ABSTRACT
Subtle changes in the membrane potential of pulmonary arterial smooth muscle cells (PASMCs) are pivotal for controlling pulmonary vascular tone, e.g., for initiating Hypoxic Pulmonary Vasoconstriction, a vital mechanism of the pulmonary circulation. In our study, we evaluated the ability of the fluorescence resonance energy transfer (FRET)-based voltage-sensor Mermaid to detect such subtle changes in membrane potential. Mouse PASMCs were isolated and transduced with Mermaid-encoding lentiviral vectors before the acceptor/donor emission ratio was assessed via live cell FRET-imaging. Mermaid's sensitivity was tested by applying specific potassium chloride (KCl) concentrations. These KCl concentrations were previously validated by patch clamp recordings to induce depolarization with predefined amplitudes that physiologically occur in PASMCs. Mermaid's emission ratio dose-dependently increased upon depolarization with KCl. However, Mermaid formed unspecific intracellular aggregates, which limited the usefulness of this voltage sensor. When analyzing the membrane rim only to circumvent these unspecific signals, Mermaid was not suitable to resolve subtle changes in the membrane potential of ≤10 mV. In summary, we found Mermaid to be a suitable alternative for reliably detecting qualitative membrane voltage changes of more than 10 mV in primary mouse PASMCs. However, one should be aware of the limitations associated with this voltage sensor.
Subject(s)
Fluorescence Resonance Energy Transfer , Membrane Potentials , Myocytes, Smooth Muscle , Animals , Fluorescence Resonance Energy Transfer/methods , Mice , Myocytes, Smooth Muscle/metabolism , Pulmonary Artery/physiology , Potassium Chloride/pharmacology , Mice, Inbred C57BLABSTRACT
Legionella pneumophila is the primary causative agent of Legionnaires' disease. The mutant-type strain interrupted in the ORF7 gene region responsible for the lipopolysaccharide biosynthesis of the L. pneumophila strain Heysham-1, lacking the O-acetyl groups attached to the rhamnose of the core part, showed a higher surface polarity compared with the wild-type strain. The measurement of excitation energy transfer between fluorophores located on the surface of bacteria and eukaryotic cells showed that, at an early stage of interaction with host cells, the mutant exhibited weaker interactions with Acanthamoeba castellanii cells and THP-1-derived macrophages. The mutant displayed reduced adherence to macrophages but enhanced adherence to A. castellanii, suggesting that the O-acetyl group of the LPS core region plays a crucial role in facilitating interaction with macrophages. The lack of core rhamnose O-acetyl groups made it easier for the bacteria to multiply in amoebae and macrophages. The mutant induced TNF-α production more strongly compared with the wild-type strain. The mutant synthesized twice as many ceramides Cer(t34:0) and Cer(t38:0) than the wild-type strain. The study showed that the internal sugars of the LPS core region of L. pneumophila sg 1 can interact with eukaryotic cell surface receptors and mediate in contacting and attaching bacteria to host cells as well as modulating the immune response to infection.
Subject(s)
Legionella pneumophila , Legionnaires' Disease , Humans , Legionella pneumophila/genetics , Lipopolysaccharides/metabolism , Rhamnose/metabolism , Serogroup , Bacterial Proteins/metabolismABSTRACT
In contracting muscles, carbohydrates and fatty acids serve as energy substrates; the predominant utilization depends on the workload. Here, we investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in response to repeated training in a polygenic, paternally selected marathon mouse model (DUhTP), characterized by exceptional running performance and an unselected control (DUC), with both lines descended from the same genetic background. Both lines underwent three weeks of high-speed treadmill training or were sedentary. Both lines' muscles and plasma were analyzed. Muscle RNA was sequenced, and KEGG pathway analysis was performed. Analyses of muscle revealed no significant selection-related differences in muscle structure. However, in response to physical exercise, glucose and fatty acid oxidation were stimulated, lactate dehydrogenase activity was reduced, and lactate formation was inhibited in the marathon mice compared with trained control mice. The lack of lactate formation in response to exercise appears to be associated with increased lipid mobilization from peripheral adipose tissue in DUhTP mice, suggesting a specific benefit of lactate avoidance. Thus, results from the analysis of muscle metabolism in born marathon mice, shaped by 35 years (140 generations) of phenotype selection for superior running performance, suggest increased metabolic flexibility in male marathon mice toward lipid catabolism regulated by lactate dehydrogenase.
