ABSTRACT
The present study described an extremely rare case of acute promyelocytic leukemia (APL) characterized by a complex threeway (15;22;17)(q22;q11.2;q21) translocation. Acute promyelocytic leukemia (APL) is a specific subtype of acute myeloid leukemia with distinctive clinical and therapeutic characteristics. Besides being characterized by the t(15;17)(q22;q12) translocation, this subtype is also notable for its response to all-trans-retinoic acid (ATRA) treatment. APL is highly responsive to a combination of ATRA and chemotherapeutic agents, achieving over 90 % complete remission rates and over 80 % long-term remission rates. In this case, a 79-year-old male patient presented with complaints of weakness, fatigue, and petechial rash, with no other significant medical history except for diabetes mellitus and hypertension. Conventional cytogenetic methods, dual-color dual-fusion, and dual-color break-apart fluorescent in situ hybridization techniques together identified the t(15;22;17) translocation. RT-PCR analysis was performed for expression of PML/RARA fusion transcripts. The patient, diagnosed with APL, exhibited a complete response to all-trans retinoic acid (ATRA) and idarubicin treatment. In this paper, we present the second documented case of t(15;22;17) and explore the remarkable remission observed following treatment with All-Trans Retinoic Acid (ATRA).
Subject(s)
Chromosomes, Human, Pair 17 , Leukemia, Promyelocytic, Acute , Translocation, Genetic , Humans , Male , Leukemia, Promyelocytic, Acute/genetics , Leukemia, Promyelocytic, Acute/drug therapy , Aged , Chromosomes, Human, Pair 17/genetics , Tretinoin/therapeutic use , Chromosomes, Human, Pair 15/genetics , Chromosomes, Human, Pair 22/genetics , In Situ Hybridization, FluorescenceABSTRACT
The biguanide Cu(II) complexes [Cu(L1-4)2](ClO4)2 were synthesized and spectroscopic/analytical techniques were used to clarify their structures. Single crystals of complex [Cu(L4)2](ClO4)2 was obtained and its definite structure was determined by single crystal X-ray diffraction study. The complexes [Cu(L1-4)2](ClO4)2 were screened for their FSds-DNA interactions by using UV-Vis absorption and fluorescence spectroscopies. The complexes [Cu(L2)2](ClO4)2 and [Cu(L3)2](ClO4)2 were shown to exhibit higher affinity for binding DNA. Examining the EB-DNA interaction, it is believed that the complexes interact with DNA through a groove binding mechanism. The interaction of complexes with BSA were observed through the quenching of the fluorescence emission. Complexes [Cu(L2)2](ClO4)2 and [Cu(L3)2](ClO4)2 show moderate binding affinity to BSA through a static mode. Additionally, the Cu(II) complexes were screened for their antibacterial activities against various bacterial strains. Complexes showed potential antimicrobial activity against Salmonella typhimurium, Bacillus cereus, Salmonella typhimurium and S. aureus.
ABSTRACT
The aim of the study was to evaluate the severity of molar incisor hypomineralisation (MIH), related oral health and investigate salivary mineral composition. The study was conducted with 50 participants aged between 6-15 years who were effected with MIH and 50 without MIH. The International Caries Detection and Assessment System (ICDAS) scores, Decayed, Missing, Filled Teeth/Surface (DMFT/S), dft/s and gingival/plaque indices were evaluated. The pH, flow rate, buffering capacity and mineral composition of saliva was measured. "Student t" test, one-way analysis of variance in repeated measurements of groups, and Tukey multiplex in subgroup comparisons was used. Kruskal-Wallis, Mann-Whitney U, Wilcoxon and chi-square tests were used to analyze qualitative data and compare groups. A total of 100 children (57 females 43 males, mean age 10.12 ± 1.85) participated in the study. There was no difference between ICDAS, DMFT/S scores, but dft/s index values were statistically significant (p = 0.001). The simplified oral hygiene index of MIH patients were statistically higher, but no significant differences were found in modified gingival indices (p = 0.52). Although the salivary pH and flow rate of the patients in the study group were lower, the buffering capacity was higher than those in the control group, but no significant difference was observed (p = 0.64). The mean values of phosphorus, carbon and calcium content in the saliva samples of MIH patients were higher than those of patients without MIH, and this difference was low for phosphorus (p = 0.76) and carbon (p = 0.74), but significantly higher for calcium. To the best of our knowledge, this is the first study to evaluate the association between calcium, phosphate and carbon levels in saliva of children with MIH. The significantly high amount of calcium in the saliva of patients with MIH suggests that further investigations are needed.
