ABSTRACT
The effect of Rhodococcus erythropolis IMB Ac-5017 biosurfactants (surface-active substances, SAS) with different degree of purification on attachment of bacteria (Escherichia coli IEM-1, Bacillus subtilis BT-2, Proteus vulgaris BT-1, Staphylococcus aureus BMC-1, Pseudomonas aeruginosa P-55, Enterobacter cloacae AC-22, Erwinia aroidaeae B-433), yeasts (Candida albicans D-6) and fungi (Aspergillus niger P-3, Fusarium culmorum T-7) to the abiotic surfaces (glass, plastic, ceramics, steel, linoleum) was studied. The dependence of microorganisms adhesion on degree of SAS purification (supernatant, purified SAS solution), SAS concentration (0,04-1,25 mg/ml), type of surface and test-cultures was established. The adhesion of majority investigated bacterial cells after treatment of abiotic surfaces with supernatant of cultural liquid with SAS concentration 0,06-0,25 mg/ml was on the average 20-45, yeasts C. albicans D-6--30-75% and was less than that purified SAS solution with the same concentration. Higher antiadhesive activity of supernatant as compared to purified SAS solution testifies to possibility of exception of the expensive stage of isolation and purification at obtaining of preparations with antiadhesive properties.
Subject(s)
Bacterial Adhesion/drug effects , Culture Media, Conditioned/pharmacology , Rhodococcus/chemistry , Surface-Active Agents/pharmacology , Aspergillus niger/drug effects , Aspergillus niger/physiology , Bacillus subtilis/drug effects , Bacillus subtilis/physiology , Candida albicans/drug effects , Candida albicans/physiology , Dose-Response Relationship, Drug , Enterobacter cloacae/drug effects , Enterobacter cloacae/physiology , Erwinia/drug effects , Erwinia/physiology , Escherichia coli/drug effects , Escherichia coli/physiology , Fusarium/drug effects , Fusarium/physiology , Proteus vulgaris/drug effects , Proteus vulgaris/physiology , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/physiology , Rhodococcus/metabolism , Staphylococcus aureus/drug effects , Staphylococcus aureus/physiology , Surface Properties , Surface-Active Agents/isolation & purification , Surface-Active Agents/metabolismABSTRACT
Distributional peculiarities and the ultrastructure of multinuclear elastic cells have been studied in the developed thighbone of rat and rabbit fetuses and new-borns. Multinuclear chondro- and osteoclasts exhibit no noticeable structural specificity with respect to the resorption substrate and are considered to be one and the same type of clastic cells whose main function is to resorb the mineralized tissue.