Preliminary analysis of oral and gut microbiome of an elderly patient with late-diagnosed phenylketonuria.

Phenylketonuria (PKU) is a metabolic and genetic disorder caused by a phenylalanine hydroxylase (PAH) gene deficiency that raises Phe levels in organs. Dietary therapy involves an elimination diet and Phe-free items, which may alter microbiota. The study examined the oral and intestinal microbiomes of a 63-year-old PKU patient and a control man, living in rural areas. iSeq100 (Illumina) sequenced the stool and oral 16S rRNA gene V3-V4 region. PKU guts had more Firmicutes and fewer Bacteroidetes than control. Clostridia predominated in PKU, while Bacteroidia dominated in control. Oral Bacteroidetes. Firmicutes, Proteobacteria, and Fusobacteria phyla were similar in both men. The microbiome may differ from those fed a Phe-free diet from birth due to late diagnosis and treatment of PKU. Due to the age of the 63-year-old patient's and late therapy, the results differ from earlier studies. No study has compared an older PKU patient's gut and oral microbiomes.

Analysis of the Diet Quality and Nutritional State of Children, Youth, and Young Adults with Prader-Willi Syndrome: A Polish Multiple Case Study.

Given the lack of data on dietary quality in young individuals with Prader-Willi syndrome (PWS) in Poland, a multiple case study was conducted in which anthropometric measurements and 7-day dietary records were collected from 20 subjects with PWS. The study group consisted of 8 females and 12 males with a mean age of 14.8 years and a mean BMI of 21.6. Based on BMI analysis, five subjects were overweight, including two subjects who were obese. The study showed that 35% of the subjects had energy intakes above the recommended levels. Protein deficiency was found in one subject in the analyzed diets. However, fat intake was excessive in four subjects, and the majority exceeded the recommended intake of saturated fatty acids. Vitamin E and B12 deficiencies were found in 40% and 85% of the subjects, respectively. All subjects had inadequate intakes of vitamin D and iodine, while the majority had deficiencies in sodium and copper intakes. Calcium intake was deficient in 35% of the subjects. However, most subjects met recommendations for the intakes of other minerals, vitamins, and fiber. These findings confirm the suboptimal dietary patterns of Polish individuals with PWS, with deficits observed in the intake of certain vitamins and minerals.

Comparison of the Health Status of Vegetarians and Omnivores Based on Biochemical Blood Tests, Body Composition Analysis and Quality of Nutrition.

Many vegetarians are motivated by the health aspect of starting a plant-based diet. This diet can offer many health benefits. The study aimed to check whether people on a vegetarian diet are in good health, have a good nutritional intake, and follow the principles of healthy eating compared with omnivores. Twenty-two vegetarians on a vegetarian diet for more than five years and 22 omnivores aged 18-45 were interviewed. Each of them was given a food questionnaire, body composition analysis (BIA), and biochemical blood analysis, and their 7-day diet was analyzed. Polish vegetarians exhibit similar health statuses and tend towards better health than omnivores. They have an adequate body composition. Biochemical blood analysis showed no significant differences in blood parameters between vegetarians and omnivores, despite specific deficiencies such as vitamin B12, vitamin D and elevated homocysteine levels in vegetarians. They have a better nutritional status and follow good dietary principles. However, they were more likely to consume alcohol, add salt to their meals and sweeten hot drinks. In addition, the lifestyle of vegetarians can be described as healthier, as they are more likely to engage in leisure-time physical activity and get enough sleep.

Apple Peel Supplementation Potential in Metabolic Syndrome Prevention.

