A gain-of-function mutation in BnaIAA13 disrupts vascular tissue and lateral root development in Brassica napus.

Rapeseed (Brassica napus) is an important oilseed crop worldwide. Plant vascular tissues are responsible for material transport and provide mechanical support. The lateral roots (LRs) absorb sufficient water and nutrients. The genetic basis of vascular tissues and LRs development in rapeseed remains unknown. This study characterized an EMS-mutagenized rapeseed mutant, T16, which showed dwarf stature, reduced LRs, and leaf wilting. Scanning electron microscopy observations showed that the internode-cell shortened. Observations of the tissue sections revealed defects in the development of vascular bundles in the stems and petioles. Genetic analysis revealed that the phenotypes of T16 were controlled by a single semi-dominant nuclear gene. Map-based cloning and genetic complementarity confirmed that BnaA03.IAA13 is the functional gene, a G-to-A mutation in second exon changed the glycine at the 79th position to glutamic acid, disrupting the conserved degron motif VGWPP. Transcriptome analysis in roots and stems showed that auxin and cytokinin signaling pathways were disordered in T16. Evolutionary analysis showed that AUXIN/INDOLE-3-ACETIC ACID was conserved during plant evolution. The heterozygote of T16 significantly reduced the plant height while maintaining other agronomic traits. Our findings provide novel insights into the regulatory mechanisms of vascular tissues and LRs development, and provide a new germplasm resource for rapeseed breeding.

Activation of Peracetic Acid by CoFe2O4 for Efficient Degradation of Ofloxacin: Reactive Species and Mechanism.

Peroxyacetic acid (PAA)-based advanced oxidation processes (AOPs) have attracted much attention in wastewater treatment by reason of high selectivity, long half-life reactive oxygen species (ROS), and wider applicability. In this study, cobalt ferrite (CoFe2O4) was applied to activate PAA for the removal of ofloxacin (OFX). The degradation of OFX could reach 83.0% via the CoFe2O4/PAA system under neutral conditions. The low concentration of co-existing anions and organic matter displayed negligible influence on OFX removal. The contributions of hydroxyl radicals (·OH), organic radicals (R-O·), and other reactive species to OFX degradation in CoFe2O4/PAA were systematically evaluated. Organic radicals (especially CH3C(O)OO·) and singlet oxygen (1O2) were verified to be the main reactive species leading to OFX destruction. The Co(II)/Co(III) redox cycle occurring on the surface of CoFe2O4 played a significant role in PAA activation. The catalytic performance of CoFe2O4 remained above 80% after five cycles. Furthermore, the ecotoxicity of OFX was reduced after treatment with the CoFe2O4/PAA system. This study will facilitate further research and development of the CoFe2O4/PAA system as a new strategy for wastewater treatment.

Mechanistic Study of the Transition from Antimony Oxide to Antimony Sulfide in the Hydrothermal Process to Obtain Highly Efficient Solar Cells.

Obtaining high-quality absorber layers is a major task for constructing efficient thin-film solar cells. Hydrothermal deposition is considered a promising method for preparing high-quality antimony sulfide (Sb2 S3 ) films for solar cell applications. In the hydrothermal process, the precursor reactants play an important role in controlling the film formation process and thus the film quality. In this study, Sb2 O3 is applied as a new Sb source to replace the traditional antimony potassium tartrate to modulate the growth process of the Sb2 S3 film. The reaction mechanism of the transition from Sb2 O3 to Sb2 S3 in the hydrothermal process is revealed. Through controlling the nucleation and deposition processes, high-quality Sb2 S3 films are prepared with longer carrier lifetimes and lower deep-level defect densities than those prepared from the traditional Sb source of antimony potassium tartrate. Consequently, a solar cell device based on this improved Sb2 S3 delivers a high power conversion efficiency of 6.51 %, which is in the top tier for Sb2 S3 -based solar devices using hydrothermal methods. This research provides a new and competitive Sb source for hydrothermal growth of high-quality antimony chalcogenide films for solar cell applications.

BnaCRCs with domestication preference positively correlate with the seed-setting rate of canola.

