Subject(s)
Chickens/genetics , Chromosomes/genetics , In Situ Hybridization, Fluorescence , Proteins/genetics , Sequence Homology, Nucleic Acid , Animals , Calcium-Binding Proteins , Chromosomes, Human, Pair 20/genetics , Humans , Intercellular Signaling Peptides and Proteins , Jagged-1 Protein , Membrane Proteins , Physical Chromosome Mapping , Serrate-Jagged ProteinsABSTRACT
We report the cloning of a cDNA encoding human syntaxin 8 (STX8), using the regulator (R) domain of the cystic fibrosis transmembrane conductance regulator (CFTR) as a bait to screen a human fetal lung cDNA library by the yeast two-hybrid system. This gene was found broadly transcribed and its mRNA size is about 1.3 kb. The STX8 gene maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein. Syntaxin 8 contains in its C-terminal half a coiled-coil domain found highly conserved in the t-SNARE (SNAP receptor on target membrane) superfamily of proteins, which are involved in vesicular trafficking and docking. In syntaxin 8, a C-terminal hydrophobic domain may constitute a transmembrane anchor. It was recently shown that CFTR-mediated chloride currents can be regulated by syntaxin 1A, a t-SNARE family member, through direct protein-protein interaction. This raises the possibility that syntaxin 8 may also be involved in such regulations.
Subject(s)
Chromosomes, Human, Pair 17/genetics , Gene Expression , Membrane Proteins/genetics , Vesicular Transport Proteins , Amino Acid Sequence , Animals , Base Sequence , Blotting, Northern , Chromosome Mapping , Cloning, Molecular , Conserved Sequence , Cystic Fibrosis Transmembrane Conductance Regulator/genetics , Cystic Fibrosis Transmembrane Conductance Regulator/metabolism , Expressed Sequence Tags , Humans , Lung/embryology , Lung/metabolism , Membrane Proteins/chemistry , Membrane Proteins/metabolism , Molecular Sequence Data , Open Reading Frames/genetics , Protein Binding , Protein Structure, Secondary , Qa-SNARE Proteins , RNA, Messenger/analysis , RNA, Messenger/genetics , SNARE Proteins , Sequence Homology, Amino Acid , Syntaxin 1 , Yeasts/genetics , Yeasts/metabolismABSTRACT
The realization of physical and genetic maps of the chicken genome is dependent on progress in cytogenetic knowledge of its karyotype. To help achieve this goal, we constructed amplified representative DNA samples of the chicken chromosomes 1, 2, 3, 4, 5, 6, 7, 8, Z, W and of the terminal heterochromatin part of Zq by chromosome microdissection and DOP-PCR amplification. These chromosome DNA samples, which represent about 75% of the chicken genome, were used to generate whole chromosome painting probes for FISH. The direct application of these chromosome specific probes is dual FISH localization and characterization of panels of chicken interspecific somatic hybrids. We discuss some aspects of the chicken genome and its repeated sequences.