación de Robson. Métodos: Se trata de un estudio descriptivo, transversal y cuantitativo; se evaluaron 356 instituciones de salud del estado de Paraná; los datos se recogieron en el sitio web del Departamento de Información del Sistema de Salud Pública (DATASUS), a través del Sistema de Información sobre Aves Vivas (SINASC) a través del tabulador oficial del Ministerio de Salud ­ Tabwim, en 2021. Resultados: Los índices más altos según la Clasificación de Robson fueron las mujeres de 20 a 34 años de edad, con parejas, escolaridad durante ocho años o más de estudio, multiples con hijos vivos antes del último embarazo, con un solo embarazo y sin anomalías congénitas, y también se realizaron siete consultas prenatales con su inicio en el primer trimestre de gestación. Conclusión: el parto cesárea excede las tasas vaginales de nacimiento. Podemos destacar la importancia que tienen los profesionales de la salud para revertir esta realidad a través de la educación sanitaria, y en el desarrollo de estrategias que minimicen este número expresivo de cesáreas.

Avaliação da completude dos dados do sistema de informação sobre nascidos vivos de imigrantes e brasileiros no estado do Paraná, 2014-2019 / Evaluation of the completeness of data in the information system on live births of immigrants and brazilians in the state of Paraná, 2014-2019 / Evaluación de la completitud de los datos del sistema de información sobre nacidos vivos de inmigrantes y brasileños en el estado de Paraná, 2014-2019

Avaliar a completude dos dados no SINASC do Paraná, entre 2014 a 2019, de imigrantes e brasileiros. Método: Estudo transversal, retrospectivo, de análise de completude dos dados de nascimento do ano de 2014 a 2019 no estado do Paraná. Foram critérios de análise: excelente (menos de 5% de incompletude); bom (5% a 10%); regular (10% a 20%); ruim (20% a 50%); e muito ruim (acima de 50%). Resultados: Foram registrados no Brasil 948.316 nascidos vivos, dos quais 935.629 eram de mães brasileiras e 12.867 de mães imigrantes de diversas nacionalidades. Dentre as variáveis, os campos incompletos de mães brasileiras somaram 50.243 (5,37%) e de imigrantes 696 (5,41%), demonstrando um bom preenchimento do banco. Conclusão: Foi possível verificar que o banco de dados SINASC mostrou-se confiável e com baixas incompletudes entre os anos de 2014 a 2019 no estado do Paraná, independente da naturalidade da mãe. Porém, dados incompletos referentes às imigrantes ainda são maiores comparados aos de mulheres brasileiras, podendo ser resultado de uma falta de capacitação dos profissionais da saúde para a comunicação com as mães imigrantes que não falam a língua nativa, não coletando os dados de forma adequada e completa.

To evaluate the completeness of data in the SINASC of Paraná, from 2014 to 2019, of immigrants and Brazilians. Method: Cross-sectional, retrospective study, of analysis of completeness of birth data from the year 2014 to 2019 in the state of Paraná. Analysis criteria were: excellent (less than 5% incompleteness); good (5% to 10%); fair (10% to 20%); bad (20% to 50%); and very bad (above 50%). Results: In Brazil, 948,316 live births were registered, of which 935,629 were from Brazilian mothers and 12,867 from immigrant mothers of various nationalities. Among the variables, the incomplete fields of Brazilian mothers totaled 50,243 (5.37%) and of immigrants 696 (5.41%), de- monstrating a good completion of the bank. Conclusion: It was possible to verify that the SINASC database proved to be reliable and with low incompleteness between the years 2014 to 2019 in the state of Paraná, regardless of the mother's naturality. However, in- complete data referring to immigrants are still higher compared to those of Brazilian wo- men, which may be the result of a lack of training of health professionals for communi- cation with immigrant mothers who do not speak the native language, not collecting the data properly and completely.

Evaluar la completitud de datos en el SINASC de Paraná, de 2014 a 2019, de inmigrantes y brasileños. Método: Estudio transversal, retrospectivo, de análisis de completitud de datos de nacimiento del año 2014 a 2019 en el estado de Paraná. Los criterios de análisis fueron: excelente (menos de 5% de incompletitud); bueno (5% a 10%); regular (10% a 20%); malo (20% a 50%); y muy malo (más de 50%). Resultados: En Brasil se registraron 948.316 nacidos vivos, de los cuales 935.629 eran de madres brasileñas y 12.867 de madres inmigrantes de diversas nacionalidades. Dentre as variá- veis, os campos incompletos de mães brasileiras somaram 50.243 (5,37%) e de imigrantes 696 (5,41%), demonstrando um bom preenchimento do banco. Conclusão: Foi possível verificar que o banco de dados SINASC mostrou-se confiável e com baixa incompletudes entre os anos de 2014 a 2019 no estado do Paraná, independentemente da naturalidade da mãe. Sin embargo, los datos incompletos referidos a las inmigrantes siguen siendo más altos en comparación con los de las mujeres brasileñas, lo que puede ser el resultado de la falta de formación de los profesionales de la salud para la comunicación con las madres inmigrantes que no hablan el idioma nativo, no recogiendo los datos de forma adecuada y completa.