Subject(s)
L-Lactate Dehydrogenase , Muscles , Physical Conditioning, Animal , Animals , Male , Mice , L-Lactate Dehydrogenase/metabolism , Lactic Acid/metabolism , Metabolic Networks and Pathways , Muscles/metabolismABSTRACT
The metabolic changes associated with intrauterine growth restriction (IUGR) particularly affect the liver, which is a central metabolic organ and contributes significantly to the provision of energy and specific nutrients and metabolites. Therefore, our aim was to decipher and elucidate the molecular pathways of developmental processes mediated by miRNAs and mRNAs, as well as the metabolome in fetal liver tissue in IUGR compared to appropriate for gestational age groups (AGA). Discordant siblings representing the extremes in fetal weight at day 63 post conception (dpc) were selected from F2 fetuses of a cross of German Landrace and Pietrain. Most of the changes in the liver of IUGR at midgestation involved various lipid metabolic pathways, both on transcript and metabolite levels, especially in the category of sphingolipids and phospholipids. Differentially expressed miRNAs, such as miR-34a, and their differentially expressed mRNA targets were identified. Sex-specific phenomena were observed at both the transcript and metabolite levels, particularly in male. This suggests that sex-specific adaptations in the metabolic system occur in the liver during midgestation (63 dpc). Our multi-omics network analysis reveals interactions and changes in the metabolic system associated with IUGR and identified an important biosignature that differs between IUGR and AGA piglets.
Subject(s)
Fetal Growth Retardation , MicroRNAs , Animals , Female , Fetal Growth Retardation/genetics , Fetal Growth Retardation/metabolism , Gestational Age , Humans , Liver/metabolism , Male , Metabolomics , Pregnancy , SwineABSTRACT
Legionella spp. cause Legionnaires' disease with pneumonia as the predominant clinical symptom. L. gormanii is the second most prevalent causative agent of community-acquired pneumonia after L. pneumophila. The study aimed to characterize the lipidome of L. gormanii membranes and the importance of these analyses in bacterial chemotaxonomy. Lipidomic analyses based on ultra-high performance liquid chromatography-mass spectrometry allowed the detection of individual molecular species of a wide range of L. gormanii membrane lipids contained in the outer (OM) and inner membranes (IM). The lipid profile comprised glycerolipids (triglycerides, diglycerides), phospholipids (phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, cardiolipin), and sphingolipids (ceramides, hexosylceramides). The most abundant lipid fraction in the IM and OM were phospholipids. The lipidomic analysis showed that two independent phosphatidylcholine (PC) synthesis pathways operating in L. gormanii: the PE-methylation (PmtA) pathway and the PC synthase (Pcs) pathway. Comparison of the molecular profile of PC species contained in the lipids of L. gormanii membranes cultured on the medium, with and without exogenous choline, showed quantitative differences in the PC pool. An unusual feature of the L. gormanii lipids was the presence of ceramides and hexosylceramides, which are typical components of eukaryotic cells and a very small group of bacteria. To the best of our knowledge, this is the first report of the occurrence of ceramides in Legionella bacteria.
ABSTRACT
Phospholipids (PL) are converted into lipid biomarkers by the action of phospholipases and reactive oxygen species (ROS), which are activated or released under certain physiological and pathophysiological conditions. Therefore, the in vivo concentration of such lipid biomarkers [e.g., lysophospholipids (LPLs)] is altered in humans and animals under different conditions such as inflammation, stress, medication, and nutrition. LPLs are particularly interesting because they are known to possess pro- and anti-inflammatory properties and may be generated by two different pathways: either by the influence of phospholipase A2 or by different reactive oxygen species that are generated in significant amounts under inflammatory conditions. Both lead to the cleavage of unsaturated acyl residues. This review provides a short summary of the mechanisms by which lipid biomarkers are generated under in vitro and in vivo conditions. The focus will be on lysophosphatidylcholine (LPC) because usually, this is the LPL species which occurs in the highest concentration and is, thus, easily detectable by chromatographic and spectroscopic methods. Finally, the effects of lipid biomarkers as signaling molecules and their roles in different human and animal pathologies such as infertility, cancer, atherosclerosis, and aging will be shortly discussed.
