ABSTRACT
Particle manipulation through the transfer of light or sound momentum has emerged as a powerful technique with immense potential in various fields, including cell biology, microparticle assembly, and lab-on-chip technology. Here, we present a novel method called Programmable Photoacoustic Manipulation (PPAM) of microparticles in liquid, which enables rapid and precise arrangement and controllable transport of numerous silica particles in water. Our approach leverages the modulation of pulsed laser using digital micromirror devices (DMD) to generate localized Lamb waves in a stainless steel membrane and acoustic waves in water. The particles undergo a mechanical force of about several µN due to membrane vibrations and an acoustic radiation force of about tens of nN from the surrounding water. Consequently, this approach surpasses the efficiency of optical tweezers by effectively countering the viscous drag imposed by water and can be used to move thousands of particles on the membrane. The high power of the pulsed laser and the programmability of the DMD enhance the flexibility in particle manipulation. By integrating the benefits of optical and acoustic manipulation, this technique holds great promise for advancing large-scale manipulation, cell assembly, and drug delivery.
ABSTRACT
The advancement of optoelectronic applications relies heavily on the development of high-performance photodetectors that are self-driven and capable of detecting a wide range of wavelengths. CsPbI3 nanorods (NRs), known for their outstanding optical and electrical properties, offer direct bandgap characteristics, high absorption coefficients, and long carrier diffusion lengths. However, challenges such as stability and limited photoluminescence quantum yield have impeded their widespread application. By integrating PbSe colloidal quantum dots (CQDs) with CsPbI3 NRs, the hybrid nanomaterial harnesses the benefits of each component, resulting in enhanced optoelectronic properties and device performance. In this work, a self-powered and broadband photodetector, ITO/ZnO/CsPbI3:PbSe/CuSCN/Au, is fabricated, in which CsPbI3 NRs are decorated with PbSe QDs as the photoactive layer, ZnO as the electron-transporting layer and CuSCN as the hole-transporting layer. The device performance is further improved through the incorporation of Cs2CO3 into the ZnO layer, resulting in an enhancement of its overall operational characteristics. As a result, a notable responsivity of 9.29 A W-1 and a specific detectivity of 3.17 × 1014 Jones were achieved. Certainly, the TCAD simulations closely correlate with our experimental data, facilitating a comprehensive exploration of the fundamental physical mechanisms responsible for the improved performance of these surface-passivated heterojunction photodetectors. This opens up exciting possibilities for substantial advancements in the realm of next-generation optoelectronic devices.
ABSTRACT
In the fields of biomedicine and microfluidics, the non-contact capture, manipulation, and spin of micro-particles hold great importance. In this study, we propose a programmable non-contact manipulation technique that utilizes photoacoustic effect to spin and transport living shrimp eggs. By directing a modulated pulsed laser toward a liquid-covered stainless-steel membrane, we can excite patterned Lamb waves within the membrane. These Lamb waves occur at the interface between the membrane and the liquid, enabling the manipulation of nearby particles. Experimental results demonstrate the successful capture, spin, and transport of shrimp eggs in diameter of 220â µm over a distance of about 5â mm. Calculations indicate that the acoustic radiation force and torque generated by our photoacoustic manipulation system are more than 299.5â nN and 41.0â nN·mm, respectively. The system surpasses traditional optical tweezers in terms of force and traditional acoustic tweezers in terms of flexibility. Consequently, this non-contact manipulation system significantly expands the possibilities for applications in various fields, including embryo screening, cell manipulation, and microfluidics.
Subject(s)
Ovum , Photoacoustic Techniques , Animals , Photoacoustic Techniques/methods , Pressure , Optical Tweezers , PenaeidaeABSTRACT
Optical and acoustic tweezers, despite operating on different physical principles, offer non-contact manipulation of microscopic and mesoscopic objects, making them essential in fields like cell biology, medicine, and nanotechnology. The advantages and limitations of optical and acoustic manipulation complement each other, particularly in terms of trapping size, force intensity, and flexibility. We use photoacoustic effects to generate localized Lamb wave fields capable of mapping arbitrary laser pattern shapes. By using localized Lamb waves to vibrate the surface of the multilayer membrane, we can pattern tens of thousands of microscopic particles into the desired pattern simultaneously. Moreover, by quickly and successively adjusting the laser shape, microparticles flow dynamically along the corresponding elastic wave fields, creating a frame-by-frame animation. Our approach merges the programmable adaptability of optical tweezers with the potent manipulation capabilities of acoustic waves, paving the way for wave-based manipulation techniques, such as microparticle assembly, biological synthesis, and microsystems.
