ABSTRACT
INTRODUCTION: A farm belonging to a Swiss sow pool system reported increased cases of necrosis on the base of the tail or ears in their piglets. Therefore, herd examination was performed in February 2021, and it was found that about half of all examined litters included piglets with necrosis of different locations, and that the sows of these piglets were rather thin. Upon instruction, the farmer then documented the body condition score (BCS) and weight before farrowing and after weaning, and the number of liveborn piglets affected by necrosis of the tail or ear of the next four farrowing batches. In total, data of 97 sows with 1214 liveborn piglets were evaluated. Sows were retrospectively allocated into two groups: Those with piglets with ear and/or tail necrosis (NE), and those without (WN). Of the 97 litters, 40 included piglets with necrosis, with 28 of them having piglets only with tail necrosis, 8 only with ear necrosis, and 4 litters included piglets with both types of necrosis. The group NE lost significantly more weight and BCS points over the suckling period than the group WN, with a tendency of having a lower BCS after weaning (2,0 vs. 2,25/5,0). Blood samples of five sows were analyzed and tested positive for the Fusarium mycotoxin deoxynivalenol (DON). It could be possible that the sows previously consumed DON contaminated feed, which was then stored in their fat tissue, and released again into the blood stream during increased weight loss. Since DON can be transferred from the sow to her piglets during gestation or lactation, this release might have affected the piglets, leading to tail or ear necrosis. However, causative studies are needed to confirm this hypothesis.
INTRODUCTION: Une exploitation appartenant à un système suisse de pool de truies a signalé une augmentation des cas de nécrose à la base de la queue ou des oreilles chez ses porcelets. Par conséquent, un examen de l'effectif a été effectué en février 2021 et il a été constaté qu'environ la moitié de toutes les portées examinées comprenaient des porcelets présentant des nécroses à différents endroits et que les mères de ces porcelets étaient plutôt maigres. Sur instruction, l'éleveur a ensuite documenté la note d'état corporel (BCS) et le poids avant la mise bas et après le sevrage, ainsi que le nombre de porcelets nés vivants affectés par une nécrose de la queue ou de l'oreille des quatre lots de mise bas suivants. Au total, les données de 97 truies avec 1214 porcelets nés vivants ont été évaluées. Les truies ont été réparties rétrospectivement en deux groupes : Celles dont les porcelets présentaient une nécrose de l'oreille et/ou de la queue (NE), et celles qui n'en présentaient pas (WN). Sur les 97 portées, 40 comprenaient des porcelets atteints de nécrose, dont 28 uniquement avec une nécrose de la queue, 8 uniquement avec une nécrose de l'oreille et 4 avec les deux types de nécrose. Le groupe NE a perdu beaucoup plus de poids et de points BCS pendant la période d'allaitement que le groupe WN, avec une tendance à avoir un BCS plus faible après le sevrage (2,0 vs. 2,25/5,0). Les échantillons de sang de cinq truies ont été analysés et se sont révélés positifs pour la mycotoxine de Fusarium, le déoxynivalénol (DON). Il est possible que les truies aient consommé des aliments contaminés par le DON qui a ensuite été stocké dans leur tissu adipeux puis libéré dans le sang lors de la perte de poids. Comme le DON peut être transféré de la truie à ses porcelets pendant la gestation ou la lactation, cette libération pourrait avoir affecté les porcelets, entraînant une nécrose de la queue ou des oreilles. Cependant, des études causales sont nécessaires pour confirmer cette hypothèse.