Controle da dengue no contexto da pandemia da covid-19: um olhar dos agentes de combate às endemias / Dengue control in the context of the covid-19 pandemic: a view from the endemic disease control agents / Control del dengue en el contexto de la pandemia del covid-19: una mirada a los agentes de combate a las enfermedades endémicas

Objetivo: Compreender a atuação dos Agentes de Combate às Endemias (ACE) no desenvolvimento de estratégias para o controle da dengue no contexto da covid-19. Metodologia: Estudo descritivo-exploratório qualitativo, desenvolvido junto a oito ACE de três Unidades Básicas de Saúde (UBS) com alto índice de infestação do Aedes aegypti, localizadas em um município paranaense. Os dados foram coletados mediante entrevista individual, conduzida por um roteiro semiestruturado com dez questões, e submetidos à análise de conteúdo, recorrendo-se à análise lexicográfica na modalidade de classificação hierárquica descendente. Resultados: Foram visualizados inúmeros desafios enfrentados pelos ACE nas UBS durante a pandemia da covid-19, bem como as mudanças ocasionadas no seu processo de trabalho. Percebeu-se a importância da visita domiciliar de rastreio dos focos de dengue, ressaltando as barreiras sanitárias e sociais vivenciadas pelos ACE. Também se notou a relevância desses profissionais no enfrentamento da arbovirose, especialmente pelo reconhecimento profissional na Política Nacional de Atenção Básica. Conclusão: A atuação dos ACE foi perceptível na prevenção e no controle da dengue, principalmente em meio à pandemia. Apesar do infortúnio causado pela covid-19, os ACE continuaram exercendo suas funções nas UBS, responsabilizando-se pela vigilância epidemiológica e cumprindo as normas e diretrizes estabelecidas na época.

Objective: To understand the role of the Agents of Combat to Endemic Diseases (ACE) in the development of strategies for dengue control in the context of the covid-19 pandemic. Methodology: A qualitative descriptive-exploratory study was carried out with eight ECs from three Basic Health Units (BHU) with high rates of Aedes aegypti infestation, located in a city in Paraná. Data were collected through individual interviews, conducted by a semi-structured script with ten questions, and submitted to content analysis, using lexicographic analysis in the descending hierarchical classification mode. Results: Numerous challenges faced by the CHWs in the PHU during the covid- 19 pandemic were visualized, as well as the changes caused in their work process. The importance of home visits for screening of dengue outbreaks was perceived, highlighting the health and social barriers experienced by the CHWs. The relevance of these professionals in confronting arbovirosis was also noted, especially by the professional recognition in the National Primary Care Policy. Conclusion: The performance of the CHAs was noticeable in the prevention and control of dengue, especially in the midst of the pandemic. Despite the misfortune caused by covid-19, the CHAs continued to exercise their functions in the PHUs, taking responsibility for epidemiological surveillance and complying with the norms and guidelines established at the time.

Objetivo: Conocer el papel de los Agentes de Combate a Enfermedades Endémicas (ACE) en el desarrollo de estrategias de control del dengue en el contexto de la covid-19. Metodología: Estudio cualitativo descriptivo-exploratorio, desarrollado con ocho AE de tres Unidades Básicas de Salud (UBS) con altos índices de infestación por Aedes aegypti, localizadas en un municipio de Paraná. Los datos fueron recolectados a través de entrevistas individuales, conducidas por un guión semiestructurado con diez preguntas, y sometidos a análisis de contenido, utilizando análisis lexicográfico en la modalidad de clasificación jerárquica descendente. Resultados: Foram visualizados inúmeros desafios enfrentados pelos ACE nas UBS durante a pandemia da covid-19, bem como as mudanças ocasionadas no seu processo de trabalho. Percebeu-se a importância da visita domiciliar de rastreio dos focos de dengue, ressaltando as barreiras sanitárias e sociais vivenciadas pelos ACE. Também foi notada a relevância destes profissionais no enfrentamento à arbovirosis, nomeadamente através do reconhecimento profissional na Política Nacional de Atenção Primaria de Saúde. Conclusão: A atuação dos ACE foi perceptível na prevenção e no controle do dengue, principalmente em meio à pandemia. Apesar do infortunio causado pelo covid-19, os ACE continuaram a exercer as suas funções nas UPH, responsabilizándose pela vigilância epidemiológica e cumprindo as normas e orientações estabelecidas naquela época.