Subject(s)
Dental Enamel Hypoplasia , Saliva , Humans , Saliva/chemistry , Child , Female , Male , Adolescent , Hydrogen-Ion Concentration , Minerals/analysis , Calcium/analysis , DMF Index , Severity of Illness Index , Phosphorus/analysis , Molar HypomineralizationABSTRACT
The aim of this study was to evaluate the 9-month clinical performance of different materials and treatment procedures in teeth with MIH in children, and to evaluate the effectiveness of Papacarie gel as a deproteinization agent. The study included 90 children (aged 8-15) who had 189 first permanent molars with MIH were restored randomly with 4 different materials/methods. Equia Forte HT (GC, Tokyo, Japan) was used in Group 1; In Group 2, G-eanial composite (GC, Tokyo, Japan) was used with a Fuji IX (GC, Tokyo, Japan) base; In Group 3 and Group 4, EverX Posterior (GC, Tokyo, Japan) base and G-eanial composite (GC, Tokyo, Japan) were used. In group 4, deproteinization was performed with Papacarie Duo gel (F&A, Sao Paulo, Brazil). The restorations were evaluated at 3-month intervals for 9 months using modified United States Public Health Service (USPHS) criteria. The overall recall rate was 94.1% for every 3-month clinical evaluation over 9 months. A total of 9 restorations were unsuccessful. Surface roughness of Group 1 was statistically different from all other groups in all control periods (p < 0.05). Marginal adaptation of Group 2 was found to be significantly different from Groups 3 and 4 at the both of 6th and 9th month controls. There was no significant difference between the groups in terms of retention, color match, marginal discoloration and secondary caries in all control months. Restoration of MIH with Equia Forte HT is almost as successful as composites. The use of dentin replacement materials instead of glass ionomer cements as a base in composite restorations shows better results. Papacarie deproteinization showed similar success with other composite groups. This study was the first clinical study in which Papacarie was used for deproteinization in teeth with MIH and will thus contribute to the literature.
Subject(s)
Dental Restoration, Permanent , Molar , Adolescent , Child , Female , Humans , Male , Composite Resins/therapeutic use , Dental Enamel Hypoplasia/therapy , Dental Restoration, Permanent/methods , Gels , Glass Ionomer Cements/therapeutic use , Papain/therapeutic use , Treatment OutcomeABSTRACT
In this work, a series of biguanide hydrochloride salts and their Cu(II) complexes were synthesized and screened for their acetyl/butyryl choline esterase inhibitory and antimicrobial properties. The structures of the synthesized compounds were characterised by common spectroscopic and analytical methods. Biguanide compounds showed considerably lower inhibitory activity compared to the reference drugs donepezil and galantamine. On the other hand, complexation of the biguanide compounds with Cu(II) resulted in dramatic increase in the inhibitory activity. The Cu(II) complexes showed AChE inhibitory activity with the IC50 values of 21.29±0.95-82.53±0.20â µM and those values are comparable to that of donepezil (IC50 : 18.54±1.03â µM). The synthesised compounds were also screened for their antimicrobial activity towards gram positive (+) and gram negative (-) bacteria. Compounds (12.50â mg/mL) showed important antibacterial properties with inhibition zones of 8-28â mm diameter against gram-positive and gram-negative microorganisms. Compounds A03 and A08 exhibited more antimicrobial properties towards E. coli than standard antibiotics amikacin and gentamicin.
