ABSTRACT
Bovine tuberculosis (BTB) is a zoonotic disease caused by Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex (MTC). The quick and specific detection of this species is of extreme importance, since BTB may cause economic impacts, in addition to presenting imminent risks to human health. In the present study a nested real-time PCR test (nested q-PCR) was used in post-mortem evaluations to assess cattle carcasses with BTB-suspected lesions. A total of 41,193 cattle slaughtered in slaughterhouses located in the state of Mato Grosso, were examined. Of the examined animals, 198 (0.48%) showed BTB-suspected lesions. M. bovis was isolated in 1.5% (3/198) of the samples. Multiplex-PCR detected MTC in 7% (14/198) of the samples. The nested q-PCR test detected MTC in 28% (56/198) of the BTB-suspected lesions, demonstrating higher efficiency when compared to the multiplex-PCR and conventional microbiology. Nested q-PCR can therefore be used as a complementary test in the national program for control and eradication of bovine tuberculosis.
Subject(s)
DNA, Bacterial/analysis , Food Inspection/methods , Meat/microbiology , Molecular Typing/veterinary , Mycobacterium bovis/isolation & purification , Tuberculosis, Bovine/microbiology , Tuberculosis, Lymph Node/veterinary , Abattoirs , Animals , Brazil , Cattle , DNA, Bacterial/isolation & purification , DNA, Bacterial/metabolism , Efficiency , Head , Limit of Detection , Lung/chemistry , Lung/microbiology , Lymph Nodes/chemistry , Lymph Nodes/microbiology , Meat/analysis , Molecular Typing/methods , Multiplex Polymerase Chain Reaction/veterinary , Mycobacterium bovis/classification , Neck , Real-Time Polymerase Chain Reaction/veterinary , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Thorax , Tuberculosis, Bovine/physiopathology , Tuberculosis, Bovine/prevention & control , Tuberculosis, Lymph Node/etiologyABSTRACT
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
Colônias isoladas a partir de lesões sugestivas de tuberculose bovina foram testadas pela reação múltipla em cadeia da polimerase, usando oligonucleotídeos direcionados para as seqüências genômicas RvD1Rv2031c e IS6110, específicas para M. bovis e para o complexo Mycobacterium tuberculosis, respectivamente. A m-PCR identificou, com sucesso, 88,24% das colônias isoladas como M. bovis.
Subject(s)
Animals , Cattle , Base Sequence , In Vitro Techniques , Mycobacterium bovis/isolation & purification , Oligonucleotides/analysis , Polymerase Chain Reaction , Tuberculosis, Bovine , Methods , MethodsABSTRACT
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
