ABSTRACT
Tubulin, one of the most abundant cytoskeletal building blocks, has numerous isotypes in metazoans encoded by different conserved genes. Whether these distinct isotypes form cell type- and context-specific microtubule structures is poorly understood. Based on a cohort of 12 patients with primary ciliary dyskinesia as well as mouse mutants, we identified and characterized variants in the TUBB4B isotype that specifically perturbed centriole and cilium biogenesis. Distinct TUBB4B variants differentially affected microtubule dynamics and cilia formation in a dominant-negative manner. Structure-function studies revealed that different TUBB4B variants disrupted distinct tubulin interfaces, thereby enabling stratification of patients into three classes of ciliopathic diseases. These findings show that specific tubulin isotypes have distinct and nonredundant subcellular functions and establish a link between tubulinopathies and ciliopathies.
Subject(s)
Axoneme , Centrioles , Cilia , Ciliary Motility Disorders , Tubulin , Animals , Humans , Mice , Axoneme/metabolism , Centrioles/metabolism , Cilia/metabolism , Ciliary Motility Disorders/genetics , Ciliary Motility Disorders/metabolism , Mutation , Protein Isoforms/genetics , Protein Isoforms/metabolism , Tubulin/genetics , Tubulin/metabolism , Male , Female , Mice, KnockoutABSTRACT
Background: The purpose of this study is to explore the effect of vitamin B complex supplementation following periodontal flap surgery on clinical and microbiological parameters. Materials and Methods: A randomized controlled trial on 10 patients with periodontitis in split-mouth design was undertaken to find the effect of vitamin B complex supplementation with open flap debridement on periodontal wound healing. Multiplex polymerase chain reaction (PCR) for Tannerella forsythus and Porphyromonas gingivalis was done using subgingival plaque samples at 0 and 90th day. Results: The results showed a significant reduction (P < 0.01) of clinical (plaque index, gingival index, gingival bleeding index, probing pocket depth, and relative attachment level) and microbial profile in both treatment groups, whereas on intergroup analysis, more reduction in all clinical parameters were observed in the test group, but statistically, the results were insignificant.
ABSTRACT
Recent research shows that integrin-mediated adhesion contributes to the regulation of cell division at two key steps: the formation of the mitotic spindle at the mitotic entry and the final cytokinetic abscission at the mitotic exit. Failure in either of these processes will have a direct impact on the other in each round of the cell cycle and on the genomic integrity. This review aims to present how integrin signals are involved at these cell cycle stages under normal conditions and some safety mechanisms that may counteract the generation of aneuploid cells in cases of defective integrin signals.
ABSTRACT
PURPOSE: To investigate the factors influencing patients' willingness to replace removable dentures using structural equation modeling (SEM). METHODS: A total of 153 patients who sought consultations for removable dentures self-evaluated denture quality using a visual analog scale (VAS); health-related quality of life using the 36-item Short-Form Health Survey; and oral health-related quality of life using the Oral Health Impact Profile. Dental clinicians evaluated denture quality using a VAS and by assessing the presence of defects. After being informed of various treatments, patients were asked whether they would prefer denture replacement. SEM was applied to analyze the relationships and interactive effects among the variables. RESULTS: The final model showed high goodness-of-fit indices (chi-square/degree of freedom = 1.009, comparative fit index = 1.000, Tucker-Lewis index = 0.999, standardized root mean square residual = 0.421, and root mean square error of approximation = 0.008). SEM demonstrated that two latent constructs indirectly predicted patients' willingness to replace dentures; the standardized total effects of good oral health and poor denture quality were -0.154 and 0.503, respectively. CONCLUSION: These findings provide a unified understanding of the shared decision-making process for denture replacement and highlight the pretreatment assessments that play a relevant role in patient treatment preferences.