Is scanning under rubber dam isolation a feasible approach for the execution of indirect restorations? / O escaneamento sob isolamento absoluto é uma técnica viável para execução de restaurações indiretas?

Objective: The aim of the study was to report the aplicability of intraoral scanning while rubber dam isolation is in place. Material and Methods: Female patient, 50 years old, required restorative procedures on teeth 35 and 37. An intraoral scan was initially performed on both arches. Isolation was carried out from 33 to 37, tooth preparation and immediate dentin sealing were carried out. A new scan with the rubber dam in place was performed and a CAD/CAM lithium disilicate hybrid block was digitally designed, milled, crystallized and cemented under the tooth surface with the rubber dam still in position. After completing this stage, the rubber dam was removed, the occlusion was verified, presenting excellent aesthetic and functional results. Results: The absolute isolation process used in the present study works as an excellent device for gingival retraction. Conclusion: The absolute isolation can be recommended in clinical activities of intraoral scanning favoring the quality of the final result of treatments (AU)

Objetivo: O objetivo do estudo foi relatar a aplicabilidade do escaneamento intraoral sob isolamento absoluto. Material e Métodos: Paciente do sexo feminino, 50 anos, necessitou de procedimentos restauradores nos dentes 35 e 37. Uma varredura intraoral foi inicialmente realizada em ambos os arcos. O isolamento absoluto foi feito de 33 a 37, permitindo a realização do preparo dentário e selamento imediato da dentina. Um novo escaneamento com o dique de borracha colocado foi realizado e um bloco híbrido de dissilicato de lítio CAD/CAM foi projetado digitalmente, fresado, cristalizado e cimentado sob a superfície dentária ainda com o dique de borracha em posição. Após a finalização dessa etapa, o dique de borracha foi removido, a oclusão foi verificada apresentando ótimos resultados estéticos e funcionais. Resultados: O isolamento absoluto utilizado no presente estudo funciona como um excelente dispositivo para retração gengival. Conclusão: O isolamento absoluto pode ser recomendado em atividades clínicas de escaneamento intraoral favorecendo a qualidade do resultado final dos tratamentos (AU)

Clinicopathological Features of Metastasis to the Oral and Maxillofacial Region-Multicenter Study.

BACKGROUND: This study aimed to investigate the clinicopathological features of metastases in the oral and maxillofacial regions. METHODS: In this retrospective study, biopsy records were obtained from referral centers for oral and maxillofacial diagnosis in Brazil, Guatemala, Mexico, and South Africa. RESULTS: A total of 120 cases were evaluated. Of these, 53.78% affected female patients, with a mean age of 57.64 years. Intraosseous lesions were more frequent, particularly in the posterior region of the mandible (49.58%). Clinically, most cases presented with symptomatic swelling, with an average evolution time of 25 months. The clinical diagnostic hypothesis in most instances was that of a malignant lesion. Breast cancer was the most common primary tumor location in females, while lung origin was most common in males. In most cases, the primary cancer was an adenocarcinoma (44.73%). The follow-up period was available for 29 cases, and out of these, 20 had died due to the disease. CONCLUSION: Although this is a rare condition, clinicians should be aware that any oral lesions have the possibility of being metastatic, particularly in individuals with a previous history of cancer. The findings from this study could assist clinicians in prompt diagnosing these lesions and subsequent conducting oncologic assessments and treatment.

Extracellular Vesicles Contribute to the Difference in Lipid Composition between Ovarian Follicles of Different Size Revealed by Mass Spectrometry Imaging.

Follicular fluid (FF) ensures a safe environment for oocyte growth and maturation inside the ovarian follicle in mammals. In each cycle, the large dominant follicle (LF) contains the oocyte designated to be ovulated, whereas the small subordinate follicles (SFs) of the same wave will die through atresia. In cows, the oocytes from the SF, being 2 mm in size, are suitable for in vitro reproduction biotechnologies, and their competence in developing an embryo depends on the size of the follicles. FF contains proteins, metabolites, fatty acids, and a multitude of extracellular vesicles (ffEVs) of different origins, which may influence oocyte competence through bidirectional exchanges of specific molecular cargo between follicular cells and enclosed oocytes. FF composition evolves along with follicle growth, and the abundance of different lipids varies between the LF and SF. Here, significant differences in FF lipid content between the LFs and SFs within the same ovary were demonstrated by MALD-TOF mass spectrometry imaging on bovine ovarian sections. We then aimed to enlighten the lipid composition of FF, and MALDI-TOF lipid profiling was performed on cellular, vesicular, and liquid fractions of FF. Differential analyses on the abundance of detected lipid features revealed specific enrichment of phospholipids in different ffEV types, such as microvesicles (MVs) and exosomes (Exo), compared to depleted FF. MALDI-TOF lipid profiling on MVs and Exo from the LF and SF samples (n = 24) revealed that more than 40% of detected features were differentially abundant between the groups of MVs and Exo from the different follicles (p < 0.01, fold change > 2). Glycerophospholipid and sphingolipid features were more abundant in ffEVs from the SFs, whereas different lysophospholipids, including phosphatidylinositols, were more abundant in the LFs. As determined by functional analysis, the specific lipid composition of ffEVs suggested the involvement of vesicular lipids in cell signaling pathways and largely contributed to the differentiation of the dominant and subordinate follicles.

