Subject(s)
Dog Diseases/epidemiology , Dog Diseases/microbiology , Ehrlichia/isolation & purification , Ehrlichiosis/veterinary , Animals , Blood/microbiology , Brazil/epidemiology , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , Dogs , Ehrlichia/genetics , Ehrlichiosis/epidemiology , Ehrlichiosis/microbiology , Incidence , Polymerase Chain ReactionSubject(s)
Cat Diseases/microbiology , Ehrlichia canis/isolation & purification , Ehrlichiosis/veterinary , Animals , Blood/microbiology , Brazil , Cats , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Ehrlichiosis/microbiology , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNASubject(s)
Antibodies, Bacterial/blood , Rickettsia Infections/veterinary , Rickettsia/immunology , Animals , Animals, Domestic , Animals, Wild , Brazil/epidemiology , Fluorescent Antibody Technique, Indirect , Rickettsia Infections/epidemiology , Rickettsia Infections/immunology , Seroepidemiologic StudiesABSTRACT
Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17 kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.
Subject(s)
Animals, Domestic/parasitology , DNA, Bacterial/genetics , Insect Vectors/microbiology , Rickettsia felis/genetics , Siphonaptera/microbiology , Ticks/microbiology , Animals , Brazil/epidemiology , DNA, Bacterial/isolation & purification , Dogs , Endemic Diseases , Environment , Horses , Humans , Insect Vectors/classification , Polymerase Chain Reaction , Rickettsia Infections/epidemiology , Rickettsia Infections/microbiology , Rickettsia Infections/transmission , Rickettsia felis/isolation & purification , Siphonaptera/classification , Ticks/classificationABSTRACT
Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.
Subject(s)
Animals , Dogs , Humans , Animals, Domestic/parasitology , DNA, Bacterial/genetics , Siphonaptera/microbiology , Insect Vectors/microbiology , Rickettsia felis/genetics , Ticks/microbiology , Brazil/epidemiology , DNA, Bacterial/isolation & purification , Endemic Diseases , Environment , Siphonaptera/classification , Horses , Insect Vectors/classification , Polymerase Chain Reaction , Rickettsia Infections/epidemiology , Rickettsia Infections/microbiology , Rickettsia Infections/transmission , Rickettsia felis/isolation & purification , Ticks/classificationABSTRACT
The authors describe their work in the Americas in Rickettsia felis cases in humans and the presence of Rickettsia felis in vectors.
Subject(s)
Rickettsia Infections/epidemiology , Rickettsia felis , Animals , Humans , Insect Vectors , North America/epidemiology , Rickettsia Infections/diagnosis , South America/epidemiologyABSTRACT
We revisited a Brazilian spotted fever focal area in Minas Gerais state, Brazil, in 2002, and performed a serologic survey in dogs and cats. The results of this survey are compared with the survey made 10 years before. The possible efficacy of vector control measures adopted in this area and the role of dogs and horses as sentinels of infection by Rickettsia are discussed.
Subject(s)
Rickettsia Infections/epidemiology , Animals , Brazil/epidemiology , Health Surveys , Horses/microbiology , Humans , Incidence , Rickettsia Infections/transmission , Ticks/microbiology , ZoonosesABSTRACT
The authors describe cases of Brazilian spotted fever (BSF) diagnosed in Minas Gerais state, Brazil, by the Minas Gerais Public Health Laboratory, Ezequiel Dias Foundation from 1995 to 2004. In addition they present three cases of human Rickettsia felis rickettsiosis from Minas Gerais diagnosed in France in 1999, and the first two suspected cases of human monocytic ehrlichiosis (HME) diagnosed in Ezequiel Dias Foundation in 2001. In both cases a differential diagnose was made with BSF.