Subject(s)
Anaphylaxis , Allergens , Anaphylaxis/diagnosis , Anaphylaxis/etiology , Animals , Fishes , HumansSubject(s)
Humans , Male , Middle Aged , Food Hypersensitivity/etiology , Anaphylaxis/etiology , Fish Products/adverse effectsSubject(s)
Humans , Female , Adult , Anaphylaxis/diagnosis , Anaphylaxis/etiology , Insect Repellents/adverse effects , DEET/adverse effects , Skin TestsSubject(s)
Antineoplastic Agents/therapeutic use , Autoantibodies/blood , Cetuximab/therapeutic use , Desensitization, Immunologic/methods , Immunoglobulin E/blood , alpha-Galactosidase/immunology , Carcinoma, Squamous Cell/drug therapy , Carcinoma, Squamous Cell/immunology , Carcinoma, Squamous Cell/secondary , Drug Administration Schedule , Humans , Lung Neoplasms/drug therapy , Lung Neoplasms/immunology , Lung Neoplasms/secondary , Male , Middle Aged , Pharyngeal Neoplasms/drug therapy , Pharyngeal Neoplasms/immunology , Pharyngeal Neoplasms/pathology , Pyriform Sinus/drug effects , Pyriform Sinus/immunology , Pyriform Sinus/pathology , Skin Tests , alpha-Galactosidase/bloodSubject(s)
Abnormalities, Multiple/diagnosis , Anaphylaxis/diagnosis , Capsicum/immunology , Food Hypersensitivity/diagnosis , Latex Hypersensitivity/diagnosis , Abnormalities, Multiple/immunology , Adult , Allergens/immunology , Anaphylaxis/immunology , Antigens, Plant/immunology , Cross Reactions , Female , Food Hypersensitivity/immunology , Fruit/adverse effects , Humans , Immunoblotting , Immunoglobulin E/immunology , Latex Hypersensitivity/immunology , SyndromeSubject(s)
Anemia, Aplastic/immunology , Antilymphocyte Serum/immunology , Desensitization, Immunologic , Drug Hypersensitivity/therapy , Adolescent , Anemia, Aplastic/complications , Animals , Antilymphocyte Serum/administration & dosage , Antilymphocyte Serum/adverse effects , Drug Hypersensitivity/etiology , Drug Hypersensitivity/immunology , Female , Humans , Rabbits , Skin Tests , Thrombocytopenia/etiologyABSTRACT
Type IV hypersensitivity eye reactions have been described after the administration of the sympathomimetic agent phenylephrine. We report the case of an atopic woman who developed nasal congestion and discharge, dysphagia, and dyspnea 1 hour after the administration of Stopcold pills and Disneumon Pernasal nasal spray for otitis. The same symptoms reappeared after the accidental administration of Rinobanedif ointment in the nasal mucosa. Skin patch tests were performed with a standard True Test panel, preservatives, Disneumon Pernasal, pseudoephedrine, eyedrops (tropicamide, cyclopentolate, and phenylephrine), and other sympathomimetic agents. The patient also underwent oral, ocular, and nasal controlled challenges with the same drugs. Finally, patch tests were performed in 11 controls with phenylephrine and ethylephrine. Our patient had a positive outcome in patch testing with nickel sulphate, fragrance mix, phenylephrine, and ethylephrine. To our knowledge, this is the first report of a type IV reaction to nasally administered phenylephrine with cross-reactivity with ethylephrine detected by patch testing.
