Antioxidant activities, dietary nutrients, and yield potential of bitter gourd (Momordica charantia L.) lines in diverse growing environments.

The biotic and abiotic stresses cause a significant decline in the yield and fruit quality traits, including antioxidants and minerals, of bitter gourd when grown in open fields. Protected cultivation technology has emerged to minimize such stresses. We investigated the effect of diverse environments (hi-tech greenhouse, naturally ventilated polyhouse, insect-proof net-house, and open field) and breeding lines on earliness, yield potential, antioxidant activities, and dietary nutrients. In the GYT analysis, 12 treatment combinations involving four growing environments and three breeding lines of bitter gourd were examined. The 3-year study suggested that the cultivation of bitter gourd crops in an insect-proof net house (NH) showed superior performance in earliness, yield-attributing traits, antioxidant activities, and dietary nutrients, followed by a naturally ventilated polyhouse (NP). However, NH was on par with NP and significantly better than the open-field-grown crop. The GYT biplot analysis highlighted that the combinations of NH and Pusa Rasdar outperformed and were the most stable treatments for all the traits investigated, followed by NH in conjunction with S32 and S57 lines. This study suggests that growing bitter gourd in protected environments is the optimal strategy to achieve early market prices and improve the yield and nutritional quality of the fruits.

Multivariate Data Analysis Assisted Mining of Nutri-rich Genotypes from North Eastern Himalayan Germplasm Collection of Perilla (Perilla frutescens L.).

Understanding the nutritional diversity in Perilla (Perilla frutescens L.) is essential for selecting and developing superior varieties with enhanced nutritional profiles in the North Eastern Himalayan (NEH) region of India. In this study, we assessed the nutritional composition of 45 diverse perilla germplasm collected from five NEH states using standard protocols and advanced analytical techniques. Significant variability was observed in moisture (0.39-11.67%), ash (2.59-7.13%), oil (28.65-74.20%), protein (11.05-23.15%), total soluble sugars (0.34-3.67%), starch (0.01-0.55%), phenols (0.03-0.87%), ferric reducing antioxidant power (0.45-1.36%), palmitic acid (7.06-10.75%), stearic acid (1.96-2.29%), oleic acid (8.11-13.31%), linoleic acid (15.18-22.74%), and linolenic acid (55.47-67.07%). Similarly, significant variability in mineral content (ppm) was also observed for aluminium, calcium, cobalt, chromium, copper, iron, potassium, magnesium, manganese, molybdenum, sodium, nickel, phosphorus, and zinc. Multivariate analyses, including hierarchical clustering analysis (HCA) and principal component analysis (PCA), revealed the enriched nutritional diversity within the germplasm. Correlation analysis indicated significant positive and negative relationships between nutritional parameters, indicating potential biochemical and metabolic interactions present in the perilla seeds. TOPSIS-based ranking identified promising genotypes for functional foods, pharmaceuticals, and nutritional applications. This study provides a first in-depth report of the nutritional composition and diversity of perilla germplasm in the NEH region, thus aiding in the identification of superior varieties for food and nutritional diversification and security.

Modeling maize growth and nitrogen dynamics using CERES-Maize (DSSAT) under diverse nitrogen management options in a conservation agriculture-based maize-wheat system.

