ABSTRACT
Respiratory tract infections (RTIs) are among the most common and important problems in clinical medicine, making antibiotics the gold standard therapeutic option regardless of their frequent viral etiology. Their excessive and inappropriate use contributes to the rapid rise of antibiotic resistance and underscores the need for alternative strategies, especially when dealing with recurrent RTIs. Prevention is the ideal alternative, but specific vaccines targeting a wide range of respiratory pathogens are scarce. MV130 is a sublingual bacterial vaccine that induces trained immunity and provides non-specific protection against respiratory pathogens in various clinical settings according to the concept of TIbV (Trained Immunity-based Vaccine). A retrospective real-world study (RWS) was conducted to evaluate the annual incidence of RTIs and the consumption of antibiotics before and after the administration of MV130, using data sourced from the medical records of 599 patients (186 children and 413 adults) who suffered from recurrent RTIs. The median number of infectious episodes in children was significantly reduced by more than 70% from 5 episodes (interquartile range (IQR) 4.0-6.0) to 1 (IQR, 0.0-2.0) (p < 0.001) after MV130. Similarly, in adults, the median number of episodes before MV130 immunization was 5 (IQR, 4.0-6.0), which dropped by more than 80% to 1 (IQR, 0.0-1.0) during the year following MV130 immunization (p < 0.001). The median number of antibiotic courses also significantly decreased for both children and adults by over 80% (p < 0.001). This RWS showed that MV130 is an effective strategy for the prevention of respiratory infections and the reduction of associated antibiotic consumption.
ABSTRACT
No disponible
Subject(s)
Humans , Male , Infant , Bacteremia/diagnosis , Bacteremia/microbiology , Herbaspirillum/isolation & purification , Bacteremia/drug therapy , Herbaspirillum/drug effects , Gram-Negative Aerobic Rods and Cocci/isolation & purification , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSubject(s)
Bacteremia/microbiology , Catheter-Related Infections/microbiology , Gram-Negative Bacterial Infections/microbiology , Herbaspirillum/isolation & purification , Infant, Premature, Diseases/microbiology , Opportunistic Infections/microbiology , Cystic Fibrosis/blood , Cystic Fibrosis/diagnosis , Disease Susceptibility , Drug Resistance, Multiple, Bacterial , Drug Substitution , Ductus Arteriosus, Patent/surgery , Herbaspirillum/drug effects , Herbaspirillum/pathogenicity , Humans , Infant , Infant, Extremely Low Birth Weight , Infant, Newborn , Infant, Premature , Male , Postoperative Complications/microbiology , Trypsinogen/bloodABSTRACT
No disponible
Subject(s)
Humans , Female , Adult , Chorioamnionitis/microbiology , Actinomyces/pathogenicity , Neonatal Sepsis/diagnosis , Neonatal Sepsis/drug therapy , Fetal Diseases , Actinomycosis , Pregnancy ComplicationsSubject(s)
Actinomycosis/transmission , Neonatal Sepsis/etiology , Pregnancy Complications, Infectious/microbiology , Actinomyces/isolation & purification , Actinomycosis/drug therapy , Actinomycosis/microbiology , Adult , Anti-Bacterial Agents/therapeutic use , Chorioamnionitis/microbiology , Female , Gestational Age , Humans , Infant, Newborn , Male , Maternal-Fetal Exchange , Neonatal Sepsis/drug therapy , Neonatal Sepsis/microbiology , Obesity/complications , Pregnancy , Pregnancy Complications , Pregnancy Complications, Infectious/drug therapy , RecurrenceABSTRACT
PURPOSE: The aim of this study was to evaluate two non-commercial Real-Time PCR assays for the detection of microorganisms in amniotic fluid followed by identification by pyrosequencing. METHODS: We collected 126 amniotic fluids from 2010 to 2015 for the evaluation of two Real-Time PCR assays for detection of bacterial DNA in amniotic fluid (16S Universal PCR and Ureaplasma spp. specific PCR). The method was developed in the Department of Microbiology of the University Hospital La Paz. RESULTS: Thirty-seven samples (29.3%) were positive by PCR/pyrosequencing and/or culture, 4 of them were mixed cultures with Ureaplasma urealyticum. The Universal 16S Real-Time PCR was compared with the standard culture (81.8% sensitivity, 97.4% specificity, 75% positive predictive value, 98% negative predictive value). The Ureaplasma spp. specific Real-Time PCR was compared with the Ureaplasma/Mycoplasma specific culture (92.3% sensitivity, 89.4% specificity, 50% positive predictive value, 99% negative predictive value) with statistically significant difference (p=0.005). CONCLUSIONS: Ureaplasma spp. PCR shows a rapid response time (5h from DNA extraction until pyrosequencing) when comparing with culture (48h). So, the response time of bacteriological diagnosis in suspected chorioamnionitis is reduced.
