The inflammatory response to birth requires MyD88 and is driven by both mother and offspring.

Birth is an inflammatory event for the newborn, characterized by elevations in interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α peripherally and/or centrally, as well as changes in brain microglia. However, the mechanism(s) underlying these responses is unknown. Toll-like receptors (TLRs) play crucial roles in innate immunity and initiate inflammatory cascades upon recognition of endogenous or exogenous antigens. Most TLR signaling depends on the adaptor molecule myeloid differentiation primary response 88 (MyD88). We independently varied MyD88 gene status in mouse dams and their offspring to determine whether the inflammatory response to birth depends on MyD88 signaling and, if so, whether that signaling occurs in the offspring, the mother, or both. We find that the perinatal surges in plasma IL-6 and brain expression of TNF-α depend solely on MyD88 gene status of the offspring, whereas postnatal increases in plasma IL-10 and TNF-α depend on MyD88 in both the pup and dam. Interestingly, MyD88 genotype of the dam primarily drives differences in offspring brain microglial density and has robust effects on developmental neuronal cell death. Milk cytokines were evaluated as a possible source of postnatal maternal influence; although we found high levels of CXCL1/GROα and several other cytokines in ingested post-partum milk, their presence did not require MyD88. Thus, the inflammatory response previously described in the late-term fetus and newborn depends on MyD88 (and, by extension, TLRs), with signaling in both the dam and offspring contributing. Unexpectedly, naturally-occuring neuronal cell death in the newborn is modulated primarily by maternal MyD88 gene status.

Factors contributing to adolescents' COVID-19-related loneliness, distress, and worries.

As the world continues to face uncertainty due to COVID-19, there is concern for adolescent wellbeing as they attempt to cope with changes to their education and social interactions. Considering the trade-offs between physical distancing and virtual education versus in-person instruction may be particularly pertinent to understanding adolescent loneliness and worries surrounding COVID-19 and their academics. Adolescents (N = 1,708; age M = 12.42, SD = 2.40; 53.3% girls and 46.7% boys) from the United States self-reported their perceptions of social support, COVID-19 attitudes, and indicators of wellbeing (i.e., loneliness, COVID-19 stress, academic worry) in an online cross-sectional survey between November 30, 2020 to December 21, 2020. Higher levels of social support and attitudes of lower COVID-19 concern were associated with lower levels of loneliness, COVID-19 stress, and academic worries. Adolescents receiving greater virtual instruction were more worried about their academics compared to those receiving greater in-person instruction. A moderation effect between loneliness and instruction type revealed that the effect of social support was stronger for those receiving greater virtual instruction. Despite the quick and dramatic change to one's education, adolescents were relatively unaffected by the type of instruction they had recently received. Instead, perceived social support and their attitudes toward COVID-19 were more strongly related to their wellbeing.

Birth elicits a conserved neuroendocrine response with implications for perinatal osmoregulation and neuronal cell death.

Long-standing clinical findings report a dramatic surge of vasopressin in umbilical cord blood of the human neonate, but the neural underpinnings and function(s) of this phenomenon remain obscure. We studied neural activation in perinatal mice and rats, and found that birth triggers activation of the suprachiasmatic, supraoptic, and paraventricular nuclei of the hypothalamus. This was seen whether mice were born vaginally or via Cesarean section (C-section), and when birth timing was experimentally manipulated. Neuronal phenotyping showed that the activated neurons were predominantly vasopressinergic, and vasopressin mRNA increased fivefold in the hypothalamus during the 2-3 days before birth. Copeptin, a surrogate marker of vasopressin, was elevated 30-to 50-fold in plasma of perinatal mice, with higher levels after a vaginal than a C-section birth. We also found an acute decrease in plasma osmolality after a vaginal, but not C-section birth, suggesting that the difference in vasopressin release between birth modes is functionally meaningful. When vasopressin was administered centrally to newborns, we found an ~ 50% reduction in neuronal cell death in specific brain areas. Collectively, our results identify a conserved neuroendocrine response to birth that is sensitive to birth mode, and influences peripheral physiology and neurodevelopment.

An educational intervention for medical students to improve self-efficacy in firearm injury prevention counseling.

