ABSTRACT
BACKGROUND AND OBJECTIVE: Tissue microarray (TMA) is a method of harvesting small disks of tissue from a range of standard paraffin tissue blocks and placing them in an array on a recipient paraffin block such that hundreds of cases can be analyzed simultaneously by using only a few microliters of antibody in immunohistochemistry as a single experiment. The TMA construction done with the help of automated tissue arrayer or commercially available rubber molds are expensive. This study involved the fabrication of TMA using rubber-based additional silicone mold constructed in the department and comparison of this method with two other methods of fabricating TMA. MATERIALS AND METHODS: The TMA mold was fabricated using silicone material in the department. The recipient blocks were prepared. The tissue core prepared from donor blocks were inserted into the recipient blocks. The sections taken from this were compared with the TMA using double-sided adhesive tape technique and TMA by punching out holes in prefabricated dummy paraffin recipient block for insertion of tissue core. RESULTS: The TMA using a mold made of silicone showed more advantages than other two methods. CONCLUSION: Fabricating TMA mold using silicone in the department is inexpensive and yet efficient.
ABSTRACT
Central giant cell granulomas (CGCG) are reddish lesions of gingiva that account for an important number of disorders frequently diagnosed in the regular dental practice. Although the majority of the lesions are nonaggressive, asymptomatic, and slow-growing, about 30% show an aggressive, progressively destructive behavior, and a tendency to recur. We present a case of aggressive CGCG of the maxilla in a 4-year-old female child managed by surgical excision. To minimize the possible cost of esthetic, functional, and psychological problems, mainly in young patients, CGCG should be diagnosed and managed at the earliest.