ABSTRACT
The power ultrasonic generator (PUG) is the core device of power ultrasonic technology (PUT), and its performance determines the application of this technology in biomedicine, semiconductor, aerospace, and other fields. With the high demand for sensitive and accurate dynamic response in power ultrasonic applications, the design of PUG has become a hot topic in academic and industry. However, the previous reviews cannot be used as a universal technical manual for industrial applications. There are many technical difficulties in establishing a mature production system, which hinder the large-scale application of PUG for piezoelectric transducers. To enhance the performance of the dynamic matching and power control of PUG, the studies in various PUT applications have been reviewed in this article. Initially, the demand design covering the piezoelectric transducer application and parameter requirements for ultrasonic and electrical signals is overall summarized, and these parameter requirements have been recommended as the technical indicators of developing the new PUG. Then the factors affecting the power conversion circuit design are analyzed systematically to realize the foundational performance improvement of PUG. Furthermore, advantages and limitations of key control technologies have been summarized to provide some different ideas on how to realize automatic resonance tracking and adaptive power adjustment, and to optimize the power control and dynamic matching control. Finally, several research directions of PUG in the future have been prospected.
ABSTRACT
A rhabditid entomopathogenic nematode (EPN), Oscheius chongmingensis, has a stable symbiotic relationship with the bacterial strain Serratia nematodiphila S1 harbored in its intestines and drastically reduced viability when associated with a non-native strain (186) of the same bacterial species. This nematode is thus a good model for understanding the molecular mechanisms and interactions involved between a nematode host and a member of its intestinal microbiome. Transcriptome analysis and RNA-seq data indicated that expression levels of the majority (8797, 87.59%) of mRNAs in the non-native combination of O. chongmingensis and S. nematodiphila 186 were downregulated compared with the native combination, including strain S1. Accordingly, 88.84% of the total uniq-sRNAs mapped in the O. chongmingensis transcriptome were specific between the two combinations. Six DEGs, including two transcription factors (oc-daf-16 and oc-goa-1) and four kinases (oc-pdk-1, oc-akt-1, oc-rtk, and oc-fak), as well as an up-regulated micro-RNA, oc-miR-71, were found to demonstrate the regulatory mechanisms underlying diminished host viability induced by a non-native bacterial strain. Oc-rtk and oc-fak play key roles in the viability regulation of O. chongmingensis by positively mediating the expression of oc-daf-16 to indirectly impact its longevity and stress tolerances and by negatively regulating the expression of oc-goa-1 to affect the olfactory chemotaxis and fecundity. In response to the stress of invasion by the non-native strain, the expression of oc-miR-71 in the non-native combination was upregulated to downregulate the expression of its targeting oc-pdk-1, which might improve the localization and activation of the transcription factor DAF-16 in the nucleus to induce longevity extension and stress resistance enhancement to some extent. Our findings provide novel insight into comprehension of how nematodes deal with the stress of encountering novel potential bacterial symbionts at the physiological and molecular genetic levels and contribute to improved understanding of host-symbiont relationships generally.
Subject(s)
MicroRNAs , Nematoda , Animals , Sequence Analysis, DNA , Symbiosis , Nematoda/physiology , IntestinesABSTRACT
This study used a forming grinding wheel to machine an involute spur gear with ultrasonic vibration applied to the gear in order to improve the gear processing technology and enhance the gear processing effect. Conventional grinding and ultrasonic vibration-assisted forming grinding gear (TUVA-FGG) tests were carried out. The effects of grinding parameters, such as spindle speed, feed rate, radial grinding depth, and ultrasonic amplitude, on grinding force, grinding temperature, residual stress, surface roughness, and surface morphology were analyzed. The TUVA-FGG significantly improved processing efficiency. With the increase in spindle speed, the maximum reductions in the normal and tangential grinding forces, grinding temperature, and surface roughness reached 33.6, 24.5, 23.9, and 21.6%, respectively. With the increase in feed rate, the respective maximum reductions were 21.4, 19.7, 20.3, and 16.1%. With the increase in radial grinding depth, these values attained 24.6, 20.3, 21.5, and 17.6%, respectively. Finally, with the increase in ultrasonic amplitude, these reductions reached 21.4, 19.7, 21.5, and 19.4%. The maximum residual compressive stress grew by 30.3, 27.5, 30.9, and 27.5% with the increase of wheel rotation speed, feed speed, radial grinding depth, and ultrasonic amplitude, respectively. TUVA-FGG changed the conventional continuous cutting mechanism between the abrasive grain and workpiece into intermittent cutting, reducing grinding forces, grinding temperature, and surface roughness. Moreover, it increased residual compressive stress and improved the micromorphology of the tooth surface, thus enhancing gear machining efficiency.