ABSTRACT
Due to the characteristics of ultra-short pulse width and ultra-high peak power, femtosecond pulse laser can effectively induce nonlinear optical effects in trapped objects. As a result, it holds great value in the fields of micro and nano manipulation, microfluidics, and cell biology. However, the nonlinear optical effects on the stiffness of femtosecond optical traps remain unclear. Calibration of trap stiffness is crucial for accurately measuring forces and manipulating small particles. In this paper, we compare the stiffness between femtosecond optical traps and continuous wave optical traps. Experimental results demonstrate that the stiffness of the femtosecond optical trap in the splitting direction is greater than that in other directions and the stiffness of the continuous wave optical trap under the same laser power condition. Additionally, as the laser power increases, the stiffnesses of both the femtosecond optical trap and the continuous wave optical trap gradually increases. In contrast to a linear increase of the continuous wave optical trap, the stiffness of the femtosecond optical trap exhibits an exponential rise with increasing laser power. This research provides guidance and reference for improving the force measurement accuracy of femtosecond optical tweezer system.
ABSTRACT
Locoregional therapy has attracted increasing attention for subcutaneous tumors owing to its merits of minimally invasive operation and negligible systematic toxicity. However, to accelerate clinical translation, further promotions in deep-seated penetration, temporal-spatial controllability, personalized adaptability, as well as easy operation are still urgently needed. This work proposed a self-heating-cooking-package-inspired hydrothermally responsive multi-round acturable microneedle (HRMAM) system, which loaded docetaxel (DTX) in the polycaprolactone (PCL), to serve as deeply penetrable, hydrothermal-chemotherapy synergetic, on-demand and self-service anti-tumor treatment. The optimized hydrothermally responsive formulation served as base components for water-based self-heating responsive drug release with synergistic anti-tumor thermal therapy, and simultaneously in combination with well-designed grooved-structure needle tips for directional deep delivery and enhanced transfer efficiency. To satisfy spatial precision and flexible controllability, this engineering-based detachable HRMAM system was divided into three relatively independent segments, which were fitted perfectly with an original-designed applicator for ensuring easy operation in a smart self-service manner. Impressively, the HRMAM system achieved encouraging tumor growth inhibition of 75.11% and 72.29% in animal model of melanomas and breast carcinoma, respectively, much higher than those of other groups receiving traditional treatment, without obvious side effects. It was anticipated that the HRMAM system would manifest great promise to combat unreachable and deep-seated subcutaneous tumors, bellowing clinical values upon locoregional therapy products with high efficiency, low toxicity, flexible controllability, temporal-spatial precision, easy operation, as well as patient's painless, comfort and compliance.
Subject(s)
Antineoplastic Agents , Neoplasms , Animals , Neoplasms/drug therapy , Docetaxel/therapeutic use , Micelles , Drug Liberation , Cell Line, Tumor , Drug Delivery Systems , Antineoplastic Agents/therapeutic useABSTRACT
This research investigated the mechanism of ozone treatment on sweet cherry (Prunus avium L.) by Lable-free quantification proteomics and physiological traits. The results showed that 4557 master proteins were identified in all the samples, and 3149 proteins were common to all groups. Mfuzz analyses revealed 3149 candidate proteins. KEGG annotation and enrichment analysis showed proteins related to carbohydrate and energy metabolism, protein, amino acids, and nucleotide sugar biosynthesis and degradation, and fruit parameters were characterized and quantified. The conclusions were supported by the fact that the qRT-PCR results agreed with the proteomics results. For the first time, this study reveals the mechanism of cherry in response to ozone treatment at a proteome level.
ABSTRACT
Among 2D materials (Xenes) which are at the forefront of research activities, borophene, is an exciting new entry due to its uniquely varied optical, electronic, and chemical properties in many polymorphic forms with widely varying band gaps including the lightest 2D metallic phase. In this paper, we used a simple selective chemical etching to prepare borophene with a strong near IR light-induced photothermal effect. The photothermal efficiency is similar to plasmonic Au nanoparticles, with the added benefit of borophene being degradable due to electron deficiency of boron. We introduce this selective chemical etching process to obtain ultrathin and large borophene nanosheets (thickness of ~4 nm and lateral size up to ~600 nm) from the precursor of AlB2. We also report first-time observation of a selective Acid etching behavior showing HCl etching of Al to form a residual B lattice, while HF selectively etches B to yield an Al lattice. We demonstrate that through surface modification with polydopamine (PDA), a biocompatible smart delivery nanoplatform of B@PDA can respond to a tumor environment, exhibiting an enhanced cellular uptake efficiency. We demonstrate that borophene can be more suitable for safe photothermal theranostic of thick tumor using deep penetrating near IR light compared to gold nanoparticles which are not degradable, thus posing long-term toxicity concerns. With about 40 kinds of borides, we hope that our work will open door to more discoveries of this top-down selective etching approach for generating borophene structures with rich unexplored thermal, electronic, and optical properties for many other technological applications.
