ABSTRACT
The incidence of deep-vein thrombosis and the need for thromboprophylaxis following isolated trauma below the knee is uncertain. We have investigated this with a prospective randomised double-blind controlled trial using low molecular weight heparin with saline injection as placebo in patients aged between 18 and 75 years who had sustained an isolated fracture below the knee which required operative fixation. All patients had surgery within 48 hours of injury and were randomised to receive either the placebo or low molecular weight heparin for 14 days, after which they underwent bilateral lower limb venography, interpreted by three independent radiologists. Further follow-up was undertaken at two, six, eight and 12 weeks. A total of 238 patients fulfilled all the inclusion criteria, with 127 in the low molecular weight heparin group and 111 in the placebo group, all of whom underwent bilateral venography. There was no statistically significant difference in the incidence of deep-vein thrombosis between those patients treated with low molecular weight heparin or the placebo (p = 0.22). The number of deep-vein thromboses in the two groups was 11 (8.7%) and 14 (12.6%), respectively. Age and the type of fracture were significantly associated with the rate of deep-vein thrombosis (p = 0.001 and p = 0.009, respectively) but gender, comorbidities and the body mass index were not. The overall incidence of deep-vein thrombosis in this series was 11%. There was no clinical or statistical significant reduction in the incidence of deep-vein thrombosis with the use of thromboprophylaxis. However, we accept that owing to a cessation of funding, recruitment to this trial had to be ended prior to establishing the necessary sample size. Our results cannot, therefore, categorically exclude the possibility that low molecular weight heparin treatment could be beneficial. We recommend a further multicentre trial be undertaken to resolve this matter.
Subject(s)
Anticoagulants/therapeutic use , Dalteparin/therapeutic use , Fractures, Bone/surgery , Leg Injuries/surgery , Venous Thrombosis/prevention & control , Adult , Aged , Ankle Injuries/surgery , Double-Blind Method , Female , Humans , Male , Middle Aged , Phlebography , Postoperative Care/methods , Postoperative Complications/diagnostic imaging , Postoperative Complications/prevention & control , Tibial Fractures/surgery , Venous Thrombosis/diagnostic imaging , Young AdultABSTRACT
Cardiac sarcolemmal Na(+)/H(+) exchange is critical for the regulation of intracellular pH, and its activity contributes to ischemia-reperfusion injury. It has been suggested that the membrane phospholipid environment does not modulate Na(+)/H(+) exchange. The present study was carried out to determine the effects on Na(+)/H(+) exchange of modifying the endogenous membrane phospholipids through the addition of exogenous phospholipase D. Incubation of 0.825 U of phospholipase D with 1 mg of porcine cardiac sarcolemmal vesicles hydrolyzed 34 +/- 2% of the sarcolemmal phosphatidylcholine and increased phosphatidic acid 10.2 +/- 0.5-fold. Treatment of vesicles with phospholipase D resulted in a 46 +/- 2% inhibition of Na(+)/H(+) exchange. Na(+)/H(+) exchange was measured as a function of reaction time, extravesicular pH, and extravesicular Na(+). All of these parameters of Na(+)/H(+) exchange were inhibited following phospholipase D treatment compared with untreated controls. Passive efflux of Na(+) was unaffected. Treatment of sarcolemmal vesicles with phospholipase C had no effect on Na(+)/H(+) exchange. We conclude that phospholipase D-induced changes in the cardiac sarcolemmal membrane phospholipid environment alter Na(+)/H(+) exchange.
Subject(s)
Ion Transport/physiology , Myocardium/metabolism , Organelles/metabolism , Phospholipase D/metabolism , Sarcolemma/metabolism , Sodium-Hydrogen Exchangers/metabolism , Animals , Dose-Response Relationship, Drug , Hydrogen-Ion Concentration , Intracellular Membranes/metabolism , Ion Transport/drug effects , Membrane Lipids/metabolism , Organelles/drug effects , Permeability/drug effects , Phosphatidic Acids/biosynthesis , Phosphatidylcholines/metabolism , Phospholipase D/pharmacology , Phospholipids/metabolism , Sarcolemma/drug effects , Sodium/metabolism , Sodium/pharmacokinetics , Sodium-Hydrogen Exchangers/antagonists & inhibitors , SwineABSTRACT
Ischemic heart disease is a significant problem in the diabetic population. Animal models of diabetes show a paradoxical resistance to ischemic challenge. The present treatise will discuss the mechanics involved and the central role that Na+-H+ exchanger plays in this response to ischemic-reperfusion injury.
Subject(s)
Diabetes Mellitus/physiopathology , Myocardial Reperfusion Injury/physiopathology , Sodium-Hydrogen Exchangers/physiology , Animals , Diabetes Mellitus, Experimental/physiopathology , Humans , Myocardial Reperfusion Injury/metabolismSubject(s)
Osteitis Deformans , Bone and Bones/diagnostic imaging , Child , Female , Humans , Male , Osteitis Deformans/diagnostic imaging , RadiographyABSTRACT
Iron(III) complexation with 2,3-pyridinediol has been investigated with a view to ascertaining the structures of the complexes formed and examining the analytical potential of this biochemically important ligand.