Subject(s)
Anti-Infective Agents , Coordination Complexes , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Cholinesterases , Coordination Complexes/chemistry , Donepezil , Escherichia coli , Gram-Negative Bacteria , Ligands , Microbial Sensitivity Tests , Copper/chemistryABSTRACT
1,2,3-Triazole compounds (1a-3a) and their oxime derivatives (1b-3b) were synthesized. The structures of these synthesized compounds were characterized using common spectroscopic methods. Crystal structures of the compounds 3, 2b and 3b were determined by single crystal X-ray diffraction studies. The acetylcholinesteras (AChE) and butyrylcholinesterase (BChE) cholinesterase inhibitor (ChEI) and DNA/calf serum albumin (BSA) binding properties of the compounds were examined. DNA binding studies have shown that compounds interact with DNA through 1,2,3-triazole and oxime groups. When the binding constant Kb values were compared, it was revealed that compound 3b (Kb = 4.6 × 105 M-1) with oxime in its structure binds more strongly than the others. In addition, in vitro BSA binding studies showed that compounds 1b and 3b exhibited higher binding affinity. These results confirm that the quenching is due to the formation of a compound resulting from the static quenching mechanism, rather than being initiated by a dynamic mechanism. Likewise, when the enzyme activity of the compounds was examined, the compounds exhibited high inhibitory activity against AChE. The highest activity was observed for compounds 2b and 3b (8.6 ± 0.05 and 4.8 ± 0.052 µM). It was observed that the compounds were not selective with respect to BChE. Communicated by Ramaswamy H. Sarma.
ABSTRACT
Al3+ excess in the body can cause many diseases. The development of chemosensors for the detection of Al3+ is therefore highly desirable. A hydrazide Schiff base compound containing triphenylphosphonium units (ER) was prepared and used as fluorescence turn-on sensor for the sensing of Al3+. Detection of Al3+ among various metals has been achieved successfully through the formation of Al3+-ligand coordination complexes. To detect Al3+, the "turn on" property of the fluorogenic chemosensor was investigated. Fluorescence sensing studies were carried out in CH3OH-Water (v/v, 9/1, pH 7.0) at λem = 528 nm. The LOD for sensing of Al3+ was found to be 0.129 µM. Using Job's graph, the stoichiometric ratio of ER- Al3+ was determined to be 1:1. The binding constant was determined to be 1.7 × 107 M-1 between Al3 + and the chemosensor ER. Finally, the determination of Al3+ in real herbal teas was carried out by using the sensing function of the chemosensor ER.
ABSTRACT
Eight new hybrid constructs containing a series of sulfonamide and 1,2,3-triazole units were designed and synthesized. Anticancer, antioxidant and cholinesterase activities of these hybrid structures were investigated. In our design, the Cu(I)-catalyzed click reaction between N,4-dimethyl-N-(prop-2-yn-1-yl)benzenesulfonamide (6) and aryl azides 8a-h was used. Antioxidant activity values of 9f (IC50: 229.46 ± 0.001 µg/mL) and 9h (IC50: 254.32 ± 0.002 µg/mL) hybrid structures were higher than BHT (IC50: 286.04 ± 0.003 µg/mL) and lower than Ascorbic acid (IC50: 63.53 ± 0.001 µg/mL) and α-Tocopherol (IC50: 203.21 ± 0.002 µg/mL). We determined that the cytotoxic effects of hybrid constructs 9d (IC50: 3.81 ± 0.1084 µM) and 9g (IC50: 4.317 ± 0.0367 µM) against A549 and healthy cell line (HDF) are much better than standard cisplatin (IC50: 6.202 ± 0.0705 µM). It was determined that the AChE inhibitory activities of all synthesized compounds were much better than Galantamine used as a standard. In particular, 9c (IC50: 13.81 ± 0.0026 mM) had ten times better activity than the standard Galantamine (IC50: 136 ± 0.008 mM). The ADMET properties of the molecules have been thoroughly examined and met the criteria for drug-like substances. They also have a high oral absorption rate, as they can effectively cross the blood-brain barrier and are easily absorbed in the gastrointestinal tract. In vitro experiments were confirmed by in silico molecular docking studies.Communicated by Ramaswamy H. Sarma.
