Optical power profiles and aberrations of a non-diffractive wavefront-shaping extended depth of focus intraocular lens.

PURPOSE: This study is to evaluate the optical characteristics of a non-diffractive wavefront-shaping intraocular lens which incorporates surface refractive modifications for shaping the wavefront in order to achieve extended depth of focus (EDoF) and to assess whether the nominal power of this IOL influences the attainable add power. METHODS: A commercially available optical bench NIMO TR1504 device (LAMBDA-X, Nivelles, Belgium) was employed to obtain full optical characterization of three non-diffractive EDoF intraocular lenses with + 10 D, + 20 D, and + 30 D powers. After NIMO measurements, data were computed using a custom-made MATLAB program (Mathworks, Inc., Natick, MA, USA) to evaluate the optical quality functions, such as the point spread function (PSF), wavefront profiles, and modulation transfer function (MTF) for two pupil sizes: 3 mm and 4.0 mm. RESULTS: The non-diffractive EDoF intraocular lens showed a central serrated power profile behavior with additions of + 2.00 to + 2.50 D over the nominal power. Higher order aberrations were found to be driven mainly by the spherical aberration, with almost null comatic influence. Optical quality metrics showed good values, better for a 3 mm pupil compared to a 4.5 mm one, as expected. The three IOL powers tested showed a very similar behavior in terms of power and aberrometric profiles, with minimal to null differences related to the nominal power. CONCLUSION: The non-diffractive wavefront-shaping EDoF intraocular lens achieves a near addition up to + 2.50 D aiming for an extended range of vision, almost independently of the base power.

Optical and Clinical Outcomes of an Isofocal Intraocular Lens vs. a Monofocal Standard Lens.

The aim of this study is to evaluate the results obtained on the optical bench and clinically with an isofocal lens (ISOPure, BVI medical, Belgium) to compare them to a standard monofocal one (MicroPure, BVI medical, Belgium). To do so, we have combined laboratory investigation and a prospective, comparative, and randomized clinical study. First, we have measured the wavefront of the two models studied using a NIMO TR1504 (Lambda-X, Belgium) deflectometer for three nominal powers: +10.00, +20.00 and +30.00 D. In the randomized study with 48 patients, half of them implanted with ISOPure and the other with MicroPure, we have measured visual acuities and contrast sensitivity under photopic and mesopic conditions. The optical bench results show that the isofocal lens presented higher power than the monofocal one, at the lens center, due to the spherical aberration (coefficients Z(4,0), Z(6,0) and Z(8,0)) induced by the greater asphericity of its design. The addition obtained depended on the nominal power, from +1.00 to +1.50 D. The results of the clinical study showed that the ISOPure lens presented better visual outcomes, which were statistically significant, at intermediate distance compared to the MicroPure lens (p-values of 0.014 and 0.022 for 80 and 60 cm, respectively) without decreasing the contrast sensitivity. Clinical outcomes were not affected by pupillary size. In conclusion, due to the increase in power at the lens center due to its highly aspherical design, the isofocal lens evaluated showed better intermediate vision than the monofocal one.

Real-time correction of chromatic aberration in optical fluorescence microscopy.

Multi-color fluorescence imaging is a powerful tool for studying the spatial relationships and interactions among sub-cellular structures in biological specimens. However, if improperly corrected, geometrical distortions caused by mechanical drift, refractive index mismatch, or chromatic aberration can lead to lower image resolution. In this paper, we present an extension of the image processing framework of Scipion by integrating a protocol called OFM Corrector, which corrects geometrical distortions in real-time using a B-spline-based elastic continuous registration technique. Our proposal provides a simple strategy to overcome chromatic aberration by digitally re-aligning color channels in multi-color fluorescence microscopy images, even in 3D or time. Our method relies on a geometrical calibration, which we do with fluorescent beads excited by different wavelengths of light and subsequently registered to get the elastic warp as a reference to correct chromatic shift. Our software is freely available with a user-friendly GUI and can be broadly used for various biological imaging problems. The paper presents a valuable tool for researchers working in light microscopy facilities.

Correlation between reading time and characteristics of eye fixations and progressive lens design.