Subject(s)
Swine Diseases , Tail , Swine , Animals , Female , Retrospective Studies , Necrosis/veterinary , Weaning , Weight LossABSTRACT
INTRODUCTION: Tail biting and lesions are common problems in modern pig production. In 2008 tail docking to prevent tail biting was banned in Switzerland. Since then pigs have been raised with intact tails. This study aimed to assess the current prevalence of tail lesions at Swiss abattoirs and comparing abattoir data with farm-specific data regarding potential risk factors for tail lesions. Data collection was performed in repetitive cycles of two weeks at four abattoirs during all consecutive seasons of one year. Gender, tail length and the tail tip condition were evaluated among other parameters. During 32 weeks in total, 195 704 pigs from 6112 batches from 2510 herds were evaluated. Overall, 63,2 % of the animals included in the analysis were slaughtered with a complete tail (lowest tail length score [TLS]), whereas 36,8 % showed a partial or total loss of the tail. The condition of the tail tip (tail tip condition score [TTCS]) was judged as being intact in 63,0 %, as a healed lesion in 23,7 %, an acute lesion in 1,3 % and a chronic lesion in 12,0 % of all cases. Male animals had significantly higher values for TLS and TTCS than female animals (P ≤ 0,05). TLS values were significantly higher in winter than in spring and summer (P < 0,001). TTCS values were significantly higher in fall than in spring and summer. TLS and TTCS values differed significantly (P < 0,001) between the four abattoirs. Only few significant correlations were found between values of TLS and TTCS and farm-specific data. Recording tail lesions at abattoirs is an accurate method to investigate the prevalence of tail lesions in fattening pigs. However, to monitor animal welfare on herd level, this method is very labor intensive. Moreover, data on tail lesions collected at the abattoir cannot replace veterinary on-farm examination for risk factor identification.
INTRODUCTION: Les morsures et les lésions de la queue sont des problèmes courants dans la production porcine moderne. En 2008, la Suisse a interdit la caudectomie pour prévenir les morsures de la queue. Depuis lors, les porcs sont engraissés avec des queues intactes. Cette étude visait à évaluer la prévalence actuelle des lésions de la queue dans les abattoirs suisses et à comparer les données de l'abattoir avec les données spécifiques à l'exploitation concernant les facteurs de risque potentiels pour des lésions de la queue. La collecte des données a été effectuée par cycles répétitifs de deux semaines dans quatre abattoirs pendant toutes les saisons d'une année. Le sexe, la longueur de la queue et l'état de l'extrémité de la queue ont été évalués parmi d'autres paramètres. Pendant 32 semaines au total, 195 704 porcs provenant de 6 112 lots de 2 510 troupeaux ont été évalués. Dans l'ensemble, 63,2 % des animaux inclus dans l'analyse ont été abattus avec une queue complète (Tail Length Score [TLS] la plus basse), tandis que 36,8 %présentaient une perte partielle ou totale de la queue. L'état de l'extrémité de la queue (Tail Tip Condition Score [TTCS]) a été jugé intact dans 63,0 %des cas, avec une lésion cicatrisée dans 23,7 %des cas, avec une lésion aiguë dans 1,3 %des cas et avec une lésion chronique dans 12,0 %des cas. Les animaux mâles présentaient des valeurs de TLS et de TTCS significativement plus élevées que les animaux femelles (P ≤ 0,05). Les valeurs de TLS étaient significativement plus élevées en hiver qu'au printemps et en été (P < 0,001). Les valeurs de TTCS étaient significativement plus élevées en automne qu'au printemps et en été. Les valeurs TLS et TTCS différaient significativement (P < 0,001) entre les quatre abattoirs. Seules quelques corrélations significatives ont été trouvées entre les valeurs de TLS et TTCS et les données spécifiques à l'exploitation. L'enregistrement des lésions de la queue dans les abattoirs est une méthode précise pour étudier la prévalence de ces lésions chez les porcs d'engraissement. Cependant, pour contrôler le bien-être animal au niveau du troupeau, cette méthode demande beaucoup de travail. En outre, les données sur les lésions de la queue collectées à l'abattoir ne peuvent pas remplacer les examens vétérinaires sur l'exploitation pour l'identification des facteurs de risque.
