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Int J Biol Macromol ; 135: 1020-1027, 2019 Aug 15.
Article in English | MEDLINE | ID: mdl-31152832

ABSTRACT

The effect of the ultrasonic treatment on the physicochemical properties, oil-holding capacities, foaming capacities, and TLR2-affinity of the polysaccharides from Pholiota nameko (PNPS), was evaluated. Compared with the protein content of PNPS before ultrasonic treatment, the protein content of PNPS all presented a decrease under different ultrasonic conditions. The viscosity of PNPS showed a decrease when the ultrasonic intensity was strong enough, as well as the molecular weight. The oil-holding capacity and the foaming capacity of PNPS showed a continuous increasing trend with the increase of the ultrasonic treatment time under a set ultrasonic power of 400 W. Further, the ultrasonic operation could induce the decrease of the affinity binding between PNPS and the receptor proteins TLR2. These data confirmed that applying the ultrasonic treatment could obtain PNPS with high carbohydrate contents, low viscosity and low TLR2-affinity under proper ultrasonic condition.


Subject(s)
Chemical Phenomena , Fungal Polysaccharides/chemistry , Fungal Polysaccharides/isolation & purification , Liquid-Liquid Extraction , Pholiota/chemistry , Toll-Like Receptor 2/chemistry , Ultrasonic Waves , Protein Binding , Rheology , Toll-Like Receptor 2/metabolism
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