(1) Introduction: Apples are a source of bioactive substances, e.g., anthocyanidins and flavonols, and dietary fiber. Their highest concentrations are observed in the skin. Metabolic syndrome (MetS) is a set of conditions originally associated with obesity. Excessive adipose tissue accompanying obesity leads to chronic inflammation and metabolic disorders, which result in the development of dyslipidemia, elevated blood pressure, and glucose levels. Thus, supplementation of apple peels, a source of antioxidant substances and fiber, could potentially be a method supporting the prevention of MetS. This paper summarizes the results of available research on the potential impact of apple peel supplementation on the components of MetS. (2) Results: The results from in vitro and animal model studies indicate a positive effect of apple peel supplementation on lipid profile, glucose levels, and blood pressure regulation mediators. Only one human study was performed, and it showed that the consumption of apple peels had an effect on endothelial function but not on other clinical parameters. At the moment, there are no results from observations on large groups of people available. (3) Conclusions: The results of in vitro and animal-model studies indicate the potential of apple peel supplementation in MetS prevention, but it has not been clinically confirmed in human studies. Conducting large human studies could allow a definite clarification of the role of apple peel supplementation in MetS prevention.

Changes in Novel Anthropometric Indices of Abdominal Obesity during Weight Loss with Selected Obesity-Associated Single-Nucleotide Polymorphisms: A Small One-Year Pilot Study.

Whether BMI and the competing waist circumference (WC)-based anthropometric indices are associated with obesity-related single-nucleotide polymorphisms (SNPs) is as yet unknown. The current study aimed to evaluate the anthropometric indices (fat mass index, body shape index, visceral adiposity index, relative fat mass, body roundness index, and conicity index) during a weight loss intervention in 36 obese individuals. Blood biochemical parameters (total cholesterol, low-density lipoprotein, high-density lipoprotein, and triglycerides) and three SNPs (FTO rs9939609, TFAP2B rs987237, and PLIN1 rs894160) were assessed in 22 women and 14 men (35.58 ± 9.85 years, BMI 35.04 ± 3.80 kg/m2) who completed a 12-month balanced energy-restricted diet weight loss program. Body composition was assessed via bioelectrical impedance (SECA mBCA515). At the end of the weight loss intervention, all anthropometric indices were significantly reduced (p < 0.05). For the SNP FTO rs9939609, the higher risk allele (A) was characteristic of 88.9% of the study group, in which 10 participants (27.8%) were homozygous. We found a similar distribution of alleles in TFAP2B and PLIN1. Heterozygous genotypes in FTO rs9939609 and TFAP2B rs987237 were predisposed to significant reductions in WC-based novel anthropometric indices during weight loss. The influence of PLIN1 rs894160 polymorphisms on the changes in the analyzed indices during weight loss has not been documented in the present study.

The Long-Term Dietitian and Psychological Support of Obese Patients Who Have Reduced Their Weight Allows Them to Maintain the Effects.

The role of post-therapeutic support after weight loss in obesity treatment is not fully understood. Therefore, weight maintenance after a successful weight loss intervention is not very common, especially in obese individuals. This randomized controlled study was conducted to explore the efficacy of following dietary and psychological support in a group of 36 obese individuals. Participants (22 women, 14 men aged 35.58 ± 9.85 years, BMI 35.04 ± 3.80 kg/m2) who completed a 12-month weight loss phase (balanced energy-restricted diet) were randomly allocated to receive 18-month support (SG) or no additional care (CG). The support phase included some elements of Ten Top Tips (TTT), cognitive behavioral therapy (CBT), motivational interviewing (MI) in combination with nutritional education and assessment of the level of physical activity. The primary outcome was the maintenance of anthropometric parameters at an 18-month follow-up. The secondary outcomes included evaluation of biochemical parameters and single nucleotide polymorphisms (SNPs) in genes connected with obesity. A comparison of SG vs. CG after a 30-month period of the study revealed significant differences in weight changes (-3.83 ± 6.09 vs. 2.48 ± 6.24 kg), Body Mass Index (-1.27 ± 2.02 vs. 0.72 ± 2.12 kg/m2), visceral adipose tissue (-0.58 ± 0.63 vs. 0.45 ± 0.74 L), and waist circumference (-4.83 ± 4.05 vs. 1.83 ± 5.97 cm). Analysis of SNPs (rs9939609 FTO, rs987237 TFAP2B, and rs894160 PLIN1) provided further insight into the potential modulating effect of certain genotypes on weight loss and maintenance and extended the knowledge of the potential benefits of personalized medicine. Post-therapeutical support in current clinical practice may increase the chances of long-term weight loss maintenance in obesity treatment even in patients with a genetic predisposition to excessive weight.

