Serosurvey for Canine Influenza in Coyotes (Canis latrans) in Illinois, USA, 2000-23.

Coyotes (Canis latrans) share urban habitats with domestic dogs (Canis lupus familiaris), providing opportunities for pathogen transmission. In Chicago, Illinois, USA, canine influenza virus (CIV) is prevalent in dogs. Serologic investigation for exposure in 101 coyote samples collected 2000-23 did not detect any antibodies against CIV H3N2 and H3N8.

Stress in the city? Coyote hair cortisol varies with intrinsic and extrinsic factors within a heavily urbanized landscape.

Wildlife living in proximity to people are exposed to both natural and anthropogenic factors that may influence cortisol production associated with stress response. While some species, including coyotes (Canis latrans), have become commonplace in developed areas throughout North America, urban individuals still must navigate ever-changing, novel environments and cope with frequent disturbance. Given that coyotes are relatively large predators compared to most other urban wildlife, they face unique pressures such as crossing roadways to use suitable habitat fragments and are at a greater risk of being detected and experiencing negative human interactions. To assess whether urbanization influences hypothalamic-pituitary-adrenal axis activity in free-ranging coyotes, we analyzed cortisol concentration in hair samples from 97 coyotes residing across the urbanization gradient within the Greater Chicago Metropolitan area. As the proportion of developed landcover within coyote home ranges increased, coyotes experienced more stress. Body condition and social status also had strong relationships with stress. Animals in poorer body condition experienced more stress and subordinate coyotes experienced less stress than alphas. We also found some evidence that stress varied seasonally and among different age classes. Understanding how intrinsic and extrinsic factors influence endocrine activity in urban carnivores is vital for predicting how hormone production and related behavioral patterns may change in future populations as more areas become developed.

Infection risk varies within urbanized landscapes: the case of coyotes and heartworm.

BACKGROUND: Urbanization can have profound effects on ecological interactions. For host-pathogen interactions, differences have been detected between urban and non-urban landscapes. However, host-pathogen interactions may also differ within highly heterogeneous, urbanized landscapes. METHODS: We investigated differences in infection risk (i.e., probability of infection) within urbanized landscapes using the coyote (Canis latrans) and mosquito-borne nematode, Dirofilaria immitis (the causative agent for canine heartworm), as a case study. We focused on a coyote population in Chicago for which extensive behavioral and heartworm infection data has been collected between 2001 and 2016. Our objectives were to: (i) determine how onset and duration of the heartworm transmission season varied over the 16-year period and across the urban-suburban gradient; and (ii) investigate how heartworm infection risk in coyotes varied over the years, across the urban-suburban gradient, by coyote characteristics (e.g., age, sex, resident status), and coyote use of the urbanized landscape (e.g., use of urban areas, mosquito habitats). RESULTS: While onset of the heartworm transmission season differed neither by year nor across the urban-suburban gradient, it was longer closer to the core of Chicago. Of the 315 coyotes sampled, 31.1% were infected with D. immitis. Older coyotes and coyotes sampled in later years (i.e., 2012-2016) were more likely to have heartworm. While coyote location in the urban-suburban gradient was not a significant predictor of infection, the proportion of urban land in coyote home ranges was. Importantly, the size and direction of this association varied by age class. For adults and pups, infection risk declined with urbanization, whereas for subadults it increased. Further, models had a higher predictive power when focusing on resident coyotes (and excluding transient coyotes). The proportion of mosquito habitat in coyote home ranges was not a significant predictor of infection. CONCLUSIONS: Our findings suggest that urbanization may affect host exposure to vectors of D. immitis, that risk of infection can vary within urbanized landscapes, and that urbanization-wildlife infection associations may only be detected for animals with certain characteristics (e.g., age class and resident status).

Comparison of Antimicrobial-Resistant Escherichia coli Isolates from Urban Raccoons and Domestic Dogs.