Subject(s)
Drug Hypersensitivity/etiology , Hypersensitivity, Delayed/etiology , Phenylephrine/adverse effects , Sympathomimetics/adverse effects , Administration, Intranasal , Cross Reactions , Diagnosis, Differential , Drug Hypersensitivity/diagnosis , Etilefrine/administration & dosage , Etilefrine/adverse effects , Etilefrine/therapeutic use , Female , Humans , Hypersensitivity, Delayed/diagnosis , Middle Aged , Patch Tests , Phenylephrine/administration & dosage , Phenylephrine/therapeutic use , Sympathomimetics/administration & dosage , Sympathomimetics/therapeutic useABSTRACT
BACKGROUND: Nonsynonymous polymorphisms in genes coding for histamine-metabolizing enzymes, diamine oxidase and histamine N-methyltransferase are related to the risk of developing allergic diseases. The role of polymorphisms in the histidine decarboxylase gene remains unexplored. The objective of this study is to identify novel polymorphisms in the human histidine decarboxylase gene and to analyse the clinical association of nonsynonymous polymorphisms with rhinitis. METHODS: We performed a single-strand conformational polymorphism analysis of the histidine decarboxylase gene sequence. The presence of two nonsynonymous polymorphisms Thr31Met (rs17740607) and Glu644Asp (rs2073440) was analysed in 442 unrelated patients with allergic rhinitis, 233 of whom also had asthma, and in 486 healthy subjects. RESULTS: We observed three novel polymorphisms designated as ss50402829, ss50402830 and ss50402831-(rs17740607) with allele frequencies = 0.005, 0.208 and 0.073, respectively. Statistically significant differences were observed for the histidine decarboxylase Glu644Asp (rs2073440) polymorphism, with OR (95% CI) values for homozygous carriers of the Glu644 allele equal to 3.12 (1.75-5.56, P < 0.00005) for all patients, 3.38 (1.54-7.44, P = 0.002) for patients with rhinitis alone, and 2.92 (1.43-5.95), P = 0.003 for patients with rhinitis + asthma, when compared with healthy controls. A significant Glu644 gene-dose effect was observed for overall patients (P = 0.0001), for patients with rhinitis alone (P = 0.005) and for patients with rhinitis + asthma (P = 0.010). CONCLUSIONS: The HDC allele Glu644 in homozygosity increases the risk of developing rhinitis in the studied population. This adds to increasing evidence supporting a prominent role of genetic variations related to histamine homeostasis in the risk to develop allergic diseases.
Subject(s)
Genetic Predisposition to Disease/genetics , Histidine Decarboxylase/genetics , Hypersensitivity/genetics , Rhinitis/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Asthma/complications , Asthma/enzymology , Asthma/genetics , Child , Child, Preschool , Female , Gene Frequency , Haplotypes , Humans , Hypersensitivity/enzymology , Male , Middle Aged , Polymorphism, Single-Stranded Conformational , Rhinitis/complications , Rhinitis/enzymology , Young AdultSubject(s)
Anti-Bacterial Agents/adverse effects , Drug Hypersensitivity/etiology , Furunculosis/drug therapy , Fusidic Acid/adverse effects , Urticaria/etiology , Administration, Oral , Administration, Topical , Adult , Anti-Bacterial Agents/administration & dosage , Drug Hypersensitivity/diagnosis , Female , Fusidic Acid/administration & dosage , Humans , Skin Tests , Urticaria/diagnosisABSTRACT
BACKGROUND: Polymorphisms of enzymes involved in histamine biodisposition may affect clinical symptoms in diseases related to histamine, such as asthma or allergic rhinitis (AR). OBJECTIVE: This study aims to analyse two common polymorphisms in genes coding for histamine-metabolizing enzymes in patients with allergic diseases. METHODS: Five-hundred and sixty-five individuals participated in the study, including 270 unrelated patients with asthma and/or AR recruited from a single centre and 295 healthy volunteers. Participants were analysed for the presence of Thr105Ile and His645Asp amino acid substitutions at histamine N-methyltransferase (HNMT) and diamine oxidase (amiloride binding protein 1) enzymes, respectively, by amplification-restriction procedures. RESULTS: The variant HNMT allele frequencies were slightly higher among patients with asthma [16.0%, 95% confidence interval (CI) 12.0-20.0] and among patients with rhinitis (13.2, 95% CI 10.3-16.1) as compared with healthy subjects (11.5 95% CI 8.9-14.1). The variant ABP1 allele frequencies were similar among patients with asthma (30.8%, 95% CI 25.7-35.9), rhinitis (28.7, 95% CI 24.8-32.6) and healthy subjects (26.8 95% CI 23.2-30.3). Individuals carrying mutated ABP1 alleles presented allergy symptoms with significantly lower IgE levels as compared with individuals without mutated genes, with a significant gene-dose effect (P<0.001). In addition, the percentage of individuals presenting symptoms without eosinophilia was significantly higher among homozygous carriers of ABP1 variant alleles (P<0.020) as compared with the rest of the atopic patients. CONCLUSION: There is a lack of association between the allelic variants studied and the risk of developing allergic asthma and rhinitis. However, patients carrying the His645Asp polymorphism of ABP1 are more prone to developing symptoms with lower IgE levels.