Organic food and health.

The popularity of organic foods grows systematically. In the last decade, several critical reviews and meta-analysis concerning organic food consumption and their effect on some chosen health problems have been published. The aim of the work was to present the current state of knowledge regarding the influence of organic food consumption on human health. On average, organic food of plant origin is characterized by a trace presence of pesticides, a lower content of nitrates and an increased content of polyphenols and vitamin C. Organic products of animal origin contain more beneficial for health unsaturated fatty acid. Organic dairy products, in contrast to meat products, are characterized by a higher content of protein and saturated fatty acids, however, the differences more result from the length of the grazing period and access to fresh forage than to the production system. Although generally, the consumption of organic food does not provide a significant nutritional advantage compared to a conventional diet, regular and frequent consumption of organic products generally reduces the risk of overweight and obesity, both for women and men, as well as non-Hodgkin lymphoma in case of women. Besides those, consumption of organic fruits and vegetables, as well as dairy products significantly reduces the risk of pre-eclampsia in pregnancy and eczema in infants, respectively. Positive effect on selected health problems probably results from a reduced amount of pesticide residues and an increased secondary plant metabolites intake which characterize organic food. This review showed that there is a need for further, especially, large cohort studies concerning the effect of organic food consumption on specific diseases development.

Chemical stability of fructans in apple beverages and their influence on chronic constipation.

The aim of this study was to analyse the concentration of reducing sugars in beverages based on apple juice with the addition of 2 and 4% of native and high polymerized inulin as well as oligofructose. Moreover, the effect of the consumption of this potentially prebiotic beverage containing highly polymerized inulin (12 g per 300 mL) on constipation was analysed. Pasteurization of the studied beverages followed by 120-day storage at ambient temperature, carried out in three independent trials, did not cause the hydrolysis of fructans into reducing sugars. Sensory analysis showed that the presence of fructans in beverages based on apple juice did not change the colour, clarity, odour, flavour, sweetness and acidity in comparison to apple juice. A placebo-controlled, randomized study involving 20 volunteers of age 20-29 with symptoms related to chronic constipation showed that the consumption of juice enriched with highly polymerized inulin significantly (p≤ 0.05) increased the frequency of bowel movements and facilitated defecation. The final conclusion is that fructans in beverages based on apple juice are chemically stable, do not affect sensory sensation and can help those with chronic constipation.

Fast consumption increases the risk of overweight and obesity

Background: Overweight and obesity are a problem negatively affecting human health. Besides the excess of energy from food, development of overweight can also result from food preferences, the frequency of meals and the speed of eating. Objective: The aim of this study was to analyse the effect of eating habits and physical activity on the occurrence of overweight and obesity. Material and method: The questionnaire survey concerning eating habits and physical activity was conducted among adults aged 20-59 (n=420) in Lublin province (Poland). The subjects were divided into two groups ­ normal (BMI 18.5-25 kg/m2, n=250) and overweight and obese (BMI≥25 kg/m2, n=170). One-way analysis of variance (ANOVA) and post-hoc Tukey's test as well as chi-square independence test were applied. In addition, the relative risk of overweight for groups divided according to their habits was determined. Results: The analysis of speed of eating was on the basis of subjective assessment of the subjects and as a relative speed of eating compared to family members and friends. In both methods of assessment, it has been shown that overweight and obesity facilitates fast food intake rate (p=0.0078 and p=0.0010, respectively) The relative risk of obesity and overweight increases almost twice (RR 1.79) when the number of meals consumed daily is between one and two compared to those having five meals a day. In addition, it has been shown that overweight facilitates low physical activity. Conclusions: Slowly consumed meals, high physical activity and having more than two meals a day promotes maintaining a normal body weight.

