ABSTRACT
Periodontitis is a widely prevalent oral disease around the world characterized by the disruption of the periodontal ligament and the subsequent development of periodontal pockets, as well as the loss of alveolar bone, and may eventually lead to tooth loss. This research aims to assess the suppressive impact of Eupatilin, a flavone obtained from Artemisia argyi, on osteoclastogenesis in vitro and periodontitis in vivo. We found that Eupatilin can efficiently obstruct the differentiation of Raw264.7 and bone marrow-derived macrophages (BMDMs) induced by RANKL, leading to the formation of mature osteoclasts. Consistently, bone slice resorption assay showed that Eupatilin significantly inhibited osteoclast-mediated bone resorption in a dose-dependent manner. Eupatilin also downregulated the expression of osteoclast-specific genes and proteins in Raw264.7 and BMDMs. RNA sequencing showed that Eupatilin notably downregulated the expression of Siglec-15. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified significantly enriched pathways in DEGs, including MAPK signaling pathway. And further mechanistic investigations confirmed that Eupatilin repressed MAPKs/NF-κBsignaling pathways. It was found that Siglec-15 overexpression reversed the inhibitory impact of Eupatilin on the differentiation of osteoclasts. Furthermore, activating MAPK signaling pathway reversed the downregulation of Siglec-15 and the inhibition of osteoclastogenesis by Eupatilin. To sum up, Eupatilin reduced the expression of Siglec-15 by suppressing MAPK signaling pathway, ultimately leading to the inhibition of osteoclastogenesis. Meanwhile, Eupatilin suppressed the alveolar bone resorption caused by experimentalperiodontitis in vivo. Eupatilin exhibits potential therapeutic effects in the treatment of periodontitis, rendering it a promising pharmaceutical agent.
Subject(s)
Alveolar Bone Loss , Flavonoids , Osteoclasts , Osteogenesis , Periodontitis , Animals , Mice , Osteogenesis/drug effects , RAW 264.7 Cells , Flavonoids/pharmacology , Flavonoids/therapeutic use , Alveolar Bone Loss/drug therapy , Osteoclasts/drug effects , Periodontitis/drug therapy , Mice, Inbred C57BL , Cell Differentiation/drug effects , Male , Macrophages/drug effects , MAP Kinase Signaling System/drug effects , Signal Transduction/drug effects , Artemisia/chemistry , RANK Ligand/metabolismABSTRACT
Periodontitis is one of the most prevalent chronic inflammatory diseases that may eventually lead to the loss of teeth. Macrophage polarization plays an important role in the development of periodontitis, and several naturally occurring food compounds have recently been reported to regulate macrophage polarization. In this study, we aimed to investigate the therapeutic potential of sulforaphene (SFE) in macrophage polarization and its impact on periodontitis. Through in vitro and in vivo experiments, our study demonstrated that SFE effectively inhibits M1 polarization while promoting M2 polarization, ultimately leading to the suppression of periodontitis. Transcriptome sequencing showed that SFE significantly upregulated the expression of dendritic cell immunoreceptor (DCIR, also known as CLEC4A2). We further validated the crucial role of DCIR in macrophage polarization through knockdown and overexpression experiments and demonstrated that SFE regulates macrophage polarization by upregulating DCIR expression. In summary, the results of this study suggest that SFE can regulate macrophage polarization and inhibit periodontitis. Moreover, this research identified DCIR (dendritic cell immunoreceptor) as a potential novel target for regulating macrophage polarization. These findings provide new insights into the treatment of periodontitis and other immune-related diseases.
