[Longitudinal surveillance of intestinal nematodiasis in Yinghu Village of Wujiang City].

OBJECTIVE: To understand the epidemic dynamics of intestinal nematodiasis in Wujiang City. METHOD: The residents of Yinghu Village of Wujiang City were investigated with Kato-Katz technique for the infections of intestinal nematodes, and the persons with the infections were administered with anthelmintics and they also received the health education. RESULTS: Of the 5 757 residents, 147 persons were infected with intestinal nematodes, with the average infection rate of 2.55%. The infection rates of hookworm, Ascaris lumbricoides and Tricuris trichiura were 1.96%, 0.49% and 0.24%, respectively. The repeated infection rate was 20.30%, that was 10.36 times higher than the new infection rate (1.96%). CONCLUSIONS: There are still regions and populations with high infection rates of intestinal nematodes in Wujiang City where the intestinal nematodiasis has been controlled. Therefore, it is necessary to strengthen the monitoring, prevention and control work.

[T lymphocytes with chimeric receptor induce carcinoembryonic antigen-positive specific gastric carcinoma cells apoptosis].

OBJECTIVE: To develop a method to generate T lymphocytes that identify tumor specific carcinoembryonic antigen (CEA), and induce anti-tumor response such as apoptosis. METHODS: Peripheral blood samples were collected from 10 healthy persons and peripheral blood mononuclear cells (PBMCs) were isolated. 1, then CD8(+) T cells were isolated from the PBMCs by magnetic activated cell sorting (MACS) using magnetic beads-conjugated anti-CD8 monoclonal antibodies. Then the recombinant vector anti-CEA-scFv-CD3zeta-pcDNA3.0 was transfected into the CD8(+) T cells by lipofectamine 2000 and T lymphocytes with chimeric receptor were generated and cultivated. The T lymphocytes' activation was assessed by detecting the expression of CD69, an early signal of T cell activation. Human gastric carcinoma cells of the lines HGC-27 (CEA(+)) and SGC-7901 (CEA(-)) were cocultivated with the T lymphocytes with chimeric receptor. Flow cytometry (FCM) with annexin V-FITC/PI double staining was used to detect the expressions of annexin V, a signal of cell apoptosis, so as to observe the apoptosis of the gastric carcinoma cells. T cells activated by phytohemagglutinin (PHA) were used as positive controls, and T cells transfected with blank vector pcDNA3.0 were used as negative controls. RESULTS: T lymphocytes with chimeric receptor directed towards CEA(+) gastric carcinoma cells were generated. 12 and 24 hours after the co-culture of these T cells and HCG-27 cells, the CD69 expression rates were 40.5% +/- 3.4% and 48.3% +/- 2.8% respectively. However, the CD69 expression rates of the HGC-27 and SGC-7901 cells transfected with the T lymphocytes transfected with blank vector were both 0%. FCM showed that the apoptotic rates of the CEA(+) gastric tumor HGC-27 cells was 47.8% +/- 4.2%, significantly higher than that of the CEA(-) gastric tumor SGC-7901 cells (18.7% +/- 2.8%, P < 0.05). CONCLUSION: T lymphocytes with chimeric receptor targeting CEA specifically identify CEA positive gastric carcinoma cells and promote the apoptosis thereof, thus providing a promising method of cellular immunotherapy for gastric carcinomas.