ABSTRACT
A novel donepezil-caffeic acid (DP-CA) hybrid molecule was designed, synthesis, and investigated by molecular modeling. Its biological activity and protective effect were investigated by the IR spectroscopy, 1H and 13C NMR spectroscopy, and mass spectrometry. DP-CA was highly active against acetylcholine esterase and inhibited it at the micromolar concentrations. Fluorescence and UV-Vis spectroscopy studies showed strong binding of DP-CA to DNA. Moreover, DP-CA exhibited protective effects against H2O2-induced toxicity in U-118 MG glioblastoma cells. Finally, molecular docking showed a high affinity of DP-CA in all concentrations, and the active 4EY7 site exhibited essential residues with polar and apolar contacts. Taken together, these findings indicate that DP-CA could be a prospective multifunctional agent for the treatment of neurodegenerative diseases.
Subject(s)
Acetylcholinesterase , Hydrogen Peroxide , Donepezil/pharmacology , Donepezil/chemistry , Molecular Docking Simulation , Prospective Studies , Acetylcholinesterase/chemistry , Acetylcholinesterase/metabolism , Cholinesterase Inhibitors/pharmacologyABSTRACT
A number of new biguanidine-sulfonamide derivatives (1-16) were synthesized and their structures were characterized by spectroscopic and analytical methods. Crystal structures of the compounds 1, 4, 8, 10 and 14 were determined by single crystal X-ray diffraction studies. X-ray crystallographic data showed the π-electron delocalization through the biguanide units. The AChE and BChE cholinesterase inhibitor, DPPH antioxidant and DNA/BSA binding properties of the synthesized compounds were evaluated. Results of cholinesterase inhibitory properties have shown that the compounds containing electron-withdrawing (-F, -Cl) groups have higher AChE/BChE inhibitory and antioxidant activities. Compound 3 showed higher BChE inhibitory activity than tacrine with IC50 value of 28.4 µM. The compounds interact with DNA via minor groove binding mode. The compounds with a naphthyl group in its structure strongly binds with DNA/BSA biomolecules.Communicated by Ramaswamy H. Sarma.
Subject(s)
Antioxidants , Cholinesterase Inhibitors , Cholinesterase Inhibitors/chemistry , Antioxidants/pharmacology , Antioxidants/chemistry , Acetylcholinesterase/chemistry , Tacrine/chemistry , Sulfanilamide , Molecular Docking Simulation , Structure-Activity RelationshipABSTRACT
A series of cyclic biguanidine derivatives (E01-E16) was designed, synthesized, characterized by FTIR, NMR, elemental analysis and evaluated as cholinesterase [acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE)] inhibitory effects for the treatment of Alzheimer's disease (AD). The cyclic biguanidine derivatives (E01-E16) were obtained as hydrochloride salt from one-pot reaction of 2-cyanoguanidine, p-substitute aniline derivatives and cyclohexanone/4-ethylcyclohexanone in the acidic medium. The crystal structures of compounds E13 and E16 were determined by XRD studies. The in vitro acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory assessment of the compounds revealed that compounds showed considerable inhibitory activity. The substitute groups on the phenyl rings have an impact on the inhibitory activity of the compounds. Compound E08, containing an ester group on the phenyl ring, showed the highest inhibitory activity against BuChE (IC50: 97.97 ± 0.13 µM) compared to other compounds. According to the docking result, compound E08 exhibited higher binding affinity (-10.17 kcal/mol) against BuChE than the standard drug tacrine (-7.02 kcal/mol). Compound E08 was orientated towards the active site of the enzyme BuChE.Communicated by Ramaswamy H. Sarma.
Subject(s)
Alzheimer Disease , Cholinesterase Inhibitors , Humans , Cholinesterase Inhibitors/chemistry , Butyrylcholinesterase/metabolism , Acetylcholinesterase/chemistry , Molecular Docking Simulation , Molecular Structure , Structure-Activity Relationship , Alzheimer Disease/drug therapyABSTRACT
Mono(M1-M5)- and di(DM1-DM5)-sulfonamide derivatives were synthesized by the reaction of 4-iodobenzenesulfonyl chloride compound and aniline derivatives in basic medium. The structures of sulfonamide derivatives were characterized by spectroscopic and X-ray diffraction methods. All compounds were screened for acetylcholinesterase (AChE) inhibitory studies and 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging activities. Among the compounds tested, compound M1 showed the best activity against both AChE (IC50 =42.09â µg/mL for AChE) and DPPH (IC50 =9.94â µg/mL for DPPH). By placing compounds at the active site of AChE, their binding energies and modes were determined. Docking studies were performed in order to investigate binding interaction between the synthesized compounds and AChE. The most active M1 compound showed its low CDOCKER energy (-65,834â kcal/mol).