ABSTRACT
The analysis of the overall fatty acid pattern as well as their distribution in various lipid classes of phytopathogenic fungi Puccinia malvacearum and P. glechomatis are considered as chemotaxonomic biomarkers. Puccinia malvacearum on Alcea rosea and P. glechomatis on Glechoma hederacea collected from plants grown in various localities were analysed to determine their fatty acid composition. Both species synthesised significant amounts of saturated palmitic and stearic acids as well as 9,10-epoxy-octadecanoic acid, which rarely occurs in the nature. Both species synthesised hydroxy FAs including 9,10-dihydroxy octadecanoic acid and long-chain 2-hydroxy fatty acids.2-hydroxy 18:0 and 3-hydroxy 20:0 fatty acids were present only in P. malvacearum spores, and these may be the chemotaxonomic markers of the species. Ultra-high performance liquid chromatography mass spectrometry was performed for a comparative lipidomic analysis of P. malvacearum and P. glechomatis. The results revealed the complexity of molecular lipid species of these fungi. P. malvacearum and P. glechomatis lipids were characterised by the presence of a high number of triglyceride (TG) species. 9,10-epoxy octadecanoic fatty acid was found in TGs. Among the many types of oxidised TGs identified in P. glechomatis lipids, the most abundant species corresponds to TG(22:5+6O_17:0_18:2). P. malvacearum and P. glechomatis produced various ceramide species with different FAs from 14 to 24 chain-length. Unusual lipids like (O-acyl)-ω-hydroxy FA 18:0/18:0 in P. glechomatis and (O-acyl)-ω-hydroxy FA 18:0/20:0 and 18:0/22:0 in P. malvacearum were detected. The analysis of the polar lipid composition showed the presence of phosphatidylcholine and phosphatidylethanolamine as the main phospholipid classes of Puccinia spp. with the highest diversity of molecular species. Other phospholipids phosphatidic acid, phosphatidylglycerol phosphatidylserine and phosphatidylinositol were present in smaller amounts. The diversity of the neutral and polar lipid composition and fatty acid profile of P. malvacearum and P. glechomatis can be used in chemotaxonomic studies.
Subject(s)
Fatty Acids , Puccinia , Biomarkers , Fungi , PhospholipidsABSTRACT
Telmisartan prevents diet-induced obesity (DIO) in rodents. Given that the precise underlying mechanism is not known, we examined whether a gut-related mechanism might be involved. Sprague-Dawley rats received cafeteria diet (CD) for 3 months to develop DIO and were administered either telmisartan (8 mg/kgbw) or vehicle. In addition, pair-fed (PF) rats received CD adjusted to TEL and control rats (CON) only received chow. Stool samples were analysed by 16 S rRNA gene amplicon sequencing. CD-fed rats became obese while TEL, PF and CON rats remained lean. Alpha diversity analyses indicated that bacterial diversity was similar before the study but changed over time. Beta diversity revealed a time-, CD- and telmisartan-dependent effect. The Firmicutes/Bacteroidetes ratio and the abundance of Blautia, Allobaculum and Parasutterella were higher in DIO and PF than in CON, but not in TEL. Enterotype (ET)-like clustering analyses, Kleinberg's hub network scoring and random forest analyses also indicated that telmisartan induced a specific signature of gut microbiota. In response to stool transfer from telmisartan-pre-treated donor to high-fat fed acceptor mice, body weight gain was slightly attenuated. We attribute the anti-obesity action of telmisartan treatment to diet-independent alterations in gut microbiota as the microbiota from telmisartan-treated, CD-fed rats clearly differed from those of DIO and PF rats. ET-like clustering network, random forest classification and the higher stability in bacterial co-occurrence network analyses indicate that there is more than one indicator species for telmisartan's specific signature, which is further strengthened by the fact that we cannot identify a single indicator species.
