Predator traits determine food-web architecture across ecosystems.

Predator-prey interactions in natural ecosystems generate complex food webs that have a simple universal body-size architecture where predators are systematically larger than their prey. Food-web theory shows that the highest predator-prey body-mass ratios found in natural food webs may be especially important because they create weak interactions with slow dynamics that stabilize communities against perturbations and maintain ecosystem functioning. Identifying these vital interactions in real communities typically requires arduous identification of interactions in complex food webs. Here, we overcome this obstacle by developing predator-trait models to predict average body-mass ratios based on a database comprising 290 food webs from freshwater, marine and terrestrial ecosystems across all continents. We analysed how species traits constrain body-size architecture by changing the slope of the predator-prey body-mass scaling. Across ecosystems, we found high body-mass ratios for predator groups with specific trait combinations including (1) small vertebrates and (2) large swimming or flying predators. Including the metabolic and movement types of predators increased the accuracy of predicting which species are engaged in high body-mass ratio interactions. We demonstrate that species traits explain striking patterns in the body-size architecture of natural food webs that underpin the stability and functioning of ecosystems, paving the way for community-level management of the most complex natural ecosystems.

Comparing biotic drivers of litter breakdown across stream compartments.

Litter breakdown in the streambed is an important pathway in organic carbon cycling and energy transfer in the biosphere that is mediated by a wide range of streambed organisms. However, most research on litter breakdown to date has focused on a small fraction of the taxa that drive it (e.g. microbial vs. macroinvertebrate-mediated breakdown) and has been limited to the benthic zone (BZ). Despite the importance of the hyporheic zone (HZ) as a bioreactor, little is known about what, or who, mediates litter breakdown in this compartment and whether breakdown rates differ between the BZ and HZ. Here, we explore the relationship between litter breakdown and the variation in community structure of benthic and hyporheic communities by deploying two standardized bioassays (cotton strips and two types of commercially available tea bags) in 30 UK streams that encompass a range of environmental conditions. Then, we modelled these assays as a response of the streambed compartment and the biological features of the streambed assemblage (Prokaryota, Protozoa and Eumetazoa invertebrates) to understand the generality and efficiency of litter processing across communities. Litter breakdown was much faster in the BZ compared with the HZ (around 5 times higher for cotton strips and 1.5 times faster for the tea leaves). However, differences in litter breakdown between the BZ and the HZ were mediated by the biological features of the benthos and the hyporheos. Biomass of all the studied biotic groups, α-diversity of Eumetazoa invertebrates and metabolic diversity of Prokaryota were important predictors that were positively related to breakdown coefficients demonstrating their importance in the functioning of the streambed ecosystem. Our study uses a novel multimetric bioassay that is able to disentangle the contribution by Prokaryota, Protozoa and Eumetazoa invertebrates to litter breakdown. In doing so, our study reveals new insights into how organic matter decomposition is partitioned across biota and streambed compartments.

Chronic effects of brine discharge form large-scale seawater reverse osmosis desalination facilities on benthic bacteria.

Seawater desalination facilities continuously discharge hyper-saline brine into the coastal environment which often flows as a concentrated plume over the seafloor, hence possibly impacting benthic microorganisms. Yet, the effects of brine discharge from desalination plants on benthic bacteria, key players in biodegradation of organic material and nutrient recycling is unknown. In this study, we tested the chronic (years) effects of brine discharge from three large-scale desalination facilities on the abundance, metabolic activity and community composition of benthic bacteria. To this end, four sampling campaigns were carried at the outfall areas of the Ashkelon, Sorek and Hadera desalination facilities. The effects of the brine were compared to corresponding reference stations which were not influenced by the brine (i.e., water temperature and salinity). Our sampling data indicate that bacterial abundance and activity that includes bacterial growth efficiency were 1.3-2.6-fold higher at the outfall area than the reference station. Concomitant analysis pointed out that the bacterial community structure at the brine discharge area was also different than the reference station, yet varied between each desalination facility. Our results demonstrate that the impact of brine effluent from desalination facilities on benthic bacteria are site-specific and localized (<1.4 Km2) around the discharge point. Namely, that the effects on benthic bacteria are prominent at the brine mixing zone and change according to the discharge method used to disperse the brine as well as local stressors (e.g., eutrophication and elevated water temperature). Our results contribute new insights on the effects of desalination-brine to benthic microbes, while providing scientifically-based aspects on the ecological impacts of brine dispersion for decision makers.