Wildlife can be exposed to antimicrobial-resistant bacteria (ARB) via multiple pathways. Spatial overlap with domestic animals is a prominent exposure pathway. However, most studies of wildlife-domestic animal interfaces have focused on livestock and little is known about the wildlife-companion animal interface. Here, we investigated the prevalence and phylogenetic relatedness of extended-spectrum cephalosporin-resistant (ESC-R) Escherichia coli from raccoons (Procyon lotor) and domestic dogs (Canis lupus familiaris) in the metropolitan area of Chicago, IL, USA. To assess the potential importance of spatial overlap with dogs, we explored whether raccoons sampled at public parks (i.e., parks where people and dogs could enter) differed in prevalence and phylogenetic relatedness of ESC-R E. coli to raccoons sampled at private parks (i.e., parks where people and dogs could not enter). Raccoons had a significantly higher prevalence of ESC-R E. coli (56.9%) than dogs (16.5%). However, the richness of ESC-R E. coli did not vary by host species. Further, core single-nucleotide polymorphism (SNP)-based phylogenetic analyses revealed that isolates did not cluster by host species, and in some cases displayed a high degree of similarity (i.e., differed by less than 20 core SNPs). Spatial overlap analyses revealed that ESC-R E. coli were more likely to be isolated from raccoons at public parks than raccoons at private parks, but only for parks located in suburban areas of Chicago, not urban areas. That said, ESC-R E. coli isolated from raccoons did not genetically cluster by park of origin. Our findings suggest that domestic dogs and urban/suburban raccoons can have a diverse range of ARB, some of which display a high degree of genetic relatedness (i.e., differ by less than 20 core SNPs). Given the differences in prevalence, domestic dogs are unlikely to be an important source of exposure for mesocarnivores in urbanized areas. IMPORTANCE Antimicrobial-resistant bacteria (ARB) have been detected in numerous wildlife species across the globe, which may have important implications for human and animal health. Wildlife can be exposed to ARB via numerous pathways, including via spatial overlap with domestic animals. However, the interface with domestic animals has mostly been explored for livestock and little is known about the interface between wild animals and companion animals. Our work suggests that urban and suburban wildlife can have similar ARB to local domestic dogs, but local dogs are unlikely to be a direct source of exposure for urban-adapted wildlife. This finding is important because it underscores the need to incorporate wildlife into antimicrobial resistance surveillance efforts, and to investigate whether certain urban wildlife species could act as additional epidemiological pathways of exposure for companion animals, and indirectly for humans.

Importance of anthropogenic sources at shaping the antimicrobial resistance profile of a peri-urban mesocarnivore.

Anthropogenically derived antimicrobial-resistant bacteria (ARB) and antimicrobial resistance genes (ARG) have been detected in wildlife. The likelihood of detecting ARB and ARG in wildlife increases with wildlife exposure to anthropogenic sources of antimicrobial resistance (AMR). Whether anthropogenic sources also increase the risk for AMR to spread in bacteria of wildlife is not well understood. The spread of AMR in bacteria of wildlife can be estimated by examining the richness of ARB and ARG, and the prevalence of ARB that have mobilizable ARG (i.e., ARG that can be transferred across bacteria via plasmids). Here, we investigated whether raccoons (Procyon lotor), with different exposures to anthropogenic sources, differed in prevalence and richness of extended-spectrum cephalosporin-resistant (ESC-R) Escherichia coli, richness of ARG present in ESC-R E. coli, and prevalence of ESC-R E. coli with plasmid-associated ARG. Sampling took place over the course of 10 months at seven sites in Chicago, USA. ESC-R E. coli were isolated from over half of the 211 raccoons sampled and were more likely to be isolated from urban than suburban raccoons. When examining the whole-genome sequences of ESC-R E. coli, 56 sequence types were identified, most of which were associated with the ARG blaCMY and blaCTX-M. A greater richness of ESC-R E. coli sequence types was found at sites with a wastewater treatment plant (WWTP) than without, but no difference was detected based on urban context. ARG richness in ESC-R E. coli did not significantly vary by urban context nor with presence of a WWTP. Importantly, ESC-R E. coli carrying plasmid-associated blaCTX-M and blaCMY ARG were more likely to be isolated from raccoons sampled at sites with a WWTP than without. Our findings indicate that anthropogenic sources may shape the AMR profile of wildlife, reinforcing the need to prevent dissemination of AMR into the environment.