Subject(s)
Bites and Stings , Tail , Abattoirs , Animal Welfare , Animals , Bites and Stings/veterinary , Female , Male , Prevalence , Swine , Switzerland/epidemiology , Tail/injuriesABSTRACT
A full-length and a C-terminally truncated form of the calcium channel alpha(1S) subunit can be isolated from skeletal muscle. Here we studied whether full-length alpha(1S) is functionally incorporated into the skeletal muscle excitation-contraction coupling apparatus. A fusion protein of alpha(1S) with the green fluorescent protein attached to its C-terminus (alpha(1S)-GFP) or alpha(1S) and GFP separately (alpha(1S)+GFP) were expressed in dysgenic myotubes, which lack endogenous alpha(1S). Full-length alpha(1S)-GFP was targeted into triad junctions and restored calcium currents and excitation-contraction coupling. GFP remained colocalized with alpha(1S), indicating that intact alpha(1S)-GFP was inserted into triads and that the C-terminus remained associated with the excitation-contraction coupling apparatus.
Subject(s)
Calcium Channels, L-Type/genetics , Gene Expression , Muscle, Skeletal/metabolism , Transfection , Calcium Channels, L-Type/deficiency , Calcium Channels, L-Type/physiology , Cell Line , Cell Membrane/metabolism , Electric Conductivity , Electric Stimulation , Green Fluorescent Proteins , Luminescent Proteins/genetics , Microtubules/metabolism , Muscle Contraction , Recombinant Fusion Proteins , Sarcoplasmic Reticulum/metabolismABSTRACT
1. The beta subunits of voltage-sensitive calcium channels facilitate the incorporation of channels into the plasma membrane and modulate calcium currents. In order to determine whether these two effects of the beta subunit are interdependent or independent of each other we studied plasma membrane incorporation of the channel subunits with green fluorescent protein and immunofluorescence labelling, and current modulation with whole-cell and single-channel patch-clamp recordings in transiently transfected human embryonic kidney tsA201 cells. 2. Coexpression of rabbit cardiac muscle alpha1C with rabbit skeletal muscle beta1a, rabbit heart/brain beta2a or rat brain beta3 subunits resulted in the colocalization of alpha1C with beta and in a marked translocation of the channel complexes into the plasma membrane. In parallel, the whole-cell current density and single-channel open probability were increased. Furthermore, the beta2a isoform specifically altered the voltage dependence of current activation and the inactivation kinetics. 3. A single amino acid substitution in the beta subunit interaction domain of alpha1C (alpha1CY467S) disrupted the colocalization and plasma membrane targeting of both subunits without affecting the beta subunit-induced modulation of whole-cell currents and single-channel properties. 4. These results show that the modulation of calcium currents by beta subunits can be explained by beta subunit-induced changes of single-channel properties, but the formation of stable alpha1C-beta complexes and their increased incorporation into the plasma membrane appear not to be necessary for functional modulation.
Subject(s)
Calcium Channels/physiology , Animals , Calcium Channels/genetics , Calcium Channels/metabolism , Cell Line , Cell Membrane/metabolism , Cell Membrane/physiology , Electric Conductivity , Homeostasis/physiology , Humans , Kinetics , Mutation/physiology , Probability , Protein Isoforms/metabolism , Protein Isoforms/physiology , Rabbits , Rats , Tissue Distribution/physiologyABSTRACT
The skeletal muscle L-type Ca2+ channel is a complex of five subunits that is specifically localized in the triad. Its primary function is the rapid activation of Ca2+ release from cytoplasmic stores in a process called excitation-contraction coupling. To study the role of alpha1S-beta1a interactions in the incorporation of the functional channel complex into the triad, alpha1S and beta1a [or a beta1a-green fluorescent protein (GFP) fusion protein] were expressed alone and in combination in myotubes of the dysgenic cell line GLT. betaGFP expressed in dysgenic myotubes that lack the skeletal muscle alpha1S subunit was diffusely distributed in the cytoplasm. On coexpression with the alpha1S subunit betaGFP distribution became clustered and colocalized with alpha1S immunofluorescence. Based on the colocalization of betaGFP and alpha1S with the ryanodine receptor the clusters were identified as T-tubule/sarcoplasmic reticulum junctions. Expression of alpha1S with and without beta1a restored Ca2+ currents and depolarization-induced Ca2+ release. The translocation of betaGFP from the cytoplasm into the junctions failed when betaGFP was coexpressed with alpha1S mutants in which the beta interaction domain had been altered (alpha1S-Y366S) or deleted (alpha1S-Delta351-380). Although alpha1S-Y366S did not associate with betaGFP it was incorporated into the junctions, and it restored Ca2+ currents and depolarization-induced Ca2+ release. Thus, beta1a requires the association with the beta interaction domain in the I-II cytoplasmic loop of alpha1S for its own incorporation into triad junctions, but stable alpha1S-beta1a association is not necessary for the targeting of alpha1S into the triads or for its normal function in Ca2+ conductance and excitation-contraction coupling.