Subject(s)
Angiotensin II Type 1 Receptor Blockers/pharmacology , Anti-Obesity Agents/pharmacology , Bacteria/drug effects , Gastrointestinal Microbiome/drug effects , Obesity/drug therapy , Telmisartan/pharmacology , Weight Gain/drug effects , Animals , Bacteria/growth & development , Diet/adverse effects , Disease Models, Animal , Dysbiosis , Fecal Microbiota Transplantation , Feces/microbiology , Mice , Obesity/etiology , Obesity/microbiology , Obesity/physiopathology , Rats , Rats, Sprague-DawleyABSTRACT
Several genetic variants in the mitochondrial genome (mtDNA), including ancient polymorphisms, are associated with chronic inflammatory conditions, but investigating the functional consequences of such mtDNA polymorphisms in humans is challenging due to the influence of many other polymorphisms in both mtDNA and the nuclear genome (nDNA). Here, using the conplastic mouse strain B6-mtFVB, we show that in mice, a maternally inherited natural mutation (m.7778G > T) in the mitochondrially encoded gene ATP synthase 8 (mt-Atp8) of complex V impacts on the cellular metabolic profile and effector functions of CD4+ T cells and induces mild changes in oxidative phosphorylation (OXPHOS) complex activities. These changes culminated in significantly lower disease susceptibility in two models of inflammatory skin disease. Our findings provide experimental evidence that a natural variation in mtDNA influences chronic inflammatory conditions through alterations in cellular metabolism and the systemic metabolic profile without causing major dysfunction in the OXPHOS system.
Subject(s)
DNA, Mitochondrial/genetics , Epidermolysis Bullosa Acquisita/genetics , Lymphocytes/metabolism , Polymorphism, Single Nucleotide , Animals , Cells, Cultured , Cytokines/metabolism , Epidermolysis Bullosa Acquisita/metabolism , Mice , Mice, Inbred C57BL , Mitochondria, Liver/genetics , Mitochondria, Liver/metabolism , Mitochondrial Proton-Translocating ATPases/geneticsABSTRACT
Lipids are involved in a broad spectrum of canonical biological functions, from energy supply and storage by triacylglycerols to membrane formation by sphingolipids, phospholipids and glycolipids. Because of this wide range of functions, there is an overlap between age-associated processes and lipid pathways. Lipidome analysis revealed age-related changes in the lipid composition of various tissues in mice and humans, which were also influenced by diet and gender. Some changes in the lipid profile can be linked to the onset of age-related neurodegenerative diseases like Alzheimer's disease. Furthermore, the excessive accumulation of lipid storage organelles, lipid droplets, has significant implications for the development of inflammaging and non-communicable age-related diseases. Dietary interventions such as caloric restriction, time-restrictive eating, and lipid supplementation have been shown to improve pertinent health metrics or even extend life span and thus modulate aging processes.
ABSTRACT
Legionella species synthesize phosphatidylcholine (PC) in two independent pathways: the three-step methylation of phosphatidylethanolamine PMT pathway and the one-step PCS pathway, in which the Pcs enzyme catalyzes the reaction between choline and CDP-diacylglycerol to form PC. Legionella pcs genes encode highly hydrophobic proteins with phosphatidylcholine synthase activity, which contain up to eight transmembrane helices with N- and C-termini located inside the bacterial cell. The comparative analysis of nucleotide sequences of pcs showed that these genes share high sequence identity among members of the Legionellaceae family. Legionella pmtA genes involved in the PMT pathway encoded small cytosolic proteins with putative phosphatidylethanolamine N-methyltransferase activity. The pmtA genes identified in Legionella species had lower sequence identity to each other than the pcs genes. The phylogenetic tree constructed based on the pcs and pmtA gene sequences showed phylogenetic relatedness between Legionella spp. and other bacteria. The utilization of extracellular choline by the four Legionella species leads to changes not only in the lipid components but also in proteins, and the interactions between these components lead to changes in cell surface properties, which result in a decline in induction of proinflammatory cytokines (TNF-α and IL-6).
