ABSTRACT
Amoxicillin was administered as a single subcutaneous injection at 12.5 mg/kg to four koalas and changes in amoxicillin plasma concentrations over 24 hr were quantified. Amoxicillin had a relatively low average ± SD maximum plasma concentration (Cmax ) of 1.72 ± 0.47 µg/ml; at an average ± SD time to reach Cmax (Tmax ) of 2.25 ± 1.26 hr, and an elimination half-life of 4.38 ± 2.40 hr. The pharmacokinetic profile indicated relatively poor subcutaneous absorption. A metabolite was also identified, likely associated with glucuronic acid conjugation. Bacterial growth inhibition assays demonstrated that all plasma samples other than t = 0 hr, inhibited the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 to some extent. Calculated pharmacokinetic indices were used to predict whether this dose could attain a plasma concentration to inhibit some susceptible Gram-negative and Gram-positive pathogens. It was predicted that a twice daily dose of 12.5 mg/kg would be efficacious to inhibit susceptible bacteria with an amoxicillin minimum inhibitory concentration (MIC) ≤ 0.75 µg/ml such as susceptible Bordetella bronchiseptica, E. coli, Staphylococcus spp. and Streptococcus spp. pathogens.
Subject(s)
Amoxicillin/pharmacokinetics , Anti-Bacterial Agents/pharmacokinetics , Phascolarctidae/metabolism , Amoxicillin/administration & dosage , Amoxicillin/blood , Animals , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/blood , Area Under Curve , Blood Proteins/metabolism , Chromatography, Liquid/veterinary , Escherichia coli/drug effects , Escherichia coli/growth & development , Female , Glucuronides/metabolism , Half-Life , Injections, Subcutaneous/veterinary , Male , Mass Spectrometry/veterinary , Protein Binding , Staphylococcus aureus/drug effects , Staphylococcus aureus/growth & developmentABSTRACT
OBJECTIVE: There has been a growing interest in finding plants with biological active ingredients for medicinal application. MATERIALS AND METHODS: Three colors of petals of Althaea officinalis (A. officinalis) flowers, i.e., pink, reddish pink, and white were examined for total antioxidant activity and ï¬avonoids content. RESULTS: The reddish pink flowers of A. officinalis have more antioxidant activity and the power of antioxidant activity was reddish pink > pink > white. CONCLUSION: Findings suggest that the dark color can serve as an indicator of antioxidant content of the plant. Flavonoid content was highest in white flower thus this result indicated that flowers with light color can be considered for medicinal uses.