Subject(s)
Lectins, C-Type , Periodontitis , Humans , Lectins, C-Type/genetics , Lectins, C-Type/metabolism , Macrophages/metabolism , Periodontitis/drug therapy , Periodontitis/metabolism , Dendritic Cells/metabolismABSTRACT
Dysregulation of non-coding RNAs (ncRNAs) has been proved to play important roles in oral squamous cell carcinoma (OSCC). This study aimed to determine the combined role of lncRNA TUG1, miR-593-3p, and MAPK signaling in oral squamous cell carcinoma (OSCC) development. Here, we found that TUG1 was up-regulated in OSCC tissues and cell lines. Silencing TUG1 suppressed proliferation migration, invasion and promoted apoptosis of OSCC cells. We also validated that knockdown of TUG1 suppressed MAPK signaling pathway and inhibited EMT process in OSCC cells. Then, a novel LncRNA TUG1/ miR-593-3p/MAPK axis was verified to rescue cell viability in OSCC cells. Mechanistically, miR-593-3p bound to lncRNA TUG1, and lncRNA TUG1 positively regulated MAPK related proteins through acting as RNA sponger for miR-593-3p. Further gain- and loss-of-function experiments evidenced that the protective effects of lncRNA TUG1 knock-down on OSCC cells were abrogated by silencing miRNA-593-3p. The OSCC nude mice model experiments demonstrated that depletion of TUG1 further inhibited tumor growth. In conclusion, appropriate diagnostic biomarkers and therapies for OSCC can be identified by targeting the TUG1/miR-593-3p/MAPK axis.
Subject(s)
MicroRNAs , Mouth Neoplasms , RNA, Long Noncoding , Squamous Cell Carcinoma of Head and Neck , Animals , Cell Line, Tumor , Cell Proliferation/genetics , Humans , Mice , Mice, Nude , MicroRNAs/genetics , Mouth Neoplasms/genetics , Mouth Neoplasms/pathology , RNA, Long Noncoding/genetics , Signal Transduction , Squamous Cell Carcinoma of Head and Neck/genetics , Squamous Cell Carcinoma of Head and Neck/pathologyABSTRACT
The 2019 novel coronavirus pandemic (COVID-19) negatively affected global public health and socioeconomic development. Lockdowns and travel restrictions to contain COVID-19 resulted in reduced human activity and decreased anthropogenic emissions. However, the secondary effects of these restrictions on the biophysical environment are uncertain. Using remotely sensed big data, we investigated how lockdowns and traffic restrictions affected China's spring vegetation in 2020. Our analyses show that travel decreased by 58% in the first 18 days following implementation of the restrictions across China. Subsequently, atmospheric optical clarity increased and radiation levels on the vegetation canopy were augmented. Furthermore, the spring of 2020 arrived 8.4 days earlier and vegetation 17.45% greener compared to 2015-2019. Reduced human activity resulting from COVID-19 restrictions contributed to a brighter, earlier, and greener 2020 spring season in China. This study shows that short-term changes in human activity can have a relatively rapid ecological impact at the regional scale.
ABSTRACT
Membrane proteins are crucial for biological processes, and many of them are important to drug targets. Understanding the three-dimensional structures of membrane proteins are essential to evaluate their bio-function and drug design. High-purity membrane proteins are important for structural determination. Membrane proteins have low yields and are difficult to purify because they tend to aggregate. We summarized membrane protein expression systems, vectors, tags, and detergents, which have deposited in the Protein Data Bank (PDB) in recent four-and-a-half years. Escherichia coli is the most expression system for membrane proteins, and HEK293 cells are the most commonly cell lines for human membrane protein expression. The most frequently vectors are pFastBac1 for alpha-helical membrane proteins, pET28a for beta-barrel membrane proteins, and pTRC99a for monotopic membrane proteins. The most used tag for membrane proteins is the 6×His-tag. FLAG commonly used for alpha-helical membrane proteins, Strep and GST for beta- barrel and monotopic membrane proteins, respectively. The detergents and their concentrations used for alpha-helical, beta-barrel, and monotopic membrane proteins are different, and DDM is commonly used for membrane protein purification. It can guide the expression and purification of membrane proteins, thus contributing to their structure and bio function studying.