Seasonal and daily shifts in behavior and resource selection: how a carnivore navigates costly landscapes.

The dynamic environmental conditions in highly seasonal systems likely have a strong influence on how species use the landscape. Animals must balance seasonal and daily changes to landscape risk with the underlying resources provided by that landscape. One way to balance the seasonal and daily changes in the costs and benefits of a landscape is through behaviorally-explicit resource selection and temporal partitioning. Here, we test whether resource selection of coyotes (Canis latrans) in Cape Breton Highlands National Park, Nova Scotia, Canada is behaviorally-explicit and responsive to the daily and seasonal variation to presumed costs and benefits of moving on the landscape. We used GPS data and local convex hulls to estimate space use and Hidden Markov Models to estimate three types of movement behavior: encamped, foraging, and traveling. We then used integrated step-selection analysis to investigate behaviorally explicit resource selection across times of day (diurnal, crepuscular, and nocturnal) and season (snow-free and snow). We found that throughout the day and seasonally coyotes shifted foraging behavior and altered behavior and resource choices to avoid moving across what we could be a challenging landscape. These changes in behavior suggest that coyotes have a complex response to land cover, terrain, and linear corridors that are not only scale dependent but also vary by behavior, diel period, and season. By examining the resource selection across three axes (behavior, time of day, and season), we have a more nuanced understanding of how a predator balances the cost and benefits of a stochastic environment.

Characterization of antimicrobial resistance genes in Enterobacteriaceae carried by suburban mesocarnivores and locally owned and stray dogs.

The role of wildlife in the dissemination of antimicrobial-resistant bacteria and antimicrobial resistance genes (ARGs) in the environment is of increasing concern. We investigated the occurrence, richness and transmissibility potential of ARGs detected in the faeces of three mesocarnivore species: the coyote (Canis latrans), raccoon (Procyon lotor) and Virginia opossum (Didelphis virginiana), and of stray and owned dogs in suburban Chicago, IL, USA. Rectal swabs were collected from live-captured coyotes (n = 32), raccoons (n = 31) and Virginia opossums (n = 22). Fresh faecal samples were collected from locally owned (n = 13) and stray dogs (n = 18) and from the live-captured mesocarnivores, when available. Faecal samples and rectal swabs were enriched to select for Enterobacteriaceae and pooled by mesocarnivore species and dog type (owned or stray). Pooled enriched samples were then analysed for the presence of ARGs using shotgun sequencing. The three mesocarnivore and stray dog samples had twice as many unique ARGs compared to the owned dog sample, which was partly driven by a greater richness of beta-lactamase genes (genes conferring resistance to penicillins and cephalosporins). Raccoon and stray dog samples had the most ARGs in common, suggesting possible exposure to similar environmental sources of ARGs. In addition to identifying clinically relevant ARGs (e.g. blaCMY and qnrB), some ARGs were linked to the class 1 integrase gene, intI1, which may indicate anthropogenic origin. Findings from this pilot investigation suggest that the microbial communities of suburban mesocarnivores and stray dogs can host ARGs that can confer resistance to several antimicrobials used in human and veterinary medicine.

Behavioral responses by an apex predator to urbanization.