Subject(s)
Aminoacyltransferases/genetics , Bacterial Proteins/genetics , Choline/metabolism , Cytokines/metabolism , Inflammation Mediators/metabolism , Legionella/genetics , Legionellosis/metabolism , Legionellosis/microbiology , Methyltransferases/genetics , Genes, Bacterial , Genetic Variation , Humans , Legionella/chemistry , Legionella/classification , Lipid Metabolism , Lipids/chemistry , Magnetic Resonance Spectroscopy , Phospholipids/chemistry , Phospholipids/metabolism , Phylogeny , Spectroscopy, Fourier Transform InfraredABSTRACT
Fat is historically associated with poor health and obesity. However, the continuous use of lipidomics and genetic studies in model organisms revealed that specific lipid profiles and signals might delay aging. In order to identify and quantify the lipid species, researchers are taking advantage of the recent developments in the area of lipidomics that is mainly done by mass spectrometry and further techniques, such as NMR spectroscopy and chromatographic separations. This review will emphasize the role of lipid composition and metabolism during aging. We review the molecular and physiological changes during the progression of aging with a special focus on the role of lipids. Interventions to modulate life span in a variety of organisms such caloric restriction, show a significant extension of their maximum life-span and a decrease in the onset of age-related diseases. In particular, the influence of dietary restriction in lipid metabolism will be a major point of this review.
Subject(s)
Aging , Lipids/chemistry , Animals , Humans , Lipid MetabolismABSTRACT
Horse serum is commonly used as an additive to support the maintenance of hematopoietic progenitor cells in culture. However, the wide variability in the performance of different lots calls for parallel testing of multiple batches over extended periods of culture. Identification of the serum components that determine hematopoietic support would therefore save considerable time and effort and would help to standardize culture procedures. We report here that the ability of horse serum to support the self-renewal of multipotent murine hematopoietic progenitor FDCP-Mix cells is correlated to the concentration of specific fatty acid products of phospholipase A2 and more closely to the spectrum of eicosanoids generated by their further processing through cyclooxygenase and lipoxygenase pathways. Supportive sera have low levels of lysophosphatidylcholine and inflammatory eicosanoids. This links known markers of inflammation, infection and platelet activation to the ability of serum to maintain progenitor cells in an undifferentiated state, providing a means for prospective identification of suitable sera as well as quality control of the production process.
Subject(s)
Hematopoiesis , Hematopoietic Stem Cells/drug effects , Phospholipases A2/analysis , Serum/chemistry , Animals , Eicosanoids/analysis , Eicosanoids/pharmacology , Hematopoietic Stem Cells/cytology , Horses , Lipids/analysis , Lipids/pharmacology , Lipoxygenase/metabolism , Lysophosphatidylcholines/analysis , Lysophosphatidylcholines/pharmacology , Mass Spectrometry , Mice , Phospholipases A2/metabolism , Prostaglandin-Endoperoxide Synthases/metabolism , Serum/metabolismABSTRACT
Lysophosphatidylcholine (lysoPtdCho) is a well-known biomarker in body fluids for inflammation and oxidative stress and provides a possible clinical screening marker for certain diseases where inflammation is involved. It was shown in our previous article that the measurement of intact serum using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) provides the phosphatidylcholine (PtdCho)/lysoPtdCho ratios faster than the measurements after organic extraction, while the standard deviations of those "intact" measurements are even smaller. Surprisingly, the PtdCho/lysoPtdCho ratio is about two times higher in the intact serum MALDI-TOF MS measurement than in the MALDI-TOF MS analysis of the organic extracts. Albumin binding of lysoPtdCho seems to be a very likely reason for increased PtdCho/lysoPtdCho ratios in the intact serum measurements. In this article, this hypothesis is tested on horse serum as a biological sample. Albumin (equine and bovine) addition to serum shows an increase in the PtdCho/lysoPtdCho ratio detected by MALDI-TOF MS. Further experiments with a comparable lipid model suspension verify that pepsin and trypsin are able to liberate the bound lipids. Under different conditions, the effects of both enzymes on the lipid model suspension are compared. Finally, an improved MALDI-TOF MS measurement of the PtdCho/lysoPtdCho ratio in intact serum after a prior pepsin digestion step was established. As is known that lysoPtdCho is cytotoxic and albumin is capable of decreasing this cytotoxicity by binding lysoPtdCho, this study proposes to consider both PtdCho/lysoPtdCho ratios-with and without albumin-bound lysoPtdCho-that could be superior diagnostic markers for inflammation and oxidative stress.
