ABSTRACT
Malassezia spp in skin microbiome scalp has been implicated in seborrheic dermatitis pathogenesis. Thus, treatment based in antifungal combined to topical keratolitic agents have been indicated as well as oral isotretinoin as it reduces the sebum production, glandular's size and possesses anti-inflammatory properties. This randomized, comparative and therapeutic trial aimed toper form the genotypic identification of Malassezia species before and after low-dose oral isotretinoin or topical antifungal treatments for moderate to severe seborrhea and/or seborrheic dermatitis on scalp. Scales and sebum of the scalp were seeded in the middle of modified Dixon and incubated at 32°C. For genotypic identification polymerase chain reaction primers for the ITS and D1/D2 ribossomal DNA were used and followed by samples sequencing. The procedure was conducted before and after therapeutic and randomized intervention for moderate to severe seborrhea/seborrheic dermatitis on the scalp, including oral isotretinoin, 10 mg, every other day and anti-seborrheic shampoo (piroctone olamine), over six months. The M. globosa and M. restricta were the most frequent species isolated on the scalp before and after both treatments. Other non-Malassezia species were also identified. The Malassezia spp. were maintained in the scalp after both treatments that were equally effective for the control of seborrhea/seborrheic dermatitis clinical signs.
ABSTRACT
We performed a prospective double-blind study to evaluate the correlation between inhibition zones obtained by a disk-diffusion test, using Neo-sensitabs of fluconazole (Rosco Diagnostica), and the MICs generated by the NCCLS macrobroth dilution assay. Eighty clinical isolates, representing 5 of the clinically relevant species of Candida, were tested simultaneously by both methods. A clear inverse correlation was found between the results obtained by both tests (r = -0.69). In addition, there was high degree of agreement between methods in the identification of susceptible isolates. However, the resistance definition by disk-diffusion test had a positive predictive value of only 17%. Our data support the hypothesis that Rosco Fluconazole Neo-sensitabs have potential as a screening test for the identification of Candida isolates susceptible to fluconazole. Resistant isolates should be further investigated by standardized broth procedures.
Subject(s)
Antifungal Agents/pharmacology , Candida/drug effects , Candidiasis/microbiology , Fluconazole/pharmacology , Microbial Sensitivity Tests/methods , Double-Blind Method , Humans , Microbial Sensitivity Tests/standards , Prospective Studies , Quality Control , Reference ValuesABSTRACT
Thirteen essential oils were isolated from officinal plants and tested in vitro against dermatophyte strains isolated from patients with dermatophytosis. Of the tested oils, those obtained from Cinnamomum zeylanicum, Ocimum gratissimum, Cymbopogon citratus, Eugenia uniflora and Alpinia speciosa were found to be the most active, inhibiting 80% of the dermatophyte strains tested and producing inhibition zones more than 10 mm in diameter.
Subject(s)
Antifungal Agents/pharmacology , Arthrodermataceae/drug effects , Oils, Volatile/pharmacology , Plants, Medicinal , Microbial Sensitivity Tests , Species SpecificityABSTRACT
Four cases of human dermatophilosis observed in Rio de Janeiro, Brazil, are reported. Data that suggest nail infection by Dermatophilus congolensis are presented. The clinical spectrum of the disease ranged from an asymptomatic infection to a pustular eruption. Our findings suggest that epidermal Langerhans cells play a role in the pathogenesis of the infection.
Subject(s)
Actinomycetales Infections/pathology , Keratinocytes/pathology , Langerhans Cells/pathology , Skin Diseases, Bacterial/pathology , Actinomycetales Infections/microbiology , Adult , Female , Humans , Male , Microscopy, Electron , Middle Aged , Skin Diseases, Bacterial/microbiologyABSTRACT
E apresentado o primeiro caso brasileiro de feohifomicose subcutanea provocada por novo agente, Cladosporium elatum
Subject(s)
Middle Aged , Humans , Male , Cladosporium , MycosesABSTRACT
Os autores estudaram 22 pacientes portadores de moniliase vaginal recidivante submetidos ao tratamento pela metil-partricina por via oral e topica vaginal. Sao apresentados e discutidos os aspectos e resultados do tratamento simultaneo das formas vaginal recidivante e intestinal