Agricultural field experiments are costly and time-consuming, and often struggling to capture spatial and temporal variability. Mechanistic crop growth models offer a solution to understand intricate crop-soil-weather system, aiding farm-level management decisions throughout the growing season. The objective of this study was to calibrate and the Crop Environment Resource Synthesis CERES-Maize (DSSAT v 4.8) model to simulate crop growth, yield, and nitrogen dynamics in a long-term conservation agriculture (CA) based maize system. The model was also used to investigate the relationship between, temperature, nitrate and ammoniacal concentration in soil, and nitrogen uptake by the crop. Additionally, the study explored the impact of contrasting tillage practices and fertilizer nitrogen management options on maize yields. Using field data from 2019 and 2020, the DSSAT-CERES-Maize model was calibrated for plant growth stages, leaf area index-LAI, biomass, and yield. Data from 2021 were used to evaluate the model's performance. The treatments consisted of four nitrogen management options, viz., N0 (without nitrogen), N150 (150 kg N/ha through urea), GS (Green seeker-based urea application) and USG (urea super granules @150kg N/ha) in two contrasting tillage systems, i.e., CA-based zero tillage-ZT and conventional tillage-CT. The model accurately simulated maize cultivar's anthesis and physiological maturity, with observed value falling within 5% of the model's predictions range. LAI predictions by the model aligned well with measured values (RMSE 0.57 and nRMSE 10.33%), with a 14.6% prediction error at 60 days. The simulated grain yields generally matched with measured values (with prediction error ranging from 0 to 3%), except for plots without nitrogen application, where the model overestimated yields by 9-16%. The study also demonstrated the model's ability to accurately capture soil nitrate-N levels (RMSE 12.63 kg/ha and nRMSE 12.84%). The study concludes that the DSSAT-CERES-Maize model accurately assessed the impacts of tillage and nitrogen management practices on maize crop's growth, yield, and soil nitrogen dynamics. By providing reliable simulations during the growing season, this modelling approach can facilitate better planning and more efficient resource management. Future research should focus on expanding the model's capabilities and improving its predictions further.

Elucidating the interactive impact of tillage, residue retention and system intensification on pearl millet yield stability and biofortification under rainfed agro-ecosystems.

Micronutrient malnutrition and suboptimal yields pose significant challenges in rainfed cropping systems worldwide. To address these issues, the implementation of climate-smart management strategies such as conservation agriculture (CA) and system intensification of millet cropping systems is crucial. In this study, we investigated the effects of different system intensification options, residue management, and contrasting tillage practices on pearl millet yield stability, biofortification, and the fatty acid profile of the pearl millet. ZT systems with intercropping of legumes (cluster bean, cowpea, and chickpea) significantly increased productivity (7-12.5%), micronutrient biofortification [Fe (12.5%), Zn (4.9-12.2%), Mn (3.1-6.7%), and Cu (8.3-16.7%)], protein content (2.2-9.9%), oil content (1.3%), and fatty acid profile of pearl millet grains compared to conventional tillage (CT)-based systems with sole cropping. The interactive effect of tillage, residue retention, and system intensification analyzed using GGE statistical analysis revealed that the best combination for achieving stable yields and micronutrient fortification was residue retention in both (wet and dry) seasons coupled with a ZT pearl millet + cowpea-mustard (both with and without barley intercropping) system. In conclusion, ZT combined with residue recycling and legume intercropping can be recommended as an effective approach to achieve stable yield levels and enhance the biofortification of pearl millet in rainfed agroecosystems of South Asia.

Triple-zero tillage and system intensification lead to enhanced productivity, micronutrient biofortification and moisture-stress tolerance ability in chickpea in a pearlmillet-chickpea cropping system of semi-arid climate.