Wildlife can respond to urbanization positively (synanthropic) or negatively (misanthropic), and for some species, this is a nonlinear process, whereby low levels of urbanization elicit a positive response, but this response becomes negative at high levels of urbanization. We applied concepts from foraging theory to predict positive and negative behavioral responses of coyotes (Canis latrans) along an urbanization gradient in the Chicago metropolitan area, USA. We estimated home range size and complexity, and metrics of 3 movement behaviors (encamped, foraging, and traveling) using Hidden Markov movement models. We found coyotes exhibited negative behavioral responses to highly urbanized landscapes: coyotes viewed the landscape as lower quality, riskier, and more fragmented (home range size and complexity, and time spent encamped increased). Conversely, we found evidence of both positive and negative responses to suburban landscapes: coyotes not only viewed the landscape as higher quality than natural fragments and equally risky, but also viewed it as fragmented (home range size decreased, time spent encamped did not change, and home range complexity increased). Although the spatial and behavioral responses of coyotes to urbanization became increasingly negative as urbanization increased, coyotes were still able to occupy highly urbanized landscapes. Our study demonstrates how wildlife behavioral responses can be dependent on the degree of urbanization and represents one of the first descriptions of apex predator space use and movement in a highly urbanized landscape.

THE STRUCTURE AND SEASONALITY OF BAYLISASCARIS PROCYONIS POPULATIONS IN RACCOONS (PROCYON LOTOR).

Baylisascaris procyonis , the raccoon ( Procyon lotor ) ascarid, is a common roundworm parasite of raccoons that is also a well-recognized zoonotic pathogen, and a cause for conservation concern. The transmission dynamics of B. procyonis differ with host population attributes, season, and landscape. We examined how the parasite's population attributes change with season, parasite population structure, and host demographics. We examined 1,050 raccoon gastrointestinal tracts collected from 1996 to 2012. Of the 1,050 raccoons necropsied, 382 (36%) were infected with at least one B. procyonis (x¯=15.8 [95% confidence interval=13.39-18.26]; median=7; range 1-199 worms/host), and populations were overdispersed. There was a seasonal change in prevalence with a peak in October/November. Worm burdens decreased approximately 28% per month from January to June and increased approximately 31% per month from June to December. The sex structure of B. procyonis populations was female-biased (56% female). Host demographics did not impact parasite population attributes. This study provides evidence that B. procyonis populations exhibit a yearly cycle of loss and recruitment that may impact the transmission dynamics of the parasite.

Raccoon contact networks predict seasonal susceptibility to rabies outbreaks and limitations of vaccination.

Infectious disease transmission often depends on the contact structure of host populations. Although it is often challenging to capture the contact structure in wild animals, new technology has enabled biologists to obtain detailed temporal information on wildlife social contacts. In this study, we investigated the effects of raccoon contact patterns on rabies spread using network modelling. Raccoons (Procyon lotor) play an important role in the maintenance of rabies in the United States. It is crucial to understand how contact patterns influence the spread of rabies in raccoon populations in order to design effective control measures and to prevent transmission to human populations and other animals. We constructed a dynamic system of contact networks based on empirical data from proximity logging collars on a wild suburban raccoon population and then simulated rabies spread across these networks. Our contact networks incorporated the number and duration of raccoon interactions. We included differences in contacts according to sex and season, and both short-term acquaintances and long-term associations. Raccoons may display different behaviours when infectious, including aggression (furious behaviour) and impaired mobility (dumb behaviour); the network model was used to assess the impact of potential behavioural changes in rabid raccoons. We also tested the effectiveness of different vaccination coverage levels. Our results demonstrate that when rabies enters a suburban raccoon population, the likelihood of a disease outbreak affecting the majority of the population is high. Both the magnitude of rabies outbreaks and the speed of rabies spread depend strongly on the time of year that rabies is introduced into the population. When there is a combination of dumb and furious behaviours in the rabid raccoon population, there are similar outbreak sizes and speed of spread to when there are no behavioural changes due to rabies infection. By incorporating detailed data describing the variation in raccoon contact rates into a network modelling approach, we were able to show that suburban raccoon populations are highly susceptible to rabies outbreaks, that the risk of large outbreaks varies seasonally and that current vaccination target levels may be inadequate to prevent the spread of rabies within these populations. Our findings provide new insights into rabies dynamics in raccoon populations and have important implications for disease control.

Individual variation in anthropogenic resource use in an urban carnivore.