ABSTRACT
Circular dichroism (CD), as one of the most representative chiroptical effects, provides a simple strategy for the detection and characterization of the molecular chirality. The enhancement and sign reversal of CD are of great importance for its practical applications in chiral bio-sensing, chirality switching and optical filtering, etc. Here, we realize considerable adjustments and the sign reversal of CD in quasi-three-dimensional (quasi-3D) combined Archimedean spiral nanostructures. With special local and lattice configurations, the nanostructures have both right-handed and left-handed geometric chirality, which are designed based on the proximity effect of stencil lithography. We find that the CD response of the nanostructures becomes obvious once its height exceeds 200â nm and can be adjusted by the further increase of the height or the change of the blade spacing of the nanostructures. The CD reversal is achieved by utilizing the competition of two chiral centers when the height or blade spacing exceeds a critical value. Further analysis of the scattering power of multipole moments reveals that the CD modulation is determined by both magnetic dipole moment and electric quadrupole moment. Benefiting from the highly sensitive CD response to the height, the extreme sign reversal of CD is achieved when a sub-10-nm ultrathin medium layer is anchored on the surface of the nanostructures, which provides a promising strategy for ultra-sensitive chiral bio-sensing.
ABSTRACT
The ability of light beams to rotate nano-objects has important applications in optical micromachines and biotechnology. However, due to the diffraction limit, it is challenging to rotate nanoparticles at subwavelength scale. Here, we propose a method to obtain controlled fast orbital rotation (i.e., circumgyration) at deep subwavelength scale, based on the nonlinear optical effect rather than sub-diffraction focusing. We experimentally demonstrate rotation of metallic nanoparticles with orbital radius of 71 nm, to our knowledge, the smallest orbital radius obtained by optical trapping thus far. The circumgyration frequency of particles in water can be more than 1 kHz. In addition, we use a femtosecond pulsed Gaussian beam rather than vortex beams in the experiment. Our study provides paradigms for nanoparticle manipulation beyond the diffraction limit, which will not only push toward possible applications in optically driven nanomachines, but also spur more fascinating research in nano-rheology, micro-fluid mechanics and biological applications at the nanoscale.
ABSTRACT
All-optical photonic crystal diodes based on the Fano resonance of nonlinear defects are studied. The diodes can achieve nonreciprocal transmission ratios of 31.7 dB and 33.9 dB at working wavelengths of 1534.83 nm and 1536.02 nm, respectively. The function of two defects' coupling to the performance of unidirectional light transmission is also analyzed. When two Fano cavities are cascaded to form a two-branch-channel diode, unidirectional light propagation at 1536.88, 1538.76, 1612.80, and 1616.78 nm wavelengths is achieved along two opposite forward directions, and the nonreciprocal transmission ratios are 36.5, 30.3, 23.9, and 19.6 dB, respectively.
ABSTRACT
Based on the nonlinear resonators and self-collimation characteristics of light beams, we designed an all-optical photonic crystal beam splitter and switch. The proposed device consists of an input waveguide and three output waveguides connected to different ring resonators. Three pump beams transmit through different resonators via the self-collimation effect, and eight output states are realized by altering the intensity of the pump light. The proposed device works at the wavelength of 1629.57 nm, and the pump wavelength is located at 1240.00 nm. The transmittance contrast between the "on" and "off" states reached a maximum value of 124.0 and a minimum of 17.6. The minimal pump light intensity required to implement the performance is only ${0.162}\,\,{\rm W/}\unicode{x00B5}{\rm m}^2$0.162W/µm2, while the maximal value is about ${0.497}\,\,{\rm W/}\unicode{x00B5}{\rm m}^2$0.497W/µm2. Due to the small size of our proposed device and also its insensitivity to the pump light beams' incident location and spatial width within a certain degree, it has great potential application value in all-optical communications.
ABSTRACT
Ultrasound fields have broad applications in imaging, sensing, medical therapy, etc. In these applications, it is of great importance to generate desired ultrasound fields. The generation of arbitrary ultrasound fields is challenging using phased array transducers or a monolithic acoustic hologram. In this work, by taking advantage of the photoacoustic effect and spatial light modulating technique, we demonstrate that dynamic and high-resolution arbitrary acoustic fields in liquid can be realized. We clearly show ultrasonic vortex and arbitrary 2D/3D ultrasonic fields in our photoacoustic system. All the measured pressure fields agree well with the desired ones. We anticipate these rapidly tunable photoacoustic fields will find applications in dynamic acoustic tweezers and ultrasonic imaging.