Acinic cell carcinoma of the oral and maxillofacial region: an international multicenter study.

The aim of this study was to describe the prevalence, clinicopathological, and prognostic features of acinic cell carcinoma (AciCC) of the oral and maxillofacial region. AciCC cases were retrospectively retrieved from 11 pathology centers of three different countries. Medical records were examined to extract demographic, clinical, pathologic, and follow-up information. A total of 75 cases were included. Females (65.33%) with a mean age of 45.51 years were mostly affected. The lesions usually presented as an asymptomatic (64.28%) nodule (95.66%) in the parotid gland (70.68%). The association of two histopathological patterns was the most common finding (48.93%) and the tumors presented mainly conventional histopathological grades (86.11%). Surgical treatment was performed in the majority of the cases (59.19%). Local recurrence was observed in 20% of the informed cases, regional metastasis in 30.43%, and distant metastasis in 12.50%. The statistical analysis showed that the cases with a solid histopathological pattern (p=0.01), high-grade transformation (p=0.008), recurrence (p=0.007), and regional metastasis (p=0.03) were associated with poor survival. In conclusion, high histopathological transformation, presence of nodal metastasis, and recurrence were prognostic factors for AciCC of the oral and maxillofacial region.

Effects of Hippotherapy on Postural Control in Children With Cerebral Palsy: A Systematic Review.

PURPOSE: To summarize and update the current knowledge on the effectiveness of hippotherapy on postural control in children with cerebral palsy. METHODS: Using a systematic review methodology, the electronic databases PubMed, Virtual Health Library, PEDro, Scielo, Embase, and Web of Science were searched for eligible articles from 2011 up to September 2021. Quality assessment of eligible studies was performed using the PEDro scale. RESULTS: There were 239 identified studies. Eight clinical trials were selected. The total sample consisted of 264 individuals, with 134 allocated to the experimental group (hippotherapy) and 130 individuals to the control group (conventional therapy). Most studies had moderate to high methodological quality. CONCLUSIONS: Hippotherapy can be an effective intervention to improve several aspects related to postural control, such as static balance (especially in the sitting posture), dynamic balance, and alignment (body posture) in children aged 3 to 16 years, particularly with spastic hemiplegia or diplegia. WHAT THIS ADDS TO THE EVIDENCE: This review summarizes studies that explore potential effects of hippotherapy on postural control in children with cerebral palsy.

Acinic cell carcinoma of the oral and maxillofacial region: an international multicenter study

Abstract The aim of this study was to describe the prevalence, clinicopathological, and prognostic features of acinic cell carcinoma (AciCC) of the oral and maxillofacial region. AciCC cases were retrospectively retrieved from 11 pathology centers of three different countries. Medical records were examined to extract demographic, clinical, pathologic, and follow-up information. A total of 75 cases were included. Females (65.33%) with a mean age of 45.51 years were mostly affected. The lesions usually presented as an asymptomatic (64.28%) nodule (95.66%) in the parotid gland (70.68%). The association of two histopathological patterns was the most common finding (48.93%) and the tumors presented mainly conventional histopathological grades (86.11%). Surgical treatment was performed in the majority of the cases (59.19%). Local recurrence was observed in 20% of the informed cases, regional metastasis in 30.43%, and distant metastasis in 12.50%. The statistical analysis showed that the cases with a solid histopathological pattern (p=0.01), high-grade transformation (p=0.008), recurrence (p=0.007), and regional metastasis (p=0.03) were associated with poor survival. In conclusion, high histopathological transformation, presence of nodal metastasis, and recurrence were prognostic factors for AciCC of the oral and maxillofacial region.

Effects of different amendments on the quality of burnt eucalypt forest soils - A strategy for ecosystem rehabilitation.