With increasing urbanization, some animals are adapting to human-dominated systems, offering unique opportunities to study individual adaptation to novel environments. One hypothesis for why some wildlife succeed in urban areas is that they are subsidized with anthropogenic food. Here, we combine individual-level movement patterns with diet composition based on stable isotope analysis to assess the degree to which a rapidly growing population of coyotes (Canis latrans) in Chicago consumes anthropogenic resources. We used telemetry to classify coyotes into three groups based on social class and home range composition: (1) residents with home ranges in urban nature preserves; (2) residents with home ranges that had a high proportion of urban land; and (3) transients that had relatively large home ranges and variable use of urban land. We found that natural and anthropogenic resources in this system can be reliably partitioned with carbon isotopes. Mixing models revealed that resident coyotes associated with most urban nature preserves consumed trace to minimal amounts of anthropogenic resources, while coyotes that live in the urban matrix consume moderate (30-50%) to high (>50%) proportions of anthropogenic resources. Lastly, we found evidence of prey switching between natural and anthropogenic resources and a high degree of inter-individual variation in diet among coyotes. In contrast to the expectation that urban adaptation may dampen ecological variation, our results suggest individuality in movement and diet exemplifies the successful establishment of coyotes in urban Chicago. Our study also suggests that direct anthropogenic food subsidization is not a prerequisite for successful adaptation to urban environments.

Roosting and foraging social structure of the endangered Indiana bat (Myotis sodalis).

Social dynamics are an important but poorly understood aspect of bat ecology. Herein we use a combination of graph theoretic and spatial approaches to describe the roost and social network characteristics and foraging associations of an Indiana bat (Myotis sodalis) maternity colony in an agricultural landscape in Ohio, USA. We tracked 46 bats to 50 roosts (423 total relocations) and collected 2,306 foraging locations for 40 bats during the summers of 2009 and 2010. We found the colony roosting network was highly centralized in both years and that roost and social networks differed significantly from random networks. Roost and social network structure also differed substantially between years. Social network structure appeared to be unrelated to segregation of roosts between age classes. For bats whose individual foraging ranges were calculated, many shared foraging space with at least one other bat. Compared across all possible bat dyads, 47% and 43% of the dyads showed more than expected overlap of foraging areas in 2009 and 2010 respectively. Colony roosting area differed between years, but the roosting area centroid shifted only 332 m. In contrast, whole colony foraging area use was similar between years. Random roost removal simulations suggest that Indiana bat colonies may be robust to loss of a limited number of roosts but may respond differently from year to year. Our study emphasizes the utility of graphic theoretic and spatial approaches for examining the sociality and roosting behavior of bats. Detailed knowledge of the relationships between social and spatial aspects of bat ecology could greatly increase conservation effectiveness by allowing more structured approaches to roost and habitat retention for tree-roosting, socially-aggregating bat species.

Patterns of latrine use by raccoons (Procyon lotor) and implication for Baylisascaris procyonis transmission.

Mammals often use latrine sites for defecation, yet little is known about patterns of latrine use in many common species such as raccoons (Procyon lotor). Because raccoon latrines are important foci for the transmission of raccoon roundworm (Baylisascaris procyonis), documenting metrics of raccoon latrine use may have public health implications. Although some studies have provided evidence that multiple raccoons visit single latrine sites, exact latrine visitation patterns of raccoons have never been documented. We monitored raccoon latrine usage using proximity-logging collars placed at 15 latrine sites. We found that latrine sites were visited by multiple raccoons (range 1-7), and raccoons visited as many as six latrines during a 2-wk period. No sex differences were found in the number of latrines visited or time spent during visits. We posit that the use of multiple latrine sites by raccoons may lead to the pattern that rates of B. procyonis infection at latrines are greater than infection rates found in individual raccoon fecal samples. This in turn could lead to greater transmission of B. procyonis to paratenic hosts. Our results support the conclusion that raccoon latrines can be major foci for the infection and spread of B. procyonis.