Pearlmillet-chickpea cropping system (PCCS) is emerging as an important sequence in semi-arid regions of south-Asia owing to less water-requirement. However, chickpea (dry-season crop) faces comparatively acute soil moisture-deficit over pearlmillet (wet-season crop), limiting overall sustainability of PCCS. Hence, moisture-management (specifically in chickpea) and system intensification is highly essential for sustaining the PCCS in holistic manner. Since, conservation agriculture (CA) has emerged is an important climate-smart strategy to combat moisture-stress alongwith other production-vulnerabilities. Hence, current study comprised of three tillage systems in main-plots viz., Complete-CA with residue retention (CAc), Partial-CA without residue-retention (CAp), and Conventional-tillage (ConvTill) under three cropping systems in sub-plots viz., conventionally grown pearlmillet-chickpea cropping system (PCCS) alongwith two intensified systems i.e. pearlmillet-chickpea-fodder pearlmillet cropping system (PCFCS) and pearlmillet-chickpea-mungbean cropping system (PCMCS) in split-plot design. The investigation outcomes mainly focused on chickpea (dry-season crop) revealed that, on an average, there was a significant increase in chickpea grain yield under CAc to the tune of 27, 23.5 and 28.5% under PCCS, PCFCS and PCMCS, respectively over ConvTill. NPK uptake and micronutrient (Fe and Zn) biofortification in chickpea grains were again significantly higher under triple zero-tilled CAc plots with residue-retention; which was followed by triple zero-tilled CAp plots without residue-retention and the ConvTill plots. Likewise, CAc under PCMCS led to an increase in relative leaf water (RLW) content in chickpea by ~ 20.8% over ConvTill under PCCS, hence, ameliorating the moisture-stress effects. Interestingly, CA-management and system-intensification significantly enhanced the plant biochemical properties in chickpea viz., super-oxide dismutase, ascorbate peroxidase, catalase and glutathione reductase; thus, indicating their prime role in inducing moisture-stress tolerance ability in moisture-starved chickpea. Triple zero-tilled CAc plots also reduced the N2O fluxes in chickpea but with slightly higher CO2 emissions, however, curtailed the net GHG-emissions. Triple zero-tilled cropping systems (PCFCS and PCMCS) both under CAc and Cap led to a significant improvement in soil microbial population and soil enzymes activities (alkaline phosphatase, fluorescein diacetate, dehydrogenase). Overall, the PCCS system-intensification with mungbean (PCMCS) alongwith triple zero-tillage with residue-retention (CAc) may amply enhance the productivity, micronutrient biofortification and moisture-stress tolerance ability in chickpea besides propelling the ecological benefits under semi-arid agro-ecologies. However, the farmers should preserve a balance while adopting CAc or CAp where livestock equally competes for quality fodder.

High-value crops' embedded groundnut-based production systems vis-à-vis system-mode integrated nutrient management: long-term impacts on system productivity, system profitability, and soil bio-fertility indicators in semi-arid climate.

The current study identified two new climate-resilient groundnut-based cropping systems (GBCSs), viz., groundnut-fenugreek cropping system (GFCS) and groundnut-marigold cropping system (GMCS), with appropriate system-mode bio-compost embedded nutrient management schedules (SBINMSs) for semi-arid South Asia. This 5-year field study revealed that the GMCS along with leaf compost (LC) + 50% recommended dose of fertilizers (RDF50) in wet-season crop (groundnut) and 100% RDF (RDF100) in winter-season crop (marigold) exhibited the highest system productivity (5.13-5.99 t/ha), system profits (US$ 1,767-2,688/ha), and soil fertility (available NPK). Among SBINMSs, the application of 5 t/ha leaf and cow dung mixture compost (LCMC) with RDF50 showed the highest increase (0.41%) in soil organic carbon (SOC) followed by LC at 5 t/ha with RDF50 and RDF100. Legume-legume rotation (GFCS) had significantly higher soil microbial biomass carbon (SMBC) and soil microbial biomass nitrogen (SMBN) than legume-non-legume rotations (groundnut-wheat cropping system (GWCS) and GMCS). Among SBINMSs, the highest SMBC (201 µg/g dry soil) and SMBN (27.9 µg/g dry soil) were obtained when LCMC+RDF50 was applied to groundnut. The SMBC : SMBN ratio was the highest in the GWCS. LC+RDF50 exhibited the highest SMBC : SOC ratio (51.6). The largest increase in soil enzymatic activities was observed under LCMC+RDF50. Overall, the GMCS with LC+RDF50 in the wet season and RDF100 in the winter season proved highly productive and remunerative with better soil bio-fertility. SBINMSs saved chemical fertilizers by ~25%' in addition to enhanced system productivity and system profits across GBCSs in semi-arid regions of South Asia. Future research needs to focus on studying the potential of diversified production systems on water and environmental footprints, carbon dynamics, and energy productivity under semi-arid ecologies.

Seed Germination Ecology of Chenopodium album and Chenopodium murale.