ABSTRACT
In this study, we propose a longitudinal-torsion ultrasonic-assisted milling (LTUM) machining method for difficult-to-cut materials-such as titanium alloy-in order to realize anti-fatigue manufacturing. In addition, a theoretical prediction model of cutting force is established. To achieve this, we used the cutting edge trajectory of LTUM to reveal the difference in trajectory between LTUM and traditional milling (TM). Then, an undeformed chip thickness (UCT) model of LTUM was constructed. From this, the cutting force model was able to be established. A series of experiments were subsequently carried out to verify this LTUM cutting force model. Based on the established model, the influence of several parameters on cutting force was analyzed. The results showed that the established theoretical model of cutting force was in agreement with the experimental results, and that, compared to TM, the cutting force was lower in LTUM. Specifically, the cutting force in the feed direction, Fx, decreased by 24.8%, while the cutting force in the width of cut direction Fy, decreased by 29.9%.
ABSTRACT
Cuticular wax covers aerial organs of plants and functions as the outermost barrier against non-stomatal water loss. We reported here the functional characterization of the Glossy1(GL1)-homologous gene OsGL1-3 in rice using overexpression and RNAi transgenic rice plants. OsGL1-3 gene was ubiquitously expressed at different level in rice plants except root and its expression was up-regulated under ABA and PEG treatments. The transient expression of OsGL1-3-GFP fusion protein indicated that OsGL1-3 is mainly localized in the plasma membrane. Compared to the wild type, overexpression rice plants exhibited stunted growth, more wax crystallization on leaf surface, and significantly increased total cuticular wax load due to the prominent changes of C30-C32 aldehydes and C30 primary alcohols. While the RNAi knockdown mutant of OsGL1-3 exhibited no significant difference in plant height, but less wax crystallization and decreased total cuticular wax accumulation on leaf surface. All these evidences, together with the effects of OsGL1-3 on the expression of some wax synthesis related genes, suggest that OsGL1-3 is involved in cuticular wax biosynthesis. Overexpression of OsGL1-3 decreased chlorophyll leaching and water loss rate whereas increased tolerance to water deficit at both seedling and late-tillering stages, suggesting an important role of OsGL1-3 in drought tolerance.
Subject(s)
Adaptation, Physiological , Membrane Proteins/metabolism , Oryza/metabolism , Plant Proteins/metabolism , Water/metabolism , Waxes/metabolism , Abscisic Acid/pharmacology , Cell Membrane Permeability , Chlorophyll/metabolism , Droughts , Gene Expression Regulation, Plant/drug effects , Membrane Proteins/genetics , Microscopy, Electron, Scanning , Oryza/genetics , Phylogeny , Plant Epidermis/genetics , Plant Epidermis/metabolism , Plant Growth Regulators/pharmacology , Plant Leaves/genetics , Plant Leaves/metabolism , Plant Leaves/ultrastructure , Plant Proteins/classification , Plant Proteins/genetics , Plants, Genetically Modified , RNA Interference , Reverse Transcriptase Polymerase Chain Reaction , Seedlings/genetics , Seedlings/metabolismABSTRACT
Expression profiles of nine rice heat shock protein genes (OsHSPs) were analyzed by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The nine genes exhibited distinctive expression in different organs. Expression of nine OsHSP genes was affected differentially by abiotic stresses and abscisic acid (ABA). All nine OsHSP genes were induced strongly by heat shock treatment, whereas none of them were induced by cold. The transcripts of OsHSP80.2, OsHSP71.1 and OsHSP23.7 were increased during salt tress treatment. Expression of OsHSP80.2 and OsHSP24.1 genes were enhanced while treated with 10% PEG. Only OsHSP71.1 was induced by ABA while OsHSP24.1 was suppressed by ABA. These observations imply that the nine OsHSP genes may play different roles in plant development and abiotic stress responses.
Subject(s)
Abscisic Acid/pharmacology , Gene Expression Regulation, Plant/drug effects , Genes, Plant , Heat-Shock Proteins/genetics , Oryza/drug effects , Oryza/genetics , Stress, Physiological/drug effects , Gene Expression Profiling , Heat-Shock Proteins/classification , Heat-Shock Proteins/metabolism , Organ Specificity/drug effects , Organ Specificity/genetics , Phylogeny , Polyethylene Glycols/pharmacology , Sodium Chloride/pharmacology , Stress, Physiological/geneticsABSTRACT
During a recent soil sample survey in Eastern China, a new entomopathogenic nematode species, collected from the Chongming Islands in the southern-eastern area of Shanghai, was discovered. Morphological characteristics of different developmental stages of the nematode combined with molecular data showed that this nematode is a new genus of Rhabditidae, and described as Heterorhabditidoides chongmingensis gen. nov., sp. nov., for that it shares more morphological characteristics with heterorhabditids than with steinernematids. For males, the papillae formula of bursa is 1, 2, 3, 3, with constant papillae number in the terminal group, stoma tubular-shaped and about 1.5 head width; cheilorhabdions cuticularized, esophageal collar present and long, median bulb present. For infective juveniles, EP=90 (80-105)microm, ES=104 (92-120)microm, tail length=111 (89-159)microm, and a=19.1 (15-21). The percentages of the nucleotides A, T, C and G in the ITS1 regions of the new species are significantly different from those of heterorhabditids and other rhabditids. Molecular phylogenetic trees based on 18S rDNA and the internal transcribed spacer (ITS) sequences data revealed that the new entomopathogenic nematode species forms a monophyletic group, which is a sister group of the clade comprised of some genera of Rhabditidae.
