To Explore the Influencing Factors of Pericoronary Adipose Tissue and Noninvasive Fractional Flow Reserve on the Progression of Coronary Heart Disease Based on 320-Slice Coronary CTA.

BACKGROUND: The objective of the study was to measure pericoronal adipose tissue parameters, fractional flow reserve with coronary artery computed tomographic angiography (CTA), and difference of fractional flow reserve with coronary artery CTA, by using high-performance 320-slice coronary CTA combined with semiautomatic quantitative software and explore the relationship between them and progression of coronary artery disease. METHODS: According to the inclusion criteria, 118 patients with complete data were selected. According to the results of coronary angiography during follow-up review, the patients were divided into coronary artery disease progression group (n = 43) and coronary artery disease stable group (n = 75), and the clinical baseline data, pericoronal adipose tissue volume, pericoronal adipose tissue fat attenuation index, fractional flow reserve with coronary artery CTA, and difference of fractional flow reserve with coronary artery CTA were compared between the 2 groups. According to univariate and multivariate logistic regression analyses, the risk factors related to coronary artery disease progression were screened out from pericoronal adipose tissue parameters and noninvasive hemodynamic characteristics (fractional flow reserve with coronary artery CTA and difference of fractional flow reserve with coronary artery CTA). RESULTS: There was no significant difference in baseline clinical data between the progression group and the stable group (P >.05). The left anterior descending artery-fat attenuation index-40 mm, left anterior descending artery-fat attenuation index-70 mm, left circumflex artery-fat attenuation index-70 mm, right coronary artery-fat attenuation index-70 mm, and difference of fractional flow reserve with coronary artery CTA in the progression group were higher than those in the stable group, while fractional flow reserve with coronary artery CTA was lower than that in the stable group, and the differences were statistically significant (P <.05). After adjusting for several factors, the results showed that left anterior descending artery-fat attenuation index-40 mm (P =.002; odds ratio = 1.237; 95% CI: 1.081-1.415), right coronary artery-fat attenuation index-70 mm (P =.039; odds ratio = 1.119; 95% CI: 1.006-1.246), fractional flow reserve with coronary artery CTA (P =.001; odds ratio = 0.708; 95% CI: 0.581-0.846), and difference of fractional flow reserve with coronary artery CTA (P <.001; odds ratio = 1.846; 95% CI: 1.394-2.445) were related to the progression of coronary artery disease. Compared with the above 5 indicators, the area under curve (AUC) of the above indicators combined is larger (0.897). CONCLUSIONS: Quantitative pericoronal adipose tissue parameters and noninvasive hemodynamic characteristics based on 320-slice coronary CTA can be used as the basis for predicting the progression of coronary artery disease.

Non-tuberculosis extensive abdominal lymph node calcification leading to portal hypertension with esophageal and gastric variceal bleeding: a rare case report.

BACKGROUND: Calcification of abdominal lymph node is a common clinical phenomenon, but it is extremely rare to cause serious adverse clinical outcomes. In the present case, the ruptured hemorrhage of the oesophagogastric fundic varices occurred as a result of portal hypertension due to compression of the portal vein by calcified lymph nodes. The patient was treated with medication, interventional therapy, endoscopic therapy, and surgery, respectively and the four different treatment options for the bleeding are worth summarizing. The etiology of this case is extremely rare and is the first to be reported in the world. CASE PRESENTATION: A 32-year-old male patient with no apparent causes of sudden onset of vomiting of blood, the patient underwent four different treatment methods to stop the hemorrhage. The combined diagnosis of whole abdomen enhanced CT and angiography was calcified abdominal lymph nodes compressing the portal vein, leading to portal hypertension and resulting in esophageal and gastric variceal bleeding. Postoperatively, a biopsy of the caseous tubercular tissue of the abdominal wall observed intraoperatively was performed and the biopsy did not show a tubercular component. Therefore, the extensive intra-abdominal lymph node calcification was not associated with tuberculosis. The patient's bleeding ceased after surgery. CONCLUSION: This case has improved the clinician's understanding of the etiology of non-cirrhotic portal hypertension. Based on this, and with this case, the differences between various hemostatic measures were studied in depth.

Analysis of the Influence of Ferromagnetic Material on the Output Characteristics of Halbach Array Energy-Harvesting Structure.