Subject(s)
Databases, Protein , Escherichia coli , Gene Expression , Membrane Proteins , Recombinant Fusion Proteins , Escherichia coli/genetics , Escherichia coli/metabolism , Membrane Proteins/biosynthesis , Membrane Proteins/chemistry , Membrane Proteins/genetics , Membrane Proteins/isolation & purification , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/isolation & purificationABSTRACT
BACKGROUND/AIMS: Human dental pulp-derived mesenchymal stromal cells (hDPSCs) are promising seed cells for tissue engineering due to their easy accessibility and multi-lineage differentiation. Pannexin3 (Panx3) plays crucial roles during bone development and differentiation. The aim of the present study was to investigate the effect of Panx3 on osteogenesis of hDPSCs and the underlying mechanism. METHODS: Utilizing qRT-PCR, Western blot, and immunohistochemistry, we explored the change of Panx3 during osteogenic differentiation of hDPSCs. Next, hDPSCs with loss (Panx3 knockdown) and gain (Panx3 overexpression) of Panx3 function were developed to investigate the effects of Panx3 on osteogenic differentiation of hDPSC and the underlying mechanism. Finally, a commercial ß-TCP scaffold carrying Panx3-modified hDPSCs was utilized to evaluate bone defect repair. RESULTS: Panx3 was upregulated during osteogenic differentiation in a time-dependent manner. Panx3 overexpression promoted osteogenic differentiation of hDPSCs, whereas depletion of Panx3 resulted in a decline of differentiation, evidenced by upregulated expression of mineralization-related markers, increased alkaline phosphatase (ALP) activity, and enhanced ALP and Alizarin red staining. Panx3 was found to interact with the Wnt/ß-catenin signaling pathway, forming a negative feedback loop. However, Wnt/ß-catenin did not contribute to enhancement of osteogenic differentiation as observed in Panx3 overexpression. Moreover, Panx3 promoted osteogenic differentiation of hDPSCs via increasing ERK signaling pathway. Micro-CT and histological staining results showed that Panx3-modified hDPSCs significantly improved ossification of critical-sized bone defects. CONCLUSION: These findings suggest that Panx3 is a crucial modulator of hDPSCs differentiation.
Subject(s)
Connexins/genetics , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells/cytology , Osteogenesis , Skull/injuries , Up-Regulation , Adolescent , Adult , Animals , Cell Differentiation , Cells, Cultured , Connexins/metabolism , Dental Pulp/cytology , Fractures, Bone/pathology , Fractures, Bone/therapy , Gene Expression Regulation, Developmental , Humans , MAP Kinase Signaling System , Male , Mesenchymal Stem Cell Transplantation/methods , Mesenchymal Stem Cells/metabolism , Rats , Rats, Sprague-Dawley , Skull/pathology , Wnt Signaling Pathway , Young AdultABSTRACT
Haploinsufficiency of the runt-related transcription factor 2 (Runx2) gene is widely known to be responsible for cleidocranial dysplasia (CCD). To date, more than 190 mutations in Runx2 gene have been reported to be related to CCD. In this study, a novel mutation of Runx2 gene was observed in a female with CCD. Genomic DNA was extracted from peripheral venous blood of the proband and eleven members of her family. Genetic testing on these twelve people identified a novel missense mutation (c.895 T>C, Y299H) in exon 5 of the RUNX2 gene in the proband. This mutation results in an amino acid change at codon 895 (P.Tyr 299 His.) from a tryptophan codon (TAT) to a histidine codon (CAT). Our finding may further extend the known mutation spectrum of the RUNX2 gene, and facilitate prenatal genetic diagnosis of CCD in the future.
Subject(s)
Cleidocranial Dysplasia/genetics , Core Binding Factor Alpha 1 Subunit/genetics , Mutation, Missense , Sequence Analysis, DNA/methods , Adult , Amino Acid Substitution , Exons , Female , Genetic Predisposition to Disease , Histidine/genetics , Humans , Pedigree , Tryptophan/geneticsABSTRACT
Human dental pulp cells (HDPCs) play a crucial role in dental pulp inflammation. Pannexin 3 (Panx3), a member of Panxs (Pannexins), has been recently found to be involved in inflammation. However, the mechanism of Panx3 in human dental pulp inflammation remains unclear. In this study, the role of Panx3 in inflammatory response was firstly explored, and its potential mechanism was proposed. Immunohistochemical staining showed that Panx3 levels were diminished in inflamed human and rat dental pulp tissues. In vitro, Panx3 expression was significantly down-regulated in HDPCs following a TNF-α challenge in a concentration-dependent way, which reached the lowest level at 10 ng/ml of TNF-α. Such decrease could be reversed by MG132, a proteasome inhibitor. Unlike MG132, BAY 11-7082, a NF-κB inhibitor, even reinforced the inhibitory effect of TNF-α. Quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to investigate the role of Panx3 in inflammatory response of HDPCs. TNF-α-induced pro-inflammatory cytokines, interleukin (IL)-1ß and IL-6, were significantly lessened when Panx3 was overexpressed in HDPCs. Conversely, Panx3 knockdown exacerbated the expression of pro-inflammatory cytokines. Moreover, Western blot, dual-luciferase reporter assay, immunofluorescence staining, qRT-PCR and ELISA results showed that Panx3 participated in dental pulp inflammation in a NF-κB-dependent manner. These findings suggested that Panx3 has a defensive role in dental pulp inflammation, serving as a potential target to be exploited for the intervention of human dental pulp inflammation.