Nutrition of vegetarians in Poland ­ a review of research

The first mention of a vegetarian diet in Poland appeared in the 19th century. Since then, its popularity has been growing steadily year by year. Nevertheless, it remains a controversial topic. Many scientists try to explain what is the correct vegetarian diet and how its use can affect the health and proper human body functioning. Vegetarian diet is mainly based on the elimination of meat consumption. Depending on the type, it also involves the abandonment of fish, eggs as well as milk and their products. Such kind of nutrition based mainly on plant-derived products can effectively prevent diseases of civilization, reduce the risk of developing obesity, promote the maintenance of normal cholesterol and blood sugar, and lower blood pressure. However, an incorrectly balanced vegetarian diet may be associated with deficiencies of some vitamins and minerals. This paper reviews a few dozens of studies on people using a vegetarian diet in Poland. This review shows that vegetarians in Poland are not very well studied group and further research is needed.

Effect of different tea extracts on the physicochemical and sensory parameters of stirred probiotic yoghurts.

BACKGROUND: In yoghurt production, additives and flavours are added after the fermentation process, but in the case of yoghurts with the addition of tea extracts all studies concern set-type yoghurts. For this reason, the aim of this study was to analyse the effect of green, black, red (Pu-erh) and white tea extracts addition on sensory characteristics, pH, viscosity and colour of stirred probiotic yoghurts stored for two weeks. METHODS: Skim milk powder (10% w/w), sucrose (4% w/w) and typical yoghurt cultures with the addition of Lactobacillus acidophilus LA-5 were applied for yoghurt manufacture. Then yoghurts were stirred and 4, 8, 12% black, red, green and white tea extracts were added. The samples were analysed after 1, 3, 7 and 14 days of storage at 5ºC. Instrumental colour (L*a*b*) determination, rheological measurements and sensory evaluation were carried out. RESULTS: The pH analysis of the final yoghurt samples showed that the type and quantity of tea extract addition had little effect. Apparent viscosity was affected by 12% addition of tea extracts but not by storage time. The instrumental analysis of the colour confirmed the visual assessment. Sensory evaluation revealed that yoghurts with the addition of green tea extract were the most accepted. The addition of extracts made of red, black and white tea resulted in lower values of all the sensory characteristics tested. CONCLUSIONS: The stirring method can be applied to the production of probiotic yoghurts with various tea extracts. The acidity of the yoghurts was practically not affected by the addition of the infusion derived from the different types of tea. 4% addition of tea extract did not affect the viscosity of the yoghurts. The best sensory scores among tea yoghurts were obtained by yoghurt with 4% green tea addition.

Changes in Sedentary and Active Lifestyle, Diet Quality and Body Composition Nine Months after an Education Program in Polish Students Aged 11⁻12 Years: Report from the ABC of Healthy Eating Study.