The magnitude of forest fires' impacts on the environment is directly related to the changes induced on soil physical, chemical and biological properties. Using available organic resources to rehabilitate burnt forest soils can help reduce post-fire soil fertility loss, accelerating ecosystem recovery. In the present study, the potential of four soil amendments: a mycotechnosol, a eucalypt residue mulch, dredged sediments from a freshwater lagoon and an organic-mineral biofertilizer, to improve the quality of burnt forest soils in terms of organic matter, carbon, nitrogen and phosphorus contents, was evaluated. Two experiments were set-up, one in a recently burnt eucalypt plantation and another in the laboratory using soils from the same area, to assess the effects of the amendments on soil quality, with both experiments lasting for 7 months. The effects of the amendments on nutrient leaching along the soil profile were also evaluated in the laboratory, to investigate possible negative impacts on groundwater and surface water quality. All amendments increased the organic matter and nutrient contents of burnt soils, confirming their potential for ecosystem rehabilitation. The biofertilizer, however, was found to promote nutrient losses by leaching, largely owing to its high solubility, increasing the risk of contamination of ground and surface waters. Using available organic resources to rehabilitate burnt forests as was done in the present work complies with the idea of a circular economy, being key for the sustainability of forest ecosystems.

Does smoking habit affect dendritic cell expression in oral squamous cell carcinoma?

The aim of this study was to determine the presence of immature CD1a+ and mature CD83+ dendritic cells in oral squamous cell carcinoma, to compare immunoreactivity between smokers and nonsmokers, and to correlate the results with histopathological grading. In this observational study, twenty-eight paraffin-embedded biopsies of oral squamous cell carcinoma were retrospectively retrieved and submitted to immunohistochemistry for immature CD1a+ and mature CD83+. Descriptive and statistical analyses were performed. The sample consisted of 18 man (64.3%) and 10 women (35.7%), with a mean age of 64.6 years in the nonsmoker group and 53.2 years in the smoker group. The tongue (11 cases, 39.2%) was the most commonly affected anatomical site, followed by gingiva (6 cases, 21.4%). Histopathological grading revealed 7 low-grade and 7 high-grade malignancy cases in each group, and no correlation with the number of positive DCs. The number of immature CD1a+ was not significantly different between smoker and nonsmoker groups, while a lower number of mature CD83+ was detected in the smoker group (p = 0.001). Smoking changes the oral immune system and decreases the ability to activate and mature dendritic cells, which may influence the development and progression of oral squamous cell carcinoma.

Glandular odontogenic cysts: A collaborative investigation of 22 cases and proteins related to invasiveness.

BACKGROUND: A glandular odontogenic cyst (GOC) has an intriguing, aggressive behaviour whose mechanisms have not yet been clarified. OBJECTIVE: To conduct a collaborative cross-sectional study on the clinical, demographic, microscopic and immunohistochemical characteristics of GOCs, emphasizing the histopathological characteristics and expression of proteins related to invasiveness. METHODS: Twenty-two cases of GOC from three oral and maxillofacial pathology services in Brazil were selected from 1988 to 2018. Clinical and demographic data were collected. Histopathological features were evaluated in detail. Sixteen cases of GOC were also submitted to immunohistochemistry to detect MT1-MMP, TKS4, TKS5 and cortactin, the key regulators of invadopodia formation. RESULTS: Glandular odontogenic cysts were primarily seen in men over 40 years of age, in the posterior mandible and the anterior maxilla as a unilocular, radiolucent lesion. All cases presented hobnail cells, clear cells and variable thickness of the lining epithelium, 3 of the 10 key histopathological parameters to be evaluated in GOCs. Immunohistochemistry revealed a greater expression of the studied proteins in the GOCs than in the controls (p < 0.0001). CONCLUSION: Overexpression of proteins that regulate cell invasiveness was identified, and the present study's findings suggest that invadopodia activity is a possible mechanism used by GOCs to promote local invasion, which could partly explain its intriguing biological behaviour.

Impact of the COVID-19 pandemic on public University laboratories of oral and maxillofacial pathology: A Brazilian multicenter study.

OBJECTIVE: To evaluate the impact of the COVID-19 pandemic on the number of diagnoses of oral and maxillofacial lesions in public laboratories after one year of COVID-19 outbreak in Brazil. MATERIAL AND METHODS: This is a cross-sectional study. Biopsies submitted to histopathologic examination from March 2019 to February 2020 (pre-pandemic period) and from April 2020 to March 2021 (pandemic period) in nine Brazilian public oral pathology laboratories were retrieved and the number of diagnoses, types of lesion, and percentage changes during both periods were analyzed. RESULTS: There were 7389 diagnoses in the pre-pandemic period and 2728 in the pandemic era, indicating a reduction of 63.08%. The reduction was 64.23% for benign lesions and 49.48% for malignant lesions, with a 50.64% reduction in squamous cell carcinoma. The largest decreases were observed in April 2020 and January 2021. CONCLUSION: An important reduction in the diagnoses of benign and malignant lesions was noted in the Brazilian public oral pathology laboratories during the first year of the COVID-19 pandemic.