ABSTRACT
The sensitivities of the octagonal quasi-periodic photonic crystal (QPC) defective modes are theoretically studied. The octagonal QPC biosensors are composed of silicon columns arranged in a liquid background. By designing a defect structure, a variety of localized modes with different spatial symmetries and field profiles are obtained, and a maximum refractive index sensitivity 800 nm/RIU is achieved around 1500 nm transmission peak when the central rod's size equals 100 nm, and the corresponding detection limit reaches 0.00042. The liquid can flow freely among the rods through the entire structure, so it is convenient to monitor the concentration of protein in the liquid environment dynamically. The influence of the protein's thickness to the shift of the resonant wavelength is also studied, where a minimum protein's thickness of less than 10 nm can be detected by optimizing the central column's size to be 400 nm, and the spatial field profiles of different resonant modes are analyzed to explain the corresponding sensitivities.
Subject(s)
Biosensing Techniques/instrumentation , Nanostructures/chemistry , Silicon/chemistry , Limit of Detection , Photons , Sensitivity and SpecificityABSTRACT
BACKGROUND: The fermentation performance of a genome-shuffled strain of Candida versatilis S3-5, isolated for improved tolerance to salt, and wild-type (WT) strain were analysed. The fermentation parameters, such as growth, reducing sugar, ethanol, organic acids and volatile compounds, were detected during soy sauce fermentation process. RESULTS: The results showed that ethanol produced by the genome shuffled strain S3-5 was increasing at a faster rate and to a greater extent than WT. At the end of the fermentation, malic acid, citric acid and succinic acid formed in tricarboxylic acid cycle after S3-5 treatment elevated by 39.20%, 6.85% and 17.09% compared to WT, respectively. Moreover, flavour compounds such as phenethyl acetate, ethyl vanillate, ethyl acetate, isoamyl acetate, ethyl myristate, ethyl pentadecanoate, ethyl palmitate and phenylacetaldehyde produced by S3-5 were 2.26, 2.12, 2.87, 34.41, 6.32, 13.64, 2.23 and 78.85 times as compared to WT. CONCLUSIONS: S3-5 exhibited enhanced metabolic ability as compared to the wild-type strain, improved conversion of sugars to ethanol, metabolism of organic acid and formation of volatile compounds, especially esters, Moreover, S3-5 might be an ester-flavour type salt-tolerant yeast. © 2016 Society of Chemical Industry.
Subject(s)
Candida/genetics , Candida/metabolism , Fermentation/genetics , Food Handling/methods , Genetic Engineering , Salt Tolerance/genetics , Candida/growth & development , Carbohydrate Metabolism , Citric Acid/metabolism , Ethanol/metabolism , Flavoring Agents , Genome, Fungal/genetics , Glucosamine/analogs & derivatives , Glucosamine/metabolism , Malates/metabolism , Soy Foods/microbiology , Succinic Acid/metabolism , Taste , Volatile Organic Compounds/metabolismABSTRACT
Controlled propulsion of microparticles and micromachines in fluids could revolutionize many aspects of technology, such as biomedicine, microfluidics, micro-mechanics, optomechanics, and cell biology. We report the self-propelled cyclic round-trip motion of metallo-dielectric Janus particles in static line optical tweezers (LOT). The Janus particle is a 5 µm-diameter polystyrene sphere half-coated with 3 nanometer thick gold film. Both experiment and theory show that this cyclic translational and rotational motion is a consequence of the collective and fine action of the gold-face orientation dependent propulsion optical force, the gradient optical force, and the spontaneous symmetry breaking induced optical torque in different regions of the LOT. This study indicates a novel way to propel and manipulate the mechanical motion of microscopic motors and machines wirelessly in fluid, air, or vacuum environments using a static optical field with a smartly designed non-uniform intensity profile allowing fully controlled momentum and angular momentum exchange between light and the particle.
ABSTRACT
Bistable rotation is realized for a gold-coated Janus colloidal particle in an infrared optical trap. The metal coating on the Janus particles are patterned by sputtering gold on a monolayer of closely packed polystyrene particles. The Janus particle is observed to stably rotate in an optical trap. Both the direction and the rate of rotation can be experimentally controlled. Numerical calculations reveal that the bistable rotation is the result of spontaneous symmetry breaking induced by the uneven curvature of the coating patterns on the Janus sphere. Our results thus provide a simple method to construct large quantities of fully functional rotary motors for nano- or microdevices.
