ABSTRACT
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Subject(s)
Humans , Female , Aged , Adrenal Cortex Hormones/adverse effects , Aspergillosis/diagnosis , Polymerase Chain Reaction , Adrenal Cortex Hormones/therapeutic use , Celiac Disease/complications , Immunocompetence , Intracranial Hemorrhages/complications , Intracranial Hemorrhages/diagnostic imaging , Lung Diseases, Fungal/diagnosis , Macrophages/drug effects , Aspergillosis/microbiology , Brain Edema/diagnostic imaging , Brain Edema/etiologyABSTRACT
PREMISE OF THE STUDY: Field methodology and image analysis protocols using acoustic tomography were developed and evaluated as a tool to estimate the amount of internal decay and damage of living trees, with special attention to tropical rainforest trees with irregular trunk shapes. METHODS AND RESULTS: Living trunks of a diversity of tree species in tropical rainforests in the Republic of Panama were scanned using an Argus Electronic PiCUS 3 Sonic Tomograph and evaluated for the amount and patterns of internal decay. A protocol using ImageJ analysis software was used to quantify the proportions of intact and compromised wood. The protocols provide replicable estimates of internal decay and cavities for trees of varying shapes, wood density, and bark thickness. CONCLUSIONS: Sonic tomography, coupled with image analysis, provides an efficient, noninvasive approach to evaluate decay patterns and structural integrity of even irregularly shaped living trees.
ABSTRACT
Reduced expression of HLA class I is an important immune escape mechanism from cytotoxic T cells described in various types of malignancy. It often correlates with poor prognosis and resistance to therapy. However, current knowledge about the frequency, underlying molecular mechanisms, and prognostic value of HLA class I and II alterations in prostate cancer (PC) is limited. Immunohistochemical analysis demonstrated that 88 % of the 42 studied cryopreserved prostate tumors have at least one type of HLA alteration as compared to adjacent normal prostate epithelium or benign hyperplasia. Total loss of HLA-I expression found in 50 % of tumors showed an association with increased incidence of tumor relapse, perineural invasion, and high D'Amico risk. The remaining HLA-I-positive tumors demonstrated locus and allelic losses detected in 26 and 12 % of samples, respectively. Loss of heterozygosity at chromosome 6 was detected in 32 % of the studied tumors. Molecular analysis revealed a reduced expression of B2M, TAP2, tapasin and NLRC5 mRNA in microdissected HLA-I-negative tumors. Analysis of twelve previously unreported cell lines derived from neoplastic and normal epithelium of cancerous prostate revealed different types of HLA-I aberration, ranging from locus and/or allelic downregulation to a total absence of HLA-I expression. The high incidence of HLA-I loss observed in PC, caused by both regulatory and structural defects, is associated with more aggressive disease development and may pose a real threat to patient health by increasing cancer progression and resistance to T-cell-based immunotherapy.
Subject(s)
Histocompatibility Antigens Class I/immunology , Immunotherapy/methods , Prostatic Neoplasms/immunology , beta 2-Microglobulin/immunology , Humans , Male , Neoplasm Recurrence, LocalABSTRACT
Una paciente de 22 años consultó por lesiones vasculares telangiectásicas en hemicara izquierda de 3 años de evolución con características clínicas e histológicas de telangiectasia nevoide unilateral. Se caracteriza por una agrupación de telangiectasias de distribución segmentaria que clásicamente se ha relacionado con estados de hiperestrogenismo. En nuestro caso, y en concordancia con otros autores, la distribución de las lesiones sigue un patrón según las líneas de Blaschko y no metamérico (AU)