Canola (Brassica napus) is an important oil crop worldwide. The seed-setting rate (SS) is a critical factor in determining its yield, and the development of pistils affects pollination and seed sets. However, research on seed-setting defects has been limited owing to difficulties in the identification of phenotypes, mutations, and complex genetic mechanisms. In this study, we found a stigma defect (sd) mutant in B. napus, which had no nectary. The SS of sd mutants in the field was approximately 93.4% lower than that of the wild type. Scanning and transmission electron microscopy imaging of sd mutants showed a low density of stigma papillary cells and stigma papillary cell vacuoles that disappeared 16 h after flowering. Genetic analysis of segregated populations showed that two recessive nuclear genes are responsible for the mutant phenotype of sd. Based on re-sequencing and map-based cloning, we reduced the candidate sites on ChrA07 (BnaSSA07) and ChrC06 (BnaSSC06) to 30 and 67 kb, including six and eight predicted genes, respectively. Gene analyses showed that a pair of CRABS CLAW (CRC) homeologous genes at BnaSSA07 and BnaSSC06 were associated with the development of carpel and nectary. BnaSSA07.CRC and BnaSSC06.CRC candidate genes were found to be expressed in flower organs only, with significant differences in their expression in the pistils of the near-isogenic lines. DNA sequencing showed transposon insertions in the upstream region and intron of the candidate gene BnaSSA07.crc. We also found that BnaSSC06.crc exists widely in the natural population and we give possible reasons for its widespread existence.

Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.

A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel is explored via UV-polymerization. This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.

An Ion-Crosslinked Supramolecular Hydrogel for Ultrahigh and Fast Uranium Recovery from Seawater.

Large-scale uranium extraction from seawater is a crucial but challenging part of nuclear power generation. In this study, a new ion-crosslinked supramolecular Zn2+ -poly(amidoxime) (PAO) hydrogel that can super-efficiently adsorb uranium from seawater is explored. By simply mixing two solutions of zinc chloride and PAO, a supramolecular Zn2+ -PAO hydrogel is achieved via the interaction between zinc cations and amidoxime anions. In contrast with existing amidoxime-functionalized hydrogel-based adsorbents having low PAO contents and fiber-based adsorbents with weak hydrophilicity, the PAOs can be directly crosslinked using a small quantity of superhydrophilic zinc ion. Thus, a supramolecular hydrogel is formed, having both a high content of well-dispersed PAOs and good hydrophilicity. Relative to reported adsorbents, this low-cost hydrogel membrane exhibits outstanding uranium adsorption performance, reaching 1188 mg g-1 of MU /Mdry gel in 32 ppm uranium-spiked water. More importantly, after immersion in natural seawater for only 4 weeks, the uranium extraction capacity of the Zn2+ -PAO hydrogel membrane reaches 9.23 mg g-1 of MU /Mdry gel . This work can provide a general strategy for designing a new type of supramolecular hydrogel, crosslinked by various bivalent/multivalent cation-crosslinkers and even many other superhydrophilic supramolecular crosslinkers, for the high-efficient and massive extraction of uranium from seawater.

Identification and fine mapping of a major locus controlling branching in Brassica napus.

KEY MESSAGE: A candidate branching-controlling gene for qDBA09 was identified after delimiting a Brassica napus recessive locus within a 270-kb interval on chromosome A09. Although branching is an important trait associated with the adaptation and yield potential of rapeseed (Brassica napus), the genetic mechanisms underlining branching in this crop remain poorly understood. In this study, we characterized a naturally occurring rapeseed mutant, db1, which showed an ultrahigh branching density phenotype. By combining bulked segregant analysis (BSA) and the Brassica 60K SNP BeadChip Array, we identified two major quantitative trait loci (QTLs), qDBA09 and qDBC06, which were subsequently confirmed using the traditional QTL-mapping approach. Analysis of 208 individuals from a BC1F3 population indicated that the qDBA09 locus is a single Mendelian factor and that the dense branching phenotype is controlled by a single recessive gene. Furthermore, QTL analysis confirmed that qDBA09 explained between 9.5 and 70.5% of the variation in branching-related traits. Using 7785 individuals from the BC1F3 population, we mapped qDBA09 to a DNA fragment of approximately 270 kb in length that contained 27 predicted genes, three of which were identified as potentially involved in the control of the dense branching trait. Based on the reported function of these genes, together with sequence comparisons and co-segregation analysis, we identified a potential candidate gene for the qDBA09 locus. The present findings lay the foundations for further in-depth research on the branching mechanisms of B. napus.

Sunlight Polymerization of Poly(amidoxime) Hydrogel Membrane for Enhanced Uranium Extraction from Seawater.