Sexo, estadiamento puberal, hábitos alimentares e de vida e desfechos clínico-nutricionais na saúde adolescente / Sex, pubertal stage, eating and lifestyle habits and clinical-nutritional outcomes in adolescent health

Objetivo: Avaliar a associação entre sexo, variáveis sociodemográficas, maturacionais, alimentares e de estilo de vida e desfechos clínico-nutricionais. Métodos: Estudo transversal com 232 adolescentes entre 12 e 19 anos do projeto Camelia, realizado em Niterói, Rio de Janeiro, em 2006 e 2007, pela Universidade Federal Fluminense. Os participantes foram submetidos a questionário, consulta médica, mensuração da pressão arterial, coleta de sangue e urina, avaliação antropométrica, maturacional e nutricional. Modelos de regressão linear e logística foram utilizados para avaliar a associação entre o sexo, variáveis sociodemográficas, hábitos alimentares e estilo de vida com os desfechos: Índice de Massa Corporal (IMC), % de Gordura Corporal (%GC), lipoproteína de baixa densidade (LDL); glicemia, colesterol e pressão arterial, segundo o estadiamento puberal. Resultados: Hábitos saudáveis como o consumo semanal de feijão igual ou superior a cinco vezes impactaram positivamente no IMC, %GC, LDL, e no colesterol e glicose (até 2 vezes ao dia) (ambos no estadiamento puberal inicial); e o consumo de frutas (duas a três vezes por dia), e vegetais (três ou mais vezes por dia) no colesterol (no estadiamento puberal avançado). Hábitos deletérios como o consumo de açúcar ou refrigerantes ou sucos industrializados maior ou igual a 3 colheres de sobremesa ou 2 vezes ao dia ou 5 vezes na semana foram associados a piora do LDL, da glicemia (no estadiamento puberal inicial), do colesterol (em ambos estadiamentos) e da pressão arterial (no estadiamento puberal avançado), assim como despender 2 ou mais horas por dia em atividades sedentárias da glicemia (no estadiamento puberal inicial). A escolaridade materna fundamental associou se a glicemias superiores (no estadiamento puberal inicial) e a renda per capita intermediária à redução do LDL e do colesterol (no estadiamento puberal avançado). A omissão do almoço e do desjejum ocasionaram aumentos no IMC e %GC, e no LDL, respectivamente. E almoçar fora de casa (no estadiamento puberal inicial) e o consumo de frituras maior ou igual a 5 vezes por semana (no estadiamento puberal avançado) a níveis pressóricos mais altos. O sexo se associou à glicemia, colesterol e pressão arterial, somente no estadiamento puberal avançado, sendo desfavorável para o sexo masculino, nos desfechos glicemia (exceto filhos de mães com escolaridade igual ou superior ao 1º ano do ensino médio e, principalmente, nos mais sedentários e nos consumidores de suco industrializado em frequências iguais ou superiores a cinco vezes na semana) e pressão arterial; e para o sexo feminino no colesterol (exceto nas que consumiam frutas duas a três vezes por dia). Conclusão: O consumo de feijão despontou como hábito alimentar relevante para a saúde dos adolescentes, podendo este ser considerado uma "proxy" de alimentação saudável, ao passo que hábitos característicos de dietas e estilo de vida não saudáveis impactaram negativamente, mostraram-se sexualmente assimétricos e se intensificaram com o tempo, indicando que a prevenção deve ocorrer ainda nas idades iniciais da adolescência e deve considerar as diferenças inerentes ao sexo.