Subject(s)
Connexins/metabolism , Dental Pulp/metabolism , Inflammation/metabolism , NF-kappa B/metabolism , Signal Transduction/physiology , Tumor Necrosis Factor-alpha/metabolism , Adolescent , Adult , Animals , Cells, Cultured , Female , Humans , Inflammation Mediators/metabolism , Interleukin-1beta/metabolism , Interleukin-6/metabolism , Male , Rats , Young AdultABSTRACT
Haploinsuffieiency of the runt-related transcription factor 2 (Runx2) gene is widely known to be responsible for cleidocranial dysplasia (CCD).To date,more than 190 mutations in Runx2 gene have been reported to be related to CCD.In this study,a novel mutation of Runx2 gene was observed in a female with CCD.Genomic DNA was extracted from peripheral venous blood of the proband and eleven members of her family.Genetic testing on these twelve people identified a novel missense mutation (c.895T>C,Y299H) in exon 5 of the RUNX2 gene in the proband.This mutation results in an amino acid change at codon 895 (P.Tyr 299 His.) from a tryptophan codon (TAT) to a histidine codon (CAT).Our finding may further extend the known mutation spectrum of the RUNX2 gene,and facilitate prenatal genetic diagnosis of CCD in the future.
ABSTRACT
OBJECTIVE: The aim of this study is to investigate the expression of pannexin3 (Panx3) in human odontoblast-like cells (hOBs) and its hemichannel function in mediating ATP release. METHODS: RT-PCR and immunofluorescence analysis were used to detect the expression of pannexins (Panxs) in human dental pulp tissue and cultured cells. To determine the role of Panx3 in ATP release, hOBs were infected with Panx3-overexpression lentivirus, Panx3-shRNA lentivirus or control lentivirus and then stimulated with cold buffer. Intracellular ATP was monitored using quinacrine, and then semi-quantitatively analyzed. In the meantime, the ATP release was quantitatively analyzed using the bioluminescence method when the cells were exposed to cold stimulus. RESULTS: Panx3 mRNA and protein were found in dental pulp tissue and cultured cells. Upon cold stimulus, intracellular ATP was released into the extracellular space. Overexpression of Panx3 accelerated ATP release, whereas inhibition of Panx3 suppressed this process. CONCLUSION: Panx3 hemichannel is expressed in human odontoblast-like cells and mediates ATP release into the extracellular space.
Subject(s)
Adenosine Triphosphate/metabolism , Connexins/biosynthesis , Odontoblasts/metabolism , Adolescent , Adult , Cell Differentiation/physiology , Cell Line , Cells, Cultured , Cold Temperature , Connexins/genetics , Dental Pulp/cytology , Dental Pulp/metabolism , Dentin Sensitivity/genetics , Dentin Sensitivity/metabolism , Gene Knockdown Techniques , Humans , Lentivirus/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Small Interfering/administration & dosage , RNA, Small Interfering/genetics , Young AdultABSTRACT
The aim of this study was to investigate the effects of different direct current intensities on dentine bonding effectiveness of Clearfil S(3) Bond and on cell viability of human dental pulp cells (HDPCs). Thirty-five-third molars were sectioned and ground to provide flat surfaces. Clearfil S(3) Bond was applied under different current conditions for 30 s and then resin composite was built up. Specimens were processed for microtensile bond strength (µTBS) testing and for nanoleakage investigation using scanning electron microscopy. Primary HDPCs isolated from premolars were stimulated with different intensities of electric current for 30 s. Then, cell viability was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Specimens bonded with application of electrical current intensities of 50, 60, 70, and 90 µA exhibited a significant increase in immediate µTBS compared with all other groups. Bonded interfaces prepared using electrically assisted current application showed reduced interfacial nanoleakage upon scanning electron microscopy. Electric current application, from 20 to 70 µA, had no effect on the viability of HDPCs. This study provides further evidence for its future clinical use.