The sustainability of education focused on improving the dietary and lifestyle behaviours of teenagers has not been extensively studied. The aim of this study was to determine the sustainability of diet-related and lifestyle-related school-based education on sedentary and active lifestyle, diet quality and body composition of Polish pre-teenagers in a medium-term follow-up study. An education-based intervention study was carried out on 464 students aged 11⁻12 years (educated/control group: 319/145). Anthropometric measurements were taken and body mass index (BMI) and waist-to-height ratios (WHtR) were calculated, both at the baseline and after nine months. Dietary data from a short-form food frequency questionnaire (SF-FFQ4PolishChildren) were collected. Two measures of lifestyle (screen time, physical activity) and two diet quality scores (pro-healthy, pHDI, and non-healthy, nHDI) were established. After nine months, in the educated group (vs. control) a significantly higher increase was found in nutrition knowledge score (mean difference of the change: 1.8 points) with a significantly higher decrease in physical activity (mean difference of the change: -0.20 points), nHDI (-2.3% points), the z-WHtR (-0.18 SD), and the z-waist circumference (-0.13 SD). Logistic regression modelling with an adjustment for confounders revealed that after nine months in the educated group (referent: control), the chance of adherence to a nutrition knowledge score of at least the median was over 2 times higher, and that of the nHDI category of at least the median was significantly lower (by 35%). In conclusion, diet-related and lifestyle-related school-based education from an almost one-year perspective can reduce central adiposity in pre-teenagers, despite a decrease in physical activity and the tendency to increase screen time. Central adiposity reduction can be attributed to the improvement of nutrition knowledge in pre-teenagers subjected to the provided education and to stopping the increase in unhealthy dietary habits.

The effect of replacing pork fat of inulin on the physicochemical and sensory quality of guinea fowl pate.

BACKGROUND: Potentially, pâtés could be a functional food and are an important source of proteins, vitamin A, B complex vitamins and iron. Unfortunately, one problem in pâtés is the high level of animal fat, about 30%. Pâté with low-fat guinea fowl meat and animal fat replaced with inulin can result in this product being classified as a pro-health food. The objective of the present study was to determine the effect on pâté with guinea fowl meat of reducing its pork back fat content (about 1/3, 2/3 and 100%) and the adding inulin as a partial fat substitute. The effects on the pâté's chemical and physicochemical composition, as well as on its textural characteristics and sensory properties were analysed. METHODS: On the day after production, the following took place: chemical analysis: cooking loss, moisture, protein, total fat, total calories, the pH, lipid oxidation were analysed; physical analysis: colour parameters, texture profile analysis and sensory evaluation were analysed. RESULTS: The pâté prepared with inulin gels as fat replacers had a fat content reduced (up to 82%), and decreased (up to 58%) energy value. The fat reduction and addition of inulin gels decreased hardness and chewiness, but the pâté's appearance, taste and odour, as well as overall quality were similar to the control (full-fat samples). CONCLUSIONS: The study demonstrated that inulin can be used in guinea fowl pâtés as a total fat replacer and a potential source of prebiotic.

A new insight into the physiological role of bile salt hydrolase among intestinal bacteria from the genus Bifidobacterium.

This study analyzes the occurrence of bile salt hydrolase in fourteen strains belonging to the genus Bifidobacterium. Deconjugation activity was detected using a plate test, two-step enzymatic reaction and activity staining on a native polyacrylamide gel. Subsequently, bile salt hydrolases from B. pseudocatenulatum and B. longum subsp. suis were purified using a two-step chromatographic procedure. Biochemical characterization of the bile salt hydrolases showed that the purified enzymes hydrolyzed all of the six major human bile salts under the pH and temperature conditions commonly found in the human gastrointestinal tract. Next, the dynamic rheometry was applied to monitor the gelation process of deoxycholic acid under different conditions. The results showed that bile acids displayed aqueous media gelating properties. Finally, gel-forming abilities of bifidobacteria exhibiting bile salt hydrolase activity were analyzed. Our investigations have demonstrated that the release of deconjugated bile acids led to the gelation phenomenon of the enzymatic reaction solution containing purified BSH. The presented results suggest that bile salt hydrolase activity commonly found among intestinal microbiota increases hydrogel-forming abilities of certain bile salts. To our knowledge, this is the first report showing that bile salt hydrolase activity among Bifidobacterium is directly connected with the gelation process of bile salts. In our opinion, if such a phenomenon occurs in physiological conditions of human gut, it may improve bacterial ability to colonize the gastrointestinal tract and their survival in this specific ecological niche.

Awareness of factors affecting osteoporosis obtained from a survey on retired Polish subjects.