Disease control through fertility control: Secondary benefits of animal birth control in Indian street dogs.

We sought to (1) survey sexually intact street dogs for a wide range of diseases in three cities in Rajasthan, India and (2) evaluate links between the health of non-treated dogs and both the presence and duration of animal birth control (ABC) programs. ABC regimes sterilize and vaccinate stray dogs in an attempt to control their population and the spread of rabies. They are commonly suggested to improve the health of those dogs they serve, but here we provide evidence that these benefits also extend to untreated dogs in the community. Viral and bacterial disease seroprevalences were assessed in 240 sexually intact street dogs from Jaipur, Jodhpur, and Sawai Madhopur cities in October and September 2011. Those individuals and 50 additional dogs were assessed for the presence of ticks, fleas, fight wounds, and given body condition scores. Dogs in cities with an ABC program had with significantly (p<0.05) higher overall body condition scores, lower prevalence of open wounds likely caused by fighting, flea infestations, infectious canine hepatitis, Ehrlichia canis, Leptospira interrogans serovars, and canine distemper virus antibodies. However, those same dogs in cities with ABC programs had significantly higher prevalence of Brown Dog Tick (Rhipicephalus sanguineus) infestations. Canine parvovirus and Brucella canis prevalences were not significantly different between cities. This study is the first to demonstrate the health benefits of ABC on non-vaccinated diseases and non-treated individuals.

Raccoon social networks and the potential for disease transmission.

Raccoons are an important vector of rabies and other pathogens. The degree to which these pathogens can spread through a raccoon population should be closely linked to association rates between individual raccoons. Most studies of raccoon sociality have found patterns consistent with low levels of social connectivity within populations, thus the likelihood of direct pathogen transmission between raccoons is theoretically low. We used proximity detecting collars and social network metrics to calculate the degree of social connectivity in an urban raccoon population for purposes of estimating potential pathogen spread. In contrast to previous assumptions, raccoon social association networks were highly connected, and all individuals were connected to one large social network during 15 out of 18 months of study. However, these metrics may overestimate the potential for a pathogen to spread through a population, as many of the social connections were based on relatively short contact periods. To more closely reflect varying probabilities of pathogen spread, we censored the raccoon social networks based on the total amount of time spent in close proximity between two individuals per month. As this time criteria for censoring the social networks increased from one to thirty minutes, corresponding measures of network connectivity declined. These findings demonstrate that raccoon populations are much more tightly connected than would have been predicted based on previous studies, but also point out that additional research is needed to calculate more precise transmission probabilities by infected individuals, and determine how disease infection changes normal social behaviors.

Population ecology of free-roaming cats and interference competition by coyotes in urban parks.

Free-roaming cats are a common element of urban landscapes worldwide, often causing controversy regarding their impacts on ecological systems and public health. We monitored cats within natural habitat fragments in the Chicago metropolitan area to characterize population demographics, disease prevalence, movement patterns and habitat selection, in addition to assessing the possible influence of coyotes on cats. The population was dominated by adults of both sexes, and 24% of adults were in reproductive condition. Annual survival rate was relatively high (S=0.70, SE=0.10), with vehicles and predation the primary causes of death. Size of annual home range varied by sex, but not reproductive status or body weight. We observed partitioning of the landscape by cats and coyotes, with little interspecific overlap between core areas of activity. Coyotes selected for natural habitats whereas cats selected for developed areas such as residences. Free-roaming cats were in better condition than we predicted, but their use of natural habitat fragments, and presumably their ecological impact, appeared to be limited by coyotes through intraguild competition.

Pathogen dynamics and morbidity of striped skunks in the absence of rabies.