Subject(s)
Lysophosphatidylcholines/blood , Phosphatidylcholines/blood , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Animals , Cattle , Horses , Humans , Proteolysis , Serum Albumin/analysisABSTRACT
BACKGROUND: Reduced erythrocyte survival and deformability may contribute to the so-called anemia of inflammation observed in septic patients. Erythrocyte structure and function are affected by both the membrane lipid composition and the organization. We therefore aimed to determine whether these parameters are affected during systemic inflammation. METHODS: A sensitive matrix-assisted laser desorption and ionization time-of-flight mass spectrometric method was used to investigate the effect of plasma components of 10 patients with septic shock and of 10 healthy volunteers subjected to experimental endotoxemia on erythrocyte membrane lipid composition. RESULTS: Incubation of erythrocytes from healthy control donors with plasma from patients with septic shock resulted in membrane phosphatidylcholine hydrolysis into lysophosphatidylcholine (LPC). Plasma from volunteers undergoing experimental human endotoxemia did not induce LPC formation. The secretory phospholipase A2 IIA concentration was enhanced up to 200-fold in plasma of septic patients and plasma from endotoxin-treated subjects, but did not correlate with the ability of these plasmas to generate LPC. Erythrocyte phosphatidylserine exposure increased up to two-fold during experimental endotoxemia. CONCLUSIONS: Erythrocyte membrane lipid remodeling as reflected by LPC formation and/or PS exposure occurs during systemic inflammation in a secretory phospholipase A2 IIA-independent manner. GENERAL SIGNIFICANCE: Sepsis-associated inflammation induces a lipid remodeling of the erythrocyte membrane that is likely to affect erythrocyte function and survival, and that is not fully mimicked by experimental endotoxemia.
ABSTRACT
Reactive oxygen species (ROS) play important physiological roles and are of particular relevance in the pathogenesis of inflammatory diseases. At inflammatory conditions, the enzyme myeloperoxidase generates hypochlorous acid (HOCl) which adds to the double bonds of fatty acyl residues of (phospho)lipids under the formation of chlorohydrins. This may lead to the development of many inflammatory diseases, such as atherosclerosis or arthritis, if the ROS generation exceeds a certain extent. Using oleic acid as the simplest unsaturated fatty acid which contains just a single double bond, as a model system, we investigated all products - including the chlorohydrin - after its reaction with HOCl by a combination of thin-layer chromatography and electrospray ionization mass spectrometry. Unlike the general acceptance, the reaction of oleic acid and HOCl leads not exclusively to the formation of chlorohydrin (isomers) but is much more complex: there are also considerable amounts of dimeric and (to a minor extent) trimeric products which can be assigned to isomeric ethers and esters. The obtained products after oleic acid chlorination were also compared with the reaction products of 1-palmitoyl-2-oleoyl-sn-phosphatidylcholine (POPC) and HOCl. The reasons why different products are obtained will be discussed and the involvement of the carboxylic acid emphasized.
Subject(s)
Hypochlorous Acid/chemistry , Oleic Acid/chemistry , Chlorohydrins/chemistry , Chromatography, Thin Layer , Dimerization , Esters , Halogenation , Isomerism , Oxidation-Reduction , Phosphatidylcholines/chemistry , Spectrometry, Mass, Electrospray IonizationABSTRACT
The PC/LPC ratio of blood serum is increasingly considered to represent an important clinical parameter that reflects various kinds of diseases. Here, a simple and fast method of lipid analyses of "intact" blood serum (i.e. without extraction) by MALDI-TOF mass spectrometry is described. The novel procedure allows the accurate determination of the PC/LPC ratio, utilizing only a tiny amount of blood. The serum is diluted with distilled water and directly applied onto the MALDI target and, after drying, covered by a thin layer of the matrix solution (either 9-aminoacridine or 2,5-dihydroxybenzoic acid). Positive ion mass spectra acquired by using this procedure give similar peak patterns as the spectra of the lipid extracts of horse blood serum. Blood serum from fourteen different horses was used to set up and validate the new method of lipid analysis. The PC/LPC ratios determined with the fast "intact" method were compared with those obtained with classical MALDI-TOF MS and (31)P NMR analyses of the corresponding lipid extracts. As comparable data were obtained, this is a clear indication that extraction is not an absolute necessity.
