1.
Carbohydr Res
; 481: 67-71, 2019 Jul 15.
Article
in English
| MEDLINE
| ID: mdl-31252337
ABSTRACT
A series of 3-carbamoyl- and 2,3-dicarbamoyl-mannose derivatives were synthesized, conjugated to a fluorescent dye (Cy5GE, AF 647 or NBD) and their cellular uptake in A549 and THP-1â¯cell lines was studied by FACS. In contrast to earlier studies on carbamoyl mannosides, the observed uptake was not related to carbamoyl group on the mannose residue but rather to the cyanine dye attached, a trend previously observed for Cy5-fructose conjugates. The NBD-conjugates however, showed a temperature and concentration dependent uptake in case of mannose conjugates. These results suggest a profound impact of the dye which should be taken into consideration when studying the uptake of small molecules by dye conjugation.
Subject(s)
Mannose/chemistry , Mannose/metabolism , A549 Cells , Biological Transport , Chemistry Techniques, Synthetic , Flow Cytometry , Humans , Mannose/chemical synthesis , Temperature
2.
Eur Respir J
; 54(1)2019 07.
Article
in English
| MEDLINE
| ID: mdl-30956203