Parasites have the potential to influence the population dynamics of mammalian hosts, either as a single devastating pathogen or as a community effect. Striped skunks (Mephitis mephitis) are typically host to rabies, which often regulates population numbers. We assessed micro- and macroparasite dynamics in striped skunk populations in the absence of rabies, to determine if a single pathogen, or community, was responsible for a majority of skunk deaths. We monitored mortality due to pathogens, and prevalence of pathogens via serology and necropsy, in two populations of striped skunks in northern Illinois during 1998-2004. Transmissible pathogens requiring direct transmission (i.e., canine distemper virus, canine parvovirus) exhibited high annual variability in prevalence. In contrast, those pathogens employing a more indirect, environmental route of transmission (i.e., Leptospira interrogans and Toxoplasma gondii) appeared to exhibit relatively less annual variability in prevalence. Skunks were diagnosed with infections from an average of 4.08 (SD=2.52, n=32) species of endoparasites, with a range of 1-11. Macroparasite prevalence and intensity did not vary among seasons, or sex or age of host. Severe infections occurred with multiple parasite species, and patterns of aggregation suggested some parasite species, or more likely the parasite community, act as a limiting mechanism in skunk populations.

The relationship between Baylisascaris procyonis prevalence and raccoon population structure.

Parasite transmission is a dynamic process that can be affected by factors including host and parasite population dynamics. Raccoons ( Procyon lotor ) are the definitive host of Baylisascaris procyonis , an intestinal roundworm. Transmission of this parasite has been linked to raccoon behavior and human land-use patterns; however, we do not know the importance of host population structure. Therefore, the objective of this study was to determine the relationship between raccoon population attributes and prevalence of B. procyonis. We necropsied 307 trapped or road-killed raccoons collected during 2000-2006 from the Chicago area. In addition, we examined, via fecal samples (n  =  433), the patterns of B. procyonis prevalence as they relate to population dynamics among 3 subpopulations within the larger study. Baylisascaris procyonis was seen in 39% of 307 necropsied raccoons. There were differences in prevalence as a function of host age and sex. Baylisascaris procyonis was observed in 18% of 433 fecal samples obtained from live-trapped raccoons, and there were differences according to age, but not by sex. We found that the host populations consistently differed in density across study areas, but were similar regarding sex and age structure. Differences in host density were associated with differences in prevalence, suggesting that possible differences between populations, as well as ecological differences in sites and raccoon behavior, may have influenced parasite prevalence.

Land-use effects on prevalence of raccoon roundworm (Baylisascaris procyonis).

The raccoon (Procyon lotor) is the definitive host of Baylisascaris procyonis, a large intestinal roundworm that is zoonotic and can result in fatal or severe central nervous system disease in young children. Prevalence of infection among raccoon populations often is high, and in the midwestern United States, B. procyonis has been reported in 68-82% of raccoons. Raccoon populations have increased in response to changes in human land use, and often reach higher densities in urban and suburban landscapes than rural landscapes. However, shifts in foraging behavior among urban raccoons could impact the transmission of B. procyonis if small vertebrate intermediate hosts are not a significant part of the raccoon diet. The objective of this study was to compare prevalence of B. procyonis infection between urban and rural raccoon populations on a regional scale. Necropsy was done on 204 raccoons collected from September through February during 2000-2005 from seven states across the Midwest (regional sample). Baylisascaris procyonis was found in 54% of examined raccoons. Prevalence differed between land-use types (chi2=11.56, df=1, P=0.0007), and was higher among animals collected from rural locations (65%) than those collected in urban locations (41%). Intensity of infection also differed (F=5.52, df=1, P=0.02), with rural raccoons having greater worm burdens (x=29.63+/-36.42) than urban raccoons (x=13.85+/-18.47). Despite high densities of raccoons in urban landscapes, fewer urban raccoons were infected with B. procyonis, suggesting decreased dependence on intermediate hosts as a food source. This possible explanation was supported by a similar trend in prevalence among subsamples of raccoons collected from three Chicago-area populations (local samples) with differing levels of urbanization, population densities, and foraging behavior that had been intensively monitored during 1995-2002. Decreased transmission of B. procyonis in urban landscapes may be due to decreased predation of intermediate hosts, and contact of juvenile raccoons with B. procyonis eggs may be an important factor in maintaining infections within such populations.