Comparison of a Novel Score "NOD-ACLF" to Other Established Prognostic Scores for Prediction of Mortality in APASL-ACLF Patients: A Cohort Study from a Tertiary Care Center of North India.

Background: Commonly used prognostic scores for acute on-chronic liver failure (ACLF) have complex calculations. We tried to compare the simple counting of numbers and types of organ dysfunction to these scores, to predict mortality in ACLF patients. Methods: In this prospective cohort study, ACLF patients diagnosed on the basis of Asia Pacific Association for Study of the Liver (APASL) definition were included. Severity scores were calculated. Prognostic factors for outcome were analysed. A new score, the Number of Organ Dysfunctions in Acute-on-Chronic Liver Failure (NOD-ACLF) score was developed. Results: Among 80 ACLF patients, 74 (92.5%) were male, and 6 were female (7.5%). The mean age was 41.0±10.7 (18-70) years. Profile of acute insult was; alcohol 48 (60%), sepsis 30 (37.5%), variceal bleeding 22 (27.5%), viral 8 (10%), and drug-induced 3 (3.8%). Profiles of chronic insults were alcohol 61 (76.3%), viral 20 (25%), autoimmune 3 (3.8%), and non-alcoholic steatohepatitis 2 (2.5%). Thirty-eight (47.5%) were discharged, and 42 (52.5%) expired. The mean number of organ dysfunction (NOD-ACLF score) was ->4.5, simple organ failure count (SOFC) score was >2.5, APASL ACLF Research Consortium score was >11.5, Model for End-Stage Liver Disease-Lactate (MELD-LA) score was >21.5, and presence of cardiovascular and respiratory dysfunctions were significantly associated with mortality. NOD-ACLF and SOFC scores had the highest area under the receiver operating characteristic to predict mortality among all these. Conclusion: The NOD-ACLF score is easy to calculate bedside and is a good predictor of mortality in ACLF patients performing similar or better to other scores.

Health care workers' concerns during COVID-19 times.

Ground -level health care workers provide us a unique perspective of immunization programs. While our official data may be too rosy to be true, a casual / unscheduled /anonymous conversation with such foot- soldiers may bring us face -to- face with reality through another angle If we want our programs to be robust having a wide coverage, Mantra of success lies not only in making good policy while sitting in ivory -towers but also in folding our sleeves up, sweating under the tropical hot Sun, going to hinterlands and initiating a dialogue with those humble low- level workers who sometimes are invisible to us otherwise, not listened to and with whom sometimes promises are not kept. Promised amount for achieving some target may be small for us but for them that may not necessarily be true. Let's just enter their shoes for a while and try to observe the situation from their point -of- view. In our siloed system of administration when everyone has knowledge of his/ her territory, let us share our wisdom and field -experience. As an expert in one field may not be having much knowledge about another one.

Solving crimes, balancing rights in police investigation.

Jinee Lokneeta's editorial on Police investigation and unethical "scientific interrogation" was published in the January-March 2023 issue of IJME [1]. It is a scathing critique of the way police investigators rampantly misuse/exploit loopholes in the law, extract forced confessions from the accused and use them in a court of law - sometimes leading to convictions or prolonged incarceration of innocent victims. Her Excellency, the Hon'ble President of India, expressed similar sentiments when she questioned the need for building more jails at the same time that we talk of "moving towards progress as a society" [2]. Her comment was in the context of a large number of undertrials in jails, suffering from the inefficiency of the present day criminal justice system. Therefore, the need of the hour is to fix the weaknesses in the system and advance towards a rapid, truthful, honest and impartial system of police investigation. It is against this background that the journal has published the Editorial, and we support the broader intent which impelled the author to research the current criminal investigation system and expose its deficiencies. Nevertheless, when we go deeper into the details, several features begin to appear which seem incongruous with the author's arguments in her editorial.

Descriptive Study to Assess Post-acute COVID-19 Complications in Patients Presenting at a Teaching Hospital in North India.

Introduction Coronavirus disease 2019 (COVID-19) caused by a novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was first reported at the end of 2019 in Wuhan, Hubei Province, People's Republic of China, at a cluster of unusual pneumonia patients. The outbreak was declared a Public Health Emergency of International Concern on 30 January 2020 by World Health Organization. We are receiving patients in our OPD (Out Patient Department) with a new set of health complications having been infected with COVID-19. We planned to collect our data and try to find by various statistical methods, quantify the complications, and assess how we can deal with the new set of complications we are witnessing in this post-acute COVID-19 group of patients. Materials and methods The study was conducted by enrolling the patients at OPD/IPD (In Patient Department) by conducting a detailed history and clinical examination, routine investigations, 2D echocardiography (2D Echo), and pulmonary function test (PFT). The study assessed the worsening of symptoms, new onset symptoms, or the symptoms that continued even in the post-COVID-19 status as post-COVID-19 sequelae. Results Maximum cases were male and most of them were asymptomatic. The most common post-COVID-19 symptom that persisted was fatigue. 2D Echo and spirometry were done and changes were noticed even in those subjects who were asymptomatic. Conclusion Since significant findings were seen on clinical evaluation accompanied by 2D Echo and spirometry, it is essential to screen all presumed and microbiologically proven cases for long-term follow-up.

To Study the ß-Globin Haplotype Pattern of Descent of a Set of Linked Alleles Occurring on the Same Chromosome in the Northern Province of India.

OBJECTIVE: To study the five mutations commonly prevalent in North India, i.e., IVS-I-5 (G→C), 619 bp deletion, IVS-I-1 (G→T), codon 41/42 (-TTCT), and codon 8/9 (+G), in the beta thalassemia (ß-thalassemia) major children. The specific ß-thalassemia mutations of different haplotype patterns of the ß-globin gene cluster will also be determined. METHODS: A total of 125 children diagnosed with ß-thalassemia major visiting the Department of Pediatrics of King George's Medical University were involved in the study. As per the QIAamp (Qiagen, Hilden, Germany) manufacturer guidelines, genomic DNA was isolated from whole blood. To identify the haplotype pattern within the ß-globin gene cluster, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used. The respective restriction endonucleases used were Hind III/GƔ, Hinc II/Ψß, Hinf I/ß, Ava II/ß, and BamHI for the haplotype analysis in the ß-globin pattern of descent of a set of linked alleles occurring on the same chromosome. RESULTS: Among the five common mutations, 73 patients had IVS-I-5 (G→C), 28 patients had 619 bp deletion, 17 patients had IVS-I-1 (G→T), five patients had Cd 41/42 (-TTCT), and two patients had Cd 8/9 (+G) mutations. Fifteen haplotypes (haplotypes 1-15) were identified in 125 ß-thalassemia major children. Among the five haplotypes observed in the IVS-I-5 (G→C) mutation, the H1 haplotype was most predominant with a frequency of 27.2%, followed by the H2, H4, H3, and H10 haplotypes in the given population. In 619 bp deletion, IVS-I-1 (G→T), codon 41/42, and codon 8/9, haplotype H9, H12, H11, and H5 were seen, respectively. CONCLUSION: ß-thalassemia was found to be the most common in the northern province of Uttar Pradesh. The linkage of ß-globin gene haplotypes with ß-thalassemia mutations was explored in the northern province of Uttar Pradesh. The population of different natives is being mixed up due to migration and industrialization. These were some reasons for the occurrence of haplotypic heterogeneity. This haplotype heterogeneity was correlated with the origin of these mutations found to be unlike the origin of common ones from different provinces.