Subject(s)
Dental Bonding/methods , Dentin-Bonding Agents/chemistry , Dentin/ultrastructure , Electricity , Resin Cements/chemistry , Adolescent , Cell Culture Techniques , Cell Proliferation , Cell Survival/physiology , Cells, Cultured , Coloring Agents , Composite Resins/chemistry , Dental Leakage/classification , Dental Materials/chemistry , Dental Pulp/cytology , Electric Stimulation , Electrochemical Techniques , Humans , Microscopy, Electron, Scanning , Stress, Mechanical , Surface Properties , Tensile Strength , Tetrazolium Salts , Thiazoles , Time FactorsABSTRACT
Sirtuin 6 (SIRT6) is a NAD-dependent deacetylase involved in lifespan regulation. To evaluate the effect of SIRT6 on osteogenesis, rat bone marrow mesenchymal stem cells (rBMSCs) with enhanced or reduced SIRT6 function were developed. We observed that SIRT6 knockdown significantly reduced the mRNA levels of several key osteogenic markers in vitro, including alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), and osteocalcin, while overexpression of SIRT6 enhanced their expression. Additionally, SIRT6 knockdown activated nuclear factor-κB (NF-κB) transcriptional activity and upregulated the expression of acetyl-NF-κB p65 (Lys310). The decreased osteogenic differentiation ability of rBMSCs could be partially rescued by the addition of NF-κB inhibitor BAY 11-7082. Furthermore, SIRT6 overexpression in rBMSCs combined with the use of collagen/chitosan/hydroxyapatite scaffold could significantly boost new bone formation in rat cranial critical-sized defects, as determined by microcomputed tomography and histological examination. These data confirm that SIRT6 is mainly located in the nuclei of rBMSCs and plays an essential role in their normal osteogenic differentiation, partly by suppressing NF-κB signaling.
Subject(s)
Cell Differentiation , Mesenchymal Stem Cells/physiology , NF-kappa B/metabolism , Sirtuins/physiology , Alkaline Phosphatase/genetics , Alkaline Phosphatase/metabolism , Animals , Bone Regeneration , Cell Adhesion , Cell Nucleus/enzymology , Cell Proliferation , Cells, Cultured , Core Binding Factor Alpha 1 Subunit/genetics , Core Binding Factor Alpha 1 Subunit/metabolism , Gene Expression , Humans , Male , Mesenchymal Stem Cell Transplantation , Osteocalcin/genetics , Osteocalcin/metabolism , Osteogenesis , Rats, Sprague-Dawley , Signal TransductionABSTRACT
OBJECTIVES: To evaluate the effect of the addition of epigallocatechin-3-gallate (EGCG) on the antibacterial and physical properties of glass ionomer cement (GIC). METHODS: A conventional GIC, Fuji IX, was used as a control. EGCG was incorporated into GIC at 0.1% (w/w) and used as the experimental group. Chlorhexidine (CHX) was added into GIC at 1% (w/w) as a positive control. The anti-biofilm effect of the materials was assessed by a colorimetric technique (MTT assay) and scanning electron microscopy (SEM). The leaching antibacterial activity of the materials on Streptococcus mutans was evaluated by an agar-diffusion test. The flexural strength of the materials was evaluated using a universal testing machine and the surface microhardness was measured using a microhardness tester. The fluoride-releasing property of the materials was tested by ion chromatography. RESULTS: The optical density (OD) values of the GIC-EGCG group were significantly decreased at 4h compared with the GIC group, but only a slightly decreased tendency was observed at 24h (P>0.05). No inhibition zones were detected in the GIC group during the study period. Significant differences were found between each group (P<0.05). Compared with the control group, there was a significant increase in the flexural strength and surface microhardness for the GIC-EGCG group (P<0.05). The fluoride ion release was not influenced by EGCG-incorporation (P>0.05). CONCLUSIONS: These findings suggested that GIC-containing 0.1% (w/w) EGCG is a promising restorative material with improved mechanical properties and a tendency towards preferable antibacterial properties. CLINICAL SIGNIFICANCE: Modification of the glass ionomer cements with EGCG to improve the antibacterial and physical properties showed some encouraging results. This suggested that the modification of GIC with EGCG might be an effective strategy to be used in the dental clinic. However, this was only an in vitro study and clinical trials would need to verify true outcomes.