Investigating the association of individuals' sex, sociodemographic, maturational, dietary and lifestyle variables with clinical and nutritional outcomes. Methods: Cross-sectional study conducted with 232 adolescents in the age group 12-19 years, who participated in the Camelia project that was carried out by Fluminense Federal University, in Niterói County, Rio de Janeiro State, in 2006 and 2007. Participants were subjected to questionnaire application, medical consultation, blood pressure measurement, blood and urine sample collection, as well as to anthropometric, maturational and nutritional assessment. Linear and logistic regression models were used to assess the association of individuals' sex, sociodemographic variables, eating habits and lifestyle with the following outcomes: Body Mass Index (BMI), Body Fat % (BF%), low-density lipoprotein (LDL), blood glucose, cholesterol and blood pressure, based on pubertal stage. Results: Healthy habits, such as intake of beans equal to, or higher than, five times a week, had positive impact on BMI, BF%, LDL, as well as on cholesterol and glucose levels (up to 2 times a day) (both at initial pubertal stage); whereas fruits (two to three times a day) and vegetables' (three, or more, times a day) intake had positive impact on cholesterol levels (at advanced pubertal stage). Deleterious habits such as the intake of sugar, soft drinks or industrialized juices, at amounts higher than, or equal to, 3 dessert spoons or 2 times a day or 5 times a week were associated with worsened LDL, blood glucose (at initial pubertal stage), cholesterol (at both pubertal stages) and blood pressure (at advanced pubertal stage), as well as spending 2, or more, hours a day in sedentary activities was associated with worsened blood glucose (at initial pubertal stage). Primary maternal schooling was associated with higher blood glucose levels (at initial pubertal stage) and intermediate income per capita was associated with reduced LDL and cholesterol levels (at advanced pubertal stage). Skipping lunch and breakfast led to increased BMI and BF%, as well as to increased LDL, respectively. Having lunch out (at initial pubertal stage) and fried food intake higher than, or equal to, 5 times a week (at advanced pubertal stage) were associated with increased blood pressure. Sex was only associated with blood glucose, cholesterol and blood pressure at advanced pubertal stage; male participants recorded unfavorable blood glucose (except for children from mothers with schooling equal to, or higher than, the 1st year of high school; and mainly in the most sedentary individuals and in those who consumed industrialized juice at frequency equal to, or higher than, five times a week) and blood pressure outcomes; whereas female participants recorded unfavorable cholesterol outcome (except for those who consumed fruit two to three times a day). Conclusion: The intake of beans has emerged as relevant food habit for adolescents' health; thus, it can be considered a "proxy" of healthy eating, whereas habits typical of unhealthy diets and lifestyles had negative impact on participants' health. These habits were sexually asymmetrical and got worse overtime, which indicated the need of taking preventive measures in early adolescence to avoid the development of such habits by taking into account differences inherent to sex.

Occurrence of potential wild hosts of Echinococcus vogeli in the forests of southwestern Brazilian Amazonia / Ocorrência de potenciais hospedeiros silvestres de Echinococcus vogeli nas florestas do sudoeste da Amazônia brasileira

Abstract The helminth Echinococcus vogeliRausch & Bernstein, 1972 is a causative agent of Neotropical Echinococcosis, a chronic zoonotic disease which is endemic to the Neotropical region. This parasite is transmitted from bush dogs (Speothos venaticus) to their prey, which include lowland pacas (Cuniculus paca) and agoutis (Dasyprocta spp.). In Brazil, most human cases of Neotropical Echinococcosis have been recorded in the Amazonian states of Acre and Pará, although few data are available on the occurrence of the potential definitive or intermediate hosts of E. vogeli in the Amazon region. In the present study, we surveyed the forests surrounding 46 human communities located within and around of outside six sustainable-use protected areas in the southwestern Amazon basin of Brazil. The forests were surveyed using camera traps to determine the local presence of potential wild hosts of E. vogeli, and the exploitation of these hosts for game meat was evaluated through interviews with 136 subsistence hunters resident in the local communities. We recorded pacas, agoutis, and bush dogs, as well as domestic dogs (Canis familiaris), all potential reservoirs of Neotropical Echinococcosis, using the same habitats. We also confirmed the frequent consumption of paca and agouti meat by subsistence hunters and their families in the study communities. Our data contribute to the understanding of the occurrence of E. vogeli in Brazilian ecosystems.

Resumo O helminto Echinococcus vogeliRausch & Bernstein, 1972 é o agente causador da Equinococose Neotropical, uma doença zoonótica crônica e endêmica da região Neotropical. Este parasito é transmitido entre o cachorro-vinagre (Speothos venaticus) e suas presas, como pacas (Cuniculus paca) e cutias (Dasyprocta spp.). No Brasil, a maioria dos casos humanos de Equinococose Neotropical é registrada nos estados do Acre e Pará, embora existam poucos dados disponíveis sobre a ocorrência de potenciais hospedeiros definitivos e intermediários de E. vogeli na Amazônia. No presente estudo, foram investigadas áreas de floresta ao redor de 46 comunidades humanas localizadas no interior e entorno de seis unidades de conservação de uso sustentável no sudoeste da bacia amazônica brasileira e, por meio de armadilhas fotográficas, foram avaliadas as presenças de potenciais hospedeiros silvestres de E. vogeli. Adicionalmente, foram avaliados o padrão de consumo da carne dos hospedeiros silvestres por meio de entrevistas com 136 moradores dessas comunidades. Foram registradas pacas, cutias e cachorros-vinagre, bem como cães domésticos (Canis familiaris) utilizando os mesmos habitats, todos potenciais reservatórios da Equinococose Neotropical. Além disto, confirmamos a alto consumo de paca e cutia nas comunidades. Os dados do presente trabalho contribuem para pesquisas em andamento sobre a presença dos potenciais reservatórios de E. vogeli em ambientes brasileiros.