Ter um fiho durante a pós-graduação em enfermagem: perspectivas e desafios / Having a child during graduate nursing: perspectives and challenges / Tener un hijo durante la carrera de enfermería: perspectivas y retos

Objetivo: compreender como enfermeiras percebem a vivência de uma gravidez e os primeiros meses após o nascimento de um filho durante o mestrado/doutorado. Método: estudo qualitativo, exploratório-descritivo, desenvolvido com nove pós-graduandas em enfermagem de uma universidade pública do estado do Paraná. A coleta de dados foi realizada nos meses de agosto e setembro de 2022, a partir de entrevistas individuais semiestruturadas que foram audiogravadas, transcritas e submetidas à análise de conteúdo, modalidade temática proposta por Bardin. Resultados: emergiram três categorias temáticas: 1) Enfrentando desafios: conciliar tarefas é uma necessidade; 2) Rede de apoio como facilitadora na conciliação da maternidade com os estudos e; 3) Aumento do tempo de licença-maternidade, flexibilização e apoio interno para inclusão de mulheres mães na ciência. Conclusão: as vivências da maternidade vivenciadas por mulheres na pós-graduação foram pautadas na sobrecarga das mães pesquisadoras, repercutindo em atrasos no cumprimento de prazos, dificuldade em manter a amamentação e preocupação com a saúde dos filhos, refletindo em escolhas e renúncias da maternidade nesta etapa da vida.

Objective: to understand how nurses perceive the experience of pregnancy and the first months after the birth of a child during their master's/doctoral studies. Method: qualitative, exploratory-descriptive study, developed with nine graduate students in nursing at a public university in the state of Paraná. Data collection was carried out in August and September 2022, based on semi-structured individual interviews that were audio-recorded, transcribed and submitted to content analysis, the thematic modality proposed by Bardin. Results: three thematic categories emerged: 1) Facing challenges: reconciling tasks is a necessity; 2) Support network as a facilitator in reconciling motherhood with studies and; 3) Increased maternity leave, flexibility and internal support for the inclusion of women mothers in science. Conclusion: the experiences of motherhood experienced by women in graduate school were based on the overload of research mothers, resulting in delays in meeting deadlines, difficulty in maintaining breastfeeding and concern for the health of their children, reflecting on choices and waivers of motherhood in this life stage.

Objetivo: comprender cómo las enfermeras perciben la experiencia del embarazo y los primeros meses después del nacimiento de un hijo durante sus estudios de maestría/doctorado. Método: estudio cualitativo, exploratorio-descriptivo, desarrollado con nueve estudiantes de postgrado en enfermería de una universidad pública del estado de Paraná. La recolección de datos se realizó en agosto y septiembre de 2022, a partir de entrevistas individuales semiestructuradas que fueron grabadas en audio, transcritas y sometidas a análisis de contenido, modalidad temática propuesta por Bardin. Resultados: emergieron tres categorías temáticas: 1) Enfrentar desafíos: conciliar tareas es una necesidad; 2) Red de apoyo como facilitadora en la conciliación de la maternidad con los estudios y; 3) Aumento de la licencia de maternidad, flexibilidad y apoyo interno para la inclusión de mujeres madres en la ciencia. Conclusión: las experiencias de maternidad vividas por las mujeres en el posgrado se basaron en la sobrecarga de las madres investigadoras, resultando en retrasos en el cumplimiento de los plazos, dificultad para mantener la lactancia materna y preocupación por la salud de sus hijos, reflexionando sobre las opciones y renuncias de la maternidad en esta etapa de la vida.

Combined use of systemic quercetin, glutamine and alpha-tocopherol attenuates myocardial fibrosis in diabetic rats.

