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J Eukaryot Microbiol ; 65(6): 820-827, 2018 11.
Article in English | MEDLINE | ID: mdl-29655313

ABSTRACT

Mitochondrial genome sequence of Vannella croatica (Amoebozoa, Discosea, Vannellida) was obtained using pulse-field gel electrophoretic isolation of the circular mitochondrial DNA, followed by the next-generation sequencing. The mitochondrial DNA of this species has the length of 28,933 bp and contains 12 protein-coding genes, two ribosomal RNAs, and 16 transfer RNAs. Vannella croatica mitochondrial genome is relatively short compared to other known amoebozoan mitochondrial genomes but is rather gene-rich and contains significant number of open reading frames.


Subject(s)
Amoebozoa/genetics , Genome, Mitochondrial/genetics , Mitochondria/genetics , Base Composition , Base Sequence , DNA, Mitochondrial/genetics , DNA, Mitochondrial/isolation & purification , DNA, Protozoan/genetics , Gene Order , Genes, Protozoan/genetics , Open Reading Frames/genetics , Protozoan Proteins/genetics , RNA, Ribosomal/genetics , RNA, Transfer/chemistry , RNA, Transfer/genetics , Sequence Analysis, DNA
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