Due to the particular arrangement of permanent magnets, a Halbach array has an significant effect of magnetism and magnetic self-shielding. It can stretch the magnetic lines on one side of the magnetic field to obtain an ideal sinusoidal unilateral magnetic field. It has a wide application range in the field of energy harvesting. In practical applications, magnetic induction intensity of each point in magnetic field is not only related to the induced current and conductor but also related to the permeability of the medium (also known as a magnetic medium) in the magnetic field. Permeability is the physical quantity that represents the magnetism of the magnetic medium, which indicates the resistance of magnetic flux or the ability of magnetic lines to be connected in the magnetic field after coil flows through current in space or in the core space. When the permeability is much greater than one, it is a ferromagnetic material. Adding a ferromagnetic material in a magnetic field can increase the magnetic induction intensity B. Iron sheet is a good magnetic material, and it is easy to magnetize to generate an additional magnetic field to strengthen the original magnetic field, and it is easy to obtain at low cost. In this paper, in order to explore the influence of ferromagnetic material on the magnetic field and energy harvesting efficiency of the Halbach array energy harvesting structure, iron sheets are installed on the periphery of the Halbach array rotor. Iron sheet has excellent magnetic permeability. Through simulation, angle between iron sheet and Halbach array, radian size of iron sheet itself and distance between iron sheet and Halbach array can all have different effects on the magnetic field of the Halbach array. It shows that adding iron sheets as a magnetic medium could indeed change the magnetic field distribution of the Halbach array and increase energy harvesting efficiency. In this paper, a Halbach array can be used to provide electrical power for passive wireless low-power devices.

Is CSF1R Expression Localization Crucial for its Prognostic Value in Colorectal Cancer?

The colony-stimulating factor 1 receptor (CSF1R) is commonly known as a transmembrane receptor on tumor-associated macrophages, which are essential in the tumor microenvironment. However, the expression pattern and prognosis of CSF1R are still unknown in colorectal cancer (CRC) and are still controversial among various cancers. To clarify the expression pattern and prognosis of CSF1R in CRC, we performed immunohistochemistry on 332 CRC tissue and 283 nontumorous adjacent tissues. We explored the expression pattern of CSF1R and analyzed its relationship with clinical characteristics and prognosis. The positive expression ratio of CSF1R was much higher in nontumorous adjacent tissues, while the positive cytomembrane ratio of CSF1R was much higher in cancer tissue. Furthermore, we found that CSF1R expression, especially in the cytoplasm, acted as a protective factor. However, our findings indicated that CSF1R expression on the cytomembrane decreased its prognostic predictive value. These results emphasize the important role of the localization of CSF1R and may also explain the contrasting effects seen between various cancers.

HIP1R acts as a tumor suppressor in gastric cancer by promoting cancer cell apoptosis and inhibiting migration and invasion through modulating Akt.

BACKGROUND: Huntingtin-interacting protein 1-related (HIP1R) is a multi-domain gene that exerts many cellular functions including altering T cell-mediated cytotoxicity and controlling intracellular trafficking. However, its clinical significance and function in gastric cancer (GC) have not been described. METHODS: The expression levels of HIP1R were tested by the transcriptional and translational expression analysis and immunohistochemistry (IHC) in matched adjacent non-tumorous vs tumor tissue specimens. The biological function of HIP1R on apoptosis, migration, and proliferation was evaluated by flow cytometry, Transwell, Cell Counting Kit-8 (CCK-8) assays, colony formation assays, and EdU labeling assays, respectively. RESULTS: We found downregulated HIP1R in GC compared with adjacent non-tumorous tissue, and HIP1R expression associated with N classification. We further found that the expression of HIP1R could induce apoptosis and inhibit proliferation, migration, invasion of GC cells, possibly through modulating Akt. CONCLUSIONS: Our data indicate that HIP1R may act as a potential diagnostic biomarker and a tumor suppressor gene in GC, potentially representing a novel therapeutic target for future GC treatment.

Designed Fabrication of Polymer-Mediated MOF-Derived Magnetic Hollow Carbon Nanocages for Specific Isolation of Bovine Hemoglobin.

