Thermography as an indicator of goat welfare in an intensive production system.

This study evaluated the welfare of Saanen, Moxoto, and Anglo-Nubian goats kept in collective or individual pens for a feedlot system, evaluated with infrared thermography. A total of twenty-four goats were used, eight for each breed. Animals were distributed in a completely randomized design, with a 2 × 3 factorial with two fixed effects: housing type (collective or individual pens) and breed (Moxoto, Saanen, and Anglo-Nubian). The surface temperature was evaluated using an infrared thermographic camera, and behavioral analysis was based on the qualitative behavior assessment using a fixed list of descriptors. The breed was not different for all behavior evaluations and surface temperature (p>0.05). There was a difference between the housing types, where the collective pens showed goats more agitated, frustrated, and sociable (p<0.05). There was an influence of agitated, apathetic, frustrated, attentive, and curious behaviors on surface temperatures, in which feet and body temperatures decreased in these goats. (p<0.05). Moxoto, Anglo-Nubian, and Saanen goats showed similar behavior even when kept in collective or individual pens. Individual pens can restrict the goats' social relationships but reduce negative behaviors such as irritation and frustration. The lower foot temperatures of feedlot goats are related to the attention behavior in 86.75% of the observations.

Ensiling Sorghum with Urea, Aerobic Exposure and Effects on Intake, Digestibility, Ingestive Behaviour and Blood Parameters of Feedlot Lambs.

This study was carried out to evaluate the effect of ensiling sorghum silage with urea and amending the aerobic exposure nutrients intake and apparent digestibility, ingestive behaviour and blood serum metabolites of feedlot lambs. Forty uncastrated crossbred Dorper × Santa Inês lambs, aged 150 ± 15 days and with an initial body weight of 21.73 ± 2.40 kg, were used. Animals were assigned in a 2 × 3 factorial arrangement. Thus, six silage diets were produced with various urea addition levels (UA: 0 and 5 g/kg on a natural matter basis) and periods of aerobic exposure of silages (PAE: 0, 24 and 48 h). An effect was observed for nutrient intakes of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre corrected for ash and protein (NDFap) and total digestive nutrients TDN (g/day) and for the total apparent digestibility of DM, OM and CP. There was an interaction effect between urea levels and aerobic exposure for ether extract (EE) and NDFap intakes (g/kg) and nonfibrous carbohydrate (NFC) digestibility (g/kg) (p = 0.012). The addition of 5 g/kg of urea to sorghum ensilage improved the digestibility parameters without changing dry matter intake and ingestive behaviour. The addition or not of urea does not change the blood parameters of the animals.

Chitosan associated with whole raw soybean in diets for Murrah buffaloes on ruminal fermentation, apparent digestibility and nutrients metabolism.

This study aimed to investigate the effects of chitosan and whole raw soybean on nutrient intake, apparent digestibility, nitrogen utilization, microbial protein synthesis, blood metabolites, feeding behavior, ruminal fermentation, digesta kinetics, and reticular flow of nutrients of buffaloes. Four ruminally-cannulated Murrah buffaloes (351 ± 15 kg of initial BW) were randomly assigned according to a 4 × 4 Latin square design. Treatments were arranged as 2 × 2 factorial arrangement: the first factor was whole raw soybean (WRS), and the second factor was chitosan (CHI) with or without their inclusion in diets. Intake and apparent digestibility of ether extract (p < .01; p = .04, respectively), non-fiber carbohydrates intake (p = .03) and apparent ruminal digestibility of dry matter (p = .01) were affected by diets. An interaction effect or tendency was observed for microbial nitrogen (p = .09), concentrations, ruminal ammonia nitrogen (p = .05), total volatile fatty acid (p = .03). Association of chitosan with whole raw soybean has potential effects as a modulator of rumen fermentation; therefore, chitosan can be applied as an alternative non-ionophore for Murrah buffaloes.

AVALIAÇÃO DO COMPORTAMENTO INGESTIVO DE NOVILHAS ¾ HOLANDÊS X ZEBU ALIMENTADAS COM SILAGEM DE CAPIM-ELEFANTE ACRESCIDA DE 10% DE FARELO DE MANDIOCA: ASPECTOS METODOLÓGICOS

O presente trabalho teve como objetivo definir o intervalo de tempo para o registro do comportamento ingestivo de novilhas ¾ holandês x xebu com doze meses de idade. Foram observadas quatro novilhas, durante dois períodos de 24 horas, na última semana do período experimental. As variáveis estudadas foram os tempos diários totais, o número de períodos e o tempo/período das atividades de alimentação, ruminação e ócio. Foram comparados os intervalos de 10, 15, 20, 25 e 30 minutos contra o intervalo de 5 minutos. Não houve diferenças significativas entre os tempos médios diários de alimentação, ruminação e ócio medidos nas diferentes escalas de tempo, indicando que este tipo de experimento pode ser feito com intervalos de até 30 minutos entre cada observação, sem comprometer os resultados, pois a média de tempo destas atividades ocorre em períodos discretos superiores a este intervalo médio. PALAVRAS-CHAVE: Alimentação, etologia, ócio, ruminação.

