ABSTRACT
A total of 200 patients with acute and chronic hepatic diseases and 100 apparently normal cross-matched controls were examined for the parasitic cause. The IHAT showed 3% had hydatid cysts, 3% had fascioliasis and 53.5% had schistosomiasis. However, no eggs were detected in the fascioliasis patients and only 4 & 3 cases showed S. mansoni and S. haematobium eggs in stools & urines respectively. ELISA showed 10% with visceral larva migrans & 65.5% with toxoplasmosis (IgG, IgM or both). On the other hand, 27% of the controls showed antibodies against toxoplasmosis.
Subject(s)
Liver Diseases, Parasitic/diagnosis , Liver Diseases, Parasitic/parasitology , Acute Disease , Animals , Antibodies, Helminth/blood , Antibodies, Protozoan/blood , Chronic Disease , Echinococcosis, Hepatic/diagnosis , Enzyme-Linked Immunosorbent Assay , Fasciola/immunology , Fascioliasis/diagnosis , Feces/parasitology , Female , Hemagglutination Tests , Humans , Liver Diseases, Parasitic/immunology , Male , Schistosoma japonicum/immunology , Schistosoma mansoni/immunology , Schistosomiasis/diagnosis , Toxoplasma/immunology , Toxoplasmosis/diagnosis , Urine/parasitologyABSTRACT
Out of 150 Egyptian bats (Pipistrellus Kuhli) hunted in the premises of Sohag City, 60 (40%) were harbouring typanosomes of the subgenus Megatypanum (Hoare, 1964). They were T. M. heybergi (Rodhian, 1923), T. M. possoai (Dean and Sugary, 1963) and T. M. magadermae (Wenyon, 1990).
Subject(s)
Chiroptera/parasitology , Trypanosoma/classification , Trypanosoma/isolation & purification , Animals , Egypt , Prevalence , Trypanosomiasis/parasitology , Trypanosomiasis/veterinaryABSTRACT
It can be safely said that up till now, no method of vaccination (including recent genes encoding vaccines) has yet proved to be totally effective since they gave partial and low levels of protection against S. mansoni infection. The objective of this work is to try testing the immunogenic effect of two purified non infected B. alexandrina hepatopancreas through histopathological changes in liver of Swiss albino mice (15-20 gm). Gel filtration chromatography was used to fractionate the crude antigen into five fractions followed by re-fractionation and determination of their molecular weights by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Four dilutions of Fiv (20000-29000 daltons) and Fv (20000-24000 daltons) were injected in two groups of mice (33 each) at weekly intervals and another control group was injected with phosphate buffer saline (PBS) in the same manner. Sacrification was done seven weeks from infection with 100 S. mansoni cercariae through immersion method. The results revealed that there is marked histopathological changes in liver of the control group in comparison to the two vaccinated groups which appear more or less normal with slight inflammatory infiltrate.