ABSTRACT
OBJECTIVE: Based on in vitro and in vivo experimental studies, the changes of the main components of Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated. METHODS: The components of different processed products of Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated. RESULTS: With the extension of processing time, the contents of various chemical components in Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated. Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated. Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated. Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated. CONCLUSION: The content of the main components in Radix Polygonum multiflorum can be affected by processing time; stilbene glycoside may be the main component leading to liver injury. The degree of liver injury caused by Radix Polygonum multiflorum is negatively correlated with processing time.Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated. Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated.
ABSTRACT
Early rehabilitation after surgery for patellar fracture is challenging. The purpose of this study was to evaluate the surgical outcome of titanium cable cerclage for patellar fracture in early functional activity.We reviewed a series of 24 patients treated at our hospital with titanium cable. Functional exercises were started early. Patients were followed up for at least 12 months.Fifteen were males and 9 were females. Fracture occurred in the right knee in 13 patients and in the left knee in 11 patients. The most common mode of injury involves a tumble. None of the patients presented with any postoperative complications. The management resulted in satisfactory outcomes.Titanium cable cerclage offers a new strategy in treating patellar fracture.
Subject(s)
Bone Wires , Fracture Fixation, Internal/instrumentation , Fractures, Bone/surgery , Patella/injuries , Titanium , Adult , Aged , Aged, 80 and over , Female , Follow-Up Studies , Fracture Fixation, Internal/methods , Humans , Male , Middle Aged , Patella/surgery , Retrospective Studies , Treatment Outcome , Young AdultABSTRACT
Kashin-Beck disease (KBD) is a kind of deformity disease involved in cytoskeleton and inner homeostasis regulation. The enrichment analysis of bioprocess, networks, and related disease set were performed. The development regulation, metabolic process, and apoptosis were important procession in KBD; it revealed the up-regulated process in removal of superoxide radicals, glycolysis and glucose catabolic process, regulation of cytoskeleton rearrangement and phagosome in antigen presentation. Morphological changes of KBD chondrocyte were investigated by transmission electronic microscopy compare with the normal one. The ultrastructure of KBD chondrocyte referred to oxidative stress and metabolic dysfunction has been found.
Subject(s)
Chondrocytes/ultrastructure , Cytoskeleton/ultrastructure , Kashin-Beck Disease/metabolism , Kashin-Beck Disease/pathology , Oxidative Stress/physiology , Chondrocytes/metabolism , Cytoskeleton/metabolism , Female , Humans , Male , Microscopy, Electron, Transmission , Middle AgedABSTRACT
OBJECTIVE: To investigate the differences in gene expression profiles of adult articular cartilage from patients with Kashin-Beck disease (KBD) versus those with primary knee osteoarthritis (OA). METHODS: The messenger RNA expression profiles of articular cartilage from patients with KBD, diagnosed according to the clinical criteria for KBD in China, were compared with those of cartilage from patients with OA, diagnosed according to the Western Ontario and McMaster Universities OA Index. Total RNA was isolated separately from 4 pairs of the KBD and OA cartilage samples, and the expression profiles were evaluated by Agilent 4x44k Whole Human Genome density oligonucleotide microarray analysis. The microarray data for selected transcripts were confirmed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) amplification. RESULTS: For 1.2 x 10(4) transcripts, corresponding to 58.4% of the expressed transcripts, 2-fold changes in differential expression were revealed. Expression levels higher in KBD than in OA samples were observed in a mean + or - SD 6,439 + or - 1,041 (14.6 + or - 2.4%) of the transcripts, and expression levels were lower in KBD than in OA samples in 6,147 + or - 1,222 (14.2 + or - 2.8%) of the transcripts. After application of the selection criteria, 1.85% of the differentially expressed genes (P < 0.001 between groups) were detected. These included 233 genes, of which 195 (0.4%) were expressed at higher levels and 38 (0.08%) were expressed at lower levels in KBD than in OA cartilage. Comparisons of the quantitative RT-PCR data supported the validity of our microarray data. CONCLUSION: Differences between KBD and OA cartilage exhibited a similar pattern among all 4 of the pairs examined, indicating the presence of disease mechanisms, mainly chondrocyte matrix metabolism, cartilage degeneration, and apoptosis induction pathways, which contribute to cartilage destruction in KBD.