OBJECTIVE: The purpose of this study is to evaluate reading time and characteristics of fixations at different distances when looking through different areas of progressive power lenses (PPL) with different power distributions by means of eye-tracking technology. METHOD: A wearable eye tracker system (Tobii-Pro Glasses 3) was used to record the pupil position of 28 PPL subjects when reading at near and distance vision while using 3 different PPL designs: a PPL optimized for distance vision (PPL-Distance), a PPL optimized for near vision (PPL-Near) and one of them balanced for a general use (PPL-Balance). Subjects were asked to read out loud a text displayed on a digital screen located at 5.25m and 0.37m when they were looking through the central and peripheral regions of each PPL. Reading time, total duration of fixations, and the number of fixations were analyzed for each reading condition and PPL. Statistical analysis was carried out using Statgraphics Centurion XVII.II Software. RESULTS: The analysis of eye movements at distance-reading vision showed a statistically significant lower reading time (p = 0.004) and lower total duration of fixations (p = 0.01) for PPL-Distance. At near-reading vision, PPL-Near provided statistically significant lower reading time (p<0.001), lower total duration of fixations (p = 0.02), and less fixation count(p<0.001) in comparison with PPL-Balance and PPL-Distance. CONCLUSIONS: Reading time and fixations characteristics are affected by the power distribution of a PPL. A PPL design with a wider distance region provides better distance-reading performance while a PPL with a wider near area performs better at a near-reading task. The power distribution of PPLs influences the user performance at vision-based tasks. Thus, to provide the user with the best visual experience, PPL selection must consider user needs.

TrackAnalyzer: A Fiji/ImageJ toolbox for a holistic analysis of tracks.

Current live-cell imaging techniques make possible the observation of live events and the acquisition of large datasets to characterize the different parameters of the visualized events. They provide new insights into the dynamics of biological processes with unprecedented spatial and temporal resolutions. Here we describe the implementation and application of a new tool called TrackAnalyzer, accessible from Fiji and ImageJ. Our tool allows running semi-automated single-particle tracking (SPT) and subsequent motion classification, as well as quantitative analysis of diffusion and intensity for selected tracks relying on the graphical user interface (GUI) for large sets of temporal images (X-Y-T or X-Y-C-T dimensions). TrackAnalyzer also allows 3D visualization of the results as overlays of either spots, cells or end-tracks over time, along with corresponding feature extraction and further classification according to user criteria. Our analysis workflow automates the following steps: (1) spot or cell detection and filtering, (2) construction of tracks, (3) track classification and analysis (diffusion and chemotaxis), and (4) detailed analysis and visualization of all the outputs along the pipeline. All these analyses are automated and can be run in batch mode for a set of similar acquisitions.

Eye movements as a predictor of preference for progressive power lenses.

The purpose of this study is to determine if there is any correlation between the characteristics of the user's eye movements (EMs) and the preference of the user when wearing different Progressive power lenses (PPLs) distributions. An eye-tracker system with a sample rate of 120Hz and temporal resolution of 8.3ms (Tobii-X3-120) was used to register EMs of 38 PPL users when reading in a computer screen with 2 types of PPLs (PPLsoft and PPL-hard). Number of fixations, complete fixation time, fixation duration mean, saccade duration mean, saccade distance mean, and number of regressions were analyzed for 6 different regions of the computer screen. A statistically significant difference was observed between the characteristics of the user's EMs and the user's PPL subjective preference (p < 0.05*). Subjects that preferred the PPL-hard presented significantly lower complete fixation time, lower fixation duration mean and lower number of regressions than those subjects indicating a preference for the PPL-soft. Results of this study suggest that eye-tracking systems can be used as PPL design recommendation systems according to the user EMs performance.

Numerical model of the inhomogeneous scattering by the human lens.

We present in this work a numerical model for characterizing the scattering properties of the human lens. After analyzing the scattering properties of two main scattering particles actually described in the literature through FEM (finite element method) simulations, we have modified a Monte Carlo's bulk scattering algorithm for computing ray scattering in non-sequential ray tracing. We have implemented this ray scattering algorithm in a layered model of the human lens in order to calculate the scattering properties of the whole lens. We have tested our algorithm by simulating the classic experiment carried out by Van der Berg et al for measuring "in vitro" the angular distribution of forward scattered light by the human lens. The results show the ability of our model to simulate accurately the scattering properties of the human lens.

Modulation transfer function for infrared reflectarrays.

The quality of the image produced by optical reflectarrays as a function of the F/#, polarization, and wavelength is analyzed in this paper. The results are expressed as monochromatic and polychromatic modulation transfer functions. They show that large aperture multilevel reflectarrays perform quite close to the diffraction-limited case. The chromatic aberrations make these elements highly wavelength-selective.

