ABSTRACT
Hexose transporters (HXT) play a crucial role in the pathogenicity of Magnaporthe oryzae, serving not only as key facilitators for acquiring and transporting sugar nutrients to support pathogen development, but also as sugar sensors which receive transduction signals. The objective of this study is to investigate the impact of MoHXT1-3 on rice pathogenicity and hexose affinity. MoHXT1-3 deletion mutants were generated using CRISPR/Cas9 technology, and their affinity for hexose was evaluated through yeast complementation assays and electrophysiological experiments in Xenopus oocytes. The results suggest that MoHXT1 does not contribute to melanin formation or hexose transportation processes. Conversely, MoHXT2, despite displaying lower affinity towards the hexoses tested in comparison to MoHXT3, is likely to have a more substantial impact on pathogenicity. The analysis of the transcription profiles demonstrated that the deletion of MoHXT2 caused a decrease in the expression of MoHXT3, whereas the knockout of MoHXT3 resulted in an upregulation of MoHXT2 transcription. It is noteworthy that the MoHXT2M145K variant displayed an incapacity to transport hexoses. This investigation into the functional differences in hexose transporters in Magnaporthe oryzae provides insights into potential advances in new strategies to target hexose transporters to combat rice blast by blocking carbon nutrient supply.
ABSTRACT
The integration of advanced functions and diverse practical applications calls for multifunctional liquid crystal elastomers (LCEs); however, the structure-intrinsic luminescence and excellent mechanical properties of LCEs have not yet been explored. In this study, clusteroluminescence (CL)-based LCEs (CL-LCEs) are successfully fabricated without depending on large conjugated structures, thereby avoiding redundant organic synthesis and aggregation-caused quenching. The experimental and theoretical results reveal that secondary amine (-NH-) and imine (-C = N-) groups play vital roles in determining the presence of fluorescence in CL-LCEs. Based on the above observation, the strategy universalization and a molecular library for constructing CL-LCEs are further demonstrated. Meanwhile, the dynamic bond of imine bonds endows the CL-LCE system with rapid self-healing under mild conditions (70 °C in 10 min), excellent stretchability, and adaptive programmable characteristics. Furthermore, the self-luminescent performance enables visual detection of the self-healing process. Finally, CL-based information storage and anticounterfeiting are successfully realized and their applications in fiber actuators and fluorescent textiles are demonstrated. The distinctive luminescence and dynamic chemistry presented in this work has significant implications in elucidating the mechanism of CL and providing new strategies for the rational design of novel multifunctional LCE materials.
ABSTRACT
A smart window is an optical dimming device with intelligent functions that can control its relevant performances through external stimuli, achieving functions such as privacy protection and temperature regulation. Light is an ideal stimulus for regulating smart windows, which is noninvasive and allows self-adaptable manipulation of materials. This review highlights recent significant achievements in smart windows constructed by photo-responsive liquid crystals (LCs) systems that can undergo the transition between different phases. The smart windows based on photo-responsive LCs are used in a plethora of areas, including privacy protection, absorption glass, building decoration, energy saving, and climate modulation applications. The review concludes with a brief perspective on some significant challenges and opportunities for the future development of photo-responsive smart windows, which is crucial for expanding the applications of smart windows and improving their performances.
ABSTRACT
A polymer-stabilized liquid crystal (PSLC)-based environment-adaptative smart window with multi-modulations is demonstrated. This PSLC system contains a right-handed dithienyldicyanoethene-based chiral photoswitch and a chiral dopant, S811, with opposite handedness, of which the reversible cis-trans photoisomerization of the switch can drive self-shading of the smart window under UV light stimulus because of the transition from nematic phase to cholesteric one. With the assistance of solar heat, the opacity of the smart window can be deepened because the heat promotes the isomerization conversion rate of the switch. This switch has no thermal relaxation at room temperature, therefore, the smart window exhibits dual stabilization: transparent state (cis-isomer) and opaque state (trans-isomer). Moreover, the incident intensity of sunlight can be regulated by an electric field, which allows the smart window to adapt to some specific situations. Such an energy-saving device can be used in buildings and vehicles to control indoor temperature and adapt to the required ambiance.
ABSTRACT
Developing circularly polarized room-temperature phosphorescent (CPRTP) materials with a high dissymmetry factor (glum) and long afterglow is very attractive but highly challenging. Here, a CPRTP emission featuring ultrahigh glum value and desired visualization characteristic in a bilayer composite photonic film is achieved for the first time. In the constructed system, N and P co-doped carbonized polymer dots (NP-CPDs) are dispersed into polyvinyl alcohol (PVA) as the phosphorescent emitting layer, and helically structured cholesteric polymer films are used as selective reflective layers to convert the unpolarized emission of NP-CPDs into circularly polarized emission. On the basis of the modulation of the helical structure period of the cholesteric polymer, the bilayer composite film enables NP-CPDs to obtain a high glum value. Notably, the optimized photonic film emits CPRTP with glum as high as 1.09 and a green afterglow lasting above 8.0 s. Moreover, the composite photonic array films featuring information encryption characteristics are developed by modulating the liquid crystal phase of the cholesteric polymer film and the dot coating position of the NP-CPDs/PVA layer, thus expanding the application of CPRTP materials in cryptography and anti-counterfeiting.