ABSTRACT
In this study, we characterized 12 novel polymorphic microsatellite loci for Chinese beard eel (Cirrhimuraena chinensis Kaup) from a tetranucleotide microsatellite-enriched library. Loci screened on a sample of 37 individuals from Xiamen wild stocks revealed 8 to 24 alleles per locus, with a mean of 13.83 over all loci. Observed and expected heterozygosities ranged from 0.270 to 0.944 and 0.439 to 0.942, respectively. These efficient genetic markers thus provide useful tools in the study of the population genetics and phylogeography of Chinese beard eel.
Subject(s)
Eels/genetics , Microsatellite Repeats , Animals , Genetic Loci , Genetic Markers , Heterozygote , Phylogeography , Polymorphism, Genetic , Population/geneticsABSTRACT
Friable embryogenic calli were obtained on a modified N6 medium (NBD medium) from a winter wheat cultivar "Jinghua No. 1" (Triticum aestivum L. cv. Jinghua No. 1) and were transferred to a modified MS liquid medium (MSDL medium) to initiate embryogenic suspension cultures. Protoplasts were isolated from the suspensions and cultured on a modified MS medium (MSDP medium). The somatic embryoids were formed directly from the protoplasts and germinated into entire plants. The development of the somatic embryoids was very similar to that of zygotic embryos of wheat.