Does smoking habit affect dendritic cell expression in oral squamous cell carcinoma?

Abstract: The aim of this study was to determine the presence of immature CD1a+ and mature CD83+ dendritic cells in oral squamous cell carcinoma, to compare immunoreactivity between smokers and nonsmokers, and to correlate the results with histopathological grading. In this observational study, twenty-eight paraffin-embedded biopsies of oral squamous cell carcinoma were retrospectively retrieved and submitted to immunohistochemistry for immature CD1a+ and mature CD83+. Descriptive and statistical analyses were performed. The sample consisted of 18 man (64.3%) and 10 women (35.7%), with a mean age of 64.6 years in the nonsmoker group and 53.2 years in the smoker group. The tongue (11 cases, 39.2%) was the most commonly affected anatomical site, followed by gingiva (6 cases, 21.4%). Histopathological grading revealed 7 low-grade and 7 high-grade malignancy cases in each group, and no correlation with the number of positive DCs. The number of immature CD1a+ was not significantly different between smoker and nonsmoker groups, while a lower number of mature CD83+ was detected in the smoker group (p = 0.001). Smoking changes the oral immune system and decreases the ability to activate and mature dendritic cells, which may influence the development and progression of oral squamous cell carcinoma.

Produção científica sobre fluorose em regiões endêmicas: estudo bibliométrico de Anais das Reuniões da Sociedade Brasileira de Pesquisa Odontológica / Scientific production on fluorosis in endemic regions: bibliometric study of Meeting Annals of the Brazilian Society of Dental Research

Objetivo: Identificar os resumos publicados nas Reuniões Anuais da Sociedade Brasileira de Pesquisa Odontológica sobre fluorose em regiões endêmicas e verificar a contribuição da Ciência Brasileira para o assunto. Métodos: Este estudo descritivo foi realizado com dados secundários do período entre 2005 e 2020; os estudos elegíveis foram identificados a partir dos descritores presentes no título e/ou resumo: flúor, fluorose, fluorose dental, fluorose dentária, fluoretação, intoxicação por flúor. Critérios de inclusão: estudos em regiões declaradas como de fluorose endêmica ou que citassem o teor de flúor acima de 0,8 mg F/L. Foram excluídos estudos que não declarassem estudar fluorose em regiões endêmicas ou não citassem o teor de flúor na água. Os dados foram coletados por dois examinadores de modo independente e a distribuição de frequências dos dados foi realizada (Microsoft Excel para Mac v.16.16.12). Resultados: Observou-se que de 41.953 resumos publicados durante esses 15 anos, 1190 (2,83%) foram elegíveis; 426 (1,01%) abordaram especificamente o tema fluorose e24 (0,06%) a fluorose em regiões endêmicas. Os assuntos abordados envolveram tratamento (n = 4; 14,81%); desenvolvimento de programas de promoção de saúde bucal (n = 2; 7,69%);avaliação da potabilidade da água (n = 2; 7,41%); implementação de sistemas de desfluoretação (n = 2; 7,41%); prevalência e severidade (n = 2; 7,41%); distribuição da fluorose na dentição (n = 1, 3,70%); impacto na qualidade de vida (n = 2; 7,41%); percepçãoestética(n = 5; 18,52%); uso e ingestão de flúor (n = 3; 11,11%); fluorose óssea (n = 2; 7,41%); análise da possibilidade de alteração no padrão cognitivo (n = 1; 3,70%), nas funções motoras e no equilíbrio (n=1; 3,70%).Conclusão: Diferentes aspectos relacionados à fluorose em região endêmica foram abordados. A Ciência Brasileira tem se dedicado ao tema ao longo dos anos. Entretanto, há um número restrito de estudos, em especial sobre a relação dose-reposta.

Aim: Identify the abstracts published at the Brazilian Society for Dental Research's Annual Meetings regarding Fluorosis in endemic regions, as well as verify the contribution of Brazilian science to the subject. Methods:This descriptive study was conducted using secondary data from the period between 2005 and 2020; the eligible studies were identified from the descriptors present in the title and/or abstract, as follows: fluoride, fluorosis, dental fluorosis, dental fluorosis, fluoridation, fluoride poisoning. Inclusion criteria: studies in regions with endemic fluorosis were included. Studies that did not address fluorosis in endemic regions were excluded. Data were collected by two independent examiners and the distribution frequency of the data was performed (Microsoft Excel for Mac v.16.16.12). Results: It was observed that of the 41,953 abstracts published during these 15 years, 1,190 (2.83%) were eligible; 426 (1.01%) specifically addressed the theme of fluorosis, and of these, 24 (0.06%) reported on fluorosis in endemic regions. The subjects addressed involved treatment (n = 4; 14.41%), the development of oral health promotion programs (n = 2; 7.41%), the evaluation of water potability (n = 2; 7.41%), the implementation of defluoridation systems (n = 2; 7.41%), prevalence and severity (n = 2; 7.41%), the distribution of fluorosis in dentition (n = 1, 3.70%), impacts on the quality of life (n = 2; 7.41%), esthetic perceptions (n = 4; 18.52%), fluoride use and ingestion (n = 3; 11.11%), bone fluorosis (n = 2; 7.41%), the analysis of alterations in the cognitive pattern (n = 1; 3.70%) and change in motor functions and balance (n=1; 3,70%).Conclusion: Different aspects related to fluorosis in an endemic region were addressed. Brazilian Science has been devoted to the theme over the years; however, there have been a limited number of studies published in the literature concerning the dose-response ratio.