Subject(s)
Acetylcholinesterase , Cholinesterase Inhibitors , Cholinesterase Inhibitors/chemistry , Acetylcholinesterase/metabolism , Antioxidants/chemistry , Chlorides , Molecular Docking Simulation , Sulfonamides/pharmacology , Sulfonamides/chemistry , Sulfanilamide , Structure-Activity RelationshipABSTRACT
BACKGROUND: In this study, the prevalence and distribution of dental caries and oral hygiene conditions in a group of patients with β-TM are evaluated and the results compared to age-and gender-matched healthy patients. In addition, oral candida colonization and the density of Streptococcus mutans (S.mutans) and Lactobacilli in the total saliva are assessed. MATERIAL AND METHODS: This study involved 59 β-TM patients between 6-16 years old (mean: 11.59 ± 3.22), who applied to the Department of Pedodontics, Faculty of Dentistry, Akdeniz University, with ongoing follow-up, treatment and regular blood transfusions. All enrolled patients were diagnosed with β-TM by the Department of Pediatric Hematology and Oncology, Faculty of Medicine, Akdeniz University. As a control group, age-and gender-matched healthy 50 patients were included to the study. RESULTS: Plaque (p = 0.001), DMFT (p = 0.009) and DMFS (p = 0.039) indices were significantly higher in the β-TM patients, whereas, the oral hygiene status was significantly lower (p = 0.004). Saliva buffering capacity average was insignificantly but slightly more in β-TM patients(p= 0.131).While S.mutans values were significantly higher in the β-TM patients (p = 0.002), no significant difference was found in the Lactobacillus (p = 0.131) and Candida values (p= 0.33). CONCLUSIONS: DMFT, DMFS, Plaque and oral hygiene indices and S.mutans values were found significantly differ-ent in β-TM patients than healthy, control group patients, in this study
No disponible
Subject(s)
Humans , Child , Adolescent , Dental Caries , Streptococcus mutans/isolation & purification , Lactobacillus/isolation & purification , Candida/isolation & purification , Saliva/microbiology , Oral Hygiene , DMF IndexABSTRACT
Objective: To identify epidemic and other transmissible Pseudomonas aeruginosa strains, genotypic analyses are required. The aim of this study was to assess the distribution of P. aeruginosa strains within the Turkish pediatric cystic fibrosis (CF) clinic population. Methods: Eighteen patients attending the pediatric CF clinic of Cerrahpasa Medical Faculty were investigated in the study. Throat swab and/or sputum samples were taken from each patient at 3-month intervals. The isolates of patients were analyzed by pulsed-field gel electrophoresis (PFGE). The intra- and interpatient genotypic heterogeneity of isolates was examined to determine the clonal isolates of P. aeruginosa within the cohort. Results: A total of 108 clinical isolates of P. aeruginosa were obtained from 18 patients between May 2013 and May 2014. The pulsotypes of the first patient's isolates could not be obtained by PFGE. From the remaining 17 patients and 101 isolates, 55 distinct pulsotypes were detected. The number of pulsotypes observed in more than one patient (minor clonal strains, cluster strains) was 8 (14.5%), and one of them colonized three patients. However, none of them was detected in more than three patients. These pulsotypes were composed of 20 isolates. In addition, with the PFGE analysis of 81 isolates, we detected 47 (85.6%) pulsotypes, which belonged to only one patient. Over different periods of this study, only 2 (11.8%) patients were colonized with the same pulsotype. Conclusion: Our study indicates that there was considerable genomic diversity among the P. aeruginosa isolates in our clinic. The presence of shared pulsotypes supports cross-transmission between patients.