Subject(s)
Anti-Bacterial Agents/pharmacology , Catechin/analogs & derivatives , Glass Ionomer Cements/pharmacology , Anti-Bacterial Agents/chemistry , Biofilms/drug effects , Cariostatic Agents/chemistry , Catechin/chemistry , Catechin/pharmacology , Chemical Phenomena , Chlorhexidine/chemistry , Chlorhexidine/pharmacology , Chromatography/methods , Colorimetry/methods , Coloring Agents , Dental Stress Analysis/instrumentation , Fluorides/chemistry , Glass Ionomer Cements/chemistry , Hardness , Humans , Light , Materials Testing , Microscopy, Electron, Scanning , Pliability , Spectrophotometry/methods , Streptococcus mutans/drug effects , Stress, Mechanical , Surface Properties , Tetrazolium Salts , ThiazolesABSTRACT
OBJECTIVES: To evaluate the influence of desensitising paste containing 8% arginine and calcium carbonate (Ar-Ca) on biofilm formation on dentine. METHODS: Dentine discs were cut from extracted third molars and divided into the following three groups: no treatment, pumice treatment and Ar-Ca treatment. Surface topography and roughness were examined using scanning electron microscopy (SEM) and non-contact 3D surface profiler. After sterilisation, samples were incubated with Streptococcus mutans (S. mutans) for 4 h, 24 h and 72 h. Bacterial adhesion and biofilm formation were analysed using SEM, whereas MTT and lactic acid production assays were used to analyse the metabolic activity of S. mutans. RESULTS: After polishing with either pumice or Ar-Ca, the surfaces of the samples became smoother than in the control group. The Ra values of the three experimental groups decreased significantly to 0.43 µm, 0.3 µm and 0.26 µm, respectively. Compared to the control group, fewer bacteria adhered to the dentine surface in the Ar-Ca group, while biofilm thickness decreased significantly for both groups after incubating for 24 h and 72 h. MTT and lactic acid production levers also showed a significant reduction in the Ar-Ca group. CONCLUSIONS: Ar-Ca appears to present antibiofilm efficacy and may provide a promising approach to combat bacterial infection in hypersensitive dentinal lesions. CLINICAL SIGNIFICANCE: As a clinical application of desensitising polishing paste, the paste containing 8% arginine and calcium carbonate could also inhibit the biofilm formation effectively.
Subject(s)
Arginine/pharmacology , Biofilms/drug effects , Calcium Carbonate/pharmacology , Dentifrices/pharmacology , Dentin Desensitizing Agents/pharmacology , Fluorides/pharmacology , Phosphates/pharmacology , Streptococcus mutans/drug effects , Adolescent , Adult , Anti-Bacterial Agents/pharmacology , Bacterial Adhesion/drug effects , Coloring Agents , Dental Prophylaxis/methods , Dentin/microbiology , Dentin/ultrastructure , Humans , Imaging, Three-Dimensional/methods , Lactic Acid/metabolism , Microbial Viability/drug effects , Microscopy, Electron, Scanning , Silicates/pharmacology , Streptococcus mutans/physiology , Tetrazolium Salts , Thiazoles , Time Factors , Young AdultABSTRACT
In this paper we developed two types of chitosan-based microspheres with and without biomimetic apatite coatings and compared their potential as injectable scaffolds for bone regeneration. The microspheres were obtained by emulsion cross-linking (E0) and coacervate precipitation (C0), respectively. They were then biomimetically coated with apatite to become E1 and C1 microspheres. The physicochemical properties and biocompatibility of the microspheres were characterized. Both E0 and C0 microspheres presented favorable ranges of diameter, density and Rockwell hardness. However, there were differences in the degree of cross-linking, shape, morphology, degradation rate, swelling rate, pH value after PBS immersion and the biocompatibility between E0 and C0. The apatite coating was successfully prepared for both C0 and E0, which enhanced the attachment, proliferation and differentiation of MC3T3-E1 cells. In conclusion, our results suggest the feasibility of using chitosan microspheres as a potential injectable scaffold. Both the preparation method and the biomimetic apatite coating contribute to their biological properties.