Galectin-7 promotes the invasiveness of human oral squamous cell carcinoma cells via activation of ERK and JNK signaling.

Galectin-7 is a member of the ß-galactoside-binding protein family, and is highly expressed in oral squamous cell carcinoma (OSCC). The aim of the present study was to investigate the effects of manipulating galectin-7 expression on the biological phenotype of human OSCC cells and the associated molecular mechanisms. Knockdown of endogenous galectin-7 via small interfering RNA (siRNA) was performed and cell proliferation, apoptosis, migration, and invasion were subsequently assessed. The data indicated that galectin-7 silencing had no impact on the proliferation or apoptosis of OSCC cells. However, compared with non-transfected cells, percentage wound closure was significantly lower in galectin-7-silenced cells following 24 h incubation, indicating decreased cell migration. Furthermore, Matrigel invasion assays demonstrated that galectin-7 knockdown significantly reduced the number of invaded cells, compared with the number in non-transfected cells. Western blot analysis indicated that galectin-7 overexpression resulted in a significant increase in the expression of the proteins matrix metalloproteinase (MMP)-2 and MMP-9. The invasive abilities of cells overexpressing galectin-7 significantly decreased following co-transfection with MMP-2- or MMP-9-specific siRNA. Increasing galectin-7 expression significantly enhanced the phosphorylation of extracellular signal-related kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK) 1/2. Pharmacological inhibition of ERK or JNK activity significantly suppressed the invasiveness of galectin-7-overexpressing cells and abrogated the upregulation of MMP-2 and MMP-9. Taken together, the results of the current study provide novel evidence for the pro-invasive activity of galectin-7 in OSCC cells, which is associated with activation of ERK and JNK signaling and the induction of MMP-2 and MMP-9.

[Analysis of effect of military outpatients' psychological feelings on doctor-patient relationship in orthodontic treatment].

PURPOSE: To explore the effect of military outpatients' psychological feelings on doctor-patient relationship in orthodontic treatment. METHODS: A total of 95 clinic patients who were undergoing orthodontic treatment were asked to complete the questionnaire of dental visit satisfaction scale (DVSS) and orthodontist-patient relationship scale (OPRS). The results of different groups according to identity, gender, age and treatment time were compared. The data was analyzed with SPSS 20.0 software package for correlation analysis and stepwise regression. RESULTS: Except 6-18 months group, psychological feelings of patients had significant impact on doctor-patient relationship (P<0.01). The correlation coefficient was 0.610 between patients' satisfaction of outpatient treatment and doctor-patient relationship (P<0.001). Among 9 psychological feelings of orthodontic treatment, five kinds had significant effect on doctor-patient relationship, including three kinds of positive correlation and two kinds of negative correlation. CONCLUSIONS: The patients' psychological feelings are closely related to doctor-patient relationship. Doctors should try to improve medical quality, strengthen service level and pay much attention to the patients' psychological and social needs.

Prefabrication of axial vascularized tissue engineering coral bone by an arteriovenous loop: a better model.

The most important problem for the survival of thick 3-dimensional tissues is the lack of vascularization in the context of bone tissue engineering. In this study, a modified arteriovenous loop (AVL) was developed to prefabricate an axial vascularized tissue engineering coral bone in rabbit, with comparison of the arteriovenous bundle (AVB) model. An arteriovenous fistula between rabbit femoral artery and vein was anastomosed to form an AVL. It was placed in a circular side groove of the coral block. The complex was wrapped with an expanded-polytetrafluoroethylene membrane and implanted beneath inguinal skin. After 2, 4, 6 and 8 weeks, the degree of vascularization was evaluated by India ink perfusion, histological examination, vascular casts, and scanning electron microscopy images of vascular endangium. Newly formed fibrous tissues and vasculature extended over the surfaces and invaded the interspaces of entire coral block. The new blood vessels robustly sprouted from the AVL. Those invaginated cavities in the vascular endangium from scanning electron microscopy indicated vessel's sprouted pores. Above indexes in AVL model are all superior to that in AVB model, indicating that the modified AVL model could more effectively develop vascularization in larger tissue engineering bone.

[Incomplete palate cleft patient with mirror-image dextrocardia and situs inversus viscerums: report of one case].

Cleft palate is one of the most common congenital defects, occurring alone or in combination with other malformations. Cleft palate with mirror-image dextrocardia and situs inversus viscerums is rare. This paper presented a case of incomplete cleft palate patient with mirror-image dextrocardia and situs inversus viscerums. The patient had a family history of cleft palate and had no family history of congenital heart disease. He was found to have congenital heart disease during preoperative assessment. Without intracardiac malformation, the patient was routinely operated under general anesthesia. The patient had an uneventful postoperative course and was discharged 8 days after operation. Further investigation is needed whether multiple defects in this patient are independent diseases or a part of possible recessive syndrome.