ABSTRACT
BACKGROUND: Aspergillus fumigatus is the species section Fumigati most frequently isolated from the respiratory tract of cystic fibrosis (CF) patients. Recent studies suggest that mutations in the Cyp51 gene, particularly TR34/L98H, are responsible for azole resistance. OBJECTIVES AND METHODS: The focus of this study was on section Fumigati isolates isolated from the respiratory tract samples of CF patients. More specifically, the goal was to detect A. fumigatus isolates, test their antifungal susceptibility to itraconazole, voriconazole and posaconazole, and finally determine the presence of TR34/L98H and other mutations in the isolates Cyp51A gene. RESULTS AND CONCLUSIONS: A set of 31 isolates of Aspergillus section Fumigati were obtained from the sputum samples of 6 CF patients and subsequently identified to species level by microsatellite genotyping. All isolates were determined as A. fumigatus and involved 14 different genotypes. The minimal inhibitory concentrations to the three azoles were determined by the E-test method, and the Cyp51A gene was sequenced. One of the genotypes was found to be resistant to all azoles but no mutations were detected in the Cyp51A gene, especially the TR34/L98H mutation. Therefore, mutations in genes other than Cyp51A or other distinct mechanisms may be responsible for this reported multiazole resistance found in a Turkish CF patient.
Subject(s)
Antifungal Agents/pharmacology , Aspergillus fumigatus/drug effects , Azoles/pharmacology , Drug Resistance, Fungal , Mutation , Pulmonary Aspergillosis/microbiology , Sterol 14-Demethylase/genetics , Adolescent , Adult , Aspergillus fumigatus/genetics , Aspergillus fumigatus/isolation & purification , Child , Cystic Fibrosis/complications , Female , Genotype , Genotyping Techniques , Humans , Male , Microbial Sensitivity Tests , Microsatellite Repeats , Middle Aged , Mycological Typing Techniques , Sequence Analysis, DNA , Sputum/microbiology , Turkey , Young AdultABSTRACT
Tooth avulsion is a type of dental injury defined as the complete displacement of a tooth out of the alveolar socket, and the lack of prompt treatment measures can result in the loss of function, poor quality of life, and psychological and social problems. However, several factors may not permit the immediate replantation of an avulsed tooth; therefore, delayed replantation has emerged as an alternative to meet the esthetic, functional, and psychological demands of patients. Here it was described that the successful replantation of an avulsed maxillary central incisor in a 9-year-old boy who presented at the clinic with the tooth stored in unfavorable conditions as dry and then in olive oil-milk mixture almost 10 h after the event. The tooth has remained in its socket healthy for 16 years after treatment. The patient was satisfied with both esthetics and function.
Subject(s)
Incisor/injuries , Tooth Avulsion/surgery , Tooth Replantation/methods , Adult , Esthetics, Dental , Humans , Male , MaxillaABSTRACT
PURPOSE: The aim of this study was to compare the effects of 2 NiTi file systems [Twisted File Adaptive (TFA) and Reciproc (RP)] and 2 irrigation techniques [Conventional needle irrigation (CNI) and Laser activated irrigation (LAI)] on the amount of apically extruded debris in primary maxillary molars. STUDY DESIGN: Sixty extracted primary maxillary molars were randomly divided into 4 groups. The mesio-buccal roots of teeth in 4 groups were instrumented using TFA with LAI, RP with LAI, TFA with CNI and RP with CNI respectively. Debris extruded during instrumentation was collected into Eppendorf tubes and amounts were determined. The data were analyzed using two-way ANOVA test at 0.05 level of significance. RESULTS: The results indicated all instrumentation and irrigation systems caused a measurable apical extrusion of debris. However, no statistically significant difference in debris extrusion was observed between irrigation techniques or instrumentation systems (P > 0.05). The total amount of debris extruded apically by LAI was greater than that by CNI. CONCLUSIONS: All instrumentation and irrigation techniques caused debris extrusion. However, the optimal laser activation time and power settings should be investigated to ensure minimal extrusion of debris and irrigant due to cavitation and pressure during LAI in root canal treatment.