Monochromatic aberrations in resonant optical elements applied to a focusing multilevel reflectarray.

The monochromatic aberrations produced by the phase distribution reflected by resonant sub-wavelength metallic structures are studied both analytically and numerically. Even for normal incidence, the angular dependence of the re-radiated wavefront disturbs the overall performance of the reflectarray. This effect is modelled as combination of a linear and a cubic dependence. A complete numerical simulation of a multilevel focusing reflectarray is performed using computational-electromagnetic and physical-optics-propagation methods. A modified Strehl ratio is defined to show the dependence of the focused spot behavior on aperture. The irradiance distribution is dependent on the polarization state. A small-aperture focusing reflectarray has been designed, fabricated, and tested. The irradiance distribution at the focusing plane is compared with the simulated one, showing a good agreement when residual wavefront aberrations are included.

Steerable spatial phase shifting applied to single-image closed-fringe interferograms.

It is well known that spatial phase shifting interferometry (SPSI) may be used to demodulate two-dimensional (2D) spatial-carrier interferograms. In these cases the application of SPSI is straightforward because the modulating phase is a monotonic increasing function of space. However, this is not true when we apply SPSI to demodulate a single-image interferogram containing closed fringes. This is because using these algorithms, one would obtain a wrongly demodulated monotonic phase all over the 2D space. We present a technique to overcome this drawback and to allow any SPSI algorithm to be used as a single-image fringe pattern demodulator containing closed fringes. We make use of the 2D spatial orientation direction of the fringes to steer (orient) the one-dimensional SPSI algorithm in order to correctly demodulate the nonmonotonic 2D phase all over the interferogram.

Design of asynchronous phase detection algorithms optimized for wide frequency response.

In many fringe pattern processing applications the local phase has to be obtained from a sinusoidal irradiance signal with unknown local frequency. This process is called asynchronous phase demodulation. Existing algorithms for asynchronous phase detection, or asynchronous algorithms, have been designed to yield no algebraic error in the recovered value of the phase for any signal frequency. However, each asynchronous algorithm has a characteristic frequency response curve. Existing asynchronous algorithms present a range of frequencies with low response, reaching zero for particular values of the signal frequency. For real noisy signals, low response implies a low signal-to-noise ratio in the recovered phase and therefore unreliable results. We present a new Fourier-based methodology for designing asynchronous algorithms with any user-defined frequency response curve and known limit of algebraic error. We show how asynchronous algorithms designed with this method can have better properties for real conditions of noise and signal frequency variation.

Fast algorithm for estimation of the orientation term of a general quadrature transform with application to demodulation of an n-dimensional fringe pattern.

The spatial orientation of fringes has been demonstrated to be a key point in reliable phase demodulation from a single n-dimensional fringe pattern, regardless of the frequency spectrum of the signal. Recent publications have shown a general method for determination of the orientation factor by use of a regularized phase-tracking (RPT) algorithm. We propose a generalization of a RPT algorithm for estimation of the spatial orientation in a general n-dimensional case. The proposed algorithm makes use of a simplified cost function that remains one dimensional regardless of the dimension of the problem. This makes the calculation faster than with a standard RPT algorithm, with which it is necessary to minimize an n + 1-dimensional cost function for each point of the sample space. We have applied the method to the three-dimensional demodulation of a time-evolving fringe pattern, with good results.

Improved method for isochromatic demodulation by RGB calibration.

The red-blue-green (RGB) calibration technique consists in constructing an a priori calibration table of the isochromatic retardation versus the triplet of RGB values obtained with a RGB CCD camera. In this way a lookup table (LUT) is built in which the entry is the corresponding RGB triplet and the output is the given retardation. This calibration (a radiometric quantity) depends on the geometric and chromatic parameters of the setup. Once the calibration is performed, the isochromatic retardation at a given point of the sample is computed as the one that minimizes the Euclidean distance between the measured RGB triplet and the triplets stored in the LUT. We present an enhanced RGB calibration algorithm for isochromatic fringe pattern demodulation. We have improved the standard demodulation algorithm used in RGB calibration by changing the Euclidean cost function to a regularized one in which the fidelity term corresponds to the Euclidean distance between RGB triplets; the regularizing term forces piecewise continuity for the isochromatic retardation. Additionally we have implemented a selective search in the RGB calibration LUT. We have tested the algorithm with simulated as well as real photoelastic data with good results.