ABSTRACT
BACKGROUND: Alcohol intake is associated with oral diseases and bone changes including alveolar bone loss in humans and in experimental animals. The main aim of the present study is to assess the effect of long-term alcohol intake, at different frequencies, on periodontal bone loss (PBL) in adult rats. MATERIAL AND METHODS: Thirty-six (36) rats were divided into 3 groups: Control (daily water intake, n=12), daily alcohol intake (20% ethanol, n=12), and social alcohol intake (20% ethanol twice a week, n=12). The rats were sacrificed after 90 days and their right maxillae were removed. Initially, a random portion from each group was analyzed through SEM (scanning electron microscope) to assess surface topography. Next, all pieces were dissected and stained with methylene blue 1% and photographed in stereomicroscope at 10x magnification. The PBL was assessed by measuring the distance between cement-enamel junction and alveolar bone crest. RESULTS: Results showed higher (p=0.0368) alcohol solution amount in the daily intake group than in the twice week intake one. The SEM showed qualitatively flat bone surface in the control group, the social intake group presented surface with few minor hollows, and the daily intake group evidenced increased number and diameter of wells. The comparison between groups showed higher bone loss (p<0.05) in both frequencies than in the control, but the bone loss was lower (p<0.05) in the social alcohol intake group than in the daily intake one. CONCLUSIONS: Alcohol intake may cause alveolar bone loss in periodontitis-free rats depending on the frequency. Key words:Alcohol intake, alveolar bone loss, alcohol-induced periodontitis, alcoholic rats.
ABSTRACT
Objectives: The aim of the present study was to assess the effect of regular and constant longterm alcohol consumption on the percentage of the remaining periodontal bone support (PBS) and periodontal bone loss (PBL) in adult rats. Material and Methods: Fifty-four (54) rats were divided into 3 groups: Control (daily water intake, n=18) daily alcohol intake (20% ethanol, n=18) and social alcohol intake (20% ethanol 2x a week, n=18). The rats were treated with free-choice access to both ethanol consumption frequencies. They were euthanized after 90 days and their left mandibles were radiographed for PBS measuring. The same left mandibles were defleshed and stained. The PBL was morphometrically assessed by measuring the distance between cement-enamel junction and alveolar bone crest. Results: Did not show difference (p > 0.05) in the amount of consumed alcohol between the social and daily intake groups. Rats also evidenced lower PBS percentage and higher PBL (p<0.05) in both alcohol consumption groups in comparison to the control. Conclusion: The long-term constant and regular same amount alcohol consumption may cause alveolar bone loss and reduce the remaining periodontal bone support in adult rats. Thus, the alveolar bone loss was associated with the amount of consumed alcohol, rather than with periodicity in periodontitis-free rats (AU)
Objetivo: O objetivo do presente estudo foi avaliar o efeito do consumo regular e constante de álcool em longo prazo na porcentagem do suporte ósseo periodontal remanescente (SOP) e perda óssea periodontal (PO) em ratos adultos. Material e Métodos: Cinquenta e quatro (54) ratos foram divididos em 3 grupos: Controle (ingestão diária de água, n = 18), consumo diário de álcool (20% etanol, n = 18) e consumo social de álcool (20% etanol 2x por semana, n = 18). Os ratos foram tratados com acesso de livre escolha a ambas as frequências de consumo de etanol. Eles foram sacrificados após 90 dias e suas mandíbulas esquerdas foram radiografadas para medição do SOP. As mesmas mandíbulas esquerdas foram dissecadas e coradas. A PO foi avaliada morfometricamente medindo a distância entre a junção cimento-esmalte e a crista óssea alveolar. Resultados: Não houve diferença (p> 0,05) na quantidade de álcool consumido entre os grupos de ingestão diária e social. Os ratos também evidenciaram menor porcentagem de SOP e maior PO (p<0,05) em ambos os grupos de consumo de álcool em comparação com o controle. Conclusão: A mesma quantidade consumida constante e regular de álcool em longo prazo pode causar perda óssea alveolar e reduzir o suporte ósseo periodontal remanescente em ratos adultos. Assim, a perda óssea alveolar foi associada com a quantidade de álcool consumido, ao invés da periodicidade em ratos sem periodontite. (AU)