Protein Palmitoylation in Bovine Ovarian Follicle.

Protein palmitoylation is a reversible post-translational modification by fatty acids (FA), mainly a palmitate (C16:0). Palmitoylation allows protein shuttling between the plasma membrane and cytosol to regulate protein stability, sorting and signaling activity and its deficiency leads to diseases. We aimed to characterize the palmitoyl-proteome of ovarian follicular cells and molecular machinery regulating protein palmitoylation within the follicle. For the first time, 84 palmitoylated proteins were identified from bovine granulosa cells (GC), cumulus cells (CC) and oocytes by acyl-biotin exchange proteomics. Of these, 32 were transmembrane proteins and 27 proteins were detected in bovine follicular fluid extracellular vesicles (ffEVs). Expression of palmitoylation and depalmitoylation enzymes as palmitoyltransferases (ZDHHCs), acylthioesterases (LYPLA1 and LYPLA2) and palmitoylthioesterases (PPT1 and PPT2) were analysed using transcriptome and proteome data in oocytes, CC and GC. By immunofluorescence, ZDHHC16, PPT1, PPT2 and LYPLA2 proteins were localized in GC, CC and oocyte. In oocyte and CC, abundance of palmitoylation-related enzymes significantly varied during oocyte maturation. These variations and the involvement of identified palmitoyl-proteins in oxidation-reduction processes, energy metabolism, protein localization, vesicle-mediated transport, response to stress, G-protein mediated and other signaling pathways suggests that protein palmitoylation may play important roles in oocyte maturation and ffEV-mediated communications within the follicle.

Use of MALDI-TOF mass spectrometry to explore the peptidome and proteome of in-vitro produced bovine embryos pre-exposed to oviduct fluid.

In order to identify oviduct fluid (OF) peptides and proteins possibly uptaken by developing embryos, in-vitro produced bovine embryos exposed or not to OF were individually analyzed by MALDI-TOF mass spectrometry. Overall, 11 masses were overabundant in OF-treated embryos compared to controls, among which one at 8.9 kDa annotated as immediate early response 3-interacting protein 1 or a peptide of transitional endoplasmic reticulum ATPase met the criteria of an OF embryo-interacting protein or peptide.

[Adequacy of the frequency and types of meals consumed by community-dwelling elderly folk in the south of Brazil]. / Adequação da frequência e tipos de refeições realizadas por idosos comunitários do sul do Brasil.

The scope of this study was to describe the frequency and types of meals consumed by the elderly in Pelotas (Brazil) and factors associated with the adequacy of meal frequency. A cross-sectional study was carried out with ≥60 years of age individuals. They were asked about daily meals during the week prior to the interview. The adequacy of meals was defined as recommended by the Ministry of Health (at least three main meals and two small snacks per day). The independent variables were sociodemographic, health and food routine characteristics. Prevalence ratios and 95% confidence intervals were calculated using Poisson regression. Of the 1,438 elderly individuals interviewed, about 40% reported consuming four meals a day. Lunch was the most reported meal, followed by breakfast. A total of 30.6% of men and 38.6% of women had adequate frequency of meals (p=0.002). Men with edentulism (total tooth loss) were 35% more likely to eat adequately, while this probability was about 30% lower among non-white women, those without schooling and those who reported a lack of money to buy food. One in each three elderly people met the recommendation of adequate frequency of meals and some sociodemographic characteristics were negatively associated with this habit.