Avaliação do comportamento ingestivo de novilhas ¾ holandês x zebu alimentadas com silagem de capim-elefante acrescida de 10% de farelo de mandioca: aspectos metodológicos

O presente trabalho teve como objetivo definir o intervalo de tempo para o registro do comportamento ingestivo de novilhas ¾ holandês x xebu com doze meses de idade. Foram observadas quatro novilhas, durante dois períodos de 24 horas, na última semana do período experimental. As variáveis estudadas foram os tempos diários totais, o número de períodos e o tempo/período das atividades de alimentação, ruminação e ócio. Foram comparados os intervalos de 10, 15, 20, 25 e 30 minutos contra o intervalo de 5 minutos. Não houve diferenças significativas entre os tempos médios diários de alimentação, ruminação e ócio medidos nas diferentes escalas de tempo, indicando que este tipo de experimento pode ser feito com intervalos de até 30 minutos entre cada observação, sem comprometer os resultados, pois a média de tempo destas atividades ocorre em períodos discretos superiores a este intervalo médio. PALAVRAS-CHAVE: Alimentação, etologia, ócio, ruminação.

Hemoglobin H disease resulting from the association of the - alpha 3.7 rightward deletion and the (alpha alpha)MM deletion in a Brazilian patient.

A patient with Hb H disease resulting from the association of the - alpha 3.7 rightward deletion with the rare (alpha alpha)MM deletion, which removes the entire alpha-major regulatory element (MRE), is reported. This is the first description of an alpha-thalassemic mutation resulting from deletion of the locus-controlling sequences in the South-American population.

Capsular type distribution and susceptibility to antibiotics of Streptococcus pneumoniae clinical strains isolated from Uruguayan children with systemic infections. Pneumococcus Study Group.

Children under 24 months of age are at high risk for serious infection with Streptococcus pneumoniae but they do not elicit effective immune responses to the currently available capsular polysaccharide vaccines. A polysaccharide protein conjugated vaccine involving the most frequent types has become an urgent need. To produce such a vaccine for Latin America, information on type distribution is required. Recently, Uruguay was 1 of the 6 countries in Latin America where surveillance for invasive pneumococcal infections in children under the age of 5 years was carried out. Seventy percent of the 182 invasive S. pneumoniae isolates were recovered from patients under 24 months of age, and 19% were recovered from infants under 6 months. The 7 most frequent types were 14, 5, 1, 6B, 3, 7F, and 19A; representing 80% of invasive isolates. Twenty-one types were identified, 16 in pneumonia and 14 in meningitis. Resistance to penicillin increased during the study period, from 29% in 1994, to 40% in 1995-1996, mainly because of the spread of type 14 strains resistant to penicillin and trimethoprim/sulphamethoxazol (89% of resistant isolates). The high proportion of systemic pneumococcal infections recorded in patients under 24 months of age and the increasing resistance of these agents to first-choice antibiotics point to an urgent need for a capsular polysaccharide protein conjugated vaccine.

Atypical HbH disease in a Surinamese patient resulting from a combination of the -SEA and -alpha 3.7 deletions with HbC heterozygosity.

The first case of haemoglobin H (HbH) disease in combination with haemoglobin C (HbC) is reported in a man of Surinamese origin. Only haemoglobin A (HbA) and HbC were detected by electrophoresis. The amount of HbC was much less than expected in HbC heterozygotes. The synthesis ratio (beta A+ beta C/alpha) indicated an alpha-thalassaemia defect with two non-functional alpha genes, which did not correlate with the degree of haemolysis and anaemia displayed by the patient. The DNA analysis of the alpha-genes clusters revealed a defect combination -SEA/-alpha 3.7. The haematological data and the physiopathology of this atypical case are compared with the typical HbH disease found in a first cousin of the propositus. Data on the globin chains expression and on the formation of beta A and beta C homotetramers in HbH/HbC disease are presented.