Subject(s)
Dental Alloys/chemistry , Foreign Bodies/etiology , Nickel/chemistry , Root Canal Irrigants/therapeutic use , Root Canal Preparation/instrumentation , Therapeutic Irrigation/methods , Titanium/chemistry , Tooth Apex/pathology , Tooth, Deciduous/pathology , Humans , In Vitro Techniques , Lasers, Solid-State , Molar/pathology , Needles , Random Allocation , Root Canal Irrigants/administration & dosage , Smear Layer/etiology , Therapeutic Irrigation/instrumentationABSTRACT
BACKGROUND: The aim of the present study was to determine Shear bond strength (SBS) of different flowable compomers on the enamel surface of primary teeth. The null hypothesis to be tested was that none of the flowable compomer would differ significantly from the other two with respect to SBS. As a result, the tested materials that have the easiest application on child patient is preferred. MATERIAL AND METHODS: Sixty newly extracted non carious primary molars were selected. The buccal surface was cleaned and polished to obtain a flat enamel surface. The specimens were randomly divided into three groups of 20 teeth each, based on the flowable compomers applied, as follows: group I: Dyract Flow® (Dentsply, Konstanz, Germany); group II: Twinky Star Flow® (Voco, Cuxhaven, Germany); and group III: R&D Series Nova Compomer Flow® (Imicryl, Konya, Turkey). RESULTS: SBS in group II (6.78± 0.45 MPa) were significantly lower than groups I and III (8.30 ± 0.29 and 8.43 ± 0.66 MPa, respectively) (P<.001). No significant difference was found between groups I and III (P<.05). CONCLUSIONS: Significant differences existed between the SBS of the groups. Therefore, the null hypothesis was rejected. Flowable compomers can provide adequate SBS with self-etching system at restoration of primary teeth. Thus, successful restorations in pediatric patients can be done in a practical way. KEY WORDS: Flowable compomer, primary teeth, shear bond strength.
ABSTRACT
INTRODUCTION: The aim of the study was to evaluate the in vitro microleakage of new flowable compomers in the class V cavities of primary teeth. METHODS: Thirty freshly extracted, non-carious, primary molars without visible defects were used in this study. Class V cavities (n = 60), with the occlusal and cervical margins located in the enamel, were prepared on the buccal and lingual surfaces. The samples were randomly divided into 3 groups of 20 each. Group 1: restored with Twinky Star Flow (Voco, Cuxhaven, Germany), Group 2: restored with Dyract Flow (Dentsply, Konstanz, Germany) and Group 3: restored with R&D Series Nova Compomer Flow (Imicryl, Konya, Turkey) according to the manufacturer's instructions. After a thermocycling regimen of 1000 cycles between 5°C and 55°C, the samples were isolated, immersed in 0.5% basic fuchsine solution for 24 h at 37°C and sectioned longitudinally in a buccolingual direction. The sections were evaluated for values of microleakage with a stereomicroscope. RESULTS: All materials showed microleakage but no statistically significant difference was observed among the groups (p>0.05). The highest microleakage score was observed in group II (1.65 ± 0.49) and group I (1.75 ± 0.44) at occlusal and gingival margins, respectively. CONCLUSIONS: Flowable compomers showed insignificantly least amount of microleakage in class V cavities prepared on primary molars.
Subject(s)
Compomers , Dental Cavity Preparation , Dental Leakage/diagnosis , Dental Restoration, Permanent , Molar/surgery , Tooth, Deciduous/surgery , Composite Resins , Humans , Rosaniline DyesABSTRACT
AIM: Triazole resistance in Aspergillus fumigatus is associated with mutations in cyp51 genes, therefore, a single-tube multiplex PCR was proposed for rapid detection of such mutations. METHODS: Relevant markers (n = 21) located in cyp51A and cyp51B were amplified in a multiplex reaction and subsequently analyzed by mini-sequencing. RESULTS: A set of nonresistant A. fumigatus were tested. The markers F46, G89, M172, D255, L358, E427 and C454 located in cyp51A, as well as P394 and S35 from cyp51B, were found to be modified. CONCLUSIONS: A. fumigatus triazole resistance in Portugal is rare, nevertheless, some isolates showed alterations in the cyp51 genes. Multicenter studies with more isolates should better evaluate and validate the potential use of this method in clinical laboratories. The new methodology allows the addition of extra markers if described as relevant for A. fumigatus susceptibility to triazoles.