Subject(s)
Inflammation/blood , Lysophosphatidylcholines/blood , Phosphatidylcholines/blood , Spectrometry, Mass, Electrospray Ionization/methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Animals , Biomarkers/blood , Horses , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
Acanthamoeba are opportunistic protozoan pathogens that may lead to sight-threatening keratitis and fatal granulomatous encephalitis. The successful prognosis requires early diagnosis and differentiation of pathogenic Acanthamoeba followed by aggressive treatment regimen. The plasma membrane of Acanthamoeba consists of 25% phospholipids (PL). The presence of C20 and, recently reported, 28- and 30-carbon fatty acyl residues is characteristic of amoeba PL. A detailed knowledge about this unusual PL composition could help to differentiate Acanthamoeba from other parasites, e.g. bacteria and develop more efficient treatment strategies. Therefore, the detailed PL composition of Acanthamoeba castellanii was investigated by 31P nuclear magnetic resonance spectroscopy, thin-layer chromatography, gas chromatography, high performance liquid chromatography and liquid chromatography-mass spectrometry. Normal and reversed phase liquid chromatography coupled with mass spectrometric detection was used for detailed characterization of the fatty acyl composition of each detected PL. The most abundant fatty acyl residues in each PL class were octadecanoyl (18â¶0), octadecenoyl (18â¶1 Δ9) and hexadecanoyl (16â¶0). However, some selected PLs contained also very long fatty acyl chains: the presence of 28- and 30-carbon fatty acyl residues was confirmed in phosphatidylethanolamine (PE), phosphatidylserine, phosphatidic acid and cardiolipin. The majority of these fatty acyl residues were also identified in PE that resulted in the following composition: 28â¶1/20â¶2, 30â¶2/18â¶1, 28â¶0/20â¶2, 30â¶2/20â¶4 and 30â¶3/20â¶3. The PL of amoebae are significantly different in comparison to other cells: we describe here for the first time unusual, very long chain fatty acids with Δ5-unsaturation (30â¶35,21,24) and 30â¶221,24 localized exclusively in specific phospholipid classes of A. castellanii protozoa that could serve as specific biomarkers for the presence of these microorganisms.
Subject(s)
Acanthamoeba castellanii/metabolism , Phospholipids/metabolism , Amebiasis/diagnosis , Amebiasis/parasitology , Biomarkers/metabolism , Humans , Magnetic Resonance Spectroscopy , Molecular Structure , Phospholipids/chemistry , Phospholipids/isolation & purificationABSTRACT
Normal phase thin-layer chromatography (NP TLC) is an established method of (phospho)lipid analysis. The determination of the fatty acyl composition is, however, a more challenging task by NP TLC. The direct coupling of TLC separation with mass spectrometric detection (e.g., matrix-assisted laser desorption/ionization mass spectrometry, MALDI MS), however, enables a detailed characterization of complex lipid mixtures. Here we show that the thickness of the silica gel layer has a considerable effect on the quality of the mass spectra recorded directly from the TLC plate. In particular, the intensity of the matrix background signals can be reduced if "thinner" TLC layers are used.
Subject(s)
Chromatography, Thin Layer , Lipids/analysis , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Phosphatidylcholines/analysis , Phosphatidylethanolamines/analysis , Silica Gel/chemistryABSTRACT
SIGNIFICANCE: Inflammatory diseases (such as arthritis) of the extracellular matrix (ECM) are of considerable socioeconomic significance. There is clear evidence that reactive oxygen species (ROS) and nitrogen species released by, for instance, neutrophils contribute to the degradation of the ECM. Here we will focus on the ROS-induced degradation of the glycosaminoglycans, one important component of the ECM. RECENT ADVANCES: The recently developed "anti-TNF-α" therapy is primarily directed against neutrophilic granulocytes that are powerful sources of ROS. Therefore, a more detailed look into the mechanisms of the reactions of these ROS is reasonable. CRITICAL ISSUES: Since both enzymes and ROS contribute to the pathogenesis of inflammatory diseases, it is very difficult to estimate the contributions of the individual species in a complex biological environment. This particularly applies as many products are not stable but only transient products that decompose in a time-dependent manner. Thus, the development of suitable analytical methods as well as the establishment of useful biomarkers is a challenging aspect. FUTURE DIRECTIONS: If the mechanisms of ECM destruction are understood in more detail, then the development of suitable drugs to treat inflammatory diseases will be hopefully much more successful.