ABSTRACT
The development of artificial intelligence-enabled medical health care has created both opportunities and challenges for next-generation biosensor technology. Proteins are extensively used as biological macromolecular markers in disease diagnosis and the analysis of therapeutic effects. Electrochemical protein biosensors have achieved desirable specificity by using the specific antibody-antigen binding principle in immunology. However, the active centers of protein biomarkers are surrounded by a peptide matrix, which hinders charge transfer and results in insufficient sensor sensitivity. Therefore, electrode-modified materials and transducer devices have been designed to increase the sensitivity and improve the practical application prospects of electrochemical protein sensors. In this review, we summarize recent reports of electrochemical biosensors for protein biomarker detection. We highlight the latest research on electrochemical protein biosensors for the detection of cancer, viral infectious diseases, inflammation, and other diseases. The corresponding sensitive materials, transducer structures, and detection principles associated with such biosensors are also addressed generally. Finally, we present an outlook on the use of electrochemical protein biosensors for disease marker detection for the next few years.
ABSTRACT
The intensity coupling characteristics of Ne dual-isotope inflation and dual-longitudinal-mode operation ring lasers were investigated based on the Lamb theory. Considering the contribution of the Ne isotope system to the polarization of the gain medium and gain saturation effects, the frequency coupling effects were analyzed. Combined with the plasma dispersion function, the optical cavity length is 0.47 m, Ne20: Ne22= 0.53:0.47; the frequency spacing of the adjacent longitudinal mode is 640â MHz, and the intensity tuning curve of the ring laser is simulated. The alterations in the gain self-saturation and mutual saturation coefficients between the four frequencies generated via dual-longitudinal mode splitting are comprehensively discussed. Finally, a detection experiment for the intensity-tuning curve is designed to verify the theoretical analysis.
ABSTRACT
Achieving convenient and accurate detection of indoor ppb-level formaldehyde is an urgent requirement to ensure a healthy working and living environment for people. Herein, ultrasmall In2O3 nanorods and supramolecularly functionalized reduced graphene oxide are selected as hybrid components of visible-light-driven (VLD) heterojunctions to fabricate ppb-level formaldehyde (HCHO) gas sensors (named InAG sensors). Under 405 nm visible light illumination, the sensor exhibits an outstanding response toward ppb-level HCHO at room temperature, including the ultralow practical limit of detection (pLOD) of 5 ppb, high response (Ra/Rg = 2.4, 500 ppb), relatively short response/recovery time (119 s/179 s, 500 ppb), high selectivity, and long-term stability. The ultrasensitive room temperature HCHO-sensing property is derived from visible-light-driven and large-area heterojunctions between ultrasmall In2O3 nanorods and supramolecularly functionalized graphene nanosheets. The performance of the actual detection toward HCHO is evaluated in a 3 m3 test chamber, confirming the practicability and reliability of the InAG sensor. This work provides an effective strategy for the development of low-power-consumption ppb-level gas sensors.
ABSTRACT
The demand for the ubiquitous detection of gases in complex environments is driving the design of highly specific gas sensors for the development of the Internet of Things, such as indoor air quality testing, human exhaled disease detection, monitoring gas emissions, etc. The interaction between analytes and bioreceptors can described as a "lock-and-key", in which the specific catalysis between enzymes and gas molecules provides a new paradigm for the construction of high-sensitivity and -specificity gas sensors. The electrochemical method has been widely used in gas detection and in the design and construction of enzyme-based electrochemical gas sensors, in which the specificity of an enzyme to a substrate is determined by a specific functional domain or recognition interface, which is the active site of the enzyme that can specifically catalyze the gas reaction, and the electrode-solution interface, where the chemical reaction occurs, respectively. As a result, the engineering design of the enzyme electrode interface is crucial in the process of designing and constructing enzyme-based electrochemical gas sensors. In this review, we summarize the design of enzyme-based electrochemical gas sensors. We particularly focus on the main concepts of enzyme electrodes and the selection and design of materials, as well as the immobilization of enzymes and construction methods. Furthermore, we discuss the fundamental factors that affect electron transfer at the enzyme electrode interface for electrochemical gas sensors and the challenges and opportunities related to the design and construction of these sensors.
Subject(s)
Electrochemical Techniques , Gases , Humans , Catalysis , Electrodes , Electron TransportABSTRACT
Formaldehyde (HCHO) sensing plays a critical role for indoor environment monitoring in smart home systems. Inspired by the unique hierarchical structure of cactus, we have prepared a ZnO/ANS-rGO composite for room-temperature (RT) HCHO sensing, through assembling hollow cactus-like ZnO nanorods with 5-aminonaphthalene-1-sulfonic acid (ANS)-modified graphene nanosheets in a facile and template-free manner. Interestingly, it was found that the ZnO morphology could be simply tuned from flower clusters to hollow cactus-like nanostructures, along with the increase of the reaction time during the assembly process. The ZnO/ANS-rGO-based sensors exhibited superior RT HCHO-sensing performance with an ultrahigh response (68%, 5 ppm), good repeatability, long-term stability, and an outstanding practical limit of detection (LOD: 0.25 ppm) toward HCHO, which is the lowest practical LOD reported so far. Furthermore, for the first time, a 30 m3 simulation test cabinet was adapted to evaluate the practical gas-sensing performance in an indoor environment. As a result, an instantaneous response of 5% to 0.4 ppm HCHO was successfully achieved in the simulation test. The corresponding sensing mechanism was interpreted from two aspects including high charge transport capability of ANS-rGO and the distinct gas adsorbability derived from nanostructures, respectively. The combination of a biomimetic hierarchical structure and supramolecular assembly provides a promising strategy to design HCHO-sensing materials with high practicability.