BACKGROUND: Osteoporosis is a growing problem facing modern society and currently poses one of the most serious health challenges. It is a progressive skeletal disease characterised by low bone mineral density whose development depends on multiple factors. These principally include increasing age, nutrition, physical activity, endocrine changes, lifestyles, general health condition and taken drugs. OBJECTIVES: To assess how much subjects (aged > 50 years) are aware of the dangers in contracting osteoporosis along with the effects that lifestyle and dairy product consumption can have on this condition developing. MATERIAL AND METHODS: A questionnaire, designed in-house, was used to survey a group of 150 randomly selected individuals aged above 50 years. This was performed in June 2012. Questions were on socio-demographics, milk and dairy product consumption, physical activity as well as assessing knowledge about osteoporosis that included issues such as its incidence, prevention and morbidity. RESULTS: Osteoporosis was established in 60.7% subjects of whom 69.2% were women. Only 9.3% never consumed any dairy products. Physical activity of some kind was undertaken by 77.3% subjects. Within the last year, 38.0% reported having fractures of whom 46.0% had osteoporosis and 25% were healthy. Respondents were aware that consuming dairy products is beneficial to bone health and this awareness was higher among those with osteoporosis. Physical activity was also recognised to be important in preventing this condition. CONCLUSIONS: Subjects suffering from osteoporosis had twice as many more fractures than healthy subjects.

Factors affecting inulin crystallization after its complete dissolution.

In this study, we analyzed inulin crystallization during one year after its complete dissolution and an effect of inulin crystal seeds concentration on rheological and textural properties of inulin gels. 20% and 25% solutions of three different inulins, one native and two high performance (crystal and amorphous), were prepared by heating at 100°C for 5 min. During one year of storage at 20°C, inulin did not form a gel structure, but only precipitates and a crystal layer on the walls of the containers. Addition of crystal seeds (0.02-2%) caused formation of gel structure. Minimal concentration of the crystal seeds necessary to form a strong inulin gel was 0.4%. Crystallographic structure of inulin powder did not have an influence on the formed gels. The obtained results allow inulin gelation to control which can be crucial in novel foods, the structure of which is based on inulin.

Determining the effect of consuming fermented milk drinks on the incidence of constipation, diarrhoea and resistance to respiratory illness.

BACKGROUND: At present, there are many types of milk fermented drinks/beverages on the market that often contain added probiotic microflora. Numerous studies indicate that such products are frequently consumed by adolescents. Within this group, symptoms of respiratory illness feature prominently as well as various and frequent disorders of the gastro-intestinal system that give rise to either constipation or diarrhoea. OBJECTIVES: To determine the effect of consuming fermented milk drinks on the immune systems in teenagers aged 13-16 years through analysing their intakes in relation to how often respiratory illness, constipation and diarrhoea had occurred. MATERIAL AND METHODS: A survey was performed on 150 middle-school pupils aged 13-16 years. Correlation coefficients were determined between the frequency of consuming fermented drinks with the incidences of respiratory disease, constipation or diarrhoea. RESULTS: Most subjects declared that their state of health was satisfactory and that they regularly ate meals. Those consuming fermented milk drinks for over a year constituted 88%. It was found that 56% of all subjects drank such beverages at least once daily or several times a week. The decrease observed in consuming these drinks resulted in increasing problems of bowel evacuation ranging from 3% in those drinking daily to 26% that drank less than once monthly. The incidence of respiratory tract illness was seen to rise whenever fewer fermented milk beverages were consumed. Analogous findings were seen also in the incidence of diarrhoea. Subjects also considered that such drinks improved health and yoghurts were the most frequent type of these drinks consumed. CONCLUSIONS: Gastro-intestinal function may be enhanced by regular consumption of fermented milk drinks together with a decreased incidence of respiratory tract illness. However, the effect of how often such drinks are consumed on the aforementioned conditions remains unresolved.