Morphomechanical reactions and mechanically stressed structures in amphibian embryos, as related to gastrulation and axial organs formation.

Reactions of embryonic tissues to a distributed and concentrated stretching are described and compared with the mechanics of the normal gastrulation movements. A role of mechanically stressed dynamic cell structures in the gastrulation, demarcation of notochord borders and in providing proportionality of the axial rudiments is demonstrated. A morphomechanical scheme of amphibian gastrulation is presented.

Local and global dynamics in collective movements of embryonic cells.

Several important morphogenetic processes belong to the category of collective cell movements (CCM), by which we mean coordinated rearrangements of many neighboring cells. The causes of the dynamic order established during CCM are still unclear. We performed statistical studies of rates and angular orientations of cell rearrangements in two kinds of embryonic tissues, which we categorized as "committed" (in the sense of being capable of autonomous CCM) as opposed to "naïve" tissues, which are those that require external forces in order to exhibit full scale CCM. In addition, we distinguished two types of cell rearrangements: first, those in which mutual cell-cell shifts characterizing the local dynamics (LD); and, second, those which moved in reference to common external coordinates (global dynamics, GD). We observed that in most cases LD rates deviated from normal distributions and do so by creating excesses of extensively converging and moderately diverging cells. In contrast, GD was characterized by nearly random behavior of slowly moving cells, combined with increased angular focusing of the fast cells trajectories as well as bimodal distribution of cell rates. When committed tissues were opposed by external mechanical forces, then they tended to preserve the inherent CCM patterns. On the other hand, the naïve ones reacted by creating two orthogonal cells flows, one of these coinciding with the force direction. We consider CCM as a self-organizing process based on feedbacks between converging and diverging cell shifts, which is able to focus the trajectories imposed by external forces.

Gastrulation in amphibian embryos, regarded as a succession of biomechanical feedback events.

Gastrulation in amphibian embryos is a composition of several differently located morphogenetic movements which are perfectly coordinated with each other both in space and time. We hypothesize that this coordination is mediated by biomechanical interactions between different parts of a gastrulating embryo based upon the tendency of each part to hyper-restore the value of its mechanical stress. The entire process of gastrulation in amphibian embryos is considered as a chain of these mutually coupled reactions, which are largely dependent upon the geometry of a given embryo part. We divide gastrulation into several partly overlapped steps, give a theoretical interpretation for each of them, formulate the experiments for testing our interpretation and describe the experimental results which confirm our point of view. Among the predicted experimental results are: inhibition of radial cell intercalation by relaxation of tensile stresses at the blastula stage; inversion of convergent intercalation movements by relaxation of circumferential stresses at the early gastrula stage; stress-promoted reorientation of axial rudiments, and others. We also show that gastrulation is going on under a more or less constant average value of tensile stresses which may play a role as rate-limiting factors. A macro-morphological biomechanical approach developed in this paper is regarded as complementary to exploring the molecular machinery of gastrulation.