Chenopodium album L. and Chenopodium murale L. are two principal weed species, causing substantial damage to numerous winter crops across the globe. For sustainable and resource-efficient management strategies, it is important to understand weeds' germination behaviour under diverse conditions. For the germination investigations, seeds of both species were incubated for 15 days under different temperatures (10−30 °C), salinity (0−260 mM NaCl), osmotic stress (0−1 MPa), pH (4−10), and heating magnitudes (50−200 °C). The results indicate that the germination rates of C. album and C. murale were 54−95% and 63−97%, respectively, under a temperature range of 10 to 30 °C. The salinity levels for a 50% reduction in the maximum germination (GR50) for C. album and C. murale were 139.9 and 146.3 mM NaCl, respectively. Regarding osmotic stress levels, the GR50 values for C. album and C. murale were 0.44 and 0.43 MPa, respectively. The two species showed >95% germination with exposure to an initial temperature of 75 °C for 5 min; however, seeds exposed to 100 °C and higher temperatures did not show any germination. Furthermore, a drastic reduction in germination was observed when the pH was less than 6.0 and greater than 8.0. The study generated information on the germination biology of two major weed species under diverse ecological scenarios, which may be useful in developing efficient weed management tactics for similar species in future agri-food systems.

No-Tillage with Residue Retention and Foliar Sulphur Nutrition Enhances Productivity, Mineral Biofortification and Crude Protein in Rainfed Pearl Millet under Typic Haplustepts: Elucidating the Responses Imposed on an Eight-Year Long-Term Experiment.

Yield limitation and widespread sulphur (S) deficiency in pearl-millet-nurturing dryland soils has emerged as a serious threat to crop productivity and quality. Among diverse pathways to tackle moisture and nutrient stress in rainfed ecologies, conservation agriculture (CA) and foliar nutrition have the greatest potential due to their economic and environmentally friendly nature. Therefore, to understand ammonium thiosulphate (ATS)-mediated foliar S nutrition effects on yield, protein content, mineral biofortification, and sulphur economy of rainfed pearl millet under diverse crop establishment systems, a field study was undertaken. The results highlighted that pearl millet grain and protein yield was significantly higher under no-tillage +3 t/ha crop residue mulching (NTCRM) as compared to no-tillage without mulch (NoTill) and conventional tillage (ConvTill), whereas the stover yield under NTCRM and ConvTill remained at par. Likewise, grain and stover yield in foliar S application using ATS 10 mL/L_twice was 19.5% and 13.2% greater over no S application. The sulphur management strategy of foliar-applied ATS 10 mL/L_twice resulted in significant improvement in grain protein content, protein yield, micronutrient fortification, and net returns (₹ 54.6 × 1000) over the control. Overall, ATS-mediated foliar S nutrition can be an alternate pathway to S management in pearl millet for yield enhancement, micronutrient biofortification and grain protein content increase under ConvTill, as well as under the new NTCRM systems.

Foliar nutrient supplementation with micronutrient-embedded fertilizer increases biofortification, soil biological activity and productivity of eggplant.

Micronutrient malnutrition or hidden hunger remains a major global challenge for human health and wellness. The problem results from soil micro- and macro-nutrient deficiencies combined with imbalanced fertilizer use. Micronutrient-embedded NPK (MNENPK) complex fertilizers have been developed to overcome the macro- and micro-element deficiencies to enhance the yield and nutritive value of key crop products. We investigated the effect of foliar applications of an MNENPK fertilizer containing N, P, K, Fe, Zn and B in combination with traditional basal NPK fertilizers in terms of eggplant yield, fruit nutritive quality and on soil biological properties. Applying a multi-element foliar fertilizer improved the nutritional quality of eggplant fruit, with a significant increases in the concentration of Fe (+ 26%), Zn (+ 34%), K (+ 6%), Cu (+ 24%), and Mn (+ 27%), all of which are essential for human health. Increasing supply of essential micronutrients during the plant reproductive stages increased fruit yield, as a result of improved yield parameters. The positive effect of foliar fertilizing with MNENPK on soil biological parameters (soil microbial biomass carbon, dehydrogenase, alkaline phosphatase) also demonstrated its capacity to enhance soil fertility. This study suggests that foliar fertilizing with a multi-nutrient product such as MNENPK at eggplant flowering and fruiting stages, combined with the recommended-doses of NPK fertilizers is the optimal strategy to improve the nutritional quality of eggplant fruits and increase crop yields, both of which will contribute to reduce micronutrient malnutrition and hunger globally.