BACKGROUND: Most physicians support counseling patients about firearm injury prevention (FIP), but infrequently do so due to lack of training and low confidence. Interventions to increase counseling frequency should focus on improving physician self-efficacy. Firearm injuries affect many clinical specialties; therefore, trainees would benefit from early FIP education. This study aims to determine if a 20-min educational intervention improves self-efficacy in FIP counseling in third-year medical students. Knowledge and beliefs were also assessed as secondary indicators of self-efficacy. METHODS: This was a prospective study performed at a medical school associated with a tertiary care children's hospital during the 2016-17 academic year. Groups of 12-15 different third-year medical students were selected to receive either a 20-min intervention or control lecture during their monthly pediatric lectures. The intervention consisted of two clinical vignettes, a brief discussion about the importance of FIP, and suggestions for clinical integration. The control session was a case-based lecture about pediatric emergencies. Participants completed baseline electronic assessments. Intervention students also completed post-intervention assessments immediately following each session. All participants completed final assessments at 6 months. Data were analyzed using Wilcoxon signed-rank tests and Wilcoxon rank-sum. RESULTS: We surveyed a total of 130 students. Sixty-five students completed the entire series of assessments - 22 from the control and 43 from the intervention group. There were no significant differences between the control and intervention groups at baseline. Immediately after, intervention, participants reported feeling more self-efficacious, had improved knowledge of FIP risk factors, and had beliefs more consistent with providing FIP anticipatory guidance (p <  0.001 for all three measures). After 6 months, participants sustained improvement in one of two self-efficacy questions ("I feel ready to counsel patients about firearm injury prevention") and retained knowledge of risk factors (p <  0.05 for both). However, their beliefs did not significantly favor FIP counseling, and they were not more likely to engage in a conversation about firearm safety. CONCLUSIONS: A 20-min educational intervention acutely improved self-efficacy in FIP counseling in third-year medical students, but improvements weakened after six months. Without further training, the beneficial effects of a one-time intervention will likely wane with time.

Developmental trends in the process of constructing own- and other-race facial composites.

The current study examined developmental differences from the age of 5 to 18 in the creation process of own- and other-race facial composites. In addition, it considered how differences in the creation process affect similarity ratings. Participants created two composites (one own- and one other-race) from memory. The complexity of the composite creation process was recorded during Phase One. In Phase Two, a separate group of participants rated the composites for similarity to the corresponding target face. Results support the cross-race effect, developmental differences (based on composite creators) in similarity ratings, and the importance of the creation process for own- and other-race facial composites. Together, these findings suggest that as children get older the process through which they create facial composites becomes more complex and their ability to create facial composites improves. Increased complexity resulted in higher rated composites. Results are discussed from a psycho-legal perspective.

The effect of social dominance orientation on perceptions of corporal punishment.

Previous research has suggested the use of corporal punishment is widely endorsed in our society (Straus, 2000; Straus & Stewart, 1999). Furthermore, perceptions of what constitutes corporal punishment vary. The present study examined social dominance orientation (SDO) and age of child as potential factors that may influence perceptions of what is viewed as corporal punishment versus physical abuse. The sample consisted of 206 undergraduate students enrolled at a Rocky Mountain University. A series of regressions were used to examine the relationships between SDO and six forms of punishment. Findings suggest, higher levels of SDO are significantly related to more ratings of physical punishment versus physical abuse. The primary findings of the present study showed SDO was significantly related to how an individual perceives corporal punishment. These results have important implications by serving as a stepping-stone into further understanding what factors may have an influence on perceptions of corporal punishment.

Predictors of outcome following hip fracture rehabilitation.

OBJECTIVE: To determine the potential predictors of functional outcome after hip fracture rehabilitation in a large acute inpatient rehabilitation facility. DESIGN: Retrospective chart review study. SETTING: Large acute inpatient rehabilitation hospital. PARTICIPANTS: Patients admitted with a primary admitting diagnosis of hip fracture (n = 753). Patients were excluded if their hip fracture was the result of high-velocity trauma and if their stay was shorter than 48 hours (196 patients). METHODS: Independent variables included body mass index, gender, ethnicity, smoking history, alcohol consumption, past living situation, past ambulatory status, medical history, prealbumin level, medications that increase the risk of falling, and evidence of prior osteoporosis workup and treatment. These data were entered into a password-encrypted database. Univariate analyses were carried out to evaluate the relationship between independent variables and main outcomes, and multivariate analyses were performed to assess the impact of medical history of diabetes adjusting for other covariates. MAIN OUTCOME MEASUREMENTS: Discharge location; length of rehabilitation hospital stay (LOS); Functional Independence Measure (FIM) gain, which is calculated as the FIM(discharge) - FIM(admission); and length of stay efficiency (LOSE), which is calculated as the FIM gain divided by the LOS, and measures the rate of FIM change. RESULTS: Patients with diabetes had a worse LOSE (P = .0008). Multiple linear regression analysis revealed that patients who have a medical history of diabetes have a 0.33 reduction of LOSE compared with other patients. Predictors of better LOSE included younger age (P < .001), fewer medications that predispose to falls (P < .0001), and independent ambulation before fracture (P = .0003). CONCLUSION: We have found several significant patient characteristics that portend a better functional outcome after hip fracture. These include younger age, female gender, absence of diabetes mellitus, independent prefracture ambulation, not living alone before fracture, and being prescribed fewer medications that predispose to falling during rehabilitation. On the contrary, one of our most interesting findings is that patients with diabetes made slower gains, had a longer LOS and were less likely to be discharged directly home from the acute rehabilitation facility. There are numerous factors that may contribute to this, and suggestions are made for future research.