O objetivo do estudo foi descrever a frequência e tipos de refeições realizadas por idosos de Pelotas-RS e fatores associados à frequência adequada de refeições. Foi realizado estudo transversal com indivíduos ≥60 anos. Questionou-se sobre as refeições diárias na semana anterior à entrevista. A adequação de refeições foi definida conforme recomendação do Ministério da Saúde (pelo menos três refeições principais e dois lanches por dia). As variáveis independentes foram as características sociodemográficas, de saúde e da rotina alimentar. Razões de prevalência e intervalos de confiança de 95% foram calculados por regressão de Poisson. Dos 1.438 idosos entrevistados, cerca de 40% afirmaram realizar quatro refeições diárias. O almoço foi a refeição mais relatada, seguido do café da manhã. Apresentaram frequên cia adequada de refeições 30,6% dos homens e 38,6% das mulheres (p=0,002). Homens com edentulismo (perda dentária total) tiveram 35% maior probabilidade de adequação, enquanto essa probabilidade foi cerca de 30% menor em mulheres de cor da pele não branca, não escolarizadas e que declararam falta de dinheiro para a compra de alimentos. Um a cada três idosos atendeu a recomendação da frequência adequada de refeições e algumas características sociodemográficas foram negativamente associadas com esse hábito.

Adequação da frequência e tipos de refeições realizadas por idosos comunitários do sul do Brasil / Adequacy of the frequency and types of meals consumed by community-dwelling elderly folk in the south of Brazil

Resumo O objetivo do estudo foi descrever a frequência e tipos de refeições realizadas por idosos de Pelotas-RS e fatores associados à frequência adequada de refeições. Foi realizado estudo transversal com indivíduos ≥60 anos. Questionou-se sobre as refeições diárias na semana anterior à entrevista. A adequação de refeições foi definida conforme recomendação do Ministério da Saúde (pelo menos três refeições principais e dois lanches por dia). As variáveis independentes foram as características sociodemográficas, de saúde e da rotina alimentar. Razões de prevalência e intervalos de confiança de 95% foram calculados por regressão de Poisson. Dos 1.438 idosos entrevistados, cerca de 40% afirmaram realizar quatro refeições diárias. O almoço foi a refeição mais relatada, seguido do café da manhã. Apresentaram frequên cia adequada de refeições 30,6% dos homens e 38,6% das mulheres (p=0,002). Homens com edentulismo (perda dentária total) tiveram 35% maior probabilidade de adequação, enquanto essa probabilidade foi cerca de 30% menor em mulheres de cor da pele não branca, não escolarizadas e que declararam falta de dinheiro para a compra de alimentos. Um a cada três idosos atendeu a recomendação da frequência adequada de refeições e algumas características sociodemográficas foram negativamente associadas com esse hábito.

Abstract The scope of this study was to describe the frequency and types of meals consumed by the elderly in Pelotas (Brazil) and factors associated with the adequacy of meal frequency. A cross-sectional study was carried out with ≥60 years of age individuals. They were asked about daily meals during the week prior to the interview. The adequacy of meals was defined as recommended by the Ministry of Health (at least three main meals and two small snacks per day). The independent variables were sociodemographic, health and food routine characteristics. Prevalence ratios and 95% confidence intervals were calculated using Poisson regression. Of the 1,438 elderly individuals interviewed, about 40% reported consuming four meals a day. Lunch was the most reported meal, followed by breakfast. A total of 30.6% of men and 38.6% of women had adequate frequency of meals (p=0.002). Men with edentulism (total tooth loss) were 35% more likely to eat adequately, while this probability was about 30% lower among non-white women, those without schooling and those who reported a lack of money to buy food. One in each three elderly people met the recommendation of adequate frequency of meals and some sociodemographic characteristics were negatively associated with this habit.

Salivary characteristics may be associated with burning mouth syndrome?

BACKGROUND: Burning mouth syndrome (BMS) it is characterized by burning and uncomfortable sensations with no clinical alterations or laboratory findings. The evaluation of the salivary characteristics of people with BMS can help the understanding of the pathogenesis of this condition. This case-control study aimed to trace the salivary profile of women with burning mouth syndrome (BMS). MATERIAL AND METHODS: 40 women with BMS and 40 control women were recruited. Unstimulated salivary flow rate (uSFR), pH, salivary cortisol levels, salivary viscosity, and oral health impact profile (OHIP-14 questioner) were determined. P< 0.05 was considered statistically significant. RESULTS: For uSFR, mean values obtained for BMS and for control group respectively were 0.35 and 0.61 mL/min; for pH, 7.23 and 7.34; for cortisol levels, 0.36 and 0.15 µg/dL; for viscosity values, 31.1 and 45.01 mPas and for OHIP-14 scores, 21.7 and 5.7. To uSFR, cortisol levels, viscosity values and OHIP-14 scores, differences were statistically significant. Salivary cortisol levels and OHIP-14 scores were correlated positively (rho = 0.624; p< 0.05). CONCLUSIONS: BMS women have lower uSFR and salivary viscosity and higher salivary cortisol levels that were associated with worse quality of life, compared with the control group. Key words:Xerostomia, Burning mouth syndrome, Viscosity.