Subject(s)
Alleles , Amine Oxidase (Copper-Containing)/genetics , Asthma/genetics , Histamine N-Methyltransferase/genetics , Polymorphism, Restriction Fragment Length , Rhinitis, Allergic, Perennial/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Amine Oxidase (Copper-Containing)/metabolism , Amino Acid Substitution , Asthma/blood , Child , Child, Preschool , Female , Gene Frequency , Histamine N-Methyltransferase/metabolism , Humans , Immunoglobulin E/blood , Male , Middle Aged , Predictive Value of Tests , Rhinitis, Allergic, Perennial/blood , Risk FactorsABSTRACT
Because seafood consumption is moderate-to-high in Spain, allergic reactions to seafood such as fish, crustacea and mollusc are fairly frequent. The clinical features of these reactions depend on the implicated species and whether the reaction is provoked by ingestion, handling or vapor inhalation. Because different species have common antigenic structures, cross-sensitization is frequent, especially between crustaceans and molluscs. Contamination of fish by nematodes (Anisakis) may produce severe reactions. We report the case of a female patient with no personal or family history of allergy who experienced two episodes of anaphylactic shock: the first occurred immediately after eating oysters and the second after ingestion of white fish. The patient also developed generalized urticaria provoked by crustacean (prawns) and white fish. The results of skin prick tests were negative for fish, shellfish, crustacean and oysters while in vitro tests were positive for oyster, prawns, Anisakis, Ascaris and Echinococcus, although stool samples and gastric endoscopy were negative.
Subject(s)
Anaphylaxis/etiology , Anisakis , Decapoda , Fishes/parasitology , Food Hypersensitivity/etiology , Ostreidae , Seafood/adverse effects , Urticaria/etiology , Allergens , Animals , Anisakis/immunology , Ascaris/immunology , Child , Cross Reactions , Decapoda/immunology , Echinococcus/immunology , Endoscopy, Digestive System , Female , Humans , Middle Aged , Ostreidae/immunology , Skin TestsABSTRACT
Some members of the Anacardiaceae family, such as cashew nut, pistachio nut and mango, have been reported to cause immediate allergic reactions. We report three cases of anaphylaxis to cashew nuts. With the aim of describing the allergens existing in cashew and pistachio nuts, patients were prick tested with cashew and pistachio extracts. Specific IgE against both nuts was studied by CAP and SDS-PAGE/immunoblotting. It was found that skin tests and specific IgE to cashew and pistachio nuts were positive in the three patients. Both nuts showed several protein bands in SDS-PAGE. The strongest IgE-binding bands had similar molecular weights (15, 30 and 60 kDa) in cashew and pistachio nuts. These main bands were found to be sensitive to reducing agents. It was concluded that these three patients suffered immediate reactions to cashew nut due to an IgE-mediated mechanism.
Subject(s)
Allergens/immunology , Food Hypersensitivity/etiology , Nuts/immunology , Trees , Adult , Child , Female , Food Hypersensitivity/blood , Food Hypersensitivity/immunology , Food Hypersensitivity/physiopathology , Humans , Infant , Skin TestsABSTRACT
BACKGROUND: Few cases of allergy to pine nuts have been described. We report a case of anaphylactic reaction to pine nuts. The patient needed to be treated in the emergency room due to a systemic reaction immediately after eating pine nuts. METHODS: The patient was studied by prick tests and prick by prick tests. Specific IgE was measured by CAP and by SDS-PAGE/immunoblotting by a diffusion method. RESULTS: The patient showed positive prick by prick tests to pine nuts (12 mm of maximum wheal diameter). Specific IgE was positive (0.79 kU/l). The patient's serum recognized several proteins by immunoblot. However, a 17-kDa allergen band was detected with high intensity. This protein was found to be sensitive to reducing agents, losing its IgE-binding properties after reduction. CONCLUSIONS: The patient presented an IgE-mediated reaction and detected a 17-kDa protein from pine nuts not previously described.