It is highly required to develop well-designed separation materials for the specific isolation of certain proteins in proteomic research. Herein, the new type of metal-organic framework (MOF)-derived polymer-mediated magnetic hollow nanocages was fabricated via stress-induced orientation contraction, which was further applied for specific enrichment of proteins. The core-shell nanocomposites comprised of polymer-mediated ZIF-67 cores and polydopamine (PDA) shells, after annealing, generated magnetic hollow carbon nanocages with hierarchical pores and structures. Particularly, the magnetic carbonized PDA@F127/ZIF-67 hollow nanocages exhibited a remarkable adsorption capacity toward bovine hemoglobin (BHB) up to 834.3 mg g-1, which was significantly greater than that of the directed carbonized ZIF-67 nanoparticles. The results also exhibited the notable specificity of the obtained nanocages on complex biosamples, including intact mixed proteins and fetal calf serum. The hierarchically hollow porous structure greatly improves the specific surface area and reduces the mass transfer resistance, leading to enhanced high adsorption for target protein BHB. This novel method will be promising for the applications in purification and enrichment of biomacromolecules for complex biosamples, which successfully solve the problem of low adsorption efficiency and tedious separating process of the previous MOF-derived materials.

Selective separation of bovine hemoglobin using magnetic mesoporous rare-earth silicate microspheres.

Selective separation of heme proteins and peptides from complicated biological samples before comprehensive identification and characterization of intact biomolecules which are low stoichiometry is indispensable for ongoing proteomics. Here, three magnetic mesoporous rare-earth silicate (rare-earth = Er, Tm, Yb) microspheres prepared by facile solvothermal method were used as novel adsorbents for the selective isolation of bovine hemoglobin (BHb), and magnetic mesoporous ytterbium silicate showed apparent adsorption efficiency in isolating BHb with a adsorption capacity of 304.4 mg/g after the comparison. The retained BHb could be eluted by using Na2CO3 as stripping reagent. Meanwhile, Circular dichroism spectra illustrated that the microspheres posed no effect on the secondary structure of BHb. The resultant magnetic particles were characterized by transmission electron microscope, scanning electron microscope, x-ray powder diffraction, vibrating sample magnetometer, Fourier transform infrared spectroscopy, N2 adsorption-desorption isotherm and zeta potential. The as-prepared magnetic microspheres showed high specificity for the separation of BHb from bovine plasma as corroborated by SDS-PAGE and MALDI-TOF MS analysis, which would be expected to possess potential application in isolation of other heme proteins in complex biological samples.

Fabrication of Yb3+-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum.

In this work, Yb3+-immobilized hydrophilic phytic-acid-coated magnetic nanocomposites were prepared through a facile route and used to selectively separatrf bovine hemoglobin. Hydrophilic phytic acid (PA) was coated onto the magnetic Fe3O4-PEI via electrostatic interactions, followed by finally chelating with Yb3+ ions, which could produce specific protein binding sites at room temperature in water, and complex instrumentation was not necessary. The performance of as-prepared hybrids (Fe3O4-PEI-PA-Yb3+) was assessed by selectively isolating bovine hemoglobin (BHb). The obtained maximum binding capacity was 347.3 mg g-1. The retained BHb could be eluted under simple elution via using 0.1 M of Na2CO3, giving a recovery of 83%. Moreover, the generation of nanocomposites was demonstrated. In addition, the PA and PEI could improve the hydrophilicity of nanoparticles and further reduce the nonspecific adsorption. Therefore, such nanocomposites were successfully employed to selectively bind and separate BHb from bovine serum as verified by SDS-PAGE and MALDI-TOF MS analysis, providing a new perspective for the isolation of heme proteins in proteomics.

Detection of volatile organic compounds released by wood furniture based on a cataluminescence test system.

Wood furniture is an important source of indoor air pollution. To date, the detection of harmful substances in wood furniture has relied on the control of a single formaldehyde component, therefore the detection and evaluation of pollutants released by wood furniture are necessary. A novel method based on a cataluminescence (CTL) sensor system generated on the surface of nano-3TiO2-2BiVO4 was proposed for the simultaneous detection of pollutants released by wood furniture. Formaldehyde and benzene were selected as a model to investigate the CTL-sensing properties of the sensor system. Field emission scanning electronic microscopy (FESEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were employed to characterize the as-prepared samples. The results showed that the as-prepared test system exhibited outstanding CTL properties such as stable intensity, a high signal-to-noise ratio, and short response and recovery times. In addition, the limit of detection for formaldehyde and benzene was below the standard permitted concentrations. Moreover, the sensor system showed outstanding selectivity for formaldehyde and benzene compared with eight other common volatile organic compounds (VOCs). The performance of the sensor system will enable furniture VOC limit emissions standards to be promulgated as soon as possible.