ABSTRACT
Cold plasma technology has received significant attention in agriculture due to its effect on the seeds and plants of important cultivars, such as wheat. Due to climate change, wherein increasing temperatures and droughts are frequent, it is important to consider novel approaches to agricultural production. As increased dormancy levels in wheat are correlated with high temperatures and drought, improving the germination and root growth of wheat seeds could offer new possibilities for seed sowing. The main objective of this study was to evaluate the influence of direct (glow) and indirect (afterglow) radio-frequency (RF) oxygen plasma treatments on the germination of two winter wheat varieties: Apache and Bezostaya 1. The influence of plasma treatment on seed surface morphology was studied using scanning electron microscopy, and it was observed that direct plasma treatment resulted in a high etching and nanostructuring of the seed surface. The effect of plasma treatment on germination was evaluated by measuring the germination rate, counting the number of roots and the length of the root system, and the fresh weight of seedlings. The results of this study indicate that the response of seeds to direct and indirect plasma treatment may be variety-dependent, as differences between the two wheat varieties were observed.
ABSTRACT
BACKGROUND: In the present paper, a method for differentiation between common and spelt wheat grown in different farming systems (biodynamic, ecological, integrated, conventional), based on biomarkers identified from aqueous flour extracts (nitrogen and 14 soluble carbohydrates) was employed. RESULTS: The identification and determination of soluble carbohydrate content were carried out using gas chromatography-mass spectrometry, with the UV spectrum generated by mass spectrometry for comparison with the WILEY database. Soluble carbohydrates were determined in the peak area between 21.92 and 43.63 min-1 retention time. The obtained data set was analyzed by multivariate statistical techniques. It was revealed that common wheat exerted a much more pronounced tendency than spelt wheat to be influenced by the farming system. CONCLUSION: This differentiation was particularly well visualized after subjecting the data set to principal component analysis (PCA) and cluster analysis (CA). In the PCA graph, all spelt samples were positioned closer to the corresponding control sample, in contrast to the wheat samples, which were distributed over a huge area in the factor space. CA showed that the spelt samples grown under different farming systems were highly similar and grouped into one cluster. Common wheat samples cultivated under organic, biodynamic and integrated system were similar and represented the second cluster, whereas that cultivated under the conventional system was clearly separated from other samples. © 2020 Society of Chemical Industry.
Subject(s)
Biomarkers/analysis , Plant Extracts/chemistry , Triticum/chemistry , Triticum/growth & development , Carbohydrates/chemistry , Crop Production , Flour/analysis , Gas Chromatography-Mass Spectrometry , Mass Spectrometry , Principal Component Analysis , Triticum/classificationABSTRACT
The contents of sugars, organic acids, total phenolic content, and the antioxidant activity were quantified in the flesh of red beet from conventional (CON), integrated (INT), organic (ORG), biodynamic (BD), and control farming systems using established methods. Significant differences were measured for malic acid, total phenolic content (TPC), and total antioxidant activity, where malic acid content ranged from 2.39 g kg(-1) FW (control) to 1.63 g kg(-1) FW (CON, ORG, and INT). The highest TPC was measured in BD and control samples (0.677 and 0.672 mg GAE g(-1), respectively), and the lowest in CON samples (0.511 mg GAE g(-1)). Antioxidant activity was positively correlated with TPC (r2=0.6187) and ranged from 0.823 µM TE g(-1) FW to 1.270 µM TE g(-1) FW in CON and BD samples, respectively, whereas total sugar content ranged from 21.03 g kg(-1) FW (CON) to 31.58 g kg(-1) FW (BD). The importance of sugars, organic acids, phenols, and antioxidants for human health, as well as for plant resilience and health, gained from this explorative study, is discussed and put into perspective.