This study aimed to analyze the effects of the quercetin (100 mg/kg), 1% glutamine and 1% α-tocopherol antioxidants in the myocardium of rats with streptozotocin-induced diabetes mellitus. Twenty male rats were subdivided into four groups (n = 5): N (normoglycemic); D (diabetic); NT (normoglycemic treated with antioxidants); and DT (diabetic treated with antioxidants) treated for 60 days. Clinical parameters, oxidative stress markers, inflammatory cytokines, myocardial collagen fibers and immunoexpression of superoxide dismutase 1 (SOD-1), glutathione peroxidase-1 (GPx-1), interleukin-1ß (IL-1-ß), transforming growth factor-beta (TGF-ß), and fibroblast growth factor-2 (FGF-2) were evaluated. Results showed reduced body weight, hyperphagia, polydipsia and hyperglycemic state in groups D and DT. The levels of glutathione (GSH) were higher in NT and DT compared to N (p < 0.01) and D (p < 0.001) groups, respectively. Greater GSH levels were found in DT when compared to N animals (p < 0.001). In DT, there was an increase in IL-10 in relation to N, D and NT (p < 0.05), while GPx-1 expression was similar to N and lower compared to D (p < 0.001). TGF-ß expression in DT was greater than N (p < 0.001) group, whereas FGF-2 in DT was higher than in the other groups (p < 0.001). A significant reduction in collagen fibers (type I) was found in DT compared to D (p < 0.05). The associated administration of quercetin, glutamine and α-tocopherol increased the levels of circulating interleukin-10 (IL-10) and GSH, and reduced the number of type I collagen fibers. Combined use of systemic quercetin, glutamine and alpha-tocopherol attenuates myocardial fibrosis in diabetic rats.

Quercetin increases bioavailability of nitric oxide in the jejunum of euglycemic and diabetic rats and induces neuronal plasticity in the myenteric plexus.

Considering the antioxidant, neuroprotective, inflammatory and nitric oxide modulatory actions of quercetin, the aim of this study was to test the effect of quercetin administration in drinking water (40 mg/day/rat) on neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), overall population of myenteric neurons (HuC/D) and nitric oxide (NO) levels in the jejunal samples from diabetic rats. Male Wistar rats were distributed into four groups (8 rats per group): euglycemic (E), euglycemic administered with quercetin (E+Q), diabetic (D) and diabetic administered with quercetin (D+Q). Rats were induced to diabetes with streptozotocin (35mg/kg/iv) and, after 120 days, the proximal jejunum were collected and processed for immunohistochemical (VIP, nNOS and HuC/D) and chemiluminescence (quantification of tissue NO levels) techniques. Diabetes mellitus reduced the number of nNOS-IR (immunoreactive) (p <0.05) and HuC/D-IR (p <0.001) neurons, however, promoted an increased morphometric area of nNOS-IR neurons (p <0.001) and VIP-IR varicosities (p <0.05). In D+Q group, neuroplasticity effects were observed on HuC/D-IR neurons, accompanied by a reduction of cell body area of neurons nNOS- and VIP-IR varicosities (p <0.05). The NO levels were increased in the E+Q (p <0.05) and D+Q group (p <0.001) compared to the control group. In conclusion, the results showed that quercetin supplementation increased the bioavailability of NO in the jejunum in euglycemic and mitigate the effects of diabetes on nNOS-IR neurons and VIP-IR varicosities in the myenteric plexus of diabetic rats.

QUERCETIN SUPPLEMENTATION PREVENTS CHANGES IN THE SEROTONIN AND CASPASE-3 IMMUNOREACTIVE CELLS OF THE JEJUNUM OF DIABETIC RATS.

BACKGROUND: Serotonin (5-HT) is present in the epithelial enterochromaffin cells (EC), mast cells of the lamina propria and enteric neurons. The 5-HT is involved in regulating motility, secretion, gut sensation, immune system and inflammation. OBJECTIVE: Evaluate the effects of diabetes and quercetin supplementation on serotoninergic cells and its cell loss by apoptosis in jejunal mucosa of streptozotocin-induced diabetic rats (STZ-rats). METHODS: Twenty-four male Wistar rats were divided into four groups: normoglycemic (C), normoglycemic supplemented with 40 mg/day quercetin (Q), diabetic (D) and diabetic supplemented with 40 mg/day quercetin (DQ). After 120 days, the jejunum was collected and fixated in Zamboni's solution for 18 h. After obtaining cryosections, immunohistochemistry was performed to label 5-HT and caspase-3. Quantification of 5-HT and caspase-3 immunoreactive (IR) cells in the lamina propria, villi and crypts were performed. RESULTS: The diabetic condition displayed an increase of the number of 5-HT-IR cells in villi and crypts, while decreased number of these cells was observed in lamina propria in the jejunum of STZ-rats. In the diabetic animals, an increased density of apoptotic cells in epithelial villi and crypts of the jejunum was observed, whereas a decreased number of caspase-3-IR cells was observed in lamina propria. Possibly, quercetin supplementation slightly suppressed the apoptosis phenomena in the epithelial villi and crypts of the STZ-rats, however the opposite effect was observed on the 5-HT-IR cells of the lamina propria. Quercetin supplementation on healthy animals promoted few changes of serotoninergic function and apoptotic stimuli. CONCLUSION: These results suggest that quercetin supplementation mostly improved the serotonergic function affected by diabetes maybe due to antioxidant and anti-inflammatory properties of quercetin.