Subject(s)
Rhabditida/anatomy & histology , Rhabditida/classification , Rhabditida/physiology , Animals , Base Sequence , DNA, Helminth , Female , Host-Pathogen Interactions/physiology , Male , Microscopy, Electron, Scanning , Microscopy, Fluorescence , Molecular Sequence Data , Phylogeny , Polymerase Chain ReactionABSTRACT
Esophageal cancer is a leading cause of cancer death, especially in developing countries. In high-risk regions, squamous cell carcinoma is the most common type of esophageal cancer, and its etiology remains poorly understood. The purpose of this study was to evaluate the association between human papillomavirus (HPV) infection and esophageal squamous cell carcinoma (ESCC) and related precursor lesions in a high-risk area of China. We conducted a cross-sectional study among adult inhabitants of Linxian, China. All subjects were interviewed about potential risk factors, had the length of their esophagus sampled by a balloon cytology examination and underwent endoscopy with mucosal iodine staining and biopsy of all unstained lesions. A multivalent HPV hybridization probe, Digene Hybrid Capture II (Gaithersburg, MD), which recognizes high-risk types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68, was used to determine the HPV infection status of the cytologic specimens, and the endoscopic biopsies were used to classify each subject's esophageal disease. 740 subjects completed the cytologic and endoscopic exams, and 702 had adequate cytologic and biopsy specimens. Using a cutpoint of > or =3.0 pg/ml of HPV DNA to define a positive test, HPV positivity was identified in 13% (61/475) of subjects without squamous dysplasia, 8% (8/102) with mild dysplasia, 7% (6/83) with moderate dysplasia, 16% (6/38) with severe dysplasia and zero (0/4) with invasive ESCC. Changing the cutpoint defining a positive test did not change the association of HPV infection and dysplasia grade. In this high-risk population, infection of esophageal cells with high-risk HPV types occurs in 13% of asymptomatic adults with no evidence of squamous dysplasia and a similar proportion of individuals with mild, moderate or severe dysplasia. This suggests that HPV infection is not a major risk factor for ESCC in this high-risk Chinese population. Further studies are warranted to determine if infection with this agent is associated with neoplastic progression in a subset of cases.
Subject(s)
Carcinoma, Squamous Cell/virology , Esophageal Neoplasms/virology , Papillomaviridae/isolation & purification , Papillomavirus Infections/virology , Adult , Carcinoma, Squamous Cell/epidemiology , China/epidemiology , Cross-Sectional Studies , DNA Probes, HPV , DNA, Viral/analysis , Disease Progression , Esophageal Neoplasms/epidemiology , Female , Humans , Male , Middle Aged , Papillomavirus Infections/epidemiologyABSTRACT
BACKGROUND: To establish a cohort for the study of risk factors of lung cancer, and to support the study of early biomarkers and prevention of lung cancer. METHODS: Designed a special population-based prospectively dynamic cohort among radon- and arsenic-exposed tin miners aged 40 or more years old with at least 10 years of occupational exposure in Yunnan Province, P.R.China. The mass screenings with sputum cytology and chest X-ray were conducted annually. The baseline information was collected for assessing demographic characteristics and risk factors. The multiple sputum specimens, chest radiographs and numerous biologic specimens have been collected and stored. RESULTS: From 1992-1999, 9143 miners have been enrolled and 460 new cases of lung cancer have been found. There had 47655 person-time chest radiographs and 46625 person-time sputum cytology among the cohort in 8 years. The relative risks of age-adjusted exposure to chronic bronchitis, silicosis, and tobacco were 1.73, 1.46, and 1.32 respectively. CONCLUSIONS: A cohort of unique occupationally-exposed tin miners with an extensive biologic specimen repository and data bank has been successfully established. Although occupational exposures are the predominant risk factors among the high risk miners, lung cancer risk is also associated with chronic obstructive lung disease (chronic bronchitis and silicosis) and a number of measures of exposure to tobacco smoke, including early age of first use, duration, and cumulative exposure.
ABSTRACT
OBJECTIVE: To analyze the effect of gold standard, blind comparison and different cut-points choosing on screening techniques assessment, and to promote the application of evidence-based medicine theory in screening study. METHODS: A screening study for cervical cancer in rural China in 1999, where 1997 women had been tested for pathology as gold standard and simulating situations without gold standard, blind comparison and under different cut-points. Indices such as detectable rate, sensitivity and specificity were calculated for each technique. Receiver operating characteristics (ROC) curves were drawn and areas under ROC curves between screening techniques were tested. RESULTS: Without gold standard, diagnostic techniques could not be evaluated correctly, and without the blind comparison, the sensitivity and specificity of the tests would be subjectively increased. Furthermore, use of different cut-points led to different sensitivities and specificities of test. CONCLUSIONS: Gold standard, blind comparison and perfect cut-points can improve the quality of screening test and drawing ROC curves is an effective way to confirm cut-points and evaluate diagnostic techniques. It is necessary to enforce the application of evidence-based medicine theory in scientific research.