The uranium level in seawater is ≈1000 times as high as terrestrial ores and can provide potential near-infinite fuel for the nuclear energy industry. However, it is still a significant challenge to develop high-efficiency and low-cost adsorbents for massively extracting uranium from seawater. Herein, a simple and fast method through low-energy consumption sunlight polymerization to direct fabrication of a poly(amidoxime) (PAO) hydrogel membrane, which exhibits high uranium adsorption capacity, is reported. This PAO hydrogel owns semi-interpenetrating structure and a hydrophilic poly(acrylamide) 3D network of hydrogel which can disperse and fix PAOs well. As a result, the amidoxime groups of PAOs exhibit an outstanding uranium adsorption efficiency (718 ± 16.6 and 1279 ± 14.5 mg g-1 of m uranium/m PAO in 8 and 32 ppm uranium-spiked seawater, respectively) among reported hydrogel-based adsorbents. Most importantly, U-uptake capacity of this hydrogel can achieve 4.87 ± 0.38 mg g-1 of m uranium/m dry gel just after four weeks within natural seawater. Furthermore, this hydrogel can be massively produced through low-energy consumption and environmentally-friendly sunlight polymerization. This work will provide a high-efficiency and low-cost adsorbent for massive uranium extraction from seawater.

Association of fibronectin Msp iv polymorphism and diabetic nephropathy susceptibility in Chinese Han population.

AIM: Our study was aimed to study the distributional characteristics of fibronectin (Fn) Msp iv polymorphism in Chinese Han Population and investigate its association with susceptibility and clinicopathologic features of diabetic nephropathy (DN). METHODS: Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were applied to testify Fn Msp iv genotypes among 108 patients with DN and 86 healthy individuals. Odds ratio (OR) with 95% confidence interval (CI) were used to evaluate the association of Fn Msp iv polymorphism and onset risk and clinicopathologic stages of DN. RESULTS: The comparison of genotype and allele distribution in normal, micro and massive proteinuria groups showed that genotype and allele distribution in massive proteinuria group showed great differences, compared with those of control group (P=0.006, P=0.004). Further analysis on the association of Fn Msp iv polymorphism and occurrence of abnormal proteinuria suggested that DD genotype and D allele appeared to be a risk factor for abnormal proteinuria (OR=3.553, 95% CI=1.278-9.875; OR=2.442, 95% CI=1.378-4.327). Then, we analyzed the effects of Fn Msp iv polymorphism on the clinicopathologic stages of DN, the result showed that DD genotype showed great effect on the occurrence of early-onset DN (OR=7.500, 95% CI=1.691-33.272). For the DN patients with D allele, the risk for early-onset DN was increased 3.445 folds (OR=4.445, 95% CI=1.869-33.10.574). CONCLUSION: Fn Msp iv polymorphism appeared to be associated with DN susceptibility.

Antiproliferative effects of selective cyclooxygenase-2 inhibitor modulated by nimotuzumab in estrogen-dependent breast cancer cells.

Breast cancer is the most common malignancy in women, and many breast cancer patients fail conventional treatment strategies of chemotherapy, radiation, and antiestrogen therapy. Research into the molecular pathways and biomarkers involved in the development of breast cancer should yield information that will guide therapeutic decisions. Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) are involved in the carcinogenesis of breast cancer and exist tight crosstalk with estrogen receptor (ER) pathway. Combination of EGFR and COX-2 inhibitors, therefore, could be an effective strategy for reducing cell growth in estrogen-dependent breast cancer. In order to verify the effects of EGFR and COX-2 inhibitors, breast cancer cells MCF-7 and SKBR-3 were characterized for receptors status and then treated with respective inhibitors (nimotuzumab and celecoxib) alone and in combination. Both cell lines were sensitive to celecoxib, but not to nimotuzumab. However, combination of two drugs demonstrated synergistic effects on cell killing. Moreover, association of two drugs resulted in SKBR-3 cells, a further G0/G1 phase arrest than one drug alone. Downregulation of p-EGFR, p-Akt, p-mTOR, and amplified in breast cancer 1 (AIB1) were observed in both cell lines, and upregulation of E-cadherin was only found in MCF-7, after treatment with single agent or in combination. These studies suggest that nimotuzumab and celecoxib exert synergistic antiproliferation effects in breast cancer, which partly correlates with ER status. Due to Akt/mTOR, EMT and AIB1 pathways participate in this process, therefore, E-cadherin and AIB1 may be considered as possible biomarkers to predict response in ER-positive breast cancer cells treated with EGFR and COX-2 inhibitors.