A suplementação com quercetina previne mudanças em células imunorreativas à serotonina e caspase-3 do jejuno de ratos diabéticos / Quercetin supplementation prevents changes in the serotonin and caspase-3 immunoreactive cells of the jejunum of diabetic rats

ABSTRACT BACKGROUND: Serotonin (5-HT) is present in the epithelial enterochromaffin cells (EC), mast cells of the lamina propria and enteric neurons. The 5-HT is involved in regulating motility, secretion, gut sensation, immune system and inflammation. OBJECTIVE: Evaluate the effects of diabetes and quercetin supplementation on serotoninergic cells and its cell loss by apoptosis in jejunal mucosa of streptozotocin-induced diabetic rats (STZ-rats). METHODS: Twenty-four male Wistar rats were divided into four groups: normoglycemic (C), normoglycemic supplemented with 40 mg/day quercetin (Q), diabetic (D) and diabetic supplemented with 40 mg/day quercetin (DQ). After 120 days, the jejunum was collected and fixated in Zamboni's solution for 18 h. After obtaining cryosections, immunohistochemistry was performed to label 5-HT and caspase-3. Quantification of 5-HT and caspase-3 immunoreactive (IR) cells in the lamina propria, villi and crypts were performed. RESULTS: The diabetic condition displayed an increase of the number of 5-HT-IR cells in villi and crypts, while decreased number of these cells was observed in lamina propria in the jejunum of STZ-rats. In the diabetic animals, an increased density of apoptotic cells in epithelial villi and crypts of the jejunum was observed, whereas a decreased number of caspase-3-IR cells was observed in lamina propria. Possibly, quercetin supplementation slightly suppressed the apoptosis phenomena in the epithelial villi and crypts of the STZ-rats, however the opposite effect was observed on the 5-HT-IR cells of the lamina propria. Quercetin supplementation on healthy animals promoted few changes of serotoninergic function and apoptotic stimuli. CONCLUSION: These results suggest that quercetin supplementation mostly improved the serotonergic function affected by diabetes maybe due to antioxidant and anti-inflammatory properties of quercetin.

RESUMO CONTEXTO: A serotonina (5-HT) está presente nas células epiteliais enterocromafins (CE), nos mastócitos da lâmina própria e nos neurônios entéricos. A 5-HT está envolvida na regulação da motilidade, secreção, nocepção intestinal, sistema imunológico e inflamação. Objetivo: Avaliar os efeitos do diabetes e da suplementação de quercetina sobre a função serotoninérgica e a perda celular por apoptose na mucosa jejunal de ratos diabéticos induzidos por estreptozotocina (ratos STZ). MÉTODOS: Vinte e quatro ratos Wistar machos foram divididos em quatro grupos: normoglicêmico (C), normoglicêmico suplementado com quercetina 40 mg/dia (Q), diabético (D) e diabético suplementado com quercetina 40 mg/dia (DQ). Após 120 dias, o jejuno foi coletado e fixado na solução de Zamboni por 18 horas. Após a obtenção de cortes em criostato, a imuno-histoquímica foi realizada para marcar 5-HT e caspase-3. A quantificação de células imunorreativas (IR) à 5-HT e caspase-3 foram realizadas na lâmina própria, vilosidades e criptas. RESULTADOS: A condição diabética ocasionou um aumento do número de células 5-HT-IR nas vilosidades e criptas, enquanto que na lâmina própria houve uma redução dessas células, no jejuno de ratos STZ. Nos animais diabéticos, foi observada uma densidade aumentada de células apoptóticas no epitélio do jejuno, tanto nas vilosidades quanto nas criptas, por outro lado um número reduzido de células caspase-3-IR foi observado na lâmina própria. Possivelmente, a suplementação de quercetina suprimiu ligeiramente os fenômenos de apoptose no epitélio de vilosidades e criptas do jejuno de ratos STZ, no entanto, o efeito oposto foi observado nas células 5-HT-IR da lâmina própria. A suplementação com quercetina em animais saudáveis promoveu poucas alterações na função serotoninérgica e nos estímulos apoptóticos. CONCLUSÃO: Estes resultados sugerem que a suplementação de quercetina melhorou principalmente a função serotoninérgica afetada pelo diabetes, talvez devido às propriedades antioxidantes e anti-inflamatórias da quercetina.