Subject(s)
Calcium Channels/chemistry , Calcium/physiology , Muscle, Skeletal/ultrastructure , Animals , Cell Membrane/metabolism , Cells, Cultured , Electrophysiology , Fluorescent Antibody Technique, Indirect , Macromolecular Substances , Mice , Muscle Contraction , Muscle, Skeletal/chemistry , Myotonic Dystrophy , Sarcoplasmic Reticulum/metabolism , TransfectionABSTRACT
To study the interactions of the alpha1S subunit of the skeletal muscle L-type Ca2+ channel with the skeletal beta1a and the cardiac beta2a, these subunits were expressed alone or in combination in tsA201 cells. Immunofluorescence- and green fluorescent protein-labeling showed that, when expressed alone, beta1a was diffusely distributed throughout the cytoplasm, beta2a was localized in the plasma membrane, and alpha1S was concentrated in a tubular/reticular membrane system, presumably the endoplasmic reticulum (ER). Upon coexpression with alpha1S, beta1a became colocalized with alpha1S in the ER. Upon coexpression with beta2a, alpha1S redistributed to the plasma membrane, where it aggregated in large clusters. Coexpression of alpha1S with beta1a but not with beta2a increased the frequency at which cells expressed L-type currents. A point mutation (alpha1S-Y366S) or deletion (alpha1S-Delta351-380) in the beta interaction domain of alpha1S blocked both translocation of beta1a to the ER and beta2a-induced translocation of the alpha1S mutants to the plasma membrane. However, the point mutation did not interfere with beta1a-induced current stimulation. Thus, beta1a and beta2a are differentially distributed in tsA201 cells and upon coexpression with alpha1S, form alpha1S. beta complexes in different cellular compartments. Complex formation but not current stimulation requires the intact beta interaction domain in the I-II cytoplasmic loop of alpha1S.
Subject(s)
Calcium Channels/chemistry , Calcium Channels/physiology , Muscle, Skeletal/metabolism , Myocardium/metabolism , Animals , Calcium Channels/biosynthesis , Calcium Channels, L-Type , Cell Line , DNA Primers , Green Fluorescent Proteins , Humans , Kidney , Luminescent Proteins/biosynthesis , Macromolecular Substances , Membrane Potentials , Mutagenesis, Site-Directed , Point Mutation , Polymerase Chain Reaction , Protein Multimerization , Rabbits , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/chemistry , Sequence Deletion , Subcellular Fractions/metabolism , TransfectionABSTRACT
The flash-induced Ca(2+)- and Na(+)-influx and Na+/Ca(2+)-exchange activity in blowfly Calliphora photoreceptors were investigated. The change in membrane potential, induced by a bright flash, was intracellularly measured in vivo. Based on a biophysical photoreceptor model, the Na(+)- and Ca(2+)-currents and concentration changes were determined from the first transient depolarization phase of the photoreceptor response. The activity of Na+/Ca(2+)-exchange was determined from the after depolarization phase. It appeared that the Na(+)-influx by Na+/Ca(2+)-exchange is about twice that through light-activated channels, suggesting a substantial contribution of Na+/Ca(2+)-exchange to Na(+)-regulation.