ABSTRACT
Bacillus coagulans 2-6 is an excellent producer of optically pure l-lactic acid. However, little is known about the mechanism of synthesis of the highly optically pure l-lactic acid produced by this strain. Three enzymes responsible for lactic acid production-NAD-dependent l-lactate dehydrogenase (l-nLDH; encoded by ldhL), NAD-dependent d-lactate dehydrogenase (d-nLDH; encoded by ldhD), and glycolate oxidase (GOX)-were systematically investigated in order to study the relationship between these enzymes and the optical purity of lactic acid. Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 (a d-lactic acid producer) and Lactobacillus plantarum subsp. plantarum DSM 20174 (a dl-lactic acid producer) were also examined in this study as comparative strains, in addition to B. coagulans. The specific activities of key enzymes for lactic acid production in the three strains were characterized in vivo and in vitro, and the levels of transcription of the ldhL, ldhD, and GOX genes during fermentation were also analyzed. The catalytic activities of l-nLDH and d-nLDH were different in l-, d-, and dl-lactic acid producers. Only l-nLDH activity was detected in B. coagulans 2-6 under native conditions, and the level of transcription of ldhL in B. coagulans 2-6 was much higher than that of ldhD or the GOX gene at all growth phases. However, for the two Lactobacillus strains used in this study, ldhD transcription levels were higher than those of ldhL. The high catalytic efficiency of l-nLDH toward pyruvate and the high transcription ratios of ldhL to ldhD and ldhL to the GOX gene provide the key explanations for the high optical purity of l-lactic acid produced by B. coagulans 2-6.
Subject(s)
Bacillus/enzymology , Bacillus/metabolism , Lactate Dehydrogenases/metabolism , Lactic Acid/metabolism , Bacillus/genetics , Fermentation , Gene Expression Profiling , Kinetics , Lactate Dehydrogenases/genetics , Lactobacillus delbrueckii/enzymology , Lactobacillus delbrueckii/genetics , Lactobacillus delbrueckii/metabolism , Lactobacillus plantarum/enzymology , Lactobacillus plantarum/genetics , Lactobacillus plantarum/metabolismABSTRACT
BACKGROUND: This study aimed to enhance and improve the quality and safety of soy sauce. In the present work, the change of biogenic amines, such as histamine, tyramine, cadaverine, spermidine, was examined by the treatment of Candida versatilis and Zygosaccharomyces rouxii, and the influence of salt-tolerant yeast on biogenic amines was analysed during the whole fermentation process. RESULTS: The results showed that the content of biogenic amines was elevated after yeast treatment and the content of biogenic amines was influenced by using yeast. The dominating biogenic amine in soy sauce was tyramine. At the end of fermentation, the concentrations of biogenic amines produced by Zygosaccharomyces rouxii and Candida versatilis in the soy mash were 122.71 mg kg(-1) and 69.96 mg kg(-1) . CONCLUSIONS: The changes of biogenic amines in high-salt liquid soy mash during fermentation process indicated that a variety of biogenic amines were increased in the fermentation ageing period, which may be due to amino acid decarboxylation to form biogenic amines by yeast decarboxylase. The fermentation period of soy sauce should be longer than 5 months because biogenic amines began to decline after this time period.
Subject(s)
Biogenic Amines/biosynthesis , Candida/metabolism , Fermentation , Food Handling/methods , Soy Foods , Zygosaccharomyces/metabolism , Biogenic Amines/analysis , Cadaverine/analysis , Candida/growth & development , Histamine/analysis , Hydrogen-Ion Concentration , Soy Foods/analysis , Soy Foods/microbiology , Spermidine/analysis , Time Factors , Tyramine/analysis , Zygosaccharomyces/growth & developmentABSTRACT
Small interfering RNA (siRNA) has the potential to influence gene expression with a high degree of target gene specificity. However, the clinical application of siRNA therapeutics has proven to be less promising as evidenced by its poor intracellular uptake, instability in vivo, and nonspecific immune stimulations. Recently, we have demonstrated that single-walled carbon nanotube (SWNT)-mediated siRNA delivery can enhance the efficiency of siRNA-mediated gastrin-releasing peptide receptor (GRP-R) gene silencing by stabilizing siRNA while selectively targeting tumor tissues. Based on our recent findings, we introduce a novel technique to silence specific gene(s) in human neuroblastoma through SWNT-mediated siRNA delivery in vitro and in vivo.