Supplementation with l-glutathione improves oxidative status and reduces protein nitration in myenteric neurons in the jejunum in diabetic Rattus norvegicus.

Diabetes mellitus is a syndrome with multiple etiologies, characterized by chronic hyperglycemia that increases the production of reactive oxygen species and decreases antioxidant defenses. The present study evaluated oxidative stress parameters and protein nitration in myenteric neurons in the jejunum in diabetic rats supplemented with l-glutathione. Rats (90 days of age) were distributed into four groups (n = 6/group): normoglycemic (N), normoglycemic supplemented with l-glutathione (NGT), diabetic (D), and diabetic supplemented with l-glutathione (DGT). At 210 days of age, the animals were sacrificed, and the jejunum was collected, washed, and subjected to various procedures: tert-butyl hydroperoxide chemiluminescence (CL), determination of total antioxidant capacity (TAC), determination of catalase activity, quantification of nitric oxide (NO), and double-labeling of HuC/D-immunoreactive myenteric neurons and nitrotyrosine (3-NT). Diabetes increased oxidative stress in the jejunum in the D group, reflected by increases in lipid peroxidation, TAC, catalase activity, and NO. The D group exhibited an increase in the percentage of myenteric neurons that were double-labeled with 3-NT. Supplementation with l-glutathione did not cause differences in the average CL curves between the D and DGT groups, but reductions of TAC and catalase activity were observed. Supplementation with l-glutathione promoted a reduction of neurons that contained 3-NT in the DGT group. Diabetes mellitus promoted oxidative stress in the jejunum, and supplementation with l-glutathione improved oxidative status by preventing protein nitration in myenteric neurons in diabetic animals that received supplementation.

l-Glutamine supplementation promotes an improved energetic balance in Walker-256 tumor-bearing rats.

We evaluated the effects of supplementation with oral l-glutamine in Walker-256 tumor-bearing rats. A total of 32 male Wistar rats aged 54 days were randomly divided into four groups: rats without Walker-256 tumor, that is, control rats (C group); control rats supplemented with l-glutamine (CG group); Walker-256 tumor rats without l-glutamine supplementation (WT group); and WT rats supplemented with l-glutamine (WTG group). l-Glutamine was incorporated into standard food at a proportion of 2 g/100 g (2%). After 10 days of the experimental period, the jejunum and duodenum were removed and processed. Protein expression levels of key enzymes of gluconeogenesis, that is, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase, were analyzed by western blot and immunohistochemical techniques. In addition, plasma corticosterone, glucose, insulin, and urea levels were evaluated. The WTG group showed significantly increased plasma glucose and insulin levels ( p < 0.05); however, plasma corticosterone and urea remained unchanged. Moreover, the WTG group showed increased immunoreactive staining for jejunal phosphoenolpyruvate carboxykinase and increased expression of duodenal glucose-6-phosphatase. Furthermore, the WTG group presented with less intense cancer cachexia and slower tumor growth. These results could be attributed, at least partly, to increased intestinal gluconeogenesis and insulinemia, and better glycemia maintenance during fasting in Walker-256 tumor rats on a diet supplemented with l-glutamine.

Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation

Background: Interstitial cells of Cajal (ICC) are required for normal motility in the gastrointestinal tract. Depletion of ICC has been associated with diabetic gastroenteropathy. Aim: To determine the effect of quercertin supplementation on anoctamin-1 (Ano 1) immunoreactive ICC in the myenteric region (ICC-MY) and deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats. Methods: Thirty-two 90-day-old male Wistar rats were distributed into the following groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). Diabetes was induced by streptozotocin injection. After 120 days, preparations of the jejunal muscular and submucosal layers were immunostained for Ano1 to visualize ICC. Evaluation of the immunofluorescence intensity as well as density of ICC was performed. Results: The density of ICC-MY was 46% lower in group D compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After quercertin treatment, the densities of ICCMY were significantly higher in the DQ group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin in normoglycemic animals (CQ) compared with group C did not significantly change the ICC density (p